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HAND1 heart and neural crest derivatives expressed 1 [Homo sapiens (human)]

Gene Symbol HAND1
Entrez Gene ID 9421
Full Name heart and neural crest derivatives expressed 1
Synonyms Hxt, Thing1, bHLHa27, eHand
General protein information
Preferred Names
heart- and neural crest derivatives-expressed protein 1
Names
heart- and neural crest derivatives-expressed protein 1
class A basic helix-loop-helix protein 27
extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q33

Summary The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

602406

Disorder Html:
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OHu39917 XM_005268531 PREDICTED: Homo sapiens heart and neural crest derivatives expressed 1 (HAND1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK Not in stock -1 Starting from $99.00
OHu26705 NM_004821 Homo sapiens heart and neural crest derivatives expressed 1 (HAND1), mRNA. pcDNA3.1-C-(k)DYK In stock -1 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OHu39917D
Sequence Information ORF Nucleotide Sequence (Length: 645bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product heart- and neural crest derivatives-expressed protein 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029289.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACCCGCA CCCTGCGCAC 
CCCATGCTCC ACGAACCCTT CCTCTTCGGT CCGGCCTCGC GCTGTCATCA GGAAAGGCCC
TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GACGCTGCCC CGGACTTCCC TGCGGGCGGG
CCGCCGCCCG CGGCCGCTGC AGCCGCCACC GCCTATGGTC CTGACGCCAG GCCTGGGCAG
AGCCCCGGGC GGCTGGAGGC GCTTGGCGGC CGTCTTGGCC GGCGGAAAGG CTCAGGACCC
AAGAAGGAGC GGAGACGCAC TGAGAGCATT AACAGCGCAT TCGCGGAGTT GCGCGAGTGC
ATCCCCAACG TGCCGGCCGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTAGCCACC
AGCTACATCG CCTACCTGAT GGACGTGCTG GCCAAGGATG CACAGTCTGG CGATCCCGAG
GCCTTCAAGG CTGAACTCAA GAAGGCGGAT GGCGGCCGTG AGAGCAAGCG GAAAAGGGAG
CTGCAGCACG AAGGTTTTCC TCCTGCCCTG GGCCCAGTCG AGAAGAGGAT TAAAGGACGC
ACCGGCTGGC CGCAGCAAGT CTGGGCGCTG GAGTTAAACC AGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqXP_005268588.1
CDS202..846
Misc Feature(1)478..639(+)
Misc Feature(2)484..597(+)
Misc Feature(3)508..510(+)
Misc Feature(4)526..630(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:3822714
10 10 c, t dbSNP:578193925
74 74 g, t dbSNP:993098
130 130 c, t dbSNP:181499341
155 155 a, g dbSNP:201748550
158 158 a, c, t dbSNP:781476191
161 161 c, t dbSNP:549580359
165 165 a, t dbSNP:111403472
167 167 c, t dbSNP:376145648
175 175 a, g dbSNP:13171812
176 176 a, c dbSNP:374096842
178 178 a, g dbSNP:779569406
184 184 c, g dbSNP:758014034
187 187 a, c dbSNP:749996768
188 188 g, t dbSNP:764963596
190 190 a, c dbSNP:754050287
197 197 c, g, t dbSNP:753667174
202 202 a, g dbSNP:763806635
207 207 c, t dbSNP:760607439
210 210 c, t dbSNP:775319214
211 211 a, g dbSNP:767528053
219 219 c, g dbSNP:762997931
223 223 a, g, t dbSNP:371033200
235 235 c, t dbSNP:570134327
238 238 c, t dbSNP:769999347
247 247 -, atcaccacc dbSNP:754696027
247 247 a, c dbSNP:200556971
249 249 a, g dbSNP:776997898
250 250 c, t dbSNP:768925214
257 257 c, g dbSNP:747513609
263 263 c, g dbSNP:780396101
272 272 a, g dbSNP:772757234
274 274 a, g dbSNP:745362462
277 277 c, t dbSNP:528298246
288 288 a, c dbSNP:756908501
289 289 a, g dbSNP:753450334
293 293 a, c dbSNP:777581629
297 297 c, t dbSNP:755831294
300 300 a, g dbSNP:373192317
311 311 a, g dbSNP:533319519
312 312 c, g dbSNP:759535790
323 323 a, c, g dbSNP:564241048
325 325 c, t dbSNP:762046238
327 327 c, g dbSNP:776751181
329 329 a, g dbSNP:768965463
333 333 c, g, t dbSNP:145232171
335 335 c, g dbSNP:141689288
336 336 g, t dbSNP:772706064
337 337 a, c dbSNP:746385291
343 343 a, g dbSNP:778513187
345 345 c, g dbSNP:770592263
347 347 c, g dbSNP:748866943
355 355 a, g dbSNP:777266765
360 360 c, g dbSNP:755852180
362 362 c, g, t dbSNP:780969396
363 363 a, g dbSNP:138460675
374 374 a, c dbSNP:201496181
375 375 a, c, g dbSNP:761768832
376 376 a, g dbSNP:754000582
379 379 c, g dbSNP:202045612
380 380 a, g dbSNP:761028320
381 381 a, g dbSNP:369386099
382 382 c, t dbSNP:768012504
383 383 c, t dbSNP:760076464
384 384 a, g dbSNP:774965575
387 387 a, g, t dbSNP:748732968
388 388 c, t dbSNP:772843786
389 389 c, t dbSNP:769262740
390 390 c, g dbSNP:201149780
396 396 c, g dbSNP:747850568
401 401 c, t dbSNP:780916501
403 403 c, g dbSNP:374965548
405 405 c, t dbSNP:746848071
409 409 a, c dbSNP:576059307
412 412 a, g, t dbSNP:758422053
414 414 a, c dbSNP:753861203
416 416 a, g dbSNP:764242373
418 418 a, g dbSNP:756237060
420 420 c, t dbSNP:6880185
426 426 c, g dbSNP:752995620
427 427 a, g dbSNP:767888040
440 440 a, g dbSNP:201037401
441 441 c, g dbSNP:774910795
442 442 a, t dbSNP:766789740
443 443 c, g dbSNP:763518945
446 446 c, t dbSNP:772538065
448 448 g, t dbSNP:201302313
453 453 a, g dbSNP:576744103
462 462 c, g, t dbSNP:768347498
468 468 a, c, t dbSNP:779893795
472 472 c, g dbSNP:369296058
480 480 c, t dbSNP:201302137
481 481 c, t dbSNP:777670897
484 484 c, t dbSNP:756183625
485 485 a, g dbSNP:752869371
487 487 a, g dbSNP:572764589
491 491 -, g dbSNP:753714358
497 497 c, g, t dbSNP:751904421
501 501 c, t dbSNP:766779838
505 505 a, g dbSNP:763319157
509 509 -, ctga dbSNP:758433854
513 513 a, g dbSNP:752927590
515 515 g, t dbSNP:765342459
516 516 a, g dbSNP:759848673
528 528 c, t dbSNP:556045294
531 531 c, t dbSNP:765703979
535 535 a, t dbSNP:761291470
537 537 a, c dbSNP:776179261
540 540 a, g dbSNP:150104384
543 543 a, c dbSNP:774267927
552 552 g, t dbSNP:536000696
558 558 c, g dbSNP:775093878
561 561 c, t dbSNP:771928311
571 571 g, t dbSNP:745686103
579 579 a, c dbSNP:778884559
607 607 -, ct dbSNP:765936211
610 610 a, c dbSNP:771047758
611 611 a, g, t dbSNP:374536409
619 619 a, g dbSNP:755192128
631 631 a, g, t dbSNP:140560109
633 633 c, g dbSNP:570062952
644 644 a, g dbSNP:750803617
648 648 a, g dbSNP:765762725
655 655 a, g dbSNP:762375465
669 669 g, t dbSNP:34402828
672 672 a, c dbSNP:763611267
693 693 g, t dbSNP:760275585
701 701 a, g, t dbSNP:202139442
702 702 a, g dbSNP:759358821
708 708 a, g dbSNP:774078948
713 713 a, g dbSNP:201984942
719 719 g, t dbSNP:146912397
722 722 a, g dbSNP:781370812
730 730 a, c dbSNP:768888751
731 731 a, g dbSNP:747099689
732 732 c, g dbSNP:34198899
747 747 a, g dbSNP:779223514
759 759 c, t dbSNP:757655487
764 764 c, t dbSNP:147677474
766 766 g, t dbSNP:778198835
767 767 c, t dbSNP:756586663
774 774 c, t dbSNP:752250641
779 779 c, t dbSNP:373277337
781 781 c, g dbSNP:762914657
785 785 -, aga dbSNP:767079781
793 793 a, c dbSNP:754637839
797 797 a, g dbSNP:751211164
803 803 c, g dbSNP:766161267
806 806 c, g dbSNP:762786810
807 807 c, g dbSNP:773154099
812 812 a, c dbSNP:765198552
813 813 c, g, t dbSNP:368908340
820 820 c, g dbSNP:772091154
827 827 a, c dbSNP:746068193
834 834 a, g dbSNP:774512591
841 841 c, t dbSNP:771160670
845 845 a, g dbSNP:749591449
847 847 a, g dbSNP:778180370
850 850 a, g dbSNP:756633155
856 856 c, g, t dbSNP:780723638
857 857 a, g dbSNP:201337632
862 862 a, g, t dbSNP:779794386
864 864 c, g dbSNP:374569248
867 867 c, t dbSNP:370439489
868 868 c, t dbSNP:750237915
872 872 c, t dbSNP:764997341
876 876 c, g dbSNP:761786357
890 890 a, g dbSNP:753756243
893 893 c, t dbSNP:376440284
929 929 c, t dbSNP:377327099
947 947 g, t dbSNP:569052472
955 955 g, t dbSNP:186778831
1002 1002 c, t dbSNP:538799782
1044 1044 a, t dbSNP:181136691
1050 1050 a, g dbSNP:375335104
1056 1056 a, g dbSNP:139139273
1075 1075 -, at dbSNP:754947682
1076 1076 -, at dbSNP:772211792
1077 1077 -, at dbSNP:528518569
1111 1111 a, g dbSNP:560973762
1124 1124 -, aag dbSNP:779259287
1163 1163 c, t dbSNP:189652687
1178 1178 a, g dbSNP:564072656
1180 1180 a, c dbSNP:532078892
1187 1187 a, g dbSNP:765063325
1227 1227 c, t dbSNP:562993925
1229 1229 -, ctct dbSNP:561201958
1231 1231 c, g dbSNP:778026920
1280 1280 a, g dbSNP:370530933
1291 1291 a, t dbSNP:377533464
1304 1304 g, t dbSNP:759312194
1339 1339 a, g dbSNP:776355630
1340 1340 a, g dbSNP:150779203
1361 1361 c, g dbSNP:576994472
1367 1367 a, g dbSNP:770719086
1388 1388 a, t dbSNP:760212975
1439 1439 -, ct dbSNP:377024721
1445 1445 c, t dbSNP:184353195
1498 1498 a, t dbSNP:35542711
1506 1506 a, g dbSNP:748150571
1655 1655 c, g dbSNP:779103553

Target ORF information:

RefSeq Version XM_005268531
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens heart and neural crest derivatives expressed 1 (HAND1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
XM_005268531

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACCCGCA CCCTGCGCAC 
CCCATGCTCC ACGAACCCTT CCTCTTCGGT CCGGCCTCGC GCTGTCATCA GGAAAGGCCC
TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GACGCTGCCC CGGACTTCCC TGCGGGCGGG
CCGCCGCCCG CGGCCGCTGC AGCCGCCACC GCCTATGGTC CTGACGCCAG GCCTGGGCAG
AGCCCCGGGC GGCTGGAGGC GCTTGGCGGC CGTCTTGGCC GGCGGAAAGG CTCAGGACCC
AAGAAGGAGC GGAGACGCAC TGAGAGCATT AACAGCGCAT TCGCGGAGTT GCGCGAGTGC
ATCCCCAACG TGCCGGCCGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTAGCCACC
AGCTACATCG CCTACCTGAT GGACGTGCTG GCCAAGGATG CACAGTCTGG CGATCCCGAG
GCCTTCAAGG CTGAACTCAA GAAGGCGGAT GGCGGCCGTG AGAGCAAGCG GAAAAGGGAG
CTGCAGCACG AAGGTTTTCC TCCTGCCCTG GGCCCAGTCG AGAAGAGGAT TAAAGGACGC
ACCGGCTGGC CGCAGCAAGT CTGGGCGCTG GAGTTAAACC AGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OHu26705D
Sequence Information ORF Nucleotide Sequence (Length: 648bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product heart- and neural crest derivatives-expressed protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF061756.1. On Aug 16, 2008 this sequence version replaced gi:4758505. Summary: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF061756.1, AL524188.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2153932 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACCCGCA CCCTGCGCAC 
CCCATGCTCC ACGAACCCTT CCTCTTCGGT CCGGCCTCGC GCTGTCATCA GGAAAGGCCC
TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GACGCTGCCC CGGACTTCCC TGCGGGCGGG
CCGCCGCCCG CGGCCGCTGC AGCCGCCACC GCCTATGGTC CTGACGCCAG GCCTGGGCAG
AGCCCCGGGC GGCTGGAGGC GCTTGGCGGC CGTCTTGGCC GGCGGAAAGG CTCAGGACCC
AAGAAGGAGC GGAGACGCAC TGAGAGCATT AACAGCGCAT TCGCGGAGTT GCGCGAGTGC
ATCCCCAACG TGCCGGCCGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTAGCCACC
AGCTACATCG CCTACCTGAT GGACGTGCTG GCCAAGGATG CACAGTCTGG CGATCCCGAG
GCCTTCAAGG CTGAACTCAA GAAGGCGGAT GGCGGCCGTG AGAGCAAGCG GAAAAGGGAG
CTGCAGCAGC ACGAAGGTTT TCCTCCTGCC CTGGGCCCAG TCGAGAAGAG GATTAAAGGA
CGCACCGGCT GGCCGCAGCA AGTCTGGGCG CTGGAGTTAA ACCAGTGA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_004812.1
CDS257..904
Misc Feature(1)533..694(+)
Misc Feature(2)539..652(+)
Misc Feature(3)548..550(+)
Misc Feature(4)548..550(+)
Misc Feature(5)563..565(+)
Misc Feature(6)575..577(+)
Misc Feature(7)575..577(+)
Misc Feature(8)575..577(+)
Misc Feature(9)581..685(+)
Misc Feature(10)581..583(+)
Misc Feature(11)581..583(+)
Misc Feature(12)581..583(+)
Exon (1)1..799
Gene:HAND1
Gene Synonym:
Exon (2)800..1738
Gene:HAND1
Gene Synonym:
Translation
Position Chain Variation Link
15 15 g, t dbSNP:563875535
23 23 c, t dbSNP:115396154
38 38 a, c dbSNP:555932965
56 56 c, t dbSNP:3822714
65 65 c, t dbSNP:578193925
129 129 g, t dbSNP:993098
185 185 c, t dbSNP:181499341
210 210 a, g dbSNP:201748550
213 213 a, c, t dbSNP:781476191
216 216 c, t dbSNP:549580359
220 220 a, t dbSNP:111403472
222 222 c, t dbSNP:376145648
230 230 a, g dbSNP:13171812
231 231 a, c dbSNP:374096842
233 233 a, g dbSNP:779569406
239 239 c, g dbSNP:758014034
242 242 a, c dbSNP:749996768
243 243 g, t dbSNP:764963596
245 245 a, c dbSNP:754050287
252 252 c, g, t dbSNP:753667174
257 257 a, g dbSNP:763806635
262 262 c, t dbSNP:760607439
265 265 c, t dbSNP:775319214
266 266 a, g dbSNP:767528053
274 274 c, g dbSNP:762997931
278 278 a, g, t dbSNP:371033200
290 290 c, t dbSNP:570134327
293 293 c, t dbSNP:769999347
302 302 -, atcaccacc dbSNP:754696027
302 302 a, c dbSNP:200556971
304 304 a, g dbSNP:776997898
305 305 c, t dbSNP:768925214
312 312 c, g dbSNP:747513609
318 318 c, g dbSNP:780396101
327 327 a, g dbSNP:772757234
329 329 a, g dbSNP:745362462
332 332 c, t dbSNP:528298246
343 343 a, c dbSNP:756908501
344 344 a, g dbSNP:753450334
348 348 a, c dbSNP:777581629
352 352 c, t dbSNP:755831294
355 355 a, g dbSNP:373192317
366 366 a, g dbSNP:533319519
367 367 c, g dbSNP:759535790
378 378 a, c, g dbSNP:564241048
380 380 c, t dbSNP:762046238
382 382 c, g dbSNP:776751181
384 384 a, g dbSNP:768965463
388 388 c, g, t dbSNP:145232171
390 390 c, g dbSNP:141689288
391 391 g, t dbSNP:772706064
392 392 a, c dbSNP:746385291
398 398 a, g dbSNP:778513187
400 400 c, g dbSNP:770592263
402 402 c, g dbSNP:748866943
410 410 a, g dbSNP:777266765
415 415 c, g dbSNP:755852180
417 417 c, g, t dbSNP:780969396
418 418 a, g dbSNP:138460675
429 429 a, c dbSNP:201496181
430 430 a, c, g dbSNP:761768832
431 431 a, g dbSNP:754000582
434 434 c, g dbSNP:202045612
435 435 a, g dbSNP:761028320
436 436 a, g dbSNP:369386099
437 437 c, t dbSNP:768012504
438 438 c, t dbSNP:760076464
439 439 a, g dbSNP:774965575
442 442 a, g, t dbSNP:748732968
443 443 c, t dbSNP:772843786
444 444 c, t dbSNP:769262740
445 445 c, g dbSNP:201149780
451 451 c, g dbSNP:747850568
456 456 c, t dbSNP:780916501
458 458 c, g dbSNP:374965548
460 460 c, t dbSNP:746848071
464 464 a, c dbSNP:576059307
467 467 a, g, t dbSNP:758422053
469 469 a, c dbSNP:753861203
471 471 a, g dbSNP:764242373
473 473 a, g dbSNP:756237060
475 475 c, t dbSNP:6880185
481 481 c, g dbSNP:752995620
482 482 a, g dbSNP:767888040
495 495 a, g dbSNP:201037401
496 496 c, g dbSNP:774910795
497 497 a, t dbSNP:766789740
498 498 c, g dbSNP:763518945
501 501 c, t dbSNP:772538065
503 503 g, t dbSNP:201302313
508 508 a, g dbSNP:576744103
517 517 c, g, t dbSNP:768347498
523 523 a, c, t dbSNP:779893795
527 527 c, g dbSNP:369296058
535 535 c, t dbSNP:201302137
536 536 c, t dbSNP:777670897
539 539 c, t dbSNP:756183625
540 540 a, g dbSNP:752869371
542 542 a, g dbSNP:572764589
546 546 -, g dbSNP:753714358
552 552 c, g, t dbSNP:751904421
556 556 c, t dbSNP:766779838
560 560 a, g dbSNP:763319157
564 564 -, ctga dbSNP:758433854
568 568 a, g dbSNP:752927590
570 570 g, t dbSNP:765342459
571 571 a, g dbSNP:759848673
583 583 c, t dbSNP:556045294
586 586 c, t dbSNP:765703979
590 590 a, t dbSNP:761291470
592 592 a, c dbSNP:776179261
595 595 a, g dbSNP:150104384
598 598 a, c dbSNP:774267927
607 607 g, t dbSNP:536000696
613 613 c, g dbSNP:775093878
616 616 c, t dbSNP:771928311
626 626 g, t dbSNP:745686103
634 634 a, c dbSNP:778884559
662 662 -, ct dbSNP:765936211
665 665 a, c dbSNP:771047758
666 666 a, g, t dbSNP:374536409
674 674 a, g dbSNP:755192128
686 686 a, g, t dbSNP:140560109
688 688 c, g dbSNP:570062952
699 699 a, g dbSNP:750803617
703 703 a, g dbSNP:765762725
710 710 a, g dbSNP:762375465
724 724 g, t dbSNP:34402828
727 727 a, c dbSNP:763611267
748 748 g, t dbSNP:760275585
756 756 a, g, t dbSNP:202139442
757 757 a, g dbSNP:759358821
763 763 a, g dbSNP:774078948
768 768 a, g dbSNP:201984942
774 774 g, t dbSNP:146912397
777 777 a, g dbSNP:781370812
785 785 a, c dbSNP:768888751
786 786 a, g dbSNP:747099689
787 787 c, g dbSNP:34198899
805 805 a, g dbSNP:779223514
817 817 c, t dbSNP:757655487
822 822 c, t dbSNP:147677474
824 824 g, t dbSNP:778198835
825 825 c, t dbSNP:756586663
832 832 c, t dbSNP:752250641
837 837 c, t dbSNP:373277337
839 839 c, g dbSNP:762914657
843 843 -, aga dbSNP:767079781
851 851 a, c dbSNP:754637839
855 855 a, g dbSNP:751211164
861 861 c, g dbSNP:766161267
864 864 c, g dbSNP:762786810
865 865 c, g dbSNP:773154099
870 870 a, c dbSNP:765198552
871 871 c, g, t dbSNP:368908340
878 878 c, g dbSNP:772091154
885 885 a, c dbSNP:746068193
892 892 a, g dbSNP:774512591
899 899 c, t dbSNP:771160670
903 903 a, g dbSNP:749591449
905 905 a, g dbSNP:778180370
908 908 a, g dbSNP:756633155
914 914 c, g, t dbSNP:780723638
915 915 a, g dbSNP:201337632
920 920 a, g, t dbSNP:779794386
922 922 c, g dbSNP:374569248
925 925 c, t dbSNP:370439489
926 926 c, t dbSNP:750237915
930 930 c, t dbSNP:764997341
934 934 c, g dbSNP:761786357
948 948 a, g dbSNP:753756243
951 951 c, t dbSNP:376440284
987 987 c, t dbSNP:377327099
1005 1005 g, t dbSNP:569052472
1013 1013 g, t dbSNP:186778831
1060 1060 c, t dbSNP:538799782
1102 1102 a, t dbSNP:181136691
1108 1108 a, g dbSNP:375335104
1114 1114 a, g dbSNP:139139273
1133 1133 -, at dbSNP:754947682
1134 1134 -, at dbSNP:772211792
1135 1135 -, at dbSNP:528518569
1169 1169 a, g dbSNP:560973762
1182 1182 -, aag dbSNP:779259287
1221 1221 c, t dbSNP:189652687
1236 1236 a, g dbSNP:564072656
1238 1238 a, c dbSNP:532078892
1245 1245 a, g dbSNP:765063325
1285 1285 c, t dbSNP:562993925
1287 1287 -, ctct dbSNP:561201958
1289 1289 c, g dbSNP:778026920
1338 1338 a, g dbSNP:370530933
1349 1349 a, t dbSNP:377533464
1362 1362 g, t dbSNP:759312194
1397 1397 a, g dbSNP:776355630
1398 1398 a, g dbSNP:150779203
1419 1419 c, g dbSNP:576994472
1425 1425 a, g dbSNP:770719086
1446 1446 a, t dbSNP:760212975
1497 1497 -, ct dbSNP:377024721
1503 1503 c, t dbSNP:184353195
1556 1556 a, t dbSNP:35542711
1564 1564 a, g dbSNP:748150571
1713 1713 c, g dbSNP:779103553

Target ORF information:

RefSeq Version NM_004821
Organism Homo sapiens (human)
Definition Homo sapiens heart and neural crest derivatives expressed 1 (HAND1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_004821

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACCCGCA CCCTGCGCAC 
CCCATGCTCC ACGAACCCTT CCTCTTCGGT CCGGCCTCGC GCTGTCATCA GGAAAGGCCC
TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GACGCTGCCC CGGACTTCCC TGCGGGCGGG
CCGCCGCCCG CGGCCGCTGC AGCCGCCACC GCCTATGGTC CTGACGCCAG GCCTGGGCAG
AGCCCCGGGC GGCTGGAGGC GCTTGGCGGC CGTCTTGGCC GGCGGAAAGG CTCAGGACCC
AAGAAGGAGC GGAGACGCAC TGAGAGCATT AACAGCGCAT TCGCGGAGTT GCGCGAGTGC
ATCCCCAACG TGCCGGCCGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTAGCCACC
AGCTACATCG CCTACCTGAT GGACGTGCTG GCCAAGGATG CACAGTCTGG CGATCCCGAG
GCCTTCAAGG CTGAACTCAA GAAGGCGGAT GGCGGCCGTG AGAGCAAGCG GAAAAGGGAG
CTGCAGCAGC ACGAAGGTTT TCCTCCTGCC CTGGGCCCAG TCGAGAAGAG GATTAAAGGA
CGCACCGGCT GGCCGCAGCA AGTCTGGGCG CTGGAGTTAA ACCAGTGA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.