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CD40 cDNA ORF clone, Homo sapiens (human)

Gene Symbol CD40
Entrez Gene ID 958
Full Name CD40 molecule, TNF receptor superfamily member 5
Synonyms Bp50, CDW40, TNFRSF5, p50
General protein information
Preferred Names
tumor necrosis factor receptor superfamily member 5
Names
tumor necrosis factor receptor superfamily member 5
CD40L receptor
CD40 type II isoform
B cell-associated molecule
B cell surface antigen CD40
B-cell surface antigen CD40
CD40 antigen (TNF receptor superfamily member 5)
tumor necrosis factor receptor superfamily, member 5
nerve growth factor receptor-related B-lymphocyte activation molecule
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

20

20q12-q13.2

Summary This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]. lac of sum
Disorder MIM:

109535

Disorder Html: Immunodeficiency with hyper-IgM, type 3, 606843 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005260617 XP_005260674 tumor necrosis factor receptor superfamily member 5 isoform X1
XM_005260619 XP_005260676 tumor necrosis factor receptor superfamily member 5 isoform X2
XM_011529109 XP_011527411 tumor necrosis factor receptor superfamily member 5 isoform X3
NM_001250 NP_001241 tumor necrosis factor receptor superfamily member 5 isoform 1 precursor
NM_001302753 NP_001289682 tumor necrosis factor receptor superfamily member 5 isoform 3 precursor
NM_152854 NP_690593 tumor necrosis factor receptor superfamily member 5 isoform 2 precursor



Homo sapiens (human) CD40 NP_001241.1
Pan troglodytes (chimpanzee) CD40 XP_003317038.2
Macaca mulatta (Rhesus monkey) CD40 XP_001104333.1
Canis lupus familiaris (dog) CD40 NP_001002982.1
Bos taurus (cattle) CD40 NP_001099081.1
Mus musculus (house mouse) Cd40 NP_035741.2
Rattus norvegicus (Norway rat) Cd40 NP_599187.1
Gallus gallus (chicken) CD40 NP_989996.1
Xenopus (Silurana) tropicalis (western clawed frog) cd40 NP_001138688.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CD40 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD40 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu53155 XM_005260617 PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu53158 XM_005260619 PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu56743 XM_011529109 PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu20663
NM_001250 Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu53156 NM_001302753 Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu12389
NM_152854 Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu53155
Clone ID Related Accession (Same CDS sequence) XM_005260617
Accession Version XM_005260617.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530418532. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAGTGA GTCCTCAGAA
AAGGTGGCCA AGAAGCCAAC CAATAAGGCC CCCCACCCCA AGCAGGAACC CCAGGAGATC
AATTTTCCCG ACGATCTTCC TGGCTCCAAC ACTGCTGCTC CAGTGCAGGA GACTTTACAT
GGATGCCAAC CGGTCACCCA GGAGGATGGC AAAGAGAGTC GCATCTCAGT GCAGGAGAGA
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260674.1
CDS94..939
Misc Feature(1)169..651(+)
Misc Feature(2)169..270(+)
Misc Feature(3)277..402(+)
Misc Feature(4)286..450(+)
Misc Feature(5)406..522(+)
Misc Feature(6)529..651(+)
Translation

Target ORF information:

RefSeq Version XM_005260617
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260617

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAGTGA GTCCTCAGAA
AAGGTGGCCA AGAAGCCAAC CAATAAGGCC CCCCACCCCA AGCAGGAACC CCAGGAGATC
AATTTTCCCG ACGATCTTCC TGGCTCCAAC ACTGCTGCTC CAGTGCAGGA GACTTTACAT
GGATGCCAAC CGGTCACCCA GGAGGATGGC AAAGAGAGTC GCATCTCAGT GCAGGAGAGA
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53158
Clone ID Related Accession (Same CDS sequence) XM_005260619
Accession Version XM_005260619.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530418536. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGGTCCCCA GGATCGGCTG
AGAGCCCTGG TGGTGATCCC CATCATCTTC GGGATCCTGT TTGCCATCCT CTTGGTGCTG
GTCTTTATCA GTGAGTCCTC AGAAAAGGTG GCCAAGAAGC CAACCAATAA GGCCCCCCAC
CCCAAGCAGG AACCCCAGGA GATCAATTTT CCCGACGATC TTCCTGGCTC CAACACTGCT
GCTCCAGTGC AGGAGACTTT ACATGGATGC CAACCGGTCA CCCAGGAGGA TGGCAAAGAG
AGTCGCATCT CAGTGCAGGA GAGACAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005260676.1
CDS95..784
Misc Feature(1)170..>499(+)
Misc Feature(2)278..403(+)
Translation

Target ORF information:

RefSeq Version XM_005260619
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005260619

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGGTCCCCA GGATCGGCTG
AGAGCCCTGG TGGTGATCCC CATCATCTTC GGGATCCTGT TTGCCATCCT CTTGGTGCTG
GTCTTTATCA GTGAGTCCTC AGAAAAGGTG GCCAAGAAGC CAACCAATAA GGCCCCCCAC
CCCAAGCAGG AACCCCAGGA GATCAATTTT CCCGACGATC TTCCTGGCTC CAACACTGCT
GCTCCAGTGC AGGAGACTTT ACATGGATGC CAACCGGTCA CCCAGGAGGA TGGCAAAGAG
AGTCGCATCT CAGTGCAGGA GAGACAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56743
Clone ID Related Accession (Same CDS sequence) XM_011529109
Accession Version XM_011529109.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011362.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTGTGAG ACCAAAGACC
TGGTTGTGCA ACAGGCAGGC ACAAACAAGA CTGATGTTGT CTGTGGTGAG TCCTGGACAA
TGGGCCCTGG AGAAAGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527411.1
CDS95..595
Misc Feature(1)170..>499(+)
Misc Feature(2)278..403(+)
Translation

Target ORF information:

RefSeq Version XM_011529109
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011529109

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTGTGAG ACCAAAGACC
TGGTTGTGCA ACAGGCAGGC ACAAACAAGA CTGATGTTGT CTGTGGTGAG TCCTGGACAA
TGGGCCCTGG AGAAAGCCTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20663
Clone ID Related Accession (Same CDS sequence) NM_001250
Accession Version NM_001250.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063089.1, BM761221.1, BC012419.1, BC064518.1 and BX364284.2. On Nov 14, 2014 this sequence version replaced gi:91105420. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222896.1, BC012419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG
AAGCCAACCA ATAAGGCCCC CCACCCCAAG CAGGAACCCC AGGAGATCAA TTTTCCCGAC
GATCTTCCTG GCTCCAACAC TGCTGCTCCA GTGCAGGAGA CTTTACATGG ATGCCAACCG
GTCACCCAGG AGGATGGCAA AGAGAGTCGC ATCTCAGTGC AGGAGAGACA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001241.1
CDS91..924
Misc Feature(1)163..270(+)
Misc Feature(2)166..648(+)
Misc Feature(3)166..267(+)
Misc Feature(4)271..399(+)
Misc Feature(5)274..399(+)
Misc Feature(6)283..447(+)
Misc Feature(7)400..522(+)
Misc Feature(8)403..519(+)
Misc Feature(9)523..651(+)
Misc Feature(10)526..648(+)
Misc Feature(11)670..735(+)
Exon (1)1..141
Gene:CD40
Gene Synonym:
Exon (2)142..220
Gene:CD40
Gene Synonym:
Exon (3)221..346
Gene:CD40
Gene Synonym:
Exon (4)347..493
Gene:CD40
Gene Synonym:
Exon (5)494..587
Gene:CD40
Gene Synonym:
Exon (6)588..649
Gene:CD40
Gene Synonym:
Exon (7)650..736
Gene:CD40
Gene Synonym:
Exon (8)737..765
Gene:CD40
Gene Synonym:
Exon (9)766..1629
Gene:CD40
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001250
Organism Homo sapiens (human)
Definition Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001250

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC
ATCTTCGGGA TCCTGTTTGC CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG
AAGCCAACCA ATAAGGCCCC CCACCCCAAG CAGGAACCCC AGGAGATCAA TTTTCCCGAC
GATCTTCCTG GCTCCAACAC TGCTGCTCCA GTGCAGGAGA CTTTACATGG ATGCCAACCG
GTCACCCAGG AGGATGGCAA AGAGAGTCGC ATCTCAGTGC AGGAGAGACA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu53156
Clone ID Related Accession (Same CDS sequence) NM_001302753
Accession Version NM_001302753.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063089.1, BM761221.1, BC012419.1, BX364285.2, BC064518.1 and BX364284.2. On Nov 14, 2014 this sequence version replaced gi:530418534. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX364285.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TGAGTCCTGG ACAATGGGCC CTGGAGAAAG CCTAGGAAGG
TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC ATCTTCGGGA TCCTGTTTGC
CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG AAGCCAACCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289682.1
CDS91..804
Misc Feature(1)163..270(+)
Misc Feature(2)166..648(+)
Misc Feature(3)166..267(+)
Misc Feature(4)271..399(+)
Misc Feature(5)274..399(+)
Misc Feature(6)283..447(+)
Misc Feature(7)400..522(+)
Misc Feature(8)403..519(+)
Misc Feature(9)523..651(+)
Misc Feature(10)526..648(+)
Exon (1)1..141
Gene:CD40
Gene Synonym:
Exon (2)142..220
Gene:CD40
Gene Synonym:
Exon (3)221..346
Gene:CD40
Gene Synonym:
Exon (4)347..493
Gene:CD40
Gene Synonym:
Exon (5)494..587
Gene:CD40
Gene Synonym:
Exon (6)588..689
Gene:CD40
Gene Synonym:
Exon (7)690..776
Gene:CD40
Gene Synonym:
Exon (8)777..805
Gene:CD40
Gene Synonym:
Exon (9)806..1669
Gene:CD40
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001302753
Organism Homo sapiens (human)
Definition Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302753

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGCTG TGAGACCAAA GACCTGGTTG TGCAACAGGC AGGCACAAAC
AAGACTGATG TTGTCTGTGG TGAGTCCTGG ACAATGGGCC CTGGAGAAAG CCTAGGAAGG
TCCCCAGGAT CGGCTGAGAG CCCTGGTGGT GATCCCCATC ATCTTCGGGA TCCTGTTTGC
CATCCTCTTG GTGCTGGTCT TTATCAAAAA GGTGGCCAAG AAGCCAACCA ATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu12389
Clone ID Related Accession (Same CDS sequence) NM_152854
Accession Version NM_152854.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 612bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product tumor necrosis factor receptor superfamily member 5 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY063089.1, BM761221.1, BC012419.1, BC064518.1 and BX364284.2. On Nov 14, 2014 this sequence version replaced gi:91105277. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) contains a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ300189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGGTC CCCAGGATCG GCTGAGAGCC CTGGTGGTGA TCCCCATCAT
CTTCGGGATC CTGTTTGCCA TCCTCTTGGT GCTGGTCTTT ATCAAAAAGG TGGCCAAGAA
GCCAACCAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_690593.1
CDS91..702
Misc Feature(1)163..270(+)
Misc Feature(2)166..588(+)
Misc Feature(3)166..267(+)
Misc Feature(4)271..399(+)
Misc Feature(5)274..399(+)
Misc Feature(6)283..447(+)
Misc Feature(7)400..522(+)
Misc Feature(8)403..519(+)
Misc Feature(9)526..588(+)
Exon (1)1..141
Gene:CD40
Gene Synonym:
Exon (2)142..220
Gene:CD40
Gene Synonym:
Exon (3)221..346
Gene:CD40
Gene Synonym:
Exon (4)347..493
Gene:CD40
Gene Synonym:
Exon (5)494..587
Gene:CD40
Gene Synonym:
Exon (6)588..674
Gene:CD40
Gene Synonym:
Exon (7)675..703
Gene:CD40
Gene Synonym:
Exon (8)704..1567
Gene:CD40
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_152854
Organism Homo sapiens (human)
Definition Homo sapiens CD40 molecule, TNF receptor superfamily member 5 (CD40), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_152854

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGTTCGTC TGCCTCTGCA GTGCGTCCTC TGGGGCTGCT TGCTGACCGC TGTCCATCCA 
GAACCACCCA CTGCATGCAG AGAAAAACAG TACCTAATAA ACAGTCAGTG CTGTTCTTTG
TGCCAGCCAG GACAGAAACT GGTGAGTGAC TGCACAGAGT TCACTGAAAC GGAATGCCTT
CCTTGCGGTG AAAGCGAATT CCTAGACACC TGGAACAGAG AGACACACTG CCACCAGCAC
AAATACTGCG ACCCCAACCT AGGGCTTCGG GTCCAGCAGA AGGGCACCTC AGAAACAGAC
ACCATCTGCA CCTGTGAAGA AGGCTGGCAC TGTACGAGTG AGGCCTGTGA GAGCTGTGTC
CTGCACCGCT CATGCTCGCC CGGCTTTGGG GTCAAGCAGA TTGCTACAGG GGTTTCTGAT
ACCATCTGCG AGCCCTGCCC AGTCGGCTTC TTCTCCAATG TGTCATCTGC TTTCGAAAAA
TGTCACCCTT GGACAAGGTC CCCAGGATCG GCTGAGAGCC CTGGTGGTGA TCCCCATCAT
CTTCGGGATC CTGTTTGCCA TCCTCTTGGT GCTGGTCTTT ATCAAAAAGG TGGCCAAGAA
GCCAACCAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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