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CD40 CD40 molecule, TNF receptor superfamily member 5 [Homo sapiens (human)]

Gene Symbol CD40
Entrez Gene ID 958
Full Name CD40 molecule, TNF receptor superfamily member 5
Synonyms Bp50, CDW40, TNFRSF5, p50
General protein information
Preferred Names
tumor necrosis factor receptor superfamily member 5
Names
tumor necrosis factor receptor superfamily member 5
CD40L receptor
CD40 type II isoform
B cell-associated molecule
B cell surface antigen CD40
B-cell surface antigen CD40
CD40 antigen (TNF receptor superfamily member 5)
tumor necrosis factor receptor superfamily, member 5
nerve growth factor receptor-related B-lymphocyte activation molecule
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q12-q13.2

Summary This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

109535

Disorder Html: Immunodeficiency with hyper-IgM, type 3, 606843 (3)





The following CD40 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD40 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu53155 XM_005260617 PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu53158 XM_005260619 PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu56743 XM_011529109 PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $99
OHu20663 NM_001250 Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$199
OHu53156 NM_001302753 Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OHu12389 NM_152854 Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OHu53155
Accession Version XM_005260617.2
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530418532. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAGTGA GTCCTCAGAA
AAGGTGGCCA AGAAGCCAAC CAATAAGGCC CCCCACCCCA AGCAGGAACC CCAGGAGATC
AATTTTCCCG ACGATCTTCC TGGCTCCAAC ACTGCTGCTC CAGTGCAGGA GACTTTACAT
GGATGCCAAC CGGTCACCCA GGAGGATGGC AAAGAGAGTC GCATCTCAGT GCAGGAGAGA
CAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005260674.1
CDS94..939
Misc Feature(1)169..651(+)
Misc Feature(2)169..270(+)
Misc Feature(3)277..402(+)
Misc Feature(4)286..450(+)
Misc Feature(5)406..522(+)
Misc Feature(6)529..651(+)
Translation
Position Chain Variation Link
1 1 c, t dbSNP:538304811
23 23 c, g dbSNP:556909578
25 25 -, t dbSNP:11569300
27 27 g, t dbSNP:575088069
33 33 g, t dbSNP:542745249
35 35 g, t dbSNP:554674186
39 39 c, g dbSNP:573086622
46 46 a, g dbSNP:746607340
47 47 c, g dbSNP:372855582
52 52 c, g dbSNP:768399532
53 53 c, t dbSNP:11569301
56 56 c, g dbSNP:762302983
57 57 c, g dbSNP:373167365
60 60 c, g dbSNP:773732954
61 61 c, t dbSNP:377285521
63 63 c, g dbSNP:766418920
64 64 a, t dbSNP:751711543
71 71 c, t dbSNP:755142543
74 74 c, g dbSNP:371166508
78 78 c, t dbSNP:753511518
81 81 g, t dbSNP:200981957
83 83 c, g dbSNP:778942629
91 91 a, g dbSNP:140399416
92 92 a, c dbSNP:758449676
93 93 c, t dbSNP:1883832
104 104 g, t dbSNP:746583774
113 113 a, c dbSNP:768187312
115 115 g, t dbSNP:113207193
121 121 a, c dbSNP:748416131
142 142 g, t dbSNP:770177808
147 147 c, g dbSNP:771363187
149 149 a, g dbSNP:774936391
158 158 c, t dbSNP:759483970
166 166 g, t dbSNP:147677886
168 168 a, g dbSNP:775472655
177 177 a, g dbSNP:760957101
188 188 c, t dbSNP:764464873
196 196 a, g dbSNP:750234130
197 197 a, g dbSNP:762843067
208 208 c, t dbSNP:766167612
216 216 c, t dbSNP:751549823
240 240 c, t dbSNP:541686651
249 249 a, g dbSNP:767422189
258 258 c, g, t dbSNP:752136889
264 264 a, g dbSNP:115724543
279 279 c, t dbSNP:753518969
280 280 a, g dbSNP:756898850
288 288 a, c dbSNP:202208745
294 294 c, t dbSNP:746276099
297 297 a, g dbSNP:142258778
300 300 a, c dbSNP:371950759
302 302 a, c dbSNP:747024806
323 323 g, t dbSNP:17855908
324 324 c, t dbSNP:768876770
327 327 a, c dbSNP:17177493
332 332 a, g dbSNP:376829285
340 340 c, t dbSNP:28931586
342 342 c, t dbSNP:776893342
345 345 c, t dbSNP:748382649
346 346 a, c dbSNP:769971386
352 352 c, g dbSNP:768637864
361 361 c, t dbSNP:144542285
362 362 a, g dbSNP:761229326
364 364 a, g dbSNP:764882779
365 365 c, t dbSNP:750063104
381 381 c, g, t dbSNP:763334863
384 384 a, g dbSNP:752031207
386 386 a, t dbSNP:755540822
405 405 c, t dbSNP:781766854
415 415 a, g dbSNP:752764084
418 418 c, t dbSNP:756171667
420 420 a, g dbSNP:778017892
422 422 a, g dbSNP:564200263
423 423 c, t dbSNP:771189629
428 428 a, c, t dbSNP:779810076
429 429 a, c, g dbSNP:267605960
437 437 a, c, t dbSNP:537489349
438 438 c, t dbSNP:147816161
439 439 c, t dbSNP:772829518
447 447 c, g dbSNP:555476733
451 451 g, t dbSNP:762684377
458 458 a, g dbSNP:766205073
461 461 a, g dbSNP:751910371
464 464 c, t dbSNP:11569321
468 468 c, t dbSNP:768090085
470 470 c, t dbSNP:753176464
471 471 a, g, t dbSNP:150312341
474 474 c, t dbSNP:763263083
475 475 a, g dbSNP:754006524
477 477 a, c dbSNP:757465117
483 483 g, t dbSNP:1801293
493 493 a, c, g dbSNP:61760052
494 494 c, t dbSNP:371997367
505 505 g, t dbSNP:368921167
506 506 c, t dbSNP:755938402
516 516 c, t dbSNP:777779940
522 522 c, t dbSNP:373653555
523 523 a, g dbSNP:770600305
528 528 c, t dbSNP:376668410
537 537 c, t dbSNP:745496687
540 540 c, t dbSNP:771812128
551 551 a, g, t dbSNP:745551504
558 558 a, g dbSNP:573253733
561 561 c, t dbSNP:764447217
562 562 a, g dbSNP:777145547
567 567 a, c, t dbSNP:79661585
591 591 c, g dbSNP:144600981
593 593 a, g dbSNP:779766201
601 601 a, g dbSNP:747283803
609 609 c, g dbSNP:769096290
610 610 g, t dbSNP:777019036
623 623 a, c dbSNP:762283441
624 624 a, g dbSNP:374572404
646 646 a, g dbSNP:773211027
655 655 c, t dbSNP:757943526
656 656 a, c, t dbSNP:779532941
660 660 a, g dbSNP:768240688
665 665 a, g dbSNP:781521147
678 678 c, g dbSNP:748519791
693 693 c, g dbSNP:537635771
699 699 c, t dbSNP:7273698
703 703 a, g dbSNP:143037975
712 712 a, g dbSNP:762902889
714 714 c, t dbSNP:535886332
715 715 a, g dbSNP:199581355
716 716 c, t dbSNP:759793763
718 718 c, t dbSNP:767920606
726 726 a, g dbSNP:776500140
747 747 c, t dbSNP:761711766
758 758 g, t dbSNP:766292495
760 760 a, g dbSNP:368619894
764 764 a, g dbSNP:371799172
765 765 a, g dbSNP:754507112
768 768 a, g dbSNP:371353981
777 777 g, t dbSNP:767118191
782 782 c, t dbSNP:773019543
784 784 c, g, t dbSNP:11086998
786 786 c, t dbSNP:148342289
800 800 a, c dbSNP:774837924
807 807 a, g dbSNP:561338230
815 815 a, g dbSNP:768078284
822 822 c, t dbSNP:144466131
825 825 c, t dbSNP:147672904
826 826 g, t dbSNP:764257725
828 828 g, t dbSNP:754026379
833 833 c, t dbSNP:757516011
835 835 a, g dbSNP:779807906
848 848 c, t dbSNP:751437274
855 855 a, g dbSNP:376780996
856 856 a, g dbSNP:781200622
859 859 c, t dbSNP:199980487
860 860 a, g dbSNP:747561680
867 867 -, tt dbSNP:767094199
872 872 a, g dbSNP:769399729
876 876 a, c dbSNP:777475990
879 879 c, t dbSNP:748935911
882 882 a, g dbSNP:369693842
883 883 c, t dbSNP:774515822
884 884 c, t dbSNP:759961210
885 885 a, g dbSNP:150890139
888 888 c, t dbSNP:776099682
906 906 -, ag dbSNP:750063120
909 909 c, g dbSNP:761207472
914 914 a, g dbSNP:139300926
924 924 g, t dbSNP:753974727
955 955 -, g dbSNP:751626954
959 959 c, t dbSNP:762015042
967 967 c, t dbSNP:765500316
968 968 a, g dbSNP:529049263
989 989 c, t dbSNP:754823732
1001 1001 -, tgc dbSNP:755836869
1004 1004 c, t dbSNP:780950226
1011 1011 a, g dbSNP:752657781
1018 1018 c, t dbSNP:756178814
1019 1019 a, g dbSNP:777419143
1036 1036 c, g dbSNP:748815275
1037 1037 a, c dbSNP:770653224
1039 1039 c, t dbSNP:778653844
1058 1058 c, t dbSNP:746182207
1059 1059 a, g dbSNP:772447675
1066 1066 c, g dbSNP:775762947
1067 1067 c, t dbSNP:761154625
1068 1068 c, t dbSNP:769214684
1070 1070 a, c dbSNP:776624029
1072 1072 a, c, t dbSNP:761892578
1074 1074 c, g dbSNP:11569343
1117 1117 a, g dbSNP:565941222
1126 1126 g, t dbSNP:539494893
1136 1136 c, t dbSNP:369546762
1168 1168 a, g dbSNP:551789939
1203 1203 g, t dbSNP:570025940
1205 1205 g, t dbSNP:371282411
1217 1217 a, g dbSNP:537564344
1241 1241 c, g dbSNP:555863322
1245 1245 c, t dbSNP:772009573
1250 1250 c, t dbSNP:41282790
1255 1255 c, g dbSNP:11569344
1293 1293 a, g dbSNP:773341885
1311 1311 a, c dbSNP:117490488
1338 1338 a, g dbSNP:550037217
1397 1397 c, t dbSNP:747208252
1458 1458 c, t dbSNP:578190784
1486 1486 a, t dbSNP:184942163
1506 1506 g, t dbSNP:542031139

Target ORF information:

RefSeq Version XM_005260617
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260617

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAGTGA GTCCTCAGAA
AAGGTGGCCA AGAAGCCAAC CAATAAGGCC CCCCACCCCA AGCAGGAACC CCAGGAGATC
AATTTTCCCG ACGATCTTCC TGGCTCCAAC ACTGCTGCTC CAGTGCAGGA GACTTTACAT
GGATGCCAAC CGGTCACCCA GGAGGATGGC AAAGAGAGTC GCATCTCAGT GCAGGAGAGA
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53158
Accession Version XM_005260619.2
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530418536. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGGTCCCCA GGATCGGCTG
AGAGCCCTGG TGGTGATCCC CATCATCTTC GGGATCCTGT TTGCCATCCT CTTGGTGCTG
GTCTTTATCA GTGAGTCCTC AGAAAAGGTG GCCAAGAAGC CAACCAATAA GGCCCCCCAC
CCCAAGCAGG AACCCCAGGA GATCAATTTT CCCGACGATC TTCCTGGCTC CAACACTGCT
GCTCCAGTGC AGGAGACTTT ACATGGATGC CAACCGGTCA CCCAGGAGGA TGGCAAAGAG
AGTCGCATCT CAGTGCAGGA GAGACAGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_005260676.1
CDS95..784
Misc Feature(1)170..>499(+)
Misc Feature(2)278..403(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:538304811
24 24 c, g dbSNP:556909578
26 26 -, t dbSNP:11569300
28 28 g, t dbSNP:575088069
34 34 g, t dbSNP:542745249
36 36 g, t dbSNP:554674186
40 40 c, g dbSNP:573086622
47 47 a, g dbSNP:746607340
48 48 c, g dbSNP:372855582
53 53 c, g dbSNP:768399532
54 54 c, t dbSNP:11569301
57 57 c, g dbSNP:762302983
58 58 c, g dbSNP:373167365
61 61 c, g dbSNP:773732954
62 62 c, t dbSNP:377285521
64 64 c, g dbSNP:766418920
65 65 a, t dbSNP:751711543
72 72 c, t dbSNP:755142543
75 75 c, g dbSNP:371166508
79 79 c, t dbSNP:753511518
82 82 g, t dbSNP:200981957
84 84 c, g dbSNP:778942629
92 92 a, g dbSNP:140399416
93 93 a, c dbSNP:758449676
94 94 c, t dbSNP:1883832
105 105 g, t dbSNP:746583774
114 114 a, c dbSNP:768187312
116 116 g, t dbSNP:113207193
122 122 a, c dbSNP:748416131
143 143 g, t dbSNP:770177808
148 148 c, g dbSNP:771363187
150 150 a, g dbSNP:774936391
159 159 c, t dbSNP:759483970
167 167 g, t dbSNP:147677886
169 169 a, g dbSNP:775472655
178 178 a, g dbSNP:760957101
189 189 c, t dbSNP:764464873
197 197 a, g dbSNP:750234130
198 198 a, g dbSNP:762843067
209 209 c, t dbSNP:766167612
217 217 c, t dbSNP:751549823
241 241 c, t dbSNP:541686651
250 250 a, g dbSNP:767422189
259 259 c, g, t dbSNP:752136889
265 265 a, g dbSNP:115724543
280 280 c, t dbSNP:753518969
281 281 a, g dbSNP:756898850
289 289 a, c dbSNP:202208745
295 295 c, t dbSNP:746276099
298 298 a, g dbSNP:142258778
301 301 a, c dbSNP:371950759
303 303 a, c dbSNP:747024806
324 324 g, t dbSNP:17855908
325 325 c, t dbSNP:768876770
328 328 a, c dbSNP:17177493
333 333 a, g dbSNP:376829285
341 341 c, t dbSNP:28931586
343 343 c, t dbSNP:776893342
346 346 c, t dbSNP:748382649
347 347 a, c dbSNP:769971386
353 353 c, g dbSNP:768637864
362 362 c, t dbSNP:144542285
363 363 a, g dbSNP:761229326
365 365 a, g dbSNP:764882779
366 366 c, t dbSNP:750063104
382 382 c, g, t dbSNP:763334863
385 385 a, g dbSNP:752031207
387 387 a, t dbSNP:755540822
406 406 c, t dbSNP:781766854
416 416 a, g dbSNP:752764084
419 419 c, t dbSNP:756171667
421 421 a, g dbSNP:778017892
423 423 a, g dbSNP:564200263
424 424 c, t dbSNP:771189629
429 429 a, c, t dbSNP:779810076
430 430 a, c, g dbSNP:267605960
438 438 a, c, t dbSNP:537489349
439 439 c, t dbSNP:147816161
440 440 c, t dbSNP:772829518
448 448 c, g dbSNP:555476733
452 452 g, t dbSNP:762684377
459 459 a, g dbSNP:766205073
462 462 a, g dbSNP:751910371
465 465 c, t dbSNP:11569321
469 469 c, t dbSNP:768090085
471 471 c, t dbSNP:753176464
472 472 a, g, t dbSNP:150312341
475 475 c, t dbSNP:763263083
476 476 a, g dbSNP:754006524
478 478 a, c dbSNP:757465117
484 484 g, t dbSNP:1801293
494 494 a, c, g dbSNP:61760052
495 495 c, t dbSNP:371997367
500 500 c, t dbSNP:757943526
501 501 a, c, t dbSNP:779532941
505 505 a, g dbSNP:768240688
510 510 a, g dbSNP:781521147
523 523 c, g dbSNP:748519791
538 538 c, g dbSNP:537635771
544 544 c, t dbSNP:7273698
548 548 a, g dbSNP:143037975
557 557 a, g dbSNP:762902889
559 559 c, t dbSNP:535886332
560 560 a, g dbSNP:199581355
561 561 c, t dbSNP:759793763
563 563 c, t dbSNP:767920606
571 571 a, g dbSNP:776500140
592 592 c, t dbSNP:761711766
603 603 g, t dbSNP:766292495
605 605 a, g dbSNP:368619894
609 609 a, g dbSNP:371799172
610 610 a, g dbSNP:754507112
613 613 a, g dbSNP:371353981
622 622 g, t dbSNP:767118191
627 627 c, t dbSNP:773019543
629 629 c, g, t dbSNP:11086998
631 631 c, t dbSNP:148342289
645 645 a, c dbSNP:774837924
652 652 a, g dbSNP:561338230
660 660 a, g dbSNP:768078284
667 667 c, t dbSNP:144466131
670 670 c, t dbSNP:147672904
671 671 g, t dbSNP:764257725
673 673 g, t dbSNP:754026379
678 678 c, t dbSNP:757516011
680 680 a, g dbSNP:779807906
693 693 c, t dbSNP:751437274
700 700 a, g dbSNP:376780996
701 701 a, g dbSNP:781200622
704 704 c, t dbSNP:199980487
705 705 a, g dbSNP:747561680
712 712 -, tt dbSNP:767094199
717 717 a, g dbSNP:769399729
721 721 a, c dbSNP:777475990
724 724 c, t dbSNP:748935911
727 727 a, g dbSNP:369693842
728 728 c, t dbSNP:774515822
729 729 c, t dbSNP:759961210
730 730 a, g dbSNP:150890139
733 733 c, t dbSNP:776099682
751 751 -, ag dbSNP:750063120
754 754 c, g dbSNP:761207472
759 759 a, g dbSNP:139300926
769 769 g, t dbSNP:753974727
800 800 -, g dbSNP:751626954
804 804 c, t dbSNP:762015042
812 812 c, t dbSNP:765500316
813 813 a, g dbSNP:529049263
834 834 c, t dbSNP:754823732
846 846 -, tgc dbSNP:755836869
849 849 c, t dbSNP:780950226
856 856 a, g dbSNP:752657781
863 863 c, t dbSNP:756178814
864 864 a, g dbSNP:777419143
881 881 c, g dbSNP:748815275
882 882 a, c dbSNP:770653224
884 884 c, t dbSNP:778653844
903 903 c, t dbSNP:746182207
904 904 a, g dbSNP:772447675
911 911 c, g dbSNP:775762947
912 912 c, t dbSNP:761154625
913 913 c, t dbSNP:769214684
915 915 a, c dbSNP:776624029
917 917 a, c, t dbSNP:761892578
919 919 c, g dbSNP:11569343
962 962 a, g dbSNP:565941222
971 971 g, t dbSNP:539494893
981 981 c, t dbSNP:369546762
1013 1013 a, g dbSNP:551789939
1048 1048 g, t dbSNP:570025940
1050 1050 g, t dbSNP:371282411
1062 1062 a, g dbSNP:537564344
1086 1086 c, g dbSNP:555863322
1090 1090 c, t dbSNP:772009573
1095 1095 c, t dbSNP:41282790
1100 1100 c, g dbSNP:11569344
1138 1138 a, g dbSNP:773341885
1156 1156 a, c dbSNP:117490488
1183 1183 a, g dbSNP:550037217
1242 1242 c, t dbSNP:747208252
1303 1303 c, t dbSNP:578190784
1331 1331 a, t dbSNP:184942163
1351 1351 g, t dbSNP:542031139

Target ORF information:

RefSeq Version XM_005260619
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGGTCCCCA GGATCGGCTG
AGAGCCCTGG TGGTGATCCC CATCATCTTC GGGATCCTGT TTGCCATCCT CTTGGTGCTG
GTCTTTATCA GTGAGTCCTC AGAAAAGGTG GCCAAGAAGC CAACCAATAA GGCCCCCCAC
CCCAAGCAGG AACCCCAGGA GATCAATTTT CCCGACGATC TTCCTGGCTC CAACACTGCT
GCTCCAGTGC AGGAGACTTT ACATGGATGC CAACCGGTCA CCCAGGAGGA TGGCAAAGAG
AGTCGCATCT CAGTGCAGGA GAGACAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56743
Accession Version XM_011529109.1
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTGTGAG ACCAAAGACC
TGGTTGTGCA ACAGGCAGGC ACAAACAAGA CTGATGTTGT CTGTGGTGAG TCCTGGACAA
TGGGCCCTGG AGAAAGCCTA G
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011527411.1
CDS95..595
Misc Feature(1)170..>499(+)
Misc Feature(2)278..403(+)
Translation
Position Chain Variation Link
2 2 c, t dbSNP:538304811
24 24 c, g dbSNP:556909578
26 26 -, t dbSNP:11569300
28 28 g, t dbSNP:575088069
34 34 g, t dbSNP:542745249
36 36 g, t dbSNP:554674186
40 40 c, g dbSNP:573086622
47 47 a, g dbSNP:746607340
48 48 c, g dbSNP:372855582
53 53 c, g dbSNP:768399532
54 54 c, t dbSNP:11569301
57 57 c, g dbSNP:762302983
58 58 c, g dbSNP:373167365
61 61 c, g dbSNP:773732954
62 62 c, t dbSNP:377285521
64 64 c, g dbSNP:766418920
65 65 a, t dbSNP:751711543
72 72 c, t dbSNP:755142543
75 75 c, g dbSNP:371166508
79 79 c, t dbSNP:753511518
82 82 g, t dbSNP:200981957
84 84 c, g dbSNP:778942629
92 92 a, g dbSNP:140399416
93 93 a, c dbSNP:758449676
94 94 c, t dbSNP:1883832
105 105 g, t dbSNP:746583774
114 114 a, c dbSNP:768187312
116 116 g, t dbSNP:113207193
122 122 a, c dbSNP:748416131
143 143 g, t dbSNP:770177808
148 148 c, g dbSNP:771363187
150 150 a, g dbSNP:774936391
159 159 c, t dbSNP:759483970
167 167 g, t dbSNP:147677886
169 169 a, g dbSNP:775472655
178 178 a, g dbSNP:760957101
189 189 c, t dbSNP:764464873
197 197 a, g dbSNP:750234130
198 198 a, g dbSNP:762843067
209 209 c, t dbSNP:766167612
217 217 c, t dbSNP:751549823
241 241 c, t dbSNP:541686651
250 250 a, g dbSNP:767422189
259 259 c, g, t dbSNP:752136889
265 265 a, g dbSNP:115724543
280 280 c, t dbSNP:753518969
281 281 a, g dbSNP:756898850
289 289 a, c dbSNP:202208745
295 295 c, t dbSNP:746276099
298 298 a, g dbSNP:142258778
301 301 a, c dbSNP:371950759
303 303 a, c dbSNP:747024806
324 324 g, t dbSNP:17855908
325 325 c, t dbSNP:768876770
328 328 a, c dbSNP:17177493
333 333 a, g dbSNP:376829285
341 341 c, t dbSNP:28931586
343 343 c, t dbSNP:776893342
346 346 c, t dbSNP:748382649
347 347 a, c dbSNP:769971386
353 353 c, g dbSNP:768637864
362 362 c, t dbSNP:144542285
363 363 a, g dbSNP:761229326
365 365 a, g dbSNP:764882779
366 366 c, t dbSNP:750063104
382 382 c, g, t dbSNP:763334863
385 385 a, g dbSNP:752031207
387 387 a, t dbSNP:755540822
406 406 c, t dbSNP:781766854
416 416 a, g dbSNP:752764084
419 419 c, t dbSNP:756171667
421 421 a, g dbSNP:778017892
423 423 a, g dbSNP:564200263
424 424 c, t dbSNP:771189629
429 429 a, c, t dbSNP:779810076
430 430 a, c, g dbSNP:267605960
438 438 a, c, t dbSNP:537489349
439 439 c, t dbSNP:147816161
440 440 c, t dbSNP:772829518
448 448 c, g dbSNP:555476733
452 452 g, t dbSNP:762684377
459 459 a, g dbSNP:766205073
462 462 a, g dbSNP:751910371
465 465 c, t dbSNP:11569321
469 469 c, t dbSNP:768090085
471 471 c, t dbSNP:753176464
472 472 a, g, t dbSNP:150312341
475 475 c, t dbSNP:763263083
476 476 a, g dbSNP:754006524
478 478 a, c dbSNP:757465117
484 484 g, t dbSNP:1801293
494 494 a, c, g dbSNP:61760052
495 495 c, t dbSNP:371997367
498 498 c, g dbSNP:144600981
500 500 a, g dbSNP:779766201
508 508 a, g dbSNP:747283803
516 516 c, g dbSNP:769096290
517 517 g, t dbSNP:777019036
530 530 a, c dbSNP:762283441
531 531 a, g dbSNP:374572404
553 553 a, g dbSNP:773211027
572 572 a, c dbSNP:763108539
595 595 c, g dbSNP:41282788
595 595 -, g dbSNP:756814674
603 603 g, t dbSNP:573008017
611 611 -, g dbSNP:781006748
656 656 c, g dbSNP:138900702
670 670 c, g dbSNP:757887786
699 699 c, t dbSNP:78467638
724 724 c, t dbSNP:577059887
812 812 a, g dbSNP:779575489
827 827 c, t dbSNP:149412480
860 860 g, t dbSNP:113827260
873 873 a, g dbSNP:574466055
874 874 c, t dbSNP:541936926
924 924 a, g dbSNP:754619036
957 957 c, t dbSNP:560368886
959 959 a, t dbSNP:781693104
1006 1006 a, t dbSNP:371865676
1013 1013 c, t dbSNP:549730603
1014 1014 a, g dbSNP:11548653

Target ORF information:

RefSeq Version XM_011529109
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529109

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTGTGAG ACCAAAGACC
TGGTTGTGCA ACAGGCAGGC ACAAACAAGA CTGATGTTGT CTGTGGTGAG TCCTGGACAA
TGGGCCCTGG AGAAAGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20663
Accession Version NM_001250.5
Sequence Information ORF Nucleotide Sequence (Length: 834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063089.1, BM761221.1, BC012419.1, BC064518.1 and BX364284.2. On Nov 14, 2014 this sequence version replaced gi:91105420. Summary: This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222896.1, BC012419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG
AAGCCAACCA ATAAGGCCCC CCACCCCAAG CAGGAACCCC AGGAGATCAA TTTTCCCGAC
GATCTTCCTG GCTCCAACAC TGCTGCTCCA GTGCAGGAGA CTTTACATGG ATGCCAACCG
GTCACCCAGG AGGATGGCAA AGAGAGTCGC ATCTCAGTGC AGGAGAGACA GTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001241.1
CDS91..924
Misc Feature(1)163..270(+)
Misc Feature(2)166..648(+)
Misc Feature(3)166..267(+)
Misc Feature(4)271..399(+)
Misc Feature(5)274..399(+)
Misc Feature(6)283..447(+)
Misc Feature(7)400..522(+)
Misc Feature(8)403..519(+)
Misc Feature(9)523..651(+)
Misc Feature(10)526..648(+)
Misc Feature(11)670..735(+)
Exon (1)1..141
Gene:CD40
Gene Synonym:
Exon (2)142..220
Gene:CD40
Gene Synonym:
Exon (3)221..346
Gene:CD40
Gene Synonym:
Exon (4)347..493
Gene:CD40
Gene Synonym:
Exon (5)494..587
Gene:CD40
Gene Synonym:
Exon (6)588..649
Gene:CD40
Gene Synonym:
Exon (7)650..736
Gene:CD40
Gene Synonym:
Exon (8)737..765
Gene:CD40
Gene Synonym:
Exon (9)766..1629
Gene:CD40
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, g dbSNP:556909578
22 22 -, t dbSNP:11569300
24 24 g, t dbSNP:575088069
30 30 g, t dbSNP:542745249
32 32 g, t dbSNP:554674186
36 36 c, g dbSNP:573086622
43 43 a, g dbSNP:746607340
44 44 c, g dbSNP:372855582
49 49 c, g dbSNP:768399532
50 50 c, t dbSNP:11569301
53 53 c, g dbSNP:762302983
54 54 c, g dbSNP:373167365
57 57 c, g dbSNP:773732954
58 58 c, t dbSNP:377285521
60 60 c, g dbSNP:766418920
61 61 a, t dbSNP:751711543
68 68 c, t dbSNP:755142543
71 71 c, g dbSNP:371166508
75 75 c, t dbSNP:753511518
78 78 g, t dbSNP:200981957
80 80 c, g dbSNP:778942629
88 88 a, g dbSNP:140399416
89 89 a, c dbSNP:758449676
90 90 c, t dbSNP:1883832
101 101 g, t dbSNP:746583774
110 110 a, c dbSNP:768187312
112 112 g, t dbSNP:113207193
118 118 a, c dbSNP:748416131
139 139 g, t dbSNP:770177808
144 144 c, g dbSNP:771363187
146 146 a, g dbSNP:774936391
155 155 c, t dbSNP:759483970
163 163 g, t dbSNP:147677886
165 165 a, g dbSNP:775472655
174 174 a, g dbSNP:760957101
185 185 c, t dbSNP:764464873
193 193 a, g dbSNP:750234130
194 194 a, g dbSNP:762843067
205 205 c, t dbSNP:766167612
213 213 c, t dbSNP:751549823
237 237 c, t dbSNP:541686651
246 246 a, g dbSNP:767422189
255 255 c, g, t dbSNP:752136889
261 261 a, g dbSNP:115724543
276 276 c, t dbSNP:753518969
277 277 a, g dbSNP:756898850
285 285 a, c dbSNP:202208745
291 291 c, t dbSNP:746276099
294 294 a, g dbSNP:142258778
297 297 a, c dbSNP:371950759
299 299 a, c dbSNP:747024806
320 320 g, t dbSNP:17855908
321 321 c, t dbSNP:768876770
324 324 a, c dbSNP:17177493
329 329 a, g dbSNP:376829285
337 337 c, t dbSNP:28931586
339 339 c, t dbSNP:776893342
342 342 c, t dbSNP:748382649
343 343 a, c dbSNP:769971386
349 349 c, g dbSNP:768637864
358 358 c, t dbSNP:144542285
359 359 a, g dbSNP:761229326
361 361 a, g dbSNP:764882779
362 362 c, t dbSNP:750063104
378 378 c, g, t dbSNP:763334863
381 381 a, g dbSNP:752031207
383 383 a, t dbSNP:755540822
402 402 c, t dbSNP:781766854
412 412 a, g dbSNP:752764084
415 415 c, t dbSNP:756171667
417 417 a, g dbSNP:778017892
419 419 a, g dbSNP:564200263
420 420 c, t dbSNP:771189629
425 425 a, c, t dbSNP:779810076
426 426 a, c, g dbSNP:267605960
434 434 a, c, t dbSNP:537489349
435 435 c, t dbSNP:147816161
436 436 c, t dbSNP:772829518
444 444 c, g dbSNP:555476733
448 448 g, t dbSNP:762684377
455 455 a, g dbSNP:766205073
458 458 a, g dbSNP:751910371
461 461 c, t dbSNP:11569321
465 465 c, t dbSNP:768090085
467 467 c, t dbSNP:753176464
468 468 a, g, t dbSNP:150312341
471 471 c, t dbSNP:763263083
472 472 a, g dbSNP:754006524
474 474 a, c dbSNP:757465117
480 480 g, t dbSNP:1801293
490 490 a, c, g dbSNP:61760052
491 491 c, t dbSNP:371997367
502 502 g, t dbSNP:368921167
503 503 c, t dbSNP:755938402
513 513 c, t dbSNP:777779940
519 519 c, t dbSNP:373653555
520 520 a, g dbSNP:770600305
525 525 c, t dbSNP:376668410
534 534 c, t dbSNP:745496687
537 537 c, t dbSNP:771812128
548 548 a, g, t dbSNP:745551504
555 555 a, g dbSNP:573253733
558 558 c, t dbSNP:764447217
559 559 a, g dbSNP:777145547
564 564 a, c, t dbSNP:79661585
588 588 c, g dbSNP:144600981
590 590 a, g dbSNP:779766201
598 598 a, g dbSNP:747283803
606 606 c, g dbSNP:769096290
607 607 g, t dbSNP:777019036
620 620 a, c dbSNP:762283441
621 621 a, g dbSNP:374572404
643 643 a, g dbSNP:773211027
652 652 c, t dbSNP:757943526
653 653 a, c, t dbSNP:779532941
657 657 a, g dbSNP:768240688
662 662 a, g dbSNP:781521147
675 675 c, g dbSNP:748519791
690 690 c, g dbSNP:537635771
696 696 c, t dbSNP:7273698
700 700 a, g dbSNP:143037975
709 709 a, g dbSNP:762902889
711 711 c, t dbSNP:535886332
712 712 a, g dbSNP:199581355
713 713 c, t dbSNP:759793763
715 715 c, t dbSNP:767920606
723 723 a, g dbSNP:776500140
743 743 g, t dbSNP:766292495
745 745 a, g dbSNP:368619894
749 749 a, g dbSNP:371799172
750 750 a, g dbSNP:754507112
753 753 a, g dbSNP:371353981
762 762 g, t dbSNP:767118191
767 767 c, t dbSNP:773019543
769 769 c, g, t dbSNP:11086998
771 771 c, t dbSNP:148342289
785 785 a, c dbSNP:774837924
792 792 a, g dbSNP:561338230
800 800 a, g dbSNP:768078284
807 807 c, t dbSNP:144466131
810 810 c, t dbSNP:147672904
811 811 g, t dbSNP:764257725
813 813 g, t dbSNP:754026379
818 818 c, t dbSNP:757516011
820 820 a, g dbSNP:779807906
833 833 c, t dbSNP:751437274
840 840 a, g dbSNP:376780996
841 841 a, g dbSNP:781200622
844 844 c, t dbSNP:199980487
845 845 a, g dbSNP:747561680
852 852 -, tt dbSNP:767094199
857 857 a, g dbSNP:769399729
861 861 a, c dbSNP:777475990
864 864 c, t dbSNP:748935911
867 867 a, g dbSNP:369693842
868 868 c, t dbSNP:774515822
869 869 c, t dbSNP:759961210
870 870 a, g dbSNP:150890139
873 873 c, t dbSNP:776099682
891 891 -, ag dbSNP:750063120
894 894 c, g dbSNP:761207472
899 899 a, g dbSNP:139300926
909 909 g, t dbSNP:753974727
940 940 -, g dbSNP:751626954
944 944 c, t dbSNP:762015042
952 952 c, t dbSNP:765500316
953 953 a, g dbSNP:529049263
974 974 c, t dbSNP:754823732
986 986 -, tgc dbSNP:755836869
989 989 c, t dbSNP:780950226
996 996 a, g dbSNP:752657781
1003 1003 c, t dbSNP:756178814
1004 1004 a, g dbSNP:777419143
1021 1021 c, g dbSNP:748815275
1022 1022 a, c dbSNP:770653224
1024 1024 c, t dbSNP:778653844
1043 1043 c, t dbSNP:746182207
1044 1044 a, g dbSNP:772447675
1051 1051 c, g dbSNP:775762947
1052 1052 c, t dbSNP:761154625
1053 1053 c, t dbSNP:769214684
1055 1055 a, c dbSNP:776624029
1057 1057 a, c, t dbSNP:761892578
1059 1059 c, g dbSNP:11569343
1102 1102 a, g dbSNP:565941222
1111 1111 g, t dbSNP:539494893
1121 1121 c, t dbSNP:369546762
1153 1153 a, g dbSNP:551789939
1188 1188 g, t dbSNP:570025940
1190 1190 g, t dbSNP:371282411
1202 1202 a, g dbSNP:537564344
1226 1226 c, g dbSNP:555863322
1230 1230 c, t dbSNP:772009573
1235 1235 c, t dbSNP:41282790
1240 1240 c, g dbSNP:11569344
1278 1278 a, g dbSNP:773341885
1296 1296 a, c dbSNP:117490488
1323 1323 a, g dbSNP:550037217
1382 1382 c, t dbSNP:747208252
1443 1443 c, t dbSNP:578190784
1471 1471 a, t dbSNP:184942163
1491 1491 g, t dbSNP:542031139
1504 1504 c, t dbSNP:6094253
1537 1537 c, t dbSNP:41282792
1587 1587 c, t dbSNP:565280552
1601 1601 a, g dbSNP:370599881

Target ORF information:

RefSeq Version NM_001250
Organism Homo sapiens (human)
Definition Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001250

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG
AAGCCAACCA ATAAGGCCCC CCACCCCAAG CAGGAACCCC AGGAGATCAA TTTTCCCGAC
GATCTTCCTG GCTCCAACAC TGCTGCTCCA GTGCAGGAGA CTTTACATGG ATGCCAACCG
GTCACCCAGG AGGATGGCAA AGAGAGTCGC ATCTCAGTGC AGGAGAGACA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53156
Accession Version NM_001302753.1
Sequence Information ORF Nucleotide Sequence (Length: 714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063089.1, BM761221.1, BC012419.1, BX364285.2, BC064518.1 and BX364284.2. On Nov 14, 2014 this sequence version replaced gi:530418534. Summary: This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX364285.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TGAGTCCTGG ACAATGGGCC CTGGAGAAAG CCTAGGAAGG
TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC ATCTTCGGGA TCCTGTTTGC
CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG AAGCCAACCA ATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001289682.1
CDS91..804
Misc Feature(1)163..270(+)
Misc Feature(2)166..648(+)
Misc Feature(3)166..267(+)
Misc Feature(4)271..399(+)
Misc Feature(5)274..399(+)
Misc Feature(6)283..447(+)
Misc Feature(7)400..522(+)
Misc Feature(8)403..519(+)
Misc Feature(9)523..651(+)
Misc Feature(10)526..648(+)
Exon (1)1..141
Gene:CD40
Gene Synonym:
Exon (2)142..220
Gene:CD40
Gene Synonym:
Exon (3)221..346
Gene:CD40
Gene Synonym:
Exon (4)347..493
Gene:CD40
Gene Synonym:
Exon (5)494..587
Gene:CD40
Gene Synonym:
Exon (6)588..689
Gene:CD40
Gene Synonym:
Exon (7)690..776
Gene:CD40
Gene Synonym:
Exon (8)777..805
Gene:CD40
Gene Synonym:
Exon (9)806..1669
Gene:CD40
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, g dbSNP:556909578
22 22 -, t dbSNP:11569300
24 24 g, t dbSNP:575088069
30 30 g, t dbSNP:542745249
32 32 g, t dbSNP:554674186
36 36 c, g dbSNP:573086622
43 43 a, g dbSNP:746607340
44 44 c, g dbSNP:372855582
49 49 c, g dbSNP:768399532
50 50 c, t dbSNP:11569301
53 53 c, g dbSNP:762302983
54 54 c, g dbSNP:373167365
57 57 c, g dbSNP:773732954
58 58 c, t dbSNP:377285521
60 60 c, g dbSNP:766418920
61 61 a, t dbSNP:751711543
68 68 c, t dbSNP:755142543
71 71 c, g dbSNP:371166508
75 75 c, t dbSNP:753511518
78 78 g, t dbSNP:200981957
80 80 c, g dbSNP:778942629
88 88 a, g dbSNP:140399416
89 89 a, c dbSNP:758449676
90 90 c, t dbSNP:1883832
101 101 g, t dbSNP:746583774
110 110 a, c dbSNP:768187312
112 112 g, t dbSNP:113207193
118 118 a, c dbSNP:748416131
139 139 g, t dbSNP:770177808
144 144 c, g dbSNP:771363187
146 146 a, g dbSNP:774936391
155 155 c, t dbSNP:759483970
163 163 g, t dbSNP:147677886
165 165 a, g dbSNP:775472655
174 174 a, g dbSNP:760957101
185 185 c, t dbSNP:764464873
193 193 a, g dbSNP:750234130
194 194 a, g dbSNP:762843067
205 205 c, t dbSNP:766167612
213 213 c, t dbSNP:751549823
237 237 c, t dbSNP:541686651
246 246 a, g dbSNP:767422189
255 255 c, g, t dbSNP:752136889
261 261 a, g dbSNP:115724543
276 276 c, t dbSNP:753518969
277 277 a, g dbSNP:756898850
285 285 a, c dbSNP:202208745
291 291 c, t dbSNP:746276099
294 294 a, g dbSNP:142258778
297 297 a, c dbSNP:371950759
299 299 a, c dbSNP:747024806
320 320 g, t dbSNP:17855908
321 321 c, t dbSNP:768876770
324 324 a, c dbSNP:17177493
329 329 a, g dbSNP:376829285
337 337 c, t dbSNP:28931586
339 339 c, t dbSNP:776893342
342 342 c, t dbSNP:748382649
343 343 a, c dbSNP:769971386
349 349 c, g dbSNP:768637864
358 358 c, t dbSNP:144542285
359 359 a, g dbSNP:761229326
361 361 a, g dbSNP:764882779
362 362 c, t dbSNP:750063104
378 378 c, g, t dbSNP:763334863
381 381 a, g dbSNP:752031207
383 383 a, t dbSNP:755540822
402 402 c, t dbSNP:781766854
412 412 a, g dbSNP:752764084
415 415 c, t dbSNP:756171667
417 417 a, g dbSNP:778017892
419 419 a, g dbSNP:564200263
420 420 c, t dbSNP:771189629
425 425 a, c, t dbSNP:779810076
426 426 a, c, g dbSNP:267605960
434 434 a, c, t dbSNP:537489349
435 435 c, t dbSNP:147816161
436 436 c, t dbSNP:772829518
444 444 c, g dbSNP:555476733
448 448 g, t dbSNP:762684377
455 455 a, g dbSNP:766205073
458 458 a, g dbSNP:751910371
461 461 c, t dbSNP:11569321
465 465 c, t dbSNP:768090085
467 467 c, t dbSNP:753176464
468 468 a, g, t dbSNP:150312341
471 471 c, t dbSNP:763263083
472 472 a, g dbSNP:754006524
474 474 a, c dbSNP:757465117
480 480 g, t dbSNP:1801293
490 490 a, c, g dbSNP:61760052
491 491 c, t dbSNP:371997367
502 502 g, t dbSNP:368921167
503 503 c, t dbSNP:755938402
513 513 c, t dbSNP:777779940
519 519 c, t dbSNP:373653555
520 520 a, g dbSNP:770600305
525 525 c, t dbSNP:376668410
534 534 c, t dbSNP:745496687
537 537 c, t dbSNP:771812128
548 548 a, g, t dbSNP:745551504
555 555 a, g dbSNP:573253733
558 558 c, t dbSNP:764447217
559 559 a, g dbSNP:777145547
564 564 a, c, t dbSNP:79661585
588 588 c, g dbSNP:144600981
590 590 a, g dbSNP:779766201
598 598 a, g dbSNP:747283803
606 606 c, g dbSNP:769096290
607 607 g, t dbSNP:777019036
620 620 a, c dbSNP:762283441
621 621 a, g dbSNP:374572404
643 643 a, g dbSNP:773211027
662 662 a, c dbSNP:763108539
685 685 c, g dbSNP:41282788
685 685 -, g dbSNP:756814674
692 692 c, t dbSNP:757943526
693 693 a, c, t dbSNP:779532941
697 697 a, g dbSNP:768240688
702 702 a, g dbSNP:781521147
715 715 c, g dbSNP:748519791
730 730 c, g dbSNP:537635771
736 736 c, t dbSNP:7273698
740 740 a, g dbSNP:143037975
749 749 a, g dbSNP:762902889
751 751 c, t dbSNP:535886332
752 752 a, g dbSNP:199581355
753 753 c, t dbSNP:759793763
755 755 c, t dbSNP:767920606
763 763 a, g dbSNP:776500140
783 783 g, t dbSNP:766292495
785 785 a, g dbSNP:368619894
789 789 a, g dbSNP:371799172
790 790 a, g dbSNP:754507112
793 793 a, g dbSNP:371353981
802 802 g, t dbSNP:767118191
807 807 c, t dbSNP:773019543
809 809 c, g, t dbSNP:11086998
811 811 c, t dbSNP:148342289
825 825 a, c dbSNP:774837924
832 832 a, g dbSNP:561338230
840 840 a, g dbSNP:768078284
847 847 c, t dbSNP:144466131
850 850 c, t dbSNP:147672904
851 851 g, t dbSNP:764257725
853 853 g, t dbSNP:754026379
858 858 c, t dbSNP:757516011
860 860 a, g dbSNP:779807906
873 873 c, t dbSNP:751437274
880 880 a, g dbSNP:376780996
881 881 a, g dbSNP:781200622
884 884 c, t dbSNP:199980487
885 885 a, g dbSNP:747561680
892 892 -, tt dbSNP:767094199
897 897 a, g dbSNP:769399729
901 901 a, c dbSNP:777475990
904 904 c, t dbSNP:748935911
907 907 a, g dbSNP:369693842
908 908 c, t dbSNP:774515822
909 909 c, t dbSNP:759961210
910 910 a, g dbSNP:150890139
913 913 c, t dbSNP:776099682
931 931 -, ag dbSNP:750063120
934 934 c, g dbSNP:761207472
939 939 a, g dbSNP:139300926
949 949 g, t dbSNP:753974727
980 980 -, g dbSNP:751626954
984 984 c, t dbSNP:762015042
992 992 c, t dbSNP:765500316
993 993 a, g dbSNP:529049263
1014 1014 c, t dbSNP:754823732
1026 1026 -, tgc dbSNP:755836869
1029 1029 c, t dbSNP:780950226
1036 1036 a, g dbSNP:752657781
1043 1043 c, t dbSNP:756178814
1044 1044 a, g dbSNP:777419143
1061 1061 c, g dbSNP:748815275
1062 1062 a, c dbSNP:770653224
1064 1064 c, t dbSNP:778653844
1083 1083 c, t dbSNP:746182207
1084 1084 a, g dbSNP:772447675
1091 1091 c, g dbSNP:775762947
1092 1092 c, t dbSNP:761154625
1093 1093 c, t dbSNP:769214684
1095 1095 a, c dbSNP:776624029
1097 1097 a, c, t dbSNP:761892578
1099 1099 c, g dbSNP:11569343
1142 1142 a, g dbSNP:565941222
1151 1151 g, t dbSNP:539494893
1161 1161 c, t dbSNP:369546762
1193 1193 a, g dbSNP:551789939
1228 1228 g, t dbSNP:570025940
1230 1230 g, t dbSNP:371282411
1242 1242 a, g dbSNP:537564344
1266 1266 c, g dbSNP:555863322
1270 1270 c, t dbSNP:772009573
1275 1275 c, t dbSNP:41282790
1280 1280 c, g dbSNP:11569344
1318 1318 a, g dbSNP:773341885
1336 1336 a, c dbSNP:117490488
1363 1363 a, g dbSNP:550037217
1422 1422 c, t dbSNP:747208252
1483 1483 c, t dbSNP:578190784
1511 1511 a, t dbSNP:184942163
1531 1531 g, t dbSNP:542031139
1544 1544 c, t dbSNP:6094253
1577 1577 c, t dbSNP:41282792
1627 1627 c, t dbSNP:565280552
1641 1641 a, g dbSNP:370599881

Target ORF information:

RefSeq Version NM_001302753
Organism Homo sapiens (human)
Definition Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302753

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TGAGTCCTGG ACAATGGGCC CTGGAGAAAG CCTAGGAAGG
TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC ATCTTCGGGA TCCTGTTTGC
CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG AAGCCAACCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12389
Accession Version NM_152854.3
Sequence Information ORF Nucleotide Sequence (Length: 612bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063089.1, BM761221.1, BC012419.1, BC064518.1 and BX364284.2. On Nov 14, 2014 this sequence version replaced gi:91105277. Summary: This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) contains a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ300189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGGTC CCCAGGATCG GCTGAGAGCC CTGGTGGTGA TCCCCATCAT
CTTCGGGATC CTGTTTGCCA TCCTCTTGGT GCTGGTCTTT ATCAAAAAGG TGGCCAAGAA
GCCAACCAAT AA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_690593.1
CDS91..702
Misc Feature(1)163..270(+)
Misc Feature(2)166..588(+)
Misc Feature(3)166..267(+)
Misc Feature(4)271..399(+)
Misc Feature(5)274..399(+)
Misc Feature(6)283..447(+)
Misc Feature(7)400..522(+)
Misc Feature(8)403..519(+)
Misc Feature(9)526..588(+)
Exon (1)1..141
Gene:CD40
Gene Synonym:
Exon (2)142..220
Gene:CD40
Gene Synonym:
Exon (3)221..346
Gene:CD40
Gene Synonym:
Exon (4)347..493
Gene:CD40
Gene Synonym:
Exon (5)494..587
Gene:CD40
Gene Synonym:
Exon (6)588..674
Gene:CD40
Gene Synonym:
Exon (7)675..703
Gene:CD40
Gene Synonym:
Exon (8)704..1567
Gene:CD40
Gene Synonym:
Translation
Position Chain Variation Link
20 20 c, g dbSNP:556909578
22 22 -, t dbSNP:11569300
24 24 g, t dbSNP:575088069
30 30 g, t dbSNP:542745249
32 32 g, t dbSNP:554674186
36 36 c, g dbSNP:573086622
43 43 a, g dbSNP:746607340
44 44 c, g dbSNP:372855582
49 49 c, g dbSNP:768399532
50 50 c, t dbSNP:11569301
53 53 c, g dbSNP:762302983
54 54 c, g dbSNP:373167365
57 57 c, g dbSNP:773732954
58 58 c, t dbSNP:377285521
60 60 c, g dbSNP:766418920
61 61 a, t dbSNP:751711543
68 68 c, t dbSNP:755142543
71 71 c, g dbSNP:371166508
75 75 c, t dbSNP:753511518
78 78 g, t dbSNP:200981957
80 80 c, g dbSNP:778942629
88 88 a, g dbSNP:140399416
89 89 a, c dbSNP:758449676
90 90 c, t dbSNP:1883832
101 101 g, t dbSNP:746583774
110 110 a, c dbSNP:768187312
112 112 g, t dbSNP:113207193
118 118 a, c dbSNP:748416131
139 139 g, t dbSNP:770177808
144 144 c, g dbSNP:771363187
146 146 a, g dbSNP:774936391
155 155 c, t dbSNP:759483970
163 163 g, t dbSNP:147677886
165 165 a, g dbSNP:775472655
174 174 a, g dbSNP:760957101
185 185 c, t dbSNP:764464873
193 193 a, g dbSNP:750234130
194 194 a, g dbSNP:762843067
205 205 c, t dbSNP:766167612
213 213 c, t dbSNP:751549823
237 237 c, t dbSNP:541686651
246 246 a, g dbSNP:767422189
255 255 c, g, t dbSNP:752136889
261 261 a, g dbSNP:115724543
276 276 c, t dbSNP:753518969
277 277 a, g dbSNP:756898850
285 285 a, c dbSNP:202208745
291 291 c, t dbSNP:746276099
294 294 a, g dbSNP:142258778
297 297 a, c dbSNP:371950759
299 299 a, c dbSNP:747024806
320 320 g, t dbSNP:17855908
321 321 c, t dbSNP:768876770
324 324 a, c dbSNP:17177493
329 329 a, g dbSNP:376829285
337 337 c, t dbSNP:28931586
339 339 c, t dbSNP:776893342
342 342 c, t dbSNP:748382649
343 343 a, c dbSNP:769971386
349 349 c, g dbSNP:768637864
358 358 c, t dbSNP:144542285
359 359 a, g dbSNP:761229326
361 361 a, g dbSNP:764882779
362 362 c, t dbSNP:750063104
378 378 c, g, t dbSNP:763334863
381 381 a, g dbSNP:752031207
383 383 a, t dbSNP:755540822
402 402 c, t dbSNP:781766854
412 412 a, g dbSNP:752764084
415 415 c, t dbSNP:756171667
417 417 a, g dbSNP:778017892
419 419 a, g dbSNP:564200263
420 420 c, t dbSNP:771189629
425 425 a, c, t dbSNP:779810076
426 426 a, c, g dbSNP:267605960
434 434 a, c, t dbSNP:537489349
435 435 c, t dbSNP:147816161
436 436 c, t dbSNP:772829518
444 444 c, g dbSNP:555476733
448 448 g, t dbSNP:762684377
455 455 a, g dbSNP:766205073
458 458 a, g dbSNP:751910371
461 461 c, t dbSNP:11569321
465 465 c, t dbSNP:768090085
467 467 c, t dbSNP:753176464
468 468 a, g, t dbSNP:150312341
471 471 c, t dbSNP:763263083
472 472 a, g dbSNP:754006524
474 474 a, c dbSNP:757465117
480 480 g, t dbSNP:1801293
490 490 a, c, g dbSNP:61760052
491 491 c, t dbSNP:371997367
502 502 g, t dbSNP:368921167
503 503 c, t dbSNP:755938402
513 513 c, t dbSNP:777779940
519 519 c, t dbSNP:373653555
520 520 a, g dbSNP:770600305
525 525 c, t dbSNP:376668410
534 534 c, t dbSNP:745496687
537 537 c, t dbSNP:771812128
548 548 a, g, t dbSNP:745551504
555 555 a, g dbSNP:573253733
558 558 c, t dbSNP:764447217
559 559 a, g dbSNP:777145547
564 564 a, c, t dbSNP:79661585
590 590 c, t dbSNP:757943526
591 591 a, c, t dbSNP:779532941
595 595 a, g dbSNP:768240688
600 600 a, g dbSNP:781521147
613 613 c, g dbSNP:748519791
628 628 c, g dbSNP:537635771
634 634 c, t dbSNP:7273698
638 638 a, g dbSNP:143037975
647 647 a, g dbSNP:762902889
649 649 c, t dbSNP:535886332
650 650 a, g dbSNP:199581355
651 651 c, t dbSNP:759793763
653 653 c, t dbSNP:767920606
661 661 a, g dbSNP:776500140
681 681 g, t dbSNP:766292495
683 683 a, g dbSNP:368619894
687 687 a, g dbSNP:371799172
688 688 a, g dbSNP:754507112
691 691 a, g dbSNP:371353981
700 700 g, t dbSNP:767118191
705 705 c, t dbSNP:773019543
707 707 c, g, t dbSNP:11086998
709 709 c, t dbSNP:148342289
723 723 a, c dbSNP:774837924
730 730 a, g dbSNP:561338230
738 738 a, g dbSNP:768078284
745 745 c, t dbSNP:144466131
748 748 c, t dbSNP:147672904
749 749 g, t dbSNP:764257725
751 751 g, t dbSNP:754026379
756 756 c, t dbSNP:757516011
758 758 a, g dbSNP:779807906
771 771 c, t dbSNP:751437274
778 778 a, g dbSNP:376780996
779 779 a, g dbSNP:781200622
782 782 c, t dbSNP:199980487
783 783 a, g dbSNP:747561680
790 790 -, tt dbSNP:767094199
795 795 a, g dbSNP:769399729
799 799 a, c dbSNP:777475990
802 802 c, t dbSNP:748935911
805 805 a, g dbSNP:369693842
806 806 c, t dbSNP:774515822
807 807 c, t dbSNP:759961210
808 808 a, g dbSNP:150890139
811 811 c, t dbSNP:776099682
829 829 -, ag dbSNP:750063120
832 832 c, g dbSNP:761207472
837 837 a, g dbSNP:139300926
847 847 g, t dbSNP:753974727
878 878 -, g dbSNP:751626954
882 882 c, t dbSNP:762015042
890 890 c, t dbSNP:765500316
891 891 a, g dbSNP:529049263
912 912 c, t dbSNP:754823732
924 924 -, tgc dbSNP:755836869
927 927 c, t dbSNP:780950226
934 934 a, g dbSNP:752657781
941 941 c, t dbSNP:756178814
942 942 a, g dbSNP:777419143
959 959 c, g dbSNP:748815275
960 960 a, c dbSNP:770653224
962 962 c, t dbSNP:778653844
981 981 c, t dbSNP:746182207
982 982 a, g dbSNP:772447675
989 989 c, g dbSNP:775762947
990 990 c, t dbSNP:761154625
991 991 c, t dbSNP:769214684
993 993 a, c dbSNP:776624029
995 995 a, c, t dbSNP:761892578
997 997 c, g dbSNP:11569343
1040 1040 a, g dbSNP:565941222
1049 1049 g, t dbSNP:539494893
1059 1059 c, t dbSNP:369546762
1091 1091 a, g dbSNP:551789939
1126 1126 g, t dbSNP:570025940
1128 1128 g, t dbSNP:371282411
1140 1140 a, g dbSNP:537564344
1164 1164 c, g dbSNP:555863322
1168 1168 c, t dbSNP:772009573
1173 1173 c, t dbSNP:41282790
1178 1178 c, g dbSNP:11569344
1216 1216 a, g dbSNP:773341885
1234 1234 a, c dbSNP:117490488
1261 1261 a, g dbSNP:550037217
1320 1320 c, t dbSNP:747208252
1381 1381 c, t dbSNP:578190784
1409 1409 a, t dbSNP:184942163
1429 1429 g, t dbSNP:542031139
1442 1442 c, t dbSNP:6094253
1475 1475 c, t dbSNP:41282792
1525 1525 c, t dbSNP:565280552
1539 1539 a, g dbSNP:370599881

Target ORF information:

RefSeq Version NM_152854
Organism Homo sapiens (human)
Definition Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152854

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGGTC CCCAGGATCG GCTGAGAGCC CTGGTGGTGA TCCCCATCAT
CTTCGGGATC CTGTTTGCCA TCCTCTTGGT GCTGGTCTTT ATCAAAAAGG TGGCCAAGAA
GCCAACCAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.