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TBX4 T-box 4 [Homo sapiens (human)]

Gene Symbol TBX4
Entrez Gene ID 9496
Full Name T-box 4
Synonyms SPS
General protein information
Preferred Names
T-box transcription factor TBX4
Names
T-box transcription factor TBX4
T-box protein 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

17

17q21-q22

Summary This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

601719

Disorder Html: Small patella syndrome, 147891 (3)





The following TBX4 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBX4 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu62873 XM_011525490 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu62874 XM_011525491 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu50822 XM_005257835 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu50822 XM_005257837 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu50822 XM_011525492 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu50822 XM_011525493 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu50822 XM_011525494 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439
OHu62875 XM_011525495 PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $319
OHu18883 NM_018488 Homo sapiens T-box 4 (TBX4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $439

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu62873
Accession Version XM_011525490.1
Sequence Information ORF Nucleotide Sequence (Length: 1830bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGGTTTTT CCCAACCCAG ACTGGCCCCG GCCCCTTTTT ATCTCCTGGG TTGCACTTGG 
CTTGTTTACT TTGCGAGGTC CTGGGAGTCG AAGTCTAAGC CCGGGATGAG GAGGCCTCTG
AACAAGATGG GCGAGAGGCC TGAGCCCGGG GATGCTCCGG GAGGGCCCGG GGAGAGCGGG
AAGAAGGAGA TGCTGCAGGA TAAGGGCCTG TCCGAGAGCG AGGAGGCCTT CCGGGCCCCG
GGCCCAGCGC TCGGAGAGGC CAGCGCAGCC AACGCCCCCG AGCCCGCGCT GGCAGCGCCG
GGCCTCAGCG GAGCCGCGCT AGGCAGCCCC CCGGGACCCG GGGCCGACGT CGTCGCCGCC
GCCGCCGCGG AGCAGACCAT CGAGAACATC AAGGTGGGGC TGCATGAGAA GGAGCTCTGG
AAGAAGTTCC ACGAGGCGGG CACCGAGATG ATCATCACTA AGGCTGGCAG GAGGATGTTC
CCCAGCTACA AGGTAAAAGT CACAGGCATG AACCCCAAGA CCAAGTATAT CCTGCTGATT
GACATTGTCC CTGCCGATGA CCATCGCTAC AAGTTCTGTG ACAACAAATG GATGGTGGCA
GGGAAGGCTG AGCCAGCCAT GCCAGGAAGG CTGTATGTCC ACCCGGATTC TCCTGCCACA
GGAGCCCACT GGATGCGGCA GCTGGTCTCC TTCCAGAAGC TGAAGCTGAC AAACAACCAC
CTGGACCCCT TTGGCCATAT CATCCTCAAC TCTATGCACA AGTACCAGCC GCGGCTCCAC
ATCGTTAAGG CTGATGAGAA CAATGCTTTC GGCTCCAAAA ACACTGCTTT CTGCACCCAC
GTGTTCCCAG AGACCTCCTT CATCTCTGTG ACCTCCTACC AGAATCACAA GATCACCCAG
CTGAAAATTG AGAACAACCC TTTTGCCAAG GGATTCCGGG GCAGTGATGA CAGTGACCTG
CGTGTGGCCC GACTGCAGAG CAAAGAATAC CCCGTGATTT CCAAAAGCAT CATGAGGCAG
AGGCTCATCT CCCCCCAGCT CTCAGCCACA CCGGACGTGG GCCCCCTGCT CGGCACCCAC
CAGGCACTCC AGCACTACCA GCACGAGAAC GGGGCACACT CACAGCTCGC GGAGCCGCAG
GACCTGCCCC TCAGCACCTT TCCCACCCAG AGGGACTCAA GCCTCTTCTA TCACTGCCTG
AAAAGACGAG CAGACGGTAC CCGCCACCTG GACTTACCTT GCAAGCGATC CTATCTGGAA
GCCCCCTCTT CGGTGGGGGA GGATCACTAT TTCCGTTCCC CCCCTCCCTA CGACCAGCAA
ATGCTGAGCC CCTCCTACTG CAGTGAGGTG ACCCCCAGAG AAGCATGTAT GTACTCAGGT
TCAGGGCCCG AGATTGCCGG GGTGTCTGGG GTGGACGACC TGCCCCCACC TCCGCTGAGC
TGTAACATGT GGACTTCAGT GTCGCCGTAC ACCAGCTATA GCGTGCAGAC GATGGAGACT
GTGCCGTACC AGCCCTTCCC CACGCACTTC ACCGCCACCA CCATGATGCC GCGGCTGCCC
ACCCTCTCCG CTCAGAGCTC CCAGCCACCA GGAAATGCCC ACTTTAGTGT CTACAATCAG
CTCTCCCAGT CTCAGGTCCG AGAGCGGGGG CCCAGCGCCT CATTCCCAAG AGAGCGCGGC
CTCCCCCAAG GGTGTGAGAG GAAGCCACCC TCGCCACATC TAAATGCTGC CAATGAGTTT
CTCTACTCTC AAACCTTCTC CTTGTCCCGA GAATCTTCCT TACAGTACCA TTCAGGAATG
GGGACTGTGG AGAACTGGAC TGACGGATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011523792.1
CDS58..1887
Misc Feature(1)442..1011(+)
Misc Feature(2)508..999(+)
Misc Feature(3)580..849(+)
Translation
Position Chain Variation Link
45 45 a, c dbSNP:181750228
86 86 c, t dbSNP:114542698
103 103 c, t dbSNP:545336781
162 162 c, g dbSNP:555178103
173 173 c, t dbSNP:112225845
185 185 c, t dbSNP:775575406
190 190 a, g dbSNP:565702742
192 192 c, g dbSNP:535141245
204 204 c, t dbSNP:115284369
225 225 c, t dbSNP:760687344
228 228 g, t dbSNP:577978421
242 242 a, g dbSNP:543879893
259 259 -, a dbSNP:769433167
262 262 a, g dbSNP:117410176
263 263 c, g dbSNP:3744448
264 264 c, t dbSNP:752448330
269 269 c, t dbSNP:776660359
270 270 c, t dbSNP:777880490
271 271 c, g dbSNP:748755835
280 280 a, g dbSNP:756802394
287 287 c, t dbSNP:778604942
288 288 c, t dbSNP:745523181
289 289 c, t dbSNP:771639735
309 309 c, g dbSNP:775860775
310 310 c, g dbSNP:747369610
311 311 c, g dbSNP:769157857
317 317 c, t dbSNP:573485618
340 340 c, t dbSNP:762348865
349 349 g, t dbSNP:765299000
350 350 c, t dbSNP:148424252
392 392 g, t dbSNP:558901097
396 396 c, t dbSNP:763175971
400 400 a, g dbSNP:766513791
402 402 a, c dbSNP:752496226
405 405 c, t dbSNP:755880238
407 407 c, t dbSNP:764025450
411 411 -, cgc dbSNP:772891363
418 418 a, g dbSNP:575407008
421 421 a, g dbSNP:80164240
430 430 c, t dbSNP:104894648
436 436 a, t dbSNP:554741896
441 441 a, g dbSNP:759838845
453 453 c, g dbSNP:763974215
454 454 a, g dbSNP:753701583
455 455 a, g dbSNP:761779230
472 472 c, g dbSNP:765300595
480 480 g, t dbSNP:182844772
495 495 a, g dbSNP:35070263
501 501 a, c dbSNP:779655731
504 504 a, g dbSNP:751168165
509 509 c, t dbSNP:754685175
522 522 g, t dbSNP:3744447
552 552 a, g dbSNP:370004814
559 559 a, g dbSNP:753009856
562 562 a, g dbSNP:200429513
569 569 a, g dbSNP:778236633
573 573 c, t dbSNP:749751811
581 581 a, g dbSNP:377571192
589 589 c, t dbSNP:778794211
595 595 a, g dbSNP:745977352
599 599 a, g dbSNP:772372461
605 605 c, t dbSNP:200977400
612 612 c, t dbSNP:199796272
613 613 a, g dbSNP:769475203
616 616 a, g dbSNP:773120525
622 622 c, t dbSNP:762743351
641 641 a, g dbSNP:375329209
645 645 a, t dbSNP:147644451
649 649 a, g dbSNP:774245437
652 652 c, g dbSNP:759509229
670 670 c, t dbSNP:767009862
678 678 g, t dbSNP:777426077
698 698 a, c dbSNP:775037825
701 701 c, t dbSNP:760185283
702 702 a, g, t dbSNP:375043323
708 708 c, t dbSNP:757620452
713 713 c, t dbSNP:765562149
733 733 a, c, t dbSNP:141536152
734 734 a, g dbSNP:780227761
741 741 c, g dbSNP:747092422
747 747 c, t dbSNP:755113753
753 753 c, g dbSNP:781496033
756 756 a, g dbSNP:748363362
778 778 c, g dbSNP:367714205
790 790 a, g dbSNP:778746605
792 792 c, t dbSNP:745791563
800 800 c, t dbSNP:771949985
806 806 a, c dbSNP:779946817
819 819 g, t dbSNP:199562610
828 828 a, g dbSNP:370177908
840 840 c, t dbSNP:147048921
841 841 a, g dbSNP:151058527
858 858 c, t dbSNP:140971014
867 867 c, t dbSNP:150224277
868 868 a, g dbSNP:78640841
877 877 a, g dbSNP:766896121
894 894 c, t dbSNP:752070991
898 898 a, c, g dbSNP:201328365
904 904 a, c dbSNP:752732593
908 908 a, c, g dbSNP:375091484
933 933 c, g dbSNP:754110365
945 945 c, t dbSNP:758160320
989 989 g, t dbSNP:28938474
990 990 a, g dbSNP:368135922
1004 1004 a, t dbSNP:754907416
1005 1005 a, c, t dbSNP:370445458
1015 1015 c, t dbSNP:755652052
1018 1018 c, t dbSNP:138858259
1019 1019 a, g dbSNP:748931901
1026 1026 c, t dbSNP:34495182
1027 1027 a, c dbSNP:774711057
1028 1028 a, g dbSNP:746247653
1031 1031 a, t dbSNP:772580057
1033 1033 a, c, t dbSNP:775830551
1050 1050 c, t dbSNP:145889644
1051 1051 a, g dbSNP:572207593
1064 1064 c, g dbSNP:764158066
1065 1065 c, t dbSNP:372765864
1069 1069 a, g, t dbSNP:541224543
1074 1074 a, g dbSNP:778553600
1083 1083 c, t dbSNP:750033041
1086 1086 c, t dbSNP:564100262
1087 1087 -, c dbSNP:766300906
1090 1090 c, t dbSNP:73334576
1091 1091 c, g dbSNP:747409546
1092 1092 a, c, g, t dbSNP:769106353
1101 1101 a, g dbSNP:550334195
1102 1102 a, g dbSNP:773396940
1103 1103 c, t dbSNP:201395852
1108 1108 c, g dbSNP:193204039
1109 1109 c, t dbSNP:759150888
1113 1113 c, t dbSNP:775385092
1114 1114 a, c, g, t dbSNP:760566882
1121 1121 c, t dbSNP:761870318
1122 1122 c, t dbSNP:142327430
1128 1128 c, t dbSNP:749954407
1129 1129 a, g dbSNP:143895322
1142 1142 a, c dbSNP:766046321
1149 1149 a, g dbSNP:751264961
1161 1161 c, t dbSNP:148616062
1167 1167 c, t dbSNP:141188668
1168 1168 a, g dbSNP:748587793
1173 1173 a, g dbSNP:113899943
1178 1178 c, t dbSNP:202061937
1185 1185 c, t dbSNP:777644345
1186 1186 a, g dbSNP:371242130
1187 1187 c, t dbSNP:3744438
1188 1188 a, g dbSNP:201565504
1189 1189 a, c, g dbSNP:746131244
1192 1192 c, g dbSNP:551973242
1193 1193 c, t dbSNP:200387033
1194 1194 a, g dbSNP:765251401
1203 1203 a, g dbSNP:772552261
1209 1209 a, c dbSNP:199780097
1216 1216 c, t dbSNP:765917350
1219 1219 c, t dbSNP:751211966
1224 1224 a, c dbSNP:370805904
1231 1231 a, g dbSNP:150233337
1233 1233 c, t dbSNP:767809364
1251 1251 c, t dbSNP:577197197
1252 1252 a, t dbSNP:756535438
1265 1265 a, g dbSNP:778365568
1273 1273 a, g dbSNP:767227688
1277 1277 a, c dbSNP:139626296
1278 1278 a, c, t dbSNP:761077197
1279 1279 c, t dbSNP:754364356
1280 1280 a, g dbSNP:200962877
1288 1288 g, t dbSNP:779069209
1291 1291 c, t dbSNP:750564852
1294 1294 c, g dbSNP:145267704
1304 1304 c, g, t dbSNP:61739275
1305 1305 a, t dbSNP:747237133
1306 1306 a, t dbSNP:769630174
1310 1310 a, g dbSNP:777491054
1318 1318 g, t dbSNP:749162745
1319 1319 c, t dbSNP:147641113
1320 1320 c, t dbSNP:115169711
1328 1328 c, t dbSNP:149749116
1329 1329 a, g dbSNP:200518950
1331 1331 c, g, t dbSNP:775175284
1332 1332 c, g dbSNP:61739274
1344 1344 c, g dbSNP:754259211
1351 1351 c, t dbSNP:372115732
1352 1352 a, g dbSNP:765868170
1353 1353 c, t dbSNP:751019794
1354 1354 -, c dbSNP:754897911
1359 1359 a, c, g, t dbSNP:140385970
1360 1360 a, c, g dbSNP:199727670
1361 1361 a, c, t dbSNP:143267075
1369 1369 a, g dbSNP:770803206
1393 1393 c, t dbSNP:778799625
1394 1394 a, c dbSNP:536406541
1396 1396 c, t dbSNP:577394793
1404 1404 a, g dbSNP:774920212
1410 1410 a, c dbSNP:760308988
1413 1413 c, g, t dbSNP:768224294
1446 1446 c, t dbSNP:375128049
1447 1447 a, g dbSNP:765739860
1455 1455 c, t dbSNP:773853893
1456 1456 a, g dbSNP:146829316
1458 1458 a, g dbSNP:766428417
1461 1461 a, g dbSNP:751591708
1463 1463 c, g dbSNP:140662248
1466 1466 g, t dbSNP:145825881
1473 1473 c, t dbSNP:7218485
1474 1474 a, g dbSNP:757013870
1477 1477 c, t dbSNP:778744732
1484 1484 c, g dbSNP:745791793
1486 1486 c, t dbSNP:758280314
1490 1490 c, t dbSNP:779577386
1491 1491 a, g dbSNP:143027472
1494 1494 g, t dbSNP:768262582
1500 1500 c, t dbSNP:150277085
1509 1509 a, g dbSNP:138356325
1521 1521 a, g dbSNP:370684273
1524 1524 a, g dbSNP:370669286
1528 1528 a, g dbSNP:773729072
1529 1529 c, g dbSNP:763447195
1536 1536 c, t dbSNP:766821708
1540 1540 a, g dbSNP:774437880
1545 1545 a, g dbSNP:759508725
1547 1547 c, t dbSNP:767623150
1548 1548 a, g dbSNP:752909576
1550 1550 c, t dbSNP:756385029
1552 1552 a, g dbSNP:764952897
1563 1563 a, g dbSNP:373379452
1566 1566 c, t dbSNP:758307212
1576 1576 c, t dbSNP:149248181
1578 1578 c, t dbSNP:529644070
1581 1581 a, g dbSNP:754444689
1589 1589 c, t dbSNP:780910584
1590 1590 c, t dbSNP:199998292
1591 1591 a, g dbSNP:769578381
1595 1595 c, t dbSNP:773667933
1598 1598 a, c, t dbSNP:376613781
1608 1608 a, g dbSNP:566653409
1609 1609 c, t dbSNP:370445781
1610 1610 a, g dbSNP:373815870
1617 1617 c, g dbSNP:772123763
1626 1626 c, t dbSNP:200860672
1627 1627 a, g dbSNP:760819355
1633 1633 a, c dbSNP:764323057
1640 1640 a, g dbSNP:754139697
1642 1642 c, t dbSNP:532216691
1647 1647 a, g dbSNP:766266920
1655 1655 c, g dbSNP:751441900
1658 1658 a, g dbSNP:75619997
1663 1663 a, g dbSNP:754983531
1665 1665 g, t dbSNP:780786210
1668 1668 c, g dbSNP:752282577
1682 1682 c, t dbSNP:755776465
1695 1695 c, t dbSNP:2270150
1696 1696 c, t dbSNP:749007884
1697 1697 a, g dbSNP:139609877
1699 1699 a, g dbSNP:779390654
1702 1702 c, t dbSNP:746302650
1703 1703 a, g dbSNP:772572337
1709 1709 c, t dbSNP:776086286
1710 1710 c, t dbSNP:760722978
1713 1713 a, c, t dbSNP:143608018
1714 1714 a, g dbSNP:762155896
1720 1720 c, t dbSNP:766033021
1722 1722 c, t dbSNP:147183215
1723 1723 c, t dbSNP:751377938
1732 1732 c, t dbSNP:759509799
1734 1734 c, t dbSNP:767580732
1735 1735 a, g dbSNP:140527546
1743 1743 c, g, t dbSNP:755651888
1745 1745 a, g dbSNP:753417448
1750 1750 c, t dbSNP:756946831
1754 1754 a, g dbSNP:537917982
1761 1761 a, g dbSNP:117324438
1767 1767 c, g dbSNP:772474213
1769 1769 c, t dbSNP:777040245
1770 1770 a, g dbSNP:188952741
1774 1774 c, t dbSNP:181321459
1790 1790 a, g dbSNP:768608302
1800 1800 c, t dbSNP:776788981
1804 1804 a, t dbSNP:748255000
1812 1812 -, ctt dbSNP:781174408
1815 1815 -, ctc dbSNP:747908174
1825 1825 c, t dbSNP:200436565
1826 1826 a, g dbSNP:773964621
1830 1830 a, c dbSNP:151223901
1835 1835 c, t dbSNP:767458043
1841 1841 a, g dbSNP:28936696
1856 1856 a, t dbSNP:372012864
1859 1859 a, g dbSNP:760750802
1860 1860 a, g dbSNP:763453760
1863 1863 c, t dbSNP:753391567
1864 1864 g, t dbSNP:756819237
1869 1869 a, g dbSNP:762062413
1878 1878 c, t dbSNP:750116867
1881 1881 c, t dbSNP:545088929
1882 1882 a, g dbSNP:758758874
1891 1891 c, t dbSNP:780456576
1894 1894 c, g, t dbSNP:149961771
1895 1895 a, g dbSNP:781095471
1898 1898 c, t dbSNP:748286433
1904 1904 a, g dbSNP:769809385
1907 1907 a, g dbSNP:773533114
1908 1908 c, t dbSNP:368731674
1911 1911 c, t dbSNP:144878479
1912 1912 a, g dbSNP:185650741
1917 1917 c, t dbSNP:760621927
1918 1918 a, g dbSNP:544357700
1933 1933 c, t dbSNP:775955071
1942 1942 a, t dbSNP:560799982
1966 1966 a, g dbSNP:529742456
1975 1975 a, g dbSNP:539840563
1984 1984 -, gt, gtgtgtgt dbSNP:149977669
1986 1986 a, t dbSNP:79894176
2014 2014 -, tg dbSNP:71885970
2024 2024 -, gt, gtgtgtgt, ta dbSNP:60535903
2025 2025 a, g dbSNP:77924694
2030 2030 c, t dbSNP:149034024
2035 2035 a, g dbSNP:111840354
2099 2099 -, cac dbSNP:756840229
2157 2157 c, t dbSNP:138718626
2173 2173 c, t dbSNP:531385585
2174 2174 g, t dbSNP:548690089
2186 2186 a, g dbSNP:568457409
2200 2200 a, c dbSNP:141279587
2214 2214 a, g dbSNP:58804550
2216 2216 g, t dbSNP:571164199
2293 2293 c, t dbSNP:77786466
2295 2295 -, gaa dbSNP:771729163
2303 2303 c, t dbSNP:556881465
2305 2305 c, t dbSNP:576739539
2329 2329 g, t dbSNP:180857223
2333 2333 c, t dbSNP:753474884
2344 2344 a, t dbSNP:559990367
2388 2388 a, t dbSNP:554691569
2397 2397 a, g dbSNP:561282671
2399 2399 c, t dbSNP:528911904
2440 2440 a, g dbSNP:574411058
2449 2449 c, t dbSNP:540228848
2468 2468 a, t dbSNP:559868966
2517 2517 c, t dbSNP:542201906
2543 2543 c, t dbSNP:187653934
2588 2588 -, t dbSNP:372489230
2592 2592 -, g dbSNP:370185892
2593 2593 -, tt dbSNP:745609877
2594 2594 -, g dbSNP:111608426
2594 2594 g, t dbSNP:3744437
2595 2595 -, t dbSNP:3833137
2609 2609 a, c dbSNP:377432317
2646 2646 a, g dbSNP:59569849
2653 2653 a, c dbSNP:191996071
2659 2659 c, t dbSNP:183868550
2696 2696 a, g dbSNP:187454602
2697 2697 c, t dbSNP:541669280
2728 2728 a, t dbSNP:547791456
2730 2730 a, g dbSNP:3744436
2750 2750 a, g dbSNP:3744435
2871 2871 a, g dbSNP:550543497
2888 2888 a, g dbSNP:190368544
2891 2891 c, t dbSNP:370432592
2895 2895 a, g dbSNP:78974571
2902 2902 a, c dbSNP:574499830
2952 2952 a, c dbSNP:138499283
2980 2980 a, g dbSNP:141979564
3051 3051 a, g dbSNP:576457297
3114 3114 -, ataa dbSNP:5821329
3129 3129 -, aata dbSNP:3033496
3136 3136 a, t dbSNP:545389486
3144 3144 c, t dbSNP:563392407
3161 3161 g, t dbSNP:562482480
3316 3316 -, at dbSNP:374788278
3317 3317 -, atat dbSNP:71355185
3317 3317 -, at dbSNP:147094207
3320 3320 -, atat, t dbSNP:71150616
3342 3342 -, ta dbSNP:547983914
3343 3343 -, tatatata dbSNP:56105625
3344 3344 a, c dbSNP:201283016
3356 3356 -, ct dbSNP:113244672
3357 3357 a, t dbSNP:111558928

Target ORF information:

RefSeq Version XM_011525490
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525490

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGGTTTTT CCCAACCCAG ACTGGCCCCG GCCCCTTTTT ATCTCCTGGG TTGCACTTGG 
CTTGTTTACT TTGCGAGGTC CTGGGAGTCG AAGTCTAAGC CCGGGATGAG GAGGCCTCTG
AACAAGATGG GCGAGAGGCC TGAGCCCGGG GATGCTCCGG GAGGGCCCGG GGAGAGCGGG
AAGAAGGAGA TGCTGCAGGA TAAGGGCCTG TCCGAGAGCG AGGAGGCCTT CCGGGCCCCG
GGCCCAGCGC TCGGAGAGGC CAGCGCAGCC AACGCCCCCG AGCCCGCGCT GGCAGCGCCG
GGCCTCAGCG GAGCCGCGCT AGGCAGCCCC CCGGGACCCG GGGCCGACGT CGTCGCCGCC
GCCGCCGCGG AGCAGACCAT CGAGAACATC AAGGTGGGGC TGCATGAGAA GGAGCTCTGG
AAGAAGTTCC ACGAGGCGGG CACCGAGATG ATCATCACTA AGGCTGGCAG GAGGATGTTC
CCCAGCTACA AGGTAAAAGT CACAGGCATG AACCCCAAGA CCAAGTATAT CCTGCTGATT
GACATTGTCC CTGCCGATGA CCATCGCTAC AAGTTCTGTG ACAACAAATG GATGGTGGCA
GGGAAGGCTG AGCCAGCCAT GCCAGGAAGG CTGTATGTCC ACCCGGATTC TCCTGCCACA
GGAGCCCACT GGATGCGGCA GCTGGTCTCC TTCCAGAAGC TGAAGCTGAC AAACAACCAC
CTGGACCCCT TTGGCCATAT CATCCTCAAC TCTATGCACA AGTACCAGCC GCGGCTCCAC
ATCGTTAAGG CTGATGAGAA CAATGCTTTC GGCTCCAAAA ACACTGCTTT CTGCACCCAC
GTGTTCCCAG AGACCTCCTT CATCTCTGTG ACCTCCTACC AGAATCACAA GATCACCCAG
CTGAAAATTG AGAACAACCC TTTTGCCAAG GGATTCCGGG GCAGTGATGA CAGTGACCTG
CGTGTGGCCC GACTGCAGAG CAAAGAATAC CCCGTGATTT CCAAAAGCAT CATGAGGCAG
AGGCTCATCT CCCCCCAGCT CTCAGCCACA CCGGACGTGG GCCCCCTGCT CGGCACCCAC
CAGGCACTCC AGCACTACCA GCACGAGAAC GGGGCACACT CACAGCTCGC GGAGCCGCAG
GACCTGCCCC TCAGCACCTT TCCCACCCAG AGGGACTCAA GCCTCTTCTA TCACTGCCTG
AAAAGACGAG CAGACGGTAC CCGCCACCTG GACTTACCTT GCAAGCGATC CTATCTGGAA
GCCCCCTCTT CGGTGGGGGA GGATCACTAT TTCCGTTCCC CCCCTCCCTA CGACCAGCAA
ATGCTGAGCC CCTCCTACTG CAGTGAGGTG ACCCCCAGAG AAGCATGTAT GTACTCAGGT
TCAGGGCCCG AGATTGCCGG GGTGTCTGGG GTGGACGACC TGCCCCCACC TCCGCTGAGC
TGTAACATGT GGACTTCAGT GTCGCCGTAC ACCAGCTATA GCGTGCAGAC GATGGAGACT
GTGCCGTACC AGCCCTTCCC CACGCACTTC ACCGCCACCA CCATGATGCC GCGGCTGCCC
ACCCTCTCCG CTCAGAGCTC CCAGCCACCA GGAAATGCCC ACTTTAGTGT CTACAATCAG
CTCTCCCAGT CTCAGGTCCG AGAGCGGGGG CCCAGCGCCT CATTCCCAAG AGAGCGCGGC
CTCCCCCAAG GGTGTGAGAG GAAGCCACCC TCGCCACATC TAAATGCTGC CAATGAGTTT
CTCTACTCTC AAACCTTCTC CTTGTCCCGA GAATCTTCCT TACAGTACCA TTCAGGAATG
GGGACTGTGG AGAACTGGAC TGACGGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62874
Accession Version XM_011525491.1
Sequence Information ORF Nucleotide Sequence (Length: 1827bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGGTTTTT CCCAACCCAG ACTGGCCCCG GCCCCTTTTT ATCTCCTGGG TTGCACTTGG 
CTTGTTTACT TTGCGAGGTC CTGGGAGTCG AAGTCTAAGC CCGGGATGAG GAGGCCTCTG
AACAAGATGG GCGAGAGGCC TGAGCCCGGG GATGCTCCGG GAGGGCCCGG GGAGAGCGGG
AAGAAGGAGA TGCTGCAGGA TAAGGGCCTG TCCGAGAGCG AGGAGGCCTT CCGGGCCCCG
GGCCCAGCGC TCGGAGAGGC CAGCGCAGCC AACGCCCCCG AGCCCGCGCT GGCAGCGCCG
GGCCTCAGCG GAGCCGCGCT AGGCAGCCCC CCGGGACCCG GGGCCGACGT CGTCGCCGCC
GCCGCCGCGG AGCAGACCAT CGAGAACATC AAGGTGGGGC TGCATGAGAA GGAGCTCTGG
AAGAAGTTCC ACGAGGCGGG CACCGAGATG ATCATCACTA AGGCTGGCAG GAGGATGTTC
CCCAGCTACA AGGTAAAAGT CACAGGCATG AACCCCAAGA CCAAGTATAT CCTGCTGATT
GACATTGTCC CTGCCGATGA CCATCGCTAC AAGTTCTGTG ACAACAAATG GATGGTGGCA
GGGAAGGCTG AGCCAGCCAT GCCAGGAAGG CTGTATGTCC ACCCGGATTC TCCTGCCACA
GGAGCCCACT GGATGCGGCA GCTGGTCTCC TTCCAGAAGC TGAAGCTGAC AAACAACCAC
CTGGACCCCT TTGGCCATAT CATCCTCAAC TCTATGCACA AGTACCAGCC GCGGCTCCAC
ATCGTTAAGG CTGATGAGAA CAATGCTTTC GGCTCCAAAA ACACTGCTTT CTGCACCCAC
GTGTTCCCAG AGACCTCCTT CATCTCTGTG ACCTCCTACC AGAATCACAA GATCACCCAG
CTGAAAATTG AGAACAACCC TTTTGCCAAG GGATTCCGGG GCAGTGATGA CAGTGACCTG
CGTGTGGCCC GACTGCAGAG CAAAGAATAC CCCGTGATTT CCAAAAGCAT CATGAGGCAG
AGGCTCATCT CCCCCCAGCT CTCAGCCACA CCGGACGTGG GCCCCCTGCT CGGCACCCAC
CAGGCACTCC AGCACTACCA GCACGAGAAC GGGGCACACT CACAGCTCGC GGAGCCGCAG
GACCTGCCCC TCAGCACCTT TCCCACCCAG AGGGACTCAA GCCTCTTCTA TCACTGCCTG
AAAAGACGAG ACGGTACCCG CCACCTGGAC TTACCTTGCA AGCGATCCTA TCTGGAAGCC
CCCTCTTCGG TGGGGGAGGA TCACTATTTC CGTTCCCCCC CTCCCTACGA CCAGCAAATG
CTGAGCCCCT CCTACTGCAG TGAGGTGACC CCCAGAGAAG CATGTATGTA CTCAGGTTCA
GGGCCCGAGA TTGCCGGGGT GTCTGGGGTG GACGACCTGC CCCCACCTCC GCTGAGCTGT
AACATGTGGA CTTCAGTGTC GCCGTACACC AGCTATAGCG TGCAGACGAT GGAGACTGTG
CCGTACCAGC CCTTCCCCAC GCACTTCACC GCCACCACCA TGATGCCGCG GCTGCCCACC
CTCTCCGCTC AGAGCTCCCA GCCACCAGGA AATGCCCACT TTAGTGTCTA CAATCAGCTC
TCCCAGTCTC AGGTCCGAGA GCGGGGGCCC AGCGCCTCAT TCCCAAGAGA GCGCGGCCTC
CCCCAAGGGT GTGAGAGGAA GCCACCCTCG CCACATCTAA ATGCTGCCAA TGAGTTTCTC
TACTCTCAAA CCTTCTCCTT GTCCCGAGAA TCTTCCTTAC AGTACCATTC AGGAATGGGG
ACTGTGGAGA ACTGGACTGA CGGATGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011523793.1
CDS58..1884
Misc Feature(1)442..1011(+)
Misc Feature(2)508..999(+)
Misc Feature(3)580..849(+)
Translation
Position Chain Variation Link
45 45 a, c dbSNP:181750228
86 86 c, t dbSNP:114542698
103 103 c, t dbSNP:545336781
162 162 c, g dbSNP:555178103
173 173 c, t dbSNP:112225845
185 185 c, t dbSNP:775575406
190 190 a, g dbSNP:565702742
192 192 c, g dbSNP:535141245
204 204 c, t dbSNP:115284369
225 225 c, t dbSNP:760687344
228 228 g, t dbSNP:577978421
242 242 a, g dbSNP:543879893
259 259 -, a dbSNP:769433167
262 262 a, g dbSNP:117410176
263 263 c, g dbSNP:3744448
264 264 c, t dbSNP:752448330
269 269 c, t dbSNP:776660359
270 270 c, t dbSNP:777880490
271 271 c, g dbSNP:748755835
280 280 a, g dbSNP:756802394
287 287 c, t dbSNP:778604942
288 288 c, t dbSNP:745523181
289 289 c, t dbSNP:771639735
309 309 c, g dbSNP:775860775
310 310 c, g dbSNP:747369610
311 311 c, g dbSNP:769157857
317 317 c, t dbSNP:573485618
340 340 c, t dbSNP:762348865
349 349 g, t dbSNP:765299000
350 350 c, t dbSNP:148424252
392 392 g, t dbSNP:558901097
396 396 c, t dbSNP:763175971
400 400 a, g dbSNP:766513791
402 402 a, c dbSNP:752496226
405 405 c, t dbSNP:755880238
407 407 c, t dbSNP:764025450
411 411 -, cgc dbSNP:772891363
418 418 a, g dbSNP:575407008
421 421 a, g dbSNP:80164240
430 430 c, t dbSNP:104894648
436 436 a, t dbSNP:554741896
441 441 a, g dbSNP:759838845
453 453 c, g dbSNP:763974215
454 454 a, g dbSNP:753701583
455 455 a, g dbSNP:761779230
472 472 c, g dbSNP:765300595
480 480 g, t dbSNP:182844772
495 495 a, g dbSNP:35070263
501 501 a, c dbSNP:779655731
504 504 a, g dbSNP:751168165
509 509 c, t dbSNP:754685175
522 522 g, t dbSNP:3744447
552 552 a, g dbSNP:370004814
559 559 a, g dbSNP:753009856
562 562 a, g dbSNP:200429513
569 569 a, g dbSNP:778236633
573 573 c, t dbSNP:749751811
581 581 a, g dbSNP:377571192
589 589 c, t dbSNP:778794211
595 595 a, g dbSNP:745977352
599 599 a, g dbSNP:772372461
605 605 c, t dbSNP:200977400
612 612 c, t dbSNP:199796272
613 613 a, g dbSNP:769475203
616 616 a, g dbSNP:773120525
622 622 c, t dbSNP:762743351
641 641 a, g dbSNP:375329209
645 645 a, t dbSNP:147644451
649 649 a, g dbSNP:774245437
652 652 c, g dbSNP:759509229
670 670 c, t dbSNP:767009862
678 678 g, t dbSNP:777426077
698 698 a, c dbSNP:775037825
701 701 c, t dbSNP:760185283
702 702 a, g, t dbSNP:375043323
708 708 c, t dbSNP:757620452
713 713 c, t dbSNP:765562149
733 733 a, c, t dbSNP:141536152
734 734 a, g dbSNP:780227761
741 741 c, g dbSNP:747092422
747 747 c, t dbSNP:755113753
753 753 c, g dbSNP:781496033
756 756 a, g dbSNP:748363362
778 778 c, g dbSNP:367714205
790 790 a, g dbSNP:778746605
792 792 c, t dbSNP:745791563
800 800 c, t dbSNP:771949985
806 806 a, c dbSNP:779946817
819 819 g, t dbSNP:199562610
828 828 a, g dbSNP:370177908
840 840 c, t dbSNP:147048921
841 841 a, g dbSNP:151058527
858 858 c, t dbSNP:140971014
867 867 c, t dbSNP:150224277
868 868 a, g dbSNP:78640841
877 877 a, g dbSNP:766896121
894 894 c, t dbSNP:752070991
898 898 a, c, g dbSNP:201328365
904 904 a, c dbSNP:752732593
908 908 a, c, g dbSNP:375091484
933 933 c, g dbSNP:754110365
945 945 c, t dbSNP:758160320
989 989 g, t dbSNP:28938474
990 990 a, g dbSNP:368135922
1004 1004 a, t dbSNP:754907416
1005 1005 a, c, t dbSNP:370445458
1015 1015 c, t dbSNP:755652052
1018 1018 c, t dbSNP:138858259
1019 1019 a, g dbSNP:748931901
1026 1026 c, t dbSNP:34495182
1027 1027 a, c dbSNP:774711057
1028 1028 a, g dbSNP:746247653
1031 1031 a, t dbSNP:772580057
1033 1033 a, c, t dbSNP:775830551
1050 1050 c, t dbSNP:145889644
1051 1051 a, g dbSNP:572207593
1064 1064 c, g dbSNP:764158066
1065 1065 c, t dbSNP:372765864
1069 1069 a, g, t dbSNP:541224543
1074 1074 a, g dbSNP:778553600
1083 1083 c, t dbSNP:750033041
1086 1086 c, t dbSNP:564100262
1087 1087 -, c dbSNP:766300906
1090 1090 c, t dbSNP:73334576
1091 1091 c, g dbSNP:747409546
1092 1092 a, c, g, t dbSNP:769106353
1101 1101 a, g dbSNP:550334195
1102 1102 a, g dbSNP:773396940
1103 1103 c, t dbSNP:201395852
1108 1108 c, g dbSNP:193204039
1109 1109 c, t dbSNP:759150888
1113 1113 c, t dbSNP:775385092
1114 1114 a, c, g, t dbSNP:760566882
1121 1121 c, t dbSNP:761870318
1122 1122 c, t dbSNP:142327430
1128 1128 c, t dbSNP:749954407
1129 1129 a, g dbSNP:143895322
1142 1142 a, c dbSNP:766046321
1149 1149 a, g dbSNP:751264961
1161 1161 c, t dbSNP:148616062
1167 1167 c, t dbSNP:141188668
1168 1168 a, g dbSNP:748587793
1173 1173 a, g dbSNP:113899943
1178 1178 c, t dbSNP:202061937
1185 1185 c, t dbSNP:777644345
1186 1186 a, g dbSNP:371242130
1187 1187 c, t dbSNP:3744438
1188 1188 a, g dbSNP:201565504
1189 1189 a, c, g dbSNP:746131244
1192 1192 c, g dbSNP:551973242
1193 1193 c, t dbSNP:200387033
1194 1194 a, g dbSNP:765251401
1203 1203 a, g dbSNP:772552261
1209 1209 a, c dbSNP:199780097
1216 1216 c, t dbSNP:765917350
1219 1219 c, t dbSNP:751211966
1224 1224 a, c dbSNP:370805904
1231 1231 a, g dbSNP:150233337
1233 1233 c, t dbSNP:767809364
1251 1251 c, t dbSNP:577197197
1252 1252 a, t dbSNP:756535438
1265 1265 a, g dbSNP:778365568
1270 1270 a, g dbSNP:767227688
1274 1274 a, c dbSNP:139626296
1275 1275 a, c, t dbSNP:761077197
1276 1276 c, t dbSNP:754364356
1277 1277 a, g dbSNP:200962877
1285 1285 g, t dbSNP:779069209
1288 1288 c, t dbSNP:750564852
1291 1291 c, g dbSNP:145267704
1301 1301 c, g, t dbSNP:61739275
1302 1302 a, t dbSNP:747237133
1303 1303 a, t dbSNP:769630174
1307 1307 a, g dbSNP:777491054
1315 1315 g, t dbSNP:749162745
1316 1316 c, t dbSNP:147641113
1317 1317 c, t dbSNP:115169711
1325 1325 c, t dbSNP:149749116
1326 1326 a, g dbSNP:200518950
1328 1328 c, g, t dbSNP:775175284
1329 1329 c, g dbSNP:61739274
1341 1341 c, g dbSNP:754259211
1348 1348 c, t dbSNP:372115732
1349 1349 a, g dbSNP:765868170
1350 1350 c, t dbSNP:751019794
1351 1351 -, c dbSNP:754897911
1356 1356 a, c, g, t dbSNP:140385970
1357 1357 a, c, g dbSNP:199727670
1358 1358 a, c, t dbSNP:143267075
1366 1366 a, g dbSNP:770803206
1390 1390 c, t dbSNP:778799625
1391 1391 a, c dbSNP:536406541
1393 1393 c, t dbSNP:577394793
1401 1401 a, g dbSNP:774920212
1407 1407 a, c dbSNP:760308988
1410 1410 c, g, t dbSNP:768224294
1443 1443 c, t dbSNP:375128049
1444 1444 a, g dbSNP:765739860
1452 1452 c, t dbSNP:773853893
1453 1453 a, g dbSNP:146829316
1455 1455 a, g dbSNP:766428417
1458 1458 a, g dbSNP:751591708
1460 1460 c, g dbSNP:140662248
1463 1463 g, t dbSNP:145825881
1470 1470 c, t dbSNP:7218485
1471 1471 a, g dbSNP:757013870
1474 1474 c, t dbSNP:778744732
1481 1481 c, g dbSNP:745791793
1483 1483 c, t dbSNP:758280314
1487 1487 c, t dbSNP:779577386
1488 1488 a, g dbSNP:143027472
1491 1491 g, t dbSNP:768262582
1497 1497 c, t dbSNP:150277085
1506 1506 a, g dbSNP:138356325
1518 1518 a, g dbSNP:370684273
1521 1521 a, g dbSNP:370669286
1525 1525 a, g dbSNP:773729072
1526 1526 c, g dbSNP:763447195
1533 1533 c, t dbSNP:766821708
1537 1537 a, g dbSNP:774437880
1542 1542 a, g dbSNP:759508725
1544 1544 c, t dbSNP:767623150
1545 1545 a, g dbSNP:752909576
1547 1547 c, t dbSNP:756385029
1549 1549 a, g dbSNP:764952897
1560 1560 a, g dbSNP:373379452
1563 1563 c, t dbSNP:758307212
1573 1573 c, t dbSNP:149248181
1575 1575 c, t dbSNP:529644070
1578 1578 a, g dbSNP:754444689
1586 1586 c, t dbSNP:780910584
1587 1587 c, t dbSNP:199998292
1588 1588 a, g dbSNP:769578381
1592 1592 c, t dbSNP:773667933
1595 1595 a, c, t dbSNP:376613781
1605 1605 a, g dbSNP:566653409
1606 1606 c, t dbSNP:370445781
1607 1607 a, g dbSNP:373815870
1614 1614 c, g dbSNP:772123763
1623 1623 c, t dbSNP:200860672
1624 1624 a, g dbSNP:760819355
1630 1630 a, c dbSNP:764323057
1637 1637 a, g dbSNP:754139697
1639 1639 c, t dbSNP:532216691
1644 1644 a, g dbSNP:766266920
1652 1652 c, g dbSNP:751441900
1655 1655 a, g dbSNP:75619997
1660 1660 a, g dbSNP:754983531
1662 1662 g, t dbSNP:780786210
1665 1665 c, g dbSNP:752282577
1679 1679 c, t dbSNP:755776465
1692 1692 c, t dbSNP:2270150
1693 1693 c, t dbSNP:749007884
1694 1694 a, g dbSNP:139609877
1696 1696 a, g dbSNP:779390654
1699 1699 c, t dbSNP:746302650
1700 1700 a, g dbSNP:772572337
1706 1706 c, t dbSNP:776086286
1707 1707 c, t dbSNP:760722978
1710 1710 a, c, t dbSNP:143608018
1711 1711 a, g dbSNP:762155896
1717 1717 c, t dbSNP:766033021
1719 1719 c, t dbSNP:147183215
1720 1720 c, t dbSNP:751377938
1729 1729 c, t dbSNP:759509799
1731 1731 c, t dbSNP:767580732
1732 1732 a, g dbSNP:140527546
1740 1740 c, g, t dbSNP:755651888
1742 1742 a, g dbSNP:753417448
1747 1747 c, t dbSNP:756946831
1751 1751 a, g dbSNP:537917982
1758 1758 a, g dbSNP:117324438
1764 1764 c, g dbSNP:772474213
1766 1766 c, t dbSNP:777040245
1767 1767 a, g dbSNP:188952741
1771 1771 c, t dbSNP:181321459
1787 1787 a, g dbSNP:768608302
1797 1797 c, t dbSNP:776788981
1801 1801 a, t dbSNP:748255000
1809 1809 -, ctt dbSNP:781174408
1812 1812 -, ctc dbSNP:747908174
1822 1822 c, t dbSNP:200436565
1823 1823 a, g dbSNP:773964621
1827 1827 a, c dbSNP:151223901
1832 1832 c, t dbSNP:767458043
1838 1838 a, g dbSNP:28936696
1853 1853 a, t dbSNP:372012864
1856 1856 a, g dbSNP:760750802
1857 1857 a, g dbSNP:763453760
1860 1860 c, t dbSNP:753391567
1861 1861 g, t dbSNP:756819237
1866 1866 a, g dbSNP:762062413
1875 1875 c, t dbSNP:750116867
1878 1878 c, t dbSNP:545088929
1879 1879 a, g dbSNP:758758874
1888 1888 c, t dbSNP:780456576
1891 1891 c, g, t dbSNP:149961771
1892 1892 a, g dbSNP:781095471
1895 1895 c, t dbSNP:748286433
1901 1901 a, g dbSNP:769809385
1904 1904 a, g dbSNP:773533114
1905 1905 c, t dbSNP:368731674
1908 1908 c, t dbSNP:144878479
1909 1909 a, g dbSNP:185650741
1914 1914 c, t dbSNP:760621927
1915 1915 a, g dbSNP:544357700
1930 1930 c, t dbSNP:775955071
1939 1939 a, t dbSNP:560799982
1963 1963 a, g dbSNP:529742456
1972 1972 a, g dbSNP:539840563
1981 1981 -, gt, gtgtgtgt dbSNP:149977669
1983 1983 a, t dbSNP:79894176
2011 2011 -, tg dbSNP:71885970
2021 2021 -, gt, gtgtgtgt, ta dbSNP:60535903
2022 2022 a, g dbSNP:77924694
2027 2027 c, t dbSNP:149034024
2032 2032 a, g dbSNP:111840354
2096 2096 -, cac dbSNP:756840229
2154 2154 c, t dbSNP:138718626
2170 2170 c, t dbSNP:531385585
2171 2171 g, t dbSNP:548690089
2183 2183 a, g dbSNP:568457409
2197 2197 a, c dbSNP:141279587
2211 2211 a, g dbSNP:58804550
2213 2213 g, t dbSNP:571164199
2290 2290 c, t dbSNP:77786466
2292 2292 -, gaa dbSNP:771729163
2300 2300 c, t dbSNP:556881465
2302 2302 c, t dbSNP:576739539
2326 2326 g, t dbSNP:180857223
2330 2330 c, t dbSNP:753474884
2341 2341 a, t dbSNP:559990367
2385 2385 a, t dbSNP:554691569
2394 2394 a, g dbSNP:561282671
2396 2396 c, t dbSNP:528911904
2437 2437 a, g dbSNP:574411058
2446 2446 c, t dbSNP:540228848
2465 2465 a, t dbSNP:559868966
2514 2514 c, t dbSNP:542201906
2540 2540 c, t dbSNP:187653934
2585 2585 -, t dbSNP:372489230
2589 2589 -, g dbSNP:370185892
2590 2590 -, tt dbSNP:745609877
2591 2591 -, g dbSNP:111608426
2591 2591 g, t dbSNP:3744437
2592 2592 -, t dbSNP:3833137
2606 2606 a, c dbSNP:377432317
2643 2643 a, g dbSNP:59569849
2650 2650 a, c dbSNP:191996071
2656 2656 c, t dbSNP:183868550
2693 2693 a, g dbSNP:187454602
2694 2694 c, t dbSNP:541669280
2725 2725 a, t dbSNP:547791456
2727 2727 a, g dbSNP:3744436
2747 2747 a, g dbSNP:3744435
2868 2868 a, g dbSNP:550543497
2885 2885 a, g dbSNP:190368544
2888 2888 c, t dbSNP:370432592
2892 2892 a, g dbSNP:78974571
2899 2899 a, c dbSNP:574499830
2949 2949 a, c dbSNP:138499283
2977 2977 a, g dbSNP:141979564
3048 3048 a, g dbSNP:576457297
3111 3111 -, ataa dbSNP:5821329
3126 3126 -, aata dbSNP:3033496
3133 3133 a, t dbSNP:545389486
3141 3141 c, t dbSNP:563392407
3158 3158 g, t dbSNP:562482480
3313 3313 -, at dbSNP:374788278
3314 3314 -, atat dbSNP:71355185
3314 3314 -, at dbSNP:147094207
3317 3317 -, atat, t dbSNP:71150616
3339 3339 -, ta dbSNP:547983914
3340 3340 -, tatatata dbSNP:56105625
3341 3341 a, c dbSNP:201283016
3353 3353 -, ct dbSNP:113244672
3354 3354 a, t dbSNP:111558928

Target ORF information:

RefSeq Version XM_011525491
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525491

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGGTTTTT CCCAACCCAG ACTGGCCCCG GCCCCTTTTT ATCTCCTGGG TTGCACTTGG 
CTTGTTTACT TTGCGAGGTC CTGGGAGTCG AAGTCTAAGC CCGGGATGAG GAGGCCTCTG
AACAAGATGG GCGAGAGGCC TGAGCCCGGG GATGCTCCGG GAGGGCCCGG GGAGAGCGGG
AAGAAGGAGA TGCTGCAGGA TAAGGGCCTG TCCGAGAGCG AGGAGGCCTT CCGGGCCCCG
GGCCCAGCGC TCGGAGAGGC CAGCGCAGCC AACGCCCCCG AGCCCGCGCT GGCAGCGCCG
GGCCTCAGCG GAGCCGCGCT AGGCAGCCCC CCGGGACCCG GGGCCGACGT CGTCGCCGCC
GCCGCCGCGG AGCAGACCAT CGAGAACATC AAGGTGGGGC TGCATGAGAA GGAGCTCTGG
AAGAAGTTCC ACGAGGCGGG CACCGAGATG ATCATCACTA AGGCTGGCAG GAGGATGTTC
CCCAGCTACA AGGTAAAAGT CACAGGCATG AACCCCAAGA CCAAGTATAT CCTGCTGATT
GACATTGTCC CTGCCGATGA CCATCGCTAC AAGTTCTGTG ACAACAAATG GATGGTGGCA
GGGAAGGCTG AGCCAGCCAT GCCAGGAAGG CTGTATGTCC ACCCGGATTC TCCTGCCACA
GGAGCCCACT GGATGCGGCA GCTGGTCTCC TTCCAGAAGC TGAAGCTGAC AAACAACCAC
CTGGACCCCT TTGGCCATAT CATCCTCAAC TCTATGCACA AGTACCAGCC GCGGCTCCAC
ATCGTTAAGG CTGATGAGAA CAATGCTTTC GGCTCCAAAA ACACTGCTTT CTGCACCCAC
GTGTTCCCAG AGACCTCCTT CATCTCTGTG ACCTCCTACC AGAATCACAA GATCACCCAG
CTGAAAATTG AGAACAACCC TTTTGCCAAG GGATTCCGGG GCAGTGATGA CAGTGACCTG
CGTGTGGCCC GACTGCAGAG CAAAGAATAC CCCGTGATTT CCAAAAGCAT CATGAGGCAG
AGGCTCATCT CCCCCCAGCT CTCAGCCACA CCGGACGTGG GCCCCCTGCT CGGCACCCAC
CAGGCACTCC AGCACTACCA GCACGAGAAC GGGGCACACT CACAGCTCGC GGAGCCGCAG
GACCTGCCCC TCAGCACCTT TCCCACCCAG AGGGACTCAA GCCTCTTCTA TCACTGCCTG
AAAAGACGAG ACGGTACCCG CCACCTGGAC TTACCTTGCA AGCGATCCTA TCTGGAAGCC
CCCTCTTCGG TGGGGGAGGA TCACTATTTC CGTTCCCCCC CTCCCTACGA CCAGCAAATG
CTGAGCCCCT CCTACTGCAG TGAGGTGACC CCCAGAGAAG CATGTATGTA CTCAGGTTCA
GGGCCCGAGA TTGCCGGGGT GTCTGGGGTG GACGACCTGC CCCCACCTCC GCTGAGCTGT
AACATGTGGA CTTCAGTGTC GCCGTACACC AGCTATAGCG TGCAGACGAT GGAGACTGTG
CCGTACCAGC CCTTCCCCAC GCACTTCACC GCCACCACCA TGATGCCGCG GCTGCCCACC
CTCTCCGCTC AGAGCTCCCA GCCACCAGGA AATGCCCACT TTAGTGTCTA CAATCAGCTC
TCCCAGTCTC AGGTCCGAGA GCGGGGGCCC AGCGCCTCAT TCCCAAGAGA GCGCGGCCTC
CCCCAAGGGT GTGAGAGGAA GCCACCCTCG CCACATCTAA ATGCTGCCAA TGAGTTTCTC
TACTCTCAAA CCTTCTCCTT GTCCCGAGAA TCTTCCTTAC AGTACCATTC AGGAATGGGG
ACTGTGGAGA ACTGGACTGA CGGATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50822
Accession Version XM_005257835.3
Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578831672. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005257892.2
CDS19..1659
Misc Feature(1)214..783(+)
Misc Feature(2)280..771(+)
Misc Feature(3)352..621(+)
Translation
Position Chain Variation Link
6 6 a, g dbSNP:562518594
31 31 -, a dbSNP:769433167
34 34 a, g dbSNP:117410176
35 35 c, g dbSNP:3744448
36 36 c, t dbSNP:752448330
41 41 c, t dbSNP:776660359
42 42 c, t dbSNP:777880490
43 43 c, g dbSNP:748755835
52 52 a, g dbSNP:756802394
59 59 c, t dbSNP:778604942
60 60 c, t dbSNP:745523181
61 61 c, t dbSNP:771639735
81 81 c, g dbSNP:775860775
82 82 c, g dbSNP:747369610
83 83 c, g dbSNP:769157857
89 89 c, t dbSNP:573485618
112 112 c, t dbSNP:762348865
121 121 g, t dbSNP:765299000
122 122 c, t dbSNP:148424252
164 164 g, t dbSNP:558901097
168 168 c, t dbSNP:763175971
172 172 a, g dbSNP:766513791
174 174 a, c dbSNP:752496226
177 177 c, t dbSNP:755880238
179 179 c, t dbSNP:764025450
183 183 -, cgc dbSNP:772891363
190 190 a, g dbSNP:575407008
193 193 a, g dbSNP:80164240
202 202 c, t dbSNP:104894648
208 208 a, t dbSNP:554741896
213 213 a, g dbSNP:759838845
225 225 c, g dbSNP:763974215
226 226 a, g dbSNP:753701583
227 227 a, g dbSNP:761779230
244 244 c, g dbSNP:765300595
252 252 g, t dbSNP:182844772
267 267 a, g dbSNP:35070263
273 273 a, c dbSNP:779655731
276 276 a, g dbSNP:751168165
281 281 c, t dbSNP:754685175
294 294 g, t dbSNP:3744447
324 324 a, g dbSNP:370004814
331 331 a, g dbSNP:753009856
334 334 a, g dbSNP:200429513
341 341 a, g dbSNP:778236633
345 345 c, t dbSNP:749751811
353 353 a, g dbSNP:377571192
361 361 c, t dbSNP:778794211
367 367 a, g dbSNP:745977352
371 371 a, g dbSNP:772372461
377 377 c, t dbSNP:200977400
384 384 c, t dbSNP:199796272
385 385 a, g dbSNP:769475203
388 388 a, g dbSNP:773120525
394 394 c, t dbSNP:762743351
413 413 a, g dbSNP:375329209
417 417 a, t dbSNP:147644451
421 421 a, g dbSNP:774245437
424 424 c, g dbSNP:759509229
442 442 c, t dbSNP:767009862
450 450 g, t dbSNP:777426077
470 470 a, c dbSNP:775037825
473 473 c, t dbSNP:760185283
474 474 a, g, t dbSNP:375043323
480 480 c, t dbSNP:757620452
485 485 c, t dbSNP:765562149
505 505 a, c, t dbSNP:141536152
506 506 a, g dbSNP:780227761
513 513 c, g dbSNP:747092422
519 519 c, t dbSNP:755113753
525 525 c, g dbSNP:781496033
528 528 a, g dbSNP:748363362
550 550 c, g dbSNP:367714205
562 562 a, g dbSNP:778746605
564 564 c, t dbSNP:745791563
572 572 c, t dbSNP:771949985
578 578 a, c dbSNP:779946817
591 591 g, t dbSNP:199562610
600 600 a, g dbSNP:370177908
612 612 c, t dbSNP:147048921
613 613 a, g dbSNP:151058527
630 630 c, t dbSNP:140971014
639 639 c, t dbSNP:150224277
640 640 a, g dbSNP:78640841
649 649 a, g dbSNP:766896121
666 666 c, t dbSNP:752070991
670 670 a, c, g dbSNP:201328365
676 676 a, c dbSNP:752732593
680 680 a, c, g dbSNP:375091484
705 705 c, g dbSNP:754110365
717 717 c, t dbSNP:758160320
761 761 g, t dbSNP:28938474
762 762 a, g dbSNP:368135922
776 776 a, t dbSNP:754907416
777 777 a, c, t dbSNP:370445458
787 787 c, t dbSNP:755652052
790 790 c, t dbSNP:138858259
791 791 a, g dbSNP:748931901
798 798 c, t dbSNP:34495182
799 799 a, c dbSNP:774711057
800 800 a, g dbSNP:746247653
803 803 a, t dbSNP:772580057
805 805 a, c, t dbSNP:775830551
822 822 c, t dbSNP:145889644
823 823 a, g dbSNP:572207593
836 836 c, g dbSNP:764158066
837 837 c, t dbSNP:372765864
841 841 a, g, t dbSNP:541224543
846 846 a, g dbSNP:778553600
855 855 c, t dbSNP:750033041
858 858 c, t dbSNP:564100262
859 859 -, c dbSNP:766300906
862 862 c, t dbSNP:73334576
863 863 c, g dbSNP:747409546
864 864 a, c, g, t dbSNP:769106353
873 873 a, g dbSNP:550334195
874 874 a, g dbSNP:773396940
875 875 c, t dbSNP:201395852
880 880 c, g dbSNP:193204039
881 881 c, t dbSNP:759150888
885 885 c, t dbSNP:775385092
886 886 a, c, g, t dbSNP:760566882
893 893 c, t dbSNP:761870318
894 894 c, t dbSNP:142327430
900 900 c, t dbSNP:749954407
901 901 a, g dbSNP:143895322
914 914 a, c dbSNP:766046321
921 921 a, g dbSNP:751264961
933 933 c, t dbSNP:148616062
939 939 c, t dbSNP:141188668
940 940 a, g dbSNP:748587793
945 945 a, g dbSNP:113899943
950 950 c, t dbSNP:202061937
957 957 c, t dbSNP:777644345
958 958 a, g dbSNP:371242130
959 959 c, t dbSNP:3744438
960 960 a, g dbSNP:201565504
961 961 a, c, g dbSNP:746131244
964 964 c, g dbSNP:551973242
965 965 c, t dbSNP:200387033
966 966 a, g dbSNP:765251401
975 975 a, g dbSNP:772552261
981 981 a, c dbSNP:199780097
988 988 c, t dbSNP:765917350
991 991 c, t dbSNP:751211966
996 996 a, c dbSNP:370805904
1003 1003 a, g dbSNP:150233337
1005 1005 c, t dbSNP:767809364
1023 1023 c, t dbSNP:577197197
1024 1024 a, t dbSNP:756535438
1037 1037 a, g dbSNP:778365568
1045 1045 a, g dbSNP:767227688
1049 1049 a, c dbSNP:139626296
1050 1050 a, c, t dbSNP:761077197
1051 1051 c, t dbSNP:754364356
1052 1052 a, g dbSNP:200962877
1060 1060 g, t dbSNP:779069209
1063 1063 c, t dbSNP:750564852
1066 1066 c, g dbSNP:145267704
1076 1076 c, g, t dbSNP:61739275
1077 1077 a, t dbSNP:747237133
1078 1078 a, t dbSNP:769630174
1082 1082 a, g dbSNP:777491054
1090 1090 g, t dbSNP:749162745
1091 1091 c, t dbSNP:147641113
1092 1092 c, t dbSNP:115169711
1100 1100 c, t dbSNP:149749116
1101 1101 a, g dbSNP:200518950
1103 1103 c, g, t dbSNP:775175284
1104 1104 c, g dbSNP:61739274
1116 1116 c, g dbSNP:754259211
1123 1123 c, t dbSNP:372115732
1124 1124 a, g dbSNP:765868170
1125 1125 c, t dbSNP:751019794
1126 1126 -, c dbSNP:754897911
1131 1131 a, c, g, t dbSNP:140385970
1132 1132 a, c, g dbSNP:199727670
1133 1133 a, c, t dbSNP:143267075
1141 1141 a, g dbSNP:770803206
1165 1165 c, t dbSNP:778799625
1166 1166 a, c dbSNP:536406541
1168 1168 c, t dbSNP:577394793
1176 1176 a, g dbSNP:774920212
1182 1182 a, c dbSNP:760308988
1185 1185 c, g, t dbSNP:768224294
1218 1218 c, t dbSNP:375128049
1219 1219 a, g dbSNP:765739860
1227 1227 c, t dbSNP:773853893
1228 1228 a, g dbSNP:146829316
1230 1230 a, g dbSNP:766428417
1233 1233 a, g dbSNP:751591708
1235 1235 c, g dbSNP:140662248
1238 1238 g, t dbSNP:145825881
1245 1245 c, t dbSNP:7218485
1246 1246 a, g dbSNP:757013870
1249 1249 c, t dbSNP:778744732
1256 1256 c, g dbSNP:745791793
1258 1258 c, t dbSNP:758280314
1262 1262 c, t dbSNP:779577386
1263 1263 a, g dbSNP:143027472
1266 1266 g, t dbSNP:768262582
1272 1272 c, t dbSNP:150277085
1281 1281 a, g dbSNP:138356325
1293 1293 a, g dbSNP:370684273
1296 1296 a, g dbSNP:370669286
1300 1300 a, g dbSNP:773729072
1301 1301 c, g dbSNP:763447195
1308 1308 c, t dbSNP:766821708
1312 1312 a, g dbSNP:774437880
1317 1317 a, g dbSNP:759508725
1319 1319 c, t dbSNP:767623150
1320 1320 a, g dbSNP:752909576
1322 1322 c, t dbSNP:756385029
1324 1324 a, g dbSNP:764952897
1335 1335 a, g dbSNP:373379452
1338 1338 c, t dbSNP:758307212
1348 1348 c, t dbSNP:149248181
1350 1350 c, t dbSNP:529644070
1353 1353 a, g dbSNP:754444689
1361 1361 c, t dbSNP:780910584
1362 1362 c, t dbSNP:199998292
1363 1363 a, g dbSNP:769578381
1367 1367 c, t dbSNP:773667933
1370 1370 a, c, t dbSNP:376613781
1380 1380 a, g dbSNP:566653409
1381 1381 c, t dbSNP:370445781
1382 1382 a, g dbSNP:373815870
1389 1389 c, g dbSNP:772123763
1398 1398 c, t dbSNP:200860672
1399 1399 a, g dbSNP:760819355
1405 1405 a, c dbSNP:764323057
1412 1412 a, g dbSNP:754139697
1414 1414 c, t dbSNP:532216691
1419 1419 a, g dbSNP:766266920
1427 1427 c, g dbSNP:751441900
1430 1430 a, g dbSNP:75619997
1435 1435 a, g dbSNP:754983531
1437 1437 g, t dbSNP:780786210
1440 1440 c, g dbSNP:752282577
1454 1454 c, t dbSNP:755776465
1467 1467 c, t dbSNP:2270150
1468 1468 c, t dbSNP:749007884
1469 1469 a, g dbSNP:139609877
1471 1471 a, g dbSNP:779390654
1474 1474 c, t dbSNP:746302650
1475 1475 a, g dbSNP:772572337
1481 1481 c, t dbSNP:776086286
1482 1482 c, t dbSNP:760722978
1485 1485 a, c, t dbSNP:143608018
1486 1486 a, g dbSNP:762155896
1492 1492 c, t dbSNP:766033021
1494 1494 c, t dbSNP:147183215
1495 1495 c, t dbSNP:751377938
1504 1504 c, t dbSNP:759509799
1506 1506 c, t dbSNP:767580732
1507 1507 a, g dbSNP:140527546
1515 1515 c, g, t dbSNP:755651888
1517 1517 a, g dbSNP:753417448
1522 1522 c, t dbSNP:756946831
1526 1526 a, g dbSNP:537917982
1533 1533 a, g dbSNP:117324438
1539 1539 c, g dbSNP:772474213
1541 1541 c, t dbSNP:777040245
1542 1542 a, g dbSNP:188952741
1546 1546 c, t dbSNP:181321459
1562 1562 a, g dbSNP:768608302
1572 1572 c, t dbSNP:776788981
1576 1576 a, t dbSNP:748255000
1584 1584 -, ctt dbSNP:781174408
1587 1587 -, ctc dbSNP:747908174
1597 1597 c, t dbSNP:200436565
1598 1598 a, g dbSNP:773964621
1602 1602 a, c dbSNP:151223901
1607 1607 c, t dbSNP:767458043
1613 1613 a, g dbSNP:28936696
1628 1628 a, t dbSNP:372012864
1631 1631 a, g dbSNP:760750802
1632 1632 a, g dbSNP:763453760
1635 1635 c, t dbSNP:753391567
1636 1636 g, t dbSNP:756819237
1641 1641 a, g dbSNP:762062413
1650 1650 c, t dbSNP:750116867
1653 1653 c, t dbSNP:545088929
1654 1654 a, g dbSNP:758758874
1663 1663 c, t dbSNP:780456576
1666 1666 c, g, t dbSNP:149961771
1667 1667 a, g dbSNP:781095471
1670 1670 c, t dbSNP:748286433
1676 1676 a, g dbSNP:769809385
1679 1679 a, g dbSNP:773533114
1680 1680 c, t dbSNP:368731674
1683 1683 c, t dbSNP:144878479
1684 1684 a, g dbSNP:185650741
1689 1689 c, t dbSNP:760621927
1690 1690 a, g dbSNP:544357700
1705 1705 c, t dbSNP:775955071
1714 1714 a, t dbSNP:560799982
1738 1738 a, g dbSNP:529742456
1747 1747 a, g dbSNP:539840563
1756 1756 -, gt, gtgtgtgt dbSNP:149977669
1758 1758 a, t dbSNP:79894176
1786 1786 -, tg dbSNP:71885970
1796 1796 -, gt, gtgtgtgt, ta dbSNP:60535903
1797 1797 a, g dbSNP:77924694
1802 1802 c, t dbSNP:149034024
1807 1807 a, g dbSNP:111840354
1871 1871 -, cac dbSNP:756840229
1929 1929 c, t dbSNP:138718626
1945 1945 c, t dbSNP:531385585
1946 1946 g, t dbSNP:548690089
1958 1958 a, g dbSNP:568457409
1972 1972 a, c dbSNP:141279587
1986 1986 a, g dbSNP:58804550
1988 1988 g, t dbSNP:571164199
2065 2065 c, t dbSNP:77786466
2067 2067 -, gaa dbSNP:771729163
2075 2075 c, t dbSNP:556881465
2077 2077 c, t dbSNP:576739539
2101 2101 g, t dbSNP:180857223
2105 2105 c, t dbSNP:753474884
2116 2116 a, t dbSNP:559990367
2160 2160 a, t dbSNP:554691569
2169 2169 a, g dbSNP:561282671
2171 2171 c, t dbSNP:528911904
2212 2212 a, g dbSNP:574411058
2221 2221 c, t dbSNP:540228848
2240 2240 a, t dbSNP:559868966
2289 2289 c, t dbSNP:542201906
2315 2315 c, t dbSNP:187653934
2360 2360 -, t dbSNP:372489230
2364 2364 -, g dbSNP:370185892
2365 2365 -, tt dbSNP:745609877
2366 2366 -, g dbSNP:111608426
2366 2366 g, t dbSNP:3744437
2367 2367 -, t dbSNP:3833137
2381 2381 a, c dbSNP:377432317
2418 2418 a, g dbSNP:59569849
2425 2425 a, c dbSNP:191996071
2431 2431 c, t dbSNP:183868550
2468 2468 a, g dbSNP:187454602
2469 2469 c, t dbSNP:541669280
2500 2500 a, t dbSNP:547791456
2502 2502 a, g dbSNP:3744436
2522 2522 a, g dbSNP:3744435
2643 2643 a, g dbSNP:550543497
2660 2660 a, g dbSNP:190368544
2663 2663 c, t dbSNP:370432592
2667 2667 a, g dbSNP:78974571
2674 2674 a, c dbSNP:574499830
2724 2724 a, c dbSNP:138499283
2752 2752 a, g dbSNP:141979564
2823 2823 a, g dbSNP:576457297
2886 2886 -, ataa dbSNP:5821329
2901 2901 -, aata dbSNP:3033496
2908 2908 a, t dbSNP:545389486
2916 2916 c, t dbSNP:563392407
2933 2933 g, t dbSNP:562482480
3088 3088 -, at dbSNP:374788278
3089 3089 -, atat dbSNP:71355185
3089 3089 -, at dbSNP:147094207
3092 3092 -, atat, t dbSNP:71150616
3114 3114 -, ta dbSNP:547983914
3115 3115 -, tatatata dbSNP:56105625
3116 3116 a, c dbSNP:201283016
3128 3128 -, ct dbSNP:113244672
3129 3129 a, t dbSNP:111558928

Target ORF information:

RefSeq Version XM_005257835
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257835

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50822
Accession Version XM_005257837.2
Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530413201. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005257894.1
CDS165..1805
Misc Feature(1)360..929(+)
Misc Feature(2)426..917(+)
Misc Feature(3)498..767(+)
Translation
Position Chain Variation Link
7 7 a, g dbSNP:527993785
43 43 c, t dbSNP:548041261
74 74 g, t dbSNP:756830865
86 86 a, g dbSNP:778480137
105 105 c, t dbSNP:746419411
110 110 c, g dbSNP:772600502
132 132 a, g dbSNP:570958389
133 133 g, t dbSNP:539980918
134 134 c, t dbSNP:556472039
135 135 c, g dbSNP:749396096
136 136 c, t dbSNP:769099825
145 145 a, g dbSNP:138798857
156 156 a, g dbSNP:537918778
177 177 -, a dbSNP:769433167
180 180 a, g dbSNP:117410176
181 181 c, g dbSNP:3744448
182 182 c, t dbSNP:752448330
187 187 c, t dbSNP:776660359
188 188 c, t dbSNP:777880490
189 189 c, g dbSNP:748755835
198 198 a, g dbSNP:756802394
205 205 c, t dbSNP:778604942
206 206 c, t dbSNP:745523181
207 207 c, t dbSNP:771639735
227 227 c, g dbSNP:775860775
228 228 c, g dbSNP:747369610
229 229 c, g dbSNP:769157857
235 235 c, t dbSNP:573485618
258 258 c, t dbSNP:762348865
267 267 g, t dbSNP:765299000
268 268 c, t dbSNP:148424252
310 310 g, t dbSNP:558901097
314 314 c, t dbSNP:763175971
318 318 a, g dbSNP:766513791
320 320 a, c dbSNP:752496226
323 323 c, t dbSNP:755880238
325 325 c, t dbSNP:764025450
329 329 -, cgc dbSNP:772891363
336 336 a, g dbSNP:575407008
339 339 a, g dbSNP:80164240
348 348 c, t dbSNP:104894648
354 354 a, t dbSNP:554741896
359 359 a, g dbSNP:759838845
371 371 c, g dbSNP:763974215
372 372 a, g dbSNP:753701583
373 373 a, g dbSNP:761779230
390 390 c, g dbSNP:765300595
398 398 g, t dbSNP:182844772
413 413 a, g dbSNP:35070263
419 419 a, c dbSNP:779655731
422 422 a, g dbSNP:751168165
427 427 c, t dbSNP:754685175
440 440 g, t dbSNP:3744447
470 470 a, g dbSNP:370004814
477 477 a, g dbSNP:753009856
480 480 a, g dbSNP:200429513
487 487 a, g dbSNP:778236633
491 491 c, t dbSNP:749751811
499 499 a, g dbSNP:377571192
507 507 c, t dbSNP:778794211
513 513 a, g dbSNP:745977352
517 517 a, g dbSNP:772372461
523 523 c, t dbSNP:200977400
530 530 c, t dbSNP:199796272
531 531 a, g dbSNP:769475203
534 534 a, g dbSNP:773120525
540 540 c, t dbSNP:762743351
559 559 a, g dbSNP:375329209
563 563 a, t dbSNP:147644451
567 567 a, g dbSNP:774245437
570 570 c, g dbSNP:759509229
588 588 c, t dbSNP:767009862
596 596 g, t dbSNP:777426077
616 616 a, c dbSNP:775037825
619 619 c, t dbSNP:760185283
620 620 a, g, t dbSNP:375043323
626 626 c, t dbSNP:757620452
631 631 c, t dbSNP:765562149
651 651 a, c, t dbSNP:141536152
652 652 a, g dbSNP:780227761
659 659 c, g dbSNP:747092422
665 665 c, t dbSNP:755113753
671 671 c, g dbSNP:781496033
674 674 a, g dbSNP:748363362
696 696 c, g dbSNP:367714205
708 708 a, g dbSNP:778746605
710 710 c, t dbSNP:745791563
718 718 c, t dbSNP:771949985
724 724 a, c dbSNP:779946817
737 737 g, t dbSNP:199562610
746 746 a, g dbSNP:370177908
758 758 c, t dbSNP:147048921
759 759 a, g dbSNP:151058527
776 776 c, t dbSNP:140971014
785 785 c, t dbSNP:150224277
786 786 a, g dbSNP:78640841
795 795 a, g dbSNP:766896121
812 812 c, t dbSNP:752070991
816 816 a, c, g dbSNP:201328365
822 822 a, c dbSNP:752732593
826 826 a, c, g dbSNP:375091484
851 851 c, g dbSNP:754110365
863 863 c, t dbSNP:758160320
907 907 g, t dbSNP:28938474
908 908 a, g dbSNP:368135922
922 922 a, t dbSNP:754907416
923 923 a, c, t dbSNP:370445458
933 933 c, t dbSNP:755652052
936 936 c, t dbSNP:138858259
937 937 a, g dbSNP:748931901
944 944 c, t dbSNP:34495182
945 945 a, c dbSNP:774711057
946 946 a, g dbSNP:746247653
949 949 a, t dbSNP:772580057
951 951 a, c, t dbSNP:775830551
968 968 c, t dbSNP:145889644
969 969 a, g dbSNP:572207593
982 982 c, g dbSNP:764158066
983 983 c, t dbSNP:372765864
987 987 a, g, t dbSNP:541224543
992 992 a, g dbSNP:778553600
1001 1001 c, t dbSNP:750033041
1004 1004 c, t dbSNP:564100262
1005 1005 -, c dbSNP:766300906
1008 1008 c, t dbSNP:73334576
1009 1009 c, g dbSNP:747409546
1010 1010 a, c, g, t dbSNP:769106353
1019 1019 a, g dbSNP:550334195
1020 1020 a, g dbSNP:773396940
1021 1021 c, t dbSNP:201395852
1026 1026 c, g dbSNP:193204039
1027 1027 c, t dbSNP:759150888
1031 1031 c, t dbSNP:775385092
1032 1032 a, c, g, t dbSNP:760566882
1039 1039 c, t dbSNP:761870318
1040 1040 c, t dbSNP:142327430
1046 1046 c, t dbSNP:749954407
1047 1047 a, g dbSNP:143895322
1060 1060 a, c dbSNP:766046321
1067 1067 a, g dbSNP:751264961
1079 1079 c, t dbSNP:148616062
1085 1085 c, t dbSNP:141188668
1086 1086 a, g dbSNP:748587793
1091 1091 a, g dbSNP:113899943
1096 1096 c, t dbSNP:202061937
1103 1103 c, t dbSNP:777644345
1104 1104 a, g dbSNP:371242130
1105 1105 c, t dbSNP:3744438
1106 1106 a, g dbSNP:201565504
1107 1107 a, c, g dbSNP:746131244
1110 1110 c, g dbSNP:551973242
1111 1111 c, t dbSNP:200387033
1112 1112 a, g dbSNP:765251401
1121 1121 a, g dbSNP:772552261
1127 1127 a, c dbSNP:199780097
1134 1134 c, t dbSNP:765917350
1137 1137 c, t dbSNP:751211966
1142 1142 a, c dbSNP:370805904
1149 1149 a, g dbSNP:150233337
1151 1151 c, t dbSNP:767809364
1169 1169 c, t dbSNP:577197197
1170 1170 a, t dbSNP:756535438
1183 1183 a, g dbSNP:778365568
1191 1191 a, g dbSNP:767227688
1195 1195 a, c dbSNP:139626296
1196 1196 a, c, t dbSNP:761077197
1197 1197 c, t dbSNP:754364356
1198 1198 a, g dbSNP:200962877
1206 1206 g, t dbSNP:779069209
1209 1209 c, t dbSNP:750564852
1212 1212 c, g dbSNP:145267704
1222 1222 c, g, t dbSNP:61739275
1223 1223 a, t dbSNP:747237133
1224 1224 a, t dbSNP:769630174
1228 1228 a, g dbSNP:777491054
1236 1236 g, t dbSNP:749162745
1237 1237 c, t dbSNP:147641113
1238 1238 c, t dbSNP:115169711
1246 1246 c, t dbSNP:149749116
1247 1247 a, g dbSNP:200518950
1249 1249 c, g, t dbSNP:775175284
1250 1250 c, g dbSNP:61739274
1262 1262 c, g dbSNP:754259211
1269 1269 c, t dbSNP:372115732
1270 1270 a, g dbSNP:765868170
1271 1271 c, t dbSNP:751019794
1272 1272 -, c dbSNP:754897911
1277 1277 a, c, g, t dbSNP:140385970
1278 1278 a, c, g dbSNP:199727670
1279 1279 a, c, t dbSNP:143267075
1287 1287 a, g dbSNP:770803206
1311 1311 c, t dbSNP:778799625
1312 1312 a, c dbSNP:536406541
1314 1314 c, t dbSNP:577394793
1322 1322 a, g dbSNP:774920212
1328 1328 a, c dbSNP:760308988
1331 1331 c, g, t dbSNP:768224294
1364 1364 c, t dbSNP:375128049
1365 1365 a, g dbSNP:765739860
1373 1373 c, t dbSNP:773853893
1374 1374 a, g dbSNP:146829316
1376 1376 a, g dbSNP:766428417
1379 1379 a, g dbSNP:751591708
1381 1381 c, g dbSNP:140662248
1384 1384 g, t dbSNP:145825881
1391 1391 c, t dbSNP:7218485
1392 1392 a, g dbSNP:757013870
1395 1395 c, t dbSNP:778744732
1402 1402 c, g dbSNP:745791793
1404 1404 c, t dbSNP:758280314
1408 1408 c, t dbSNP:779577386
1409 1409 a, g dbSNP:143027472
1412 1412 g, t dbSNP:768262582
1418 1418 c, t dbSNP:150277085
1427 1427 a, g dbSNP:138356325
1439 1439 a, g dbSNP:370684273
1442 1442 a, g dbSNP:370669286
1446 1446 a, g dbSNP:773729072
1447 1447 c, g dbSNP:763447195
1454 1454 c, t dbSNP:766821708
1458 1458 a, g dbSNP:774437880
1463 1463 a, g dbSNP:759508725
1465 1465 c, t dbSNP:767623150
1466 1466 a, g dbSNP:752909576
1468 1468 c, t dbSNP:756385029
1470 1470 a, g dbSNP:764952897
1481 1481 a, g dbSNP:373379452
1484 1484 c, t dbSNP:758307212
1494 1494 c, t dbSNP:149248181
1496 1496 c, t dbSNP:529644070
1499 1499 a, g dbSNP:754444689
1507 1507 c, t dbSNP:780910584
1508 1508 c, t dbSNP:199998292
1509 1509 a, g dbSNP:769578381
1513 1513 c, t dbSNP:773667933
1516 1516 a, c, t dbSNP:376613781
1526 1526 a, g dbSNP:566653409
1527 1527 c, t dbSNP:370445781
1528 1528 a, g dbSNP:373815870
1535 1535 c, g dbSNP:772123763
1544 1544 c, t dbSNP:200860672
1545 1545 a, g dbSNP:760819355
1551 1551 a, c dbSNP:764323057
1558 1558 a, g dbSNP:754139697
1560 1560 c, t dbSNP:532216691
1565 1565 a, g dbSNP:766266920
1573 1573 c, g dbSNP:751441900
1576 1576 a, g dbSNP:75619997
1581 1581 a, g dbSNP:754983531
1583 1583 g, t dbSNP:780786210
1586 1586 c, g dbSNP:752282577
1600 1600 c, t dbSNP:755776465
1613 1613 c, t dbSNP:2270150
1614 1614 c, t dbSNP:749007884
1615 1615 a, g dbSNP:139609877
1617 1617 a, g dbSNP:779390654
1620 1620 c, t dbSNP:746302650
1621 1621 a, g dbSNP:772572337
1627 1627 c, t dbSNP:776086286
1628 1628 c, t dbSNP:760722978
1631 1631 a, c, t dbSNP:143608018
1632 1632 a, g dbSNP:762155896
1638 1638 c, t dbSNP:766033021
1640 1640 c, t dbSNP:147183215
1641 1641 c, t dbSNP:751377938
1650 1650 c, t dbSNP:759509799
1652 1652 c, t dbSNP:767580732
1653 1653 a, g dbSNP:140527546
1661 1661 c, g, t dbSNP:755651888
1663 1663 a, g dbSNP:753417448
1668 1668 c, t dbSNP:756946831
1672 1672 a, g dbSNP:537917982
1679 1679 a, g dbSNP:117324438
1685 1685 c, g dbSNP:772474213
1687 1687 c, t dbSNP:777040245
1688 1688 a, g dbSNP:188952741
1692 1692 c, t dbSNP:181321459
1708 1708 a, g dbSNP:768608302
1718 1718 c, t dbSNP:776788981
1722 1722 a, t dbSNP:748255000
1730 1730 -, ctt dbSNP:781174408
1733 1733 -, ctc dbSNP:747908174
1743 1743 c, t dbSNP:200436565
1744 1744 a, g dbSNP:773964621
1748 1748 a, c dbSNP:151223901
1753 1753 c, t dbSNP:767458043
1759 1759 a, g dbSNP:28936696
1774 1774 a, t dbSNP:372012864
1777 1777 a, g dbSNP:760750802
1778 1778 a, g dbSNP:763453760
1781 1781 c, t dbSNP:753391567
1782 1782 g, t dbSNP:756819237
1787 1787 a, g dbSNP:762062413
1796 1796 c, t dbSNP:750116867
1799 1799 c, t dbSNP:545088929
1800 1800 a, g dbSNP:758758874
1809 1809 c, t dbSNP:780456576
1812 1812 c, g, t dbSNP:149961771
1813 1813 a, g dbSNP:781095471
1816 1816 c, t dbSNP:748286433
1822 1822 a, g dbSNP:769809385
1825 1825 a, g dbSNP:773533114
1826 1826 c, t dbSNP:368731674
1829 1829 c, t dbSNP:144878479
1830 1830 a, g dbSNP:185650741
1835 1835 c, t dbSNP:760621927
1836 1836 a, g dbSNP:544357700
1851 1851 c, t dbSNP:775955071
1860 1860 a, t dbSNP:560799982
1884 1884 a, g dbSNP:529742456
1893 1893 a, g dbSNP:539840563
1902 1902 -, gt, gtgtgtgt dbSNP:149977669
1904 1904 a, t dbSNP:79894176
1932 1932 -, tg dbSNP:71885970
1942 1942 -, gt, gtgtgtgt, ta dbSNP:60535903
1943 1943 a, g dbSNP:77924694
1948 1948 c, t dbSNP:149034024
1953 1953 a, g dbSNP:111840354
2017 2017 -, cac dbSNP:756840229
2075 2075 c, t dbSNP:138718626
2091 2091 c, t dbSNP:531385585
2092 2092 g, t dbSNP:548690089
2104 2104 a, g dbSNP:568457409
2118 2118 a, c dbSNP:141279587
2132 2132 a, g dbSNP:58804550
2134 2134 g, t dbSNP:571164199
2211 2211 c, t dbSNP:77786466
2213 2213 -, gaa dbSNP:771729163
2221 2221 c, t dbSNP:556881465
2223 2223 c, t dbSNP:576739539
2247 2247 g, t dbSNP:180857223
2251 2251 c, t dbSNP:753474884
2262 2262 a, t dbSNP:559990367
2306 2306 a, t dbSNP:554691569
2315 2315 a, g dbSNP:561282671
2317 2317 c, t dbSNP:528911904
2358 2358 a, g dbSNP:574411058
2367 2367 c, t dbSNP:540228848
2386 2386 a, t dbSNP:559868966
2435 2435 c, t dbSNP:542201906
2461 2461 c, t dbSNP:187653934
2506 2506 -, t dbSNP:372489230
2510 2510 -, g dbSNP:370185892
2511 2511 -, tt dbSNP:745609877
2512 2512 -, g dbSNP:111608426
2512 2512 g, t dbSNP:3744437
2513 2513 -, t dbSNP:3833137
2527 2527 a, c dbSNP:377432317
2564 2564 a, g dbSNP:59569849
2571 2571 a, c dbSNP:191996071
2577 2577 c, t dbSNP:183868550
2614 2614 a, g dbSNP:187454602
2615 2615 c, t dbSNP:541669280
2646 2646 a, t dbSNP:547791456
2648 2648 a, g dbSNP:3744436
2668 2668 a, g dbSNP:3744435
2789 2789 a, g dbSNP:550543497
2806 2806 a, g dbSNP:190368544
2809 2809 c, t dbSNP:370432592
2813 2813 a, g dbSNP:78974571
2820 2820 a, c dbSNP:574499830
2870 2870 a, c dbSNP:138499283
2898 2898 a, g dbSNP:141979564
2969 2969 a, g dbSNP:576457297
3032 3032 -, ataa dbSNP:5821329
3047 3047 -, aata dbSNP:3033496
3054 3054 a, t dbSNP:545389486
3062 3062 c, t dbSNP:563392407
3079 3079 g, t dbSNP:562482480
3234 3234 -, at dbSNP:374788278
3235 3235 -, atat dbSNP:71355185
3235 3235 -, at dbSNP:147094207
3238 3238 -, atat, t dbSNP:71150616
3260 3260 -, ta dbSNP:547983914
3261 3261 -, tatatata dbSNP:56105625
3262 3262 a, c dbSNP:201283016
3274 3274 -, ct dbSNP:113244672
3275 3275 a, t dbSNP:111558928

Target ORF information:

RefSeq Version XM_005257837
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257837

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50822
Accession Version XM_011525492.1
Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011523794.1
CDS25..1665
Misc Feature(1)220..789(+)
Misc Feature(2)286..777(+)
Misc Feature(3)358..627(+)
Translation
Position Chain Variation Link
37 37 -, a dbSNP:769433167
40 40 a, g dbSNP:117410176
41 41 c, g dbSNP:3744448
42 42 c, t dbSNP:752448330
47 47 c, t dbSNP:776660359
48 48 c, t dbSNP:777880490
49 49 c, g dbSNP:748755835
58 58 a, g dbSNP:756802394
65 65 c, t dbSNP:778604942
66 66 c, t dbSNP:745523181
67 67 c, t dbSNP:771639735
87 87 c, g dbSNP:775860775
88 88 c, g dbSNP:747369610
89 89 c, g dbSNP:769157857
95 95 c, t dbSNP:573485618
118 118 c, t dbSNP:762348865
127 127 g, t dbSNP:765299000
128 128 c, t dbSNP:148424252
170 170 g, t dbSNP:558901097
174 174 c, t dbSNP:763175971
178 178 a, g dbSNP:766513791
180 180 a, c dbSNP:752496226
183 183 c, t dbSNP:755880238
185 185 c, t dbSNP:764025450
189 189 -, cgc dbSNP:772891363
196 196 a, g dbSNP:575407008
199 199 a, g dbSNP:80164240
208 208 c, t dbSNP:104894648
214 214 a, t dbSNP:554741896
219 219 a, g dbSNP:759838845
231 231 c, g dbSNP:763974215
232 232 a, g dbSNP:753701583
233 233 a, g dbSNP:761779230
250 250 c, g dbSNP:765300595
258 258 g, t dbSNP:182844772
273 273 a, g dbSNP:35070263
279 279 a, c dbSNP:779655731
282 282 a, g dbSNP:751168165
287 287 c, t dbSNP:754685175
300 300 g, t dbSNP:3744447
330 330 a, g dbSNP:370004814
337 337 a, g dbSNP:753009856
340 340 a, g dbSNP:200429513
347 347 a, g dbSNP:778236633
351 351 c, t dbSNP:749751811
359 359 a, g dbSNP:377571192
367 367 c, t dbSNP:778794211
373 373 a, g dbSNP:745977352
377 377 a, g dbSNP:772372461
383 383 c, t dbSNP:200977400
390 390 c, t dbSNP:199796272
391 391 a, g dbSNP:769475203
394 394 a, g dbSNP:773120525
400 400 c, t dbSNP:762743351
419 419 a, g dbSNP:375329209
423 423 a, t dbSNP:147644451
427 427 a, g dbSNP:774245437
430 430 c, g dbSNP:759509229
448 448 c, t dbSNP:767009862
456 456 g, t dbSNP:777426077
476 476 a, c dbSNP:775037825
479 479 c, t dbSNP:760185283
480 480 a, g, t dbSNP:375043323
486 486 c, t dbSNP:757620452
491 491 c, t dbSNP:765562149
511 511 a, c, t dbSNP:141536152
512 512 a, g dbSNP:780227761
519 519 c, g dbSNP:747092422
525 525 c, t dbSNP:755113753
531 531 c, g dbSNP:781496033
534 534 a, g dbSNP:748363362
556 556 c, g dbSNP:367714205
568 568 a, g dbSNP:778746605
570 570 c, t dbSNP:745791563
578 578 c, t dbSNP:771949985
584 584 a, c dbSNP:779946817
597 597 g, t dbSNP:199562610
606 606 a, g dbSNP:370177908
618 618 c, t dbSNP:147048921
619 619 a, g dbSNP:151058527
636 636 c, t dbSNP:140971014
645 645 c, t dbSNP:150224277
646 646 a, g dbSNP:78640841
655 655 a, g dbSNP:766896121
672 672 c, t dbSNP:752070991
676 676 a, c, g dbSNP:201328365
682 682 a, c dbSNP:752732593
686 686 a, c, g dbSNP:375091484
711 711 c, g dbSNP:754110365
723 723 c, t dbSNP:758160320
767 767 g, t dbSNP:28938474
768 768 a, g dbSNP:368135922
782 782 a, t dbSNP:754907416
783 783 a, c, t dbSNP:370445458
793 793 c, t dbSNP:755652052
796 796 c, t dbSNP:138858259
797 797 a, g dbSNP:748931901
804 804 c, t dbSNP:34495182
805 805 a, c dbSNP:774711057
806 806 a, g dbSNP:746247653
809 809 a, t dbSNP:772580057
811 811 a, c, t dbSNP:775830551
828 828 c, t dbSNP:145889644
829 829 a, g dbSNP:572207593
842 842 c, g dbSNP:764158066
843 843 c, t dbSNP:372765864
847 847 a, g, t dbSNP:541224543
852 852 a, g dbSNP:778553600
861 861 c, t dbSNP:750033041
864 864 c, t dbSNP:564100262
865 865 -, c dbSNP:766300906
868 868 c, t dbSNP:73334576
869 869 c, g dbSNP:747409546
870 870 a, c, g, t dbSNP:769106353
879 879 a, g dbSNP:550334195
880 880 a, g dbSNP:773396940
881 881 c, t dbSNP:201395852
886 886 c, g dbSNP:193204039
887 887 c, t dbSNP:759150888
891 891 c, t dbSNP:775385092
892 892 a, c, g, t dbSNP:760566882
899 899 c, t dbSNP:761870318
900 900 c, t dbSNP:142327430
906 906 c, t dbSNP:749954407
907 907 a, g dbSNP:143895322
920 920 a, c dbSNP:766046321
927 927 a, g dbSNP:751264961
939 939 c, t dbSNP:148616062
945 945 c, t dbSNP:141188668
946 946 a, g dbSNP:748587793
951 951 a, g dbSNP:113899943
956 956 c, t dbSNP:202061937
963 963 c, t dbSNP:777644345
964 964 a, g dbSNP:371242130
965 965 c, t dbSNP:3744438
966 966 a, g dbSNP:201565504
967 967 a, c, g dbSNP:746131244
970 970 c, g dbSNP:551973242
971 971 c, t dbSNP:200387033
972 972 a, g dbSNP:765251401
981 981 a, g dbSNP:772552261
987 987 a, c dbSNP:199780097
994 994 c, t dbSNP:765917350
997 997 c, t dbSNP:751211966
1002 1002 a, c dbSNP:370805904
1009 1009 a, g dbSNP:150233337
1011 1011 c, t dbSNP:767809364
1029 1029 c, t dbSNP:577197197
1030 1030 a, t dbSNP:756535438
1043 1043 a, g dbSNP:778365568
1051 1051 a, g dbSNP:767227688
1055 1055 a, c dbSNP:139626296
1056 1056 a, c, t dbSNP:761077197
1057 1057 c, t dbSNP:754364356
1058 1058 a, g dbSNP:200962877
1066 1066 g, t dbSNP:779069209
1069 1069 c, t dbSNP:750564852
1072 1072 c, g dbSNP:145267704
1082 1082 c, g, t dbSNP:61739275
1083 1083 a, t dbSNP:747237133
1084 1084 a, t dbSNP:769630174
1088 1088 a, g dbSNP:777491054
1096 1096 g, t dbSNP:749162745
1097 1097 c, t dbSNP:147641113
1098 1098 c, t dbSNP:115169711
1106 1106 c, t dbSNP:149749116
1107 1107 a, g dbSNP:200518950
1109 1109 c, g, t dbSNP:775175284
1110 1110 c, g dbSNP:61739274
1122 1122 c, g dbSNP:754259211
1129 1129 c, t dbSNP:372115732
1130 1130 a, g dbSNP:765868170
1131 1131 c, t dbSNP:751019794
1132 1132 -, c dbSNP:754897911
1137 1137 a, c, g, t dbSNP:140385970
1138 1138 a, c, g dbSNP:199727670
1139 1139 a, c, t dbSNP:143267075
1147 1147 a, g dbSNP:770803206
1171 1171 c, t dbSNP:778799625
1172 1172 a, c dbSNP:536406541
1174 1174 c, t dbSNP:577394793
1182 1182 a, g dbSNP:774920212
1188 1188 a, c dbSNP:760308988
1191 1191 c, g, t dbSNP:768224294
1224 1224 c, t dbSNP:375128049
1225 1225 a, g dbSNP:765739860
1233 1233 c, t dbSNP:773853893
1234 1234 a, g dbSNP:146829316
1236 1236 a, g dbSNP:766428417
1239 1239 a, g dbSNP:751591708
1241 1241 c, g dbSNP:140662248
1244 1244 g, t dbSNP:145825881
1251 1251 c, t dbSNP:7218485
1252 1252 a, g dbSNP:757013870
1255 1255 c, t dbSNP:778744732
1262 1262 c, g dbSNP:745791793
1264 1264 c, t dbSNP:758280314
1268 1268 c, t dbSNP:779577386
1269 1269 a, g dbSNP:143027472
1272 1272 g, t dbSNP:768262582
1278 1278 c, t dbSNP:150277085
1287 1287 a, g dbSNP:138356325
1299 1299 a, g dbSNP:370684273
1302 1302 a, g dbSNP:370669286
1306 1306 a, g dbSNP:773729072
1307 1307 c, g dbSNP:763447195
1314 1314 c, t dbSNP:766821708
1318 1318 a, g dbSNP:774437880
1323 1323 a, g dbSNP:759508725
1325 1325 c, t dbSNP:767623150
1326 1326 a, g dbSNP:752909576
1328 1328 c, t dbSNP:756385029
1330 1330 a, g dbSNP:764952897
1341 1341 a, g dbSNP:373379452
1344 1344 c, t dbSNP:758307212
1354 1354 c, t dbSNP:149248181
1356 1356 c, t dbSNP:529644070
1359 1359 a, g dbSNP:754444689
1367 1367 c, t dbSNP:780910584
1368 1368 c, t dbSNP:199998292
1369 1369 a, g dbSNP:769578381
1373 1373 c, t dbSNP:773667933
1376 1376 a, c, t dbSNP:376613781
1386 1386 a, g dbSNP:566653409
1387 1387 c, t dbSNP:370445781
1388 1388 a, g dbSNP:373815870
1395 1395 c, g dbSNP:772123763
1404 1404 c, t dbSNP:200860672
1405 1405 a, g dbSNP:760819355
1411 1411 a, c dbSNP:764323057
1418 1418 a, g dbSNP:754139697
1420 1420 c, t dbSNP:532216691
1425 1425 a, g dbSNP:766266920
1433 1433 c, g dbSNP:751441900
1436 1436 a, g dbSNP:75619997
1441 1441 a, g dbSNP:754983531
1443 1443 g, t dbSNP:780786210
1446 1446 c, g dbSNP:752282577
1460 1460 c, t dbSNP:755776465
1473 1473 c, t dbSNP:2270150
1474 1474 c, t dbSNP:749007884
1475 1475 a, g dbSNP:139609877
1477 1477 a, g dbSNP:779390654
1480 1480 c, t dbSNP:746302650
1481 1481 a, g dbSNP:772572337
1487 1487 c, t dbSNP:776086286
1488 1488 c, t dbSNP:760722978
1491 1491 a, c, t dbSNP:143608018
1492 1492 a, g dbSNP:762155896
1498 1498 c, t dbSNP:766033021
1500 1500 c, t dbSNP:147183215
1501 1501 c, t dbSNP:751377938
1510 1510 c, t dbSNP:759509799
1512 1512 c, t dbSNP:767580732
1513 1513 a, g dbSNP:140527546
1521 1521 c, g, t dbSNP:755651888
1523 1523 a, g dbSNP:753417448
1528 1528 c, t dbSNP:756946831
1532 1532 a, g dbSNP:537917982
1539 1539 a, g dbSNP:117324438
1545 1545 c, g dbSNP:772474213
1547 1547 c, t dbSNP:777040245
1548 1548 a, g dbSNP:188952741
1552 1552 c, t dbSNP:181321459
1568 1568 a, g dbSNP:768608302
1578 1578 c, t dbSNP:776788981
1582 1582 a, t dbSNP:748255000
1590 1590 -, ctt dbSNP:781174408
1593 1593 -, ctc dbSNP:747908174
1603 1603 c, t dbSNP:200436565
1604 1604 a, g dbSNP:773964621
1608 1608 a, c dbSNP:151223901
1613 1613 c, t dbSNP:767458043
1619 1619 a, g dbSNP:28936696
1634 1634 a, t dbSNP:372012864
1637 1637 a, g dbSNP:760750802
1638 1638 a, g dbSNP:763453760
1641 1641 c, t dbSNP:753391567
1642 1642 g, t dbSNP:756819237
1647 1647 a, g dbSNP:762062413
1656 1656 c, t dbSNP:750116867
1659 1659 c, t dbSNP:545088929
1660 1660 a, g dbSNP:758758874
1669 1669 c, t dbSNP:780456576
1672 1672 c, g, t dbSNP:149961771
1673 1673 a, g dbSNP:781095471
1676 1676 c, t dbSNP:748286433
1682 1682 a, g dbSNP:769809385
1685 1685 a, g dbSNP:773533114
1686 1686 c, t dbSNP:368731674
1689 1689 c, t dbSNP:144878479
1690 1690 a, g dbSNP:185650741
1695 1695 c, t dbSNP:760621927
1696 1696 a, g dbSNP:544357700
1711 1711 c, t dbSNP:775955071
1720 1720 a, t dbSNP:560799982
1744 1744 a, g dbSNP:529742456
1753 1753 a, g dbSNP:539840563
1762 1762 -, gt, gtgtgtgt dbSNP:149977669
1764 1764 a, t dbSNP:79894176
1792 1792 -, tg dbSNP:71885970
1802 1802 -, gt, gtgtgtgt, ta dbSNP:60535903
1803 1803 a, g dbSNP:77924694
1808 1808 c, t dbSNP:149034024
1813 1813 a, g dbSNP:111840354
1877 1877 -, cac dbSNP:756840229
1935 1935 c, t dbSNP:138718626
1951 1951 c, t dbSNP:531385585
1952 1952 g, t dbSNP:548690089
1964 1964 a, g dbSNP:568457409
1978 1978 a, c dbSNP:141279587
1992 1992 a, g dbSNP:58804550
1994 1994 g, t dbSNP:571164199
2071 2071 c, t dbSNP:77786466
2073 2073 -, gaa dbSNP:771729163
2081 2081 c, t dbSNP:556881465
2083 2083 c, t dbSNP:576739539
2107 2107 g, t dbSNP:180857223
2111 2111 c, t dbSNP:753474884
2122 2122 a, t dbSNP:559990367
2166 2166 a, t dbSNP:554691569
2175 2175 a, g dbSNP:561282671
2177 2177 c, t dbSNP:528911904
2218 2218 a, g dbSNP:574411058
2227 2227 c, t dbSNP:540228848
2246 2246 a, t dbSNP:559868966
2295 2295 c, t dbSNP:542201906
2321 2321 c, t dbSNP:187653934
2366 2366 -, t dbSNP:372489230
2370 2370 -, g dbSNP:370185892
2371 2371 -, tt dbSNP:745609877
2372 2372 -, g dbSNP:111608426
2372 2372 g, t dbSNP:3744437
2373 2373 -, t dbSNP:3833137
2387 2387 a, c dbSNP:377432317
2424 2424 a, g dbSNP:59569849
2431 2431 a, c dbSNP:191996071
2437 2437 c, t dbSNP:183868550
2474 2474 a, g dbSNP:187454602
2475 2475 c, t dbSNP:541669280
2506 2506 a, t dbSNP:547791456
2508 2508 a, g dbSNP:3744436
2528 2528 a, g dbSNP:3744435
2649 2649 a, g dbSNP:550543497
2666 2666 a, g dbSNP:190368544
2669 2669 c, t dbSNP:370432592
2673 2673 a, g dbSNP:78974571
2680 2680 a, c dbSNP:574499830
2730 2730 a, c dbSNP:138499283
2758 2758 a, g dbSNP:141979564
2829 2829 a, g dbSNP:576457297
2892 2892 -, ataa dbSNP:5821329
2907 2907 -, aata dbSNP:3033496
2914 2914 a, t dbSNP:545389486
2922 2922 c, t dbSNP:563392407
2939 2939 g, t dbSNP:562482480
3094 3094 -, at dbSNP:374788278
3095 3095 -, atat dbSNP:71355185
3095 3095 -, at dbSNP:147094207
3098 3098 -, atat, t dbSNP:71150616
3120 3120 -, ta dbSNP:547983914
3121 3121 -, tatatata dbSNP:56105625
3122 3122 a, c dbSNP:201283016
3134 3134 -, ct dbSNP:113244672
3135 3135 a, t dbSNP:111558928

Target ORF information:

RefSeq Version XM_011525492
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens T-box 4 (TBX4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011525492

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50822
Accession Version XM_011525493.1
Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product T-box transcription factor TBX4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010783.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCTGCAGG ATAAGGGCCT GTCCGAGAGC GAGGAGGCCT TCCGGGCCCC GGGCCCAGCG 
CTCGGAGAGG CCAGCGCAGC CAACGCCCCC GAGCCCGCGC TGGCAGCGCC GGGCCTCAGC
GGAGCCGCGC TAGGCAGCCC CCCGGGACCC GGGGCCGACG TCGTCGCCGC CGCCGCCGCG
GAGCAGACCA TCGAGAACAT CAAGGTGGGG CTGCATGAGA AGGAGCTCTG GAAGAAGTTC
CACGAGGCGG GCACCGAGAT GATCATCACT AAGGCTGGCA GGAGGATGTT CCCCAGCTAC
AAGGTAAAAG TCACAGGCAT GAACCCCAAG ACCAAGTATA TCCTGCTGAT TGACATTGTC
CCTGCCGATG ACCATCGCTA CAAGTTCTGT GACAACAAAT GGATGGTGGC AGGGAAGGCT
GAGCCAGCCA TGCCAGGAAG GCTGTATGTC CACCCGGATT CTCCTGCCAC AGGAGCCCAC
TGGATGCGGC AGCTGGTCTC CTTCCAGAAG CTGAAGCTGA CAAACAACCA CCTGGACCCC
TTTGGCCATA TCATCCTCAA CTCTATGCAC AAGTACCAGC CGCGGCTCCA CATCGTTAAG
GCTGATGAGA ACAATGCTTT CGGCTCCAAA AACACTGCTT TCTGCACCCA CGTGTTCCCA
GAGACCTCCT TCATCTCTGT GACCTCCTAC CAGAATCACA AGATCACCCA GCTGAAAATT
GAGAACAACC CTTTTGCCAA GGGATTCCGG GGCAGTGATG ACAGTGACCT GCGTGTGGCC
CGACTGCAGA GCAAAGAATA CCCCGTGATT TCCAAAAGCA TCATGAGGCA GAGGCTCATC
TCCCCCCAGC TCTCAGCCAC ACCGGACGTG GGCCCCCTGC TCGGCACCCA CCAGGCACTC
CAGCACTACC AGCACGAGAA CGGGGCACAC TCACAGCTCG CGGAGCCGCA GGACCTGCCC
CTCAGCACCT TTCCCACCCA GAGGGACTCA AGCCTCTTCT ATCACTGCCT GAAAAGACGA
GCAGACGGTA CCCGCCACCT GGACTTACCT TGCAAGCGAT CCTATCTGGA AGCCCCCTCT
TCGGTGGGGG AGGATCACTA TTTCCGTTCC CCCCCTCCCT ACGACCAGCA AATGCTGAGC
CCCTCCTACT GCAGTGAGGT GACCCCCAGA GAAGCATGTA TGTACTCAGG TTCAGGGCCC
GAGATTGCCG GGGTGTCTGG GGTGGACGAC CTGCCCCCAC CTCCGCTGAG CTGTAACATG
TGGACTTCAG TGTCGCCGTA CACCAGCTAT AGCGTGCAGA CGATGGAGAC TGTGCCGTAC
CAGCCCTTCC CCACGCACTT CACCGCCACC ACCATGATGC CGCGGCTGCC CACCCTCTCC
GCTCAGAGCT CCCAGCCACC AGGAAATGCC CACTTTAGTG TCTACAATCA GCTCTCCCAG
TCTCAGGTCC GAGAGCGGGG GCCCAGCGCC TCATTCCCAA GAGAGCGCGG CCTCCCCCAA
GGGTGTGAGA GGAAGCCACC CTCGCCACAT CTAAATGCTG CCAATGAGTT TCTCTACTCT
CAAACCTTCT CCTTGTCCCG AGAATCTTCC TTACAGTACC ATTCAGGAAT GGGGACTGTG
GAGAACTGGA CTGACGGATG A
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011523795.1
CDS25..1665
Misc Feature(1)220..789(+)
Misc Feature(2)286..777(+)
Misc Feature(3)358..627(+)
Translation
Position Chain Variation Link
37 37 -, a dbSNP:769433167
40 40 a, g dbSNP:117410176
41 41 c, g dbSNP:3744448
42 42 c, t dbSNP:752448330
47 47 c, t dbSNP:776660359
48 48 c, t dbSNP:777880490
49 49 c, g dbSNP:748755835
58 58 a, g dbSNP:756802394
65 65 c, t dbSNP:778604942
66 66 c, t dbSNP:745523181
67 67 c, t dbSNP:771639735
87 87 c, g dbSNP:775860775
88 88 c, g dbSNP:747369610
89 89 c, g dbSNP:769157857
95 95 c, t dbSNP:573485618
118 118 c, t dbSNP:762348865
127 127 g, t dbSNP:765299000
128 128 c, t dbSNP:148424252
170 170 g, t dbSNP:558901097
174 174 c, t dbSNP:763175971
178 178 a, g dbSNP:766513791
180 180 a, c dbSNP:752496226
183 183 c, t dbSNP:755880238
185 185 c, t dbSNP:764025450
189 189 -, cgc dbSNP:772891363
196 196 a, g dbSNP:575407008
199 199 a, g dbSNP:80164240
208 208 c, t dbSNP:104894648
214 214 a, t dbSNP:554741896
219 219 a, g dbSNP:759838845
231 231 c, g dbSNP:763974215
232 232 a, g dbSNP:753701583
233 233 a, g dbSNP:761779230
250 250 c, g dbSNP:765300595
258 258 g, t dbSNP:182844772
273 273 a, g dbSNP:35070263
279 279 a, c dbSNP:779655731
282 282 a, g dbSNP:751168165
287 287 c, t dbSNP:754685175
300 300 g, t dbSNP:3744447
330 330 a, g dbSNP:370004814
337 337 a, g dbSNP:753009856
340 340 a, g dbSNP:200429513
347 347 a, g dbSNP:778236633
351 351 c, t dbSNP:749751811
359 359 a, g dbSNP:377571192
367 367 c, t dbSNP:778794211
373 373 a, g dbSNP:745977352
377 377 a, g dbSNP:772372461
383 383 c, t dbSNP:200977400
390 390 c, t dbSNP:199796272
391 391 a, g dbSNP:769475203
394 394 a, g dbSNP:773120525
400 400 c, t dbSNP:762743351
419 419 a, g dbSNP:375329209
423 423 a, t dbSNP:147644451
427 427 a, g dbSNP:774245437
430 430 c, g dbSNP:759509229
448 448 c, t dbSNP:767009862
456 456 g, t dbSNP:777426077
476 476 a, c dbSNP:775037825
479 479 c, t dbSNP:760185283
480 480 a, g, t dbSNP:375043323
486