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MAGED1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAGED1
Entrez Gene ID 9500
Full Name melanoma antigen family D1
Synonyms DLXIN-1, NRAGE
General protein information
Preferred Names
melanoma-associated antigen D1
Names
melanoma-associated antigen D1
MAGE-D1 antigen
MAGE tumor antigen CCF
neurotrophin receptor-interacting MAGE homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.23

Summary This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300224

Disorder Html:





The following MAGED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18181 XM_011530835 PREDICTED: Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$391.30
$559.00
OHu18181 NM_006986 Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$391.30
$559.00
OHu18181 NM_001005332 Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$391.30
$559.00
OHu03665 NM_001005333 Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$475.30
$679.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu18181
Accession Version XM_011530835.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011630.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_011529137.1
CDS128..2464
Misc Feature(1)1559..2068(+)
Translation
Position Chain Variation Link
471 471 a, g, t dbSNP:113823505
472 472 a, g dbSNP:200269066
571 571 c, t dbSNP:144264968
839 839 a, c dbSNP:12689461
941 941 a, g dbSNP:41307638
2119 2119 a, t dbSNP:201229700
2186 2186 a, c dbSNP:3199687
2487 2487 c, t dbSNP:58080956

Target ORF information:

RefSeq Version XM_011530835
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530835

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Accession Version NM_006986.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D1 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2, CD387872.1, BF529661.1, BC014070.2 and AF132205.1. On Sep 24, 2004 this sequence version replaced gi:14195633. Summary: This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF258554.1, AF217963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_008917.3
CDS191..2527
Misc Feature(1)146..148(+)
Misc Feature(2)464..466(+)
Misc Feature(3)464..466(+)
Misc Feature(4)464..466(+)
Misc Feature(5)1076..1522(+)
Misc Feature(6)1622..2131(+)
Exon (1)1..154
Gene:MAGED1
Gene Synonym:
Exon (2)155..235
Gene:MAGED1
Gene Synonym:
Exon (3)236..943
Gene:MAGED1
Gene Synonym:
Exon (4)944..1612
Gene:MAGED1
Gene Synonym:
Exon (5)1613..1676
Gene:MAGED1
Gene Synonym:
Exon (6)1677..1756
Gene:MAGED1
Gene Synonym:
Exon (7)1757..1848
Gene:MAGED1
Gene Synonym:
Exon (8)1849..1928
Gene:MAGED1
Gene Synonym:
Exon (9)1929..1971
Gene:MAGED1
Gene Synonym:
Exon (10)1972..2034
Gene:MAGED1
Gene Synonym:
Exon (11)2035..2149
Gene:MAGED1
Gene Synonym:
Exon (12)2150..2535
Gene:MAGED1
Gene Synonym:
Exon (13)2536..2744
Gene:MAGED1
Gene Synonym:
Translation
Position Chain Variation Link
534 534 a, g, t dbSNP:113823505
535 535 a, g dbSNP:200269066
634 634 c, t dbSNP:144264968
902 902 a, c dbSNP:12689461
1004 1004 a, g dbSNP:41307638
2182 2182 a, t dbSNP:201229700
2249 2249 a, c dbSNP:3199687
2550 2550 c, t dbSNP:58080956

Target ORF information:

RefSeq Version NM_006986
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006986

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Accession Version NM_001005332.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D1 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG819042.1, BC014070.2 and AF132205.1. This sequence is a reference standard in the RefSeqGene project. Summary: This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF258554.1, AF217963.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001005332.1
CDS96..2432
Misc Feature(1)369..371(+)
Misc Feature(2)369..371(+)
Misc Feature(3)369..371(+)
Misc Feature(4)981..1427(+)
Misc Feature(5)1527..2036(+)
Exon (1)1..59
Gene:MAGED1
Gene Synonym:
Exon (2)60..140
Gene:MAGED1
Gene Synonym:
Exon (3)141..848
Gene:MAGED1
Gene Synonym:
Exon (4)849..1517
Gene:MAGED1
Gene Synonym:
Exon (5)1518..1581
Gene:MAGED1
Gene Synonym:
Exon (6)1582..1661
Gene:MAGED1
Gene Synonym:
Exon (7)1662..1753
Gene:MAGED1
Gene Synonym:
Exon (8)1754..1833
Gene:MAGED1
Gene Synonym:
Exon (9)1834..1876
Gene:MAGED1
Gene Synonym:
Exon (10)1877..1939
Gene:MAGED1
Gene Synonym:
Exon (11)1940..2054
Gene:MAGED1
Gene Synonym:
Exon (12)2055..2440
Gene:MAGED1
Gene Synonym:
Exon (13)2441..2649
Gene:MAGED1
Gene Synonym:
Translation
Position Chain Variation Link
439 439 a, g, t dbSNP:113823505
440 440 a, g dbSNP:200269066
539 539 c, t dbSNP:144264968
807 807 a, c dbSNP:12689461
909 909 a, g dbSNP:41307638
2087 2087 a, t dbSNP:201229700
2154 2154 a, c dbSNP:3199687
2455 2455 c, t dbSNP:58080956

Target ORF information:

RefSeq Version NM_001005332
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001005332

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03665
Accession Version NM_001005333.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product melanoma-associated antigen D1 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2, BC014070.2 and AF132205.1. Summary: This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014070.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001005333.1
CDS191..2695
Misc Feature(1)146..148(+)
Misc Feature(2)632..634(+)
Misc Feature(3)632..634(+)
Misc Feature(4)1790..2299(+)
Exon (1)1..154
Gene:MAGED1
Gene Synonym:
Exon (2)155..235
Gene:MAGED1
Gene Synonym:
Exon (3)236..403
Gene:MAGED1
Gene Synonym:
Exon (4)404..1111
Gene:MAGED1
Gene Synonym:
Exon (5)1112..1780
Gene:MAGED1
Gene Synonym:
Exon (6)1781..1844
Gene:MAGED1
Gene Synonym:
Exon (7)1845..1924
Gene:MAGED1
Gene Synonym:
Exon (8)1925..2016
Gene:MAGED1
Gene Synonym:
Exon (9)2017..2096
Gene:MAGED1
Gene Synonym:
Exon (10)2097..2139
Gene:MAGED1
Gene Synonym:
Exon (11)2140..2202
Gene:MAGED1
Gene Synonym:
Exon (12)2203..2317
Gene:MAGED1
Gene Synonym:
Exon (13)2318..2703
Gene:MAGED1
Gene Synonym:
Exon (14)2704..2912
Gene:MAGED1
Gene Synonym:
Translation
Position Chain Variation Link
702 702 a, g, t dbSNP:113823505
703 703 a, g dbSNP:200269066
802 802 c, t dbSNP:144264968
1070 1070 a, c dbSNP:12689461
1172 1172 a, g dbSNP:41307638
2350 2350 a, t dbSNP:201229700
2417 2417 a, c dbSNP:3199687
2718 2718 c, t dbSNP:58080956

Target ORF information:

RefSeq Version NM_001005333
Organism Homo sapiens (human)
Definition Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001005333

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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