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MAML1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAML1
Entrez Gene ID 9794
Full Name mastermind-like transcriptional coactivator 1
Synonyms Mam-1, Mam1
General protein information
Preferred Names
mastermind-like protein 1
Names
mastermind-like protein 1
mastermind homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q35

Summary This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

605424


mRNA and Protein(s)

mRNA Protein Name
NM_014757 NP_055572 mastermind-like protein 1



Homo sapiens (human) MAML1 NP_055572.1
Pan troglodytes (chimpanzee) MAML1 XP_527150.3
Macaca mulatta (Rhesus monkey) MAML1 XP_001105097.2
Canis lupus familiaris (dog) MAML1 XP_005626406.1
Bos taurus (cattle) MAML1 XP_005208449.1
Mus musculus (house mouse) Maml1 NP_780543.2
Rattus norvegicus (Norway rat) Maml1 NP_001100467.1
Gallus gallus (chicken) MAML1 XP_414607.4
Danio rerio (zebrafish) LOC567335 XP_005157273.1
Xenopus (Silurana) tropicalis (western clawed frog) maml1 XP_002941844.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAML1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAML1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu06660 NM_014757 Homo sapiens mastermind-like transcriptional coactivator 1 (MAML1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $559.30
$799.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu06660
Clone ID Related Accession (Same CDS sequence) NM_014757
Accession Version NM_014757.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 08-AUG-2015
Organism Homo sapiens (human)
Product mastermind-like protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D83785.1, AF221759.1 and AA975821.1. On Oct 2, 2008 this sequence version replaced gi:41350321. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D83785.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGTGCTGC CCACCTGCCC CATGGCGGAG TTCGCGCTGC CGCGGCACAG CGCGGTCATG 
GAGCGCCTTC GCCGGCGCAT CGAGCTGTGC CGGCGCCACC ACAGCACCTG CGAGGCCCGC
TACGAGGCCG TGTCGCCCGA GCGCCTGGAG CTGGAGCGCC AACACACCTT CGCCCTGCAC
CAGCGCTGCA TCCAGGCCAA GGCCAAGCGC GCCGGGAAGC ACAGGCAGCC GCCCGCCGCC
ACGGCCCCGG CGCCCGCCGC CCCGGCCCCG CGCCTGGACG CCGCTGACGG CCCCGAGCAC
GGCCGCCCGG CCACGCATCT TCATGATACA GTTAAGAGGA ATCTTGACAG CGCCACTTCC
CCTCAGAATG GCGATCAACA GAATGGCTAC GGGGACCTCT TTCCTGGGCA TAAGAAGACT
CGCCGGGAGG CCCCTCTGGG AGTTGCCATC TCTTCCAATG GACTGCCTCC AGCCTCCCCC
CTCGGTCAGT CTGACAAGCC TTCTGGAGCC GACGCCCTGC AGTCCAGTGG GAAGCACTCT
CTGGGGCTAG ACTCTCTCAA CAAAAAGCGT CTGGCTGACT CCAGCCTTCA CTTGAATGGA
GGCAGTAACC CCAGTGAGTC ATTTCCTCTG AGCCTGAATA AAGAACTGAA GCAGGAGCCT
GTCGAAGACC TGCCTTGCAT GATCACTGGG ACTGTCGGCT CCATATCGCA AAGCAACCTC
ATGCCAGACC TCAACCTTAA CGAGCAGGAG TGGAAGGAGC TCATCGAGGA GCTGAACAGG
TCGGTGCCCG ATGAAGACAT GAAGGACCTG TTTAATGAGG ACTTCGAGGA GAAGAAGGAC
CCAGAGTCTT CTGGCTCTGC CACACAAACC CCCTTGGCAC AGGACATTAA TATTAAGACG
GAATTCTCTC CAGCAGCCTT TGAGCAAGAA CAGTTAGGCT CTCCACAAGT GAGGGCCGGG
TCTGCAGGGC AGACCTTTCT GGGGCCTTCC TCTGCCCCTG TGAGTACAGA TTCCCCCAGC
CTAGGGGGCT CCCAAACCTT ATTCCACACC TCTGGTCAGC CCCGGGCGGA CAATCCCAGT
CCAAACCTGA TGCCGGCATC AGCCCAGGCC CAGAACGCAC AAAGAGCCCT TGCAGGTGTG
GTATTGCCCA GTCAGGGCCC AGGAGGGGCC TCAGAGCTGT CCTCTGCCCA CCAGCTCCAG
CAGATCGCTG CCAAGCAGAA GCGCGAGCAG ATGCTCCAGA ACCCACAGCA GGCCACCCCG
GCACCAGCCC CGGGCCAGAT GTCCACATGG CAGCAGACGG GGCCCTCCCA CAGTTCCTTA
GATGTCCCTT ACCCCATGGA GAAGCCTGCC AGCCCTTCCA GCTACAAGCA AGACTTCACT
AACTCCAAAC TGCTCATGAT GCCTAGTGTG AATAAGAGTT CCCCTCGGCC CGGAGGCCCC
TACCTCCAGC CCAGCCATGT GAACCTGCTG AGTCACCAGC CACCGAGTAA CTTGAATCAG
AACTCCGCGA ATAACCAGGG GTCTGTGCTG GACTACGGCA ATACAAAACC CCTTTCTCAT
TACAAAGCGG ACTGTGGGCA AGGCAGCCCG GGGTCTGGCC AGAGCAAGCC AGCCCTGATG
GCTTATCTTC CCCAGCAGCT GTCCCATATA AGTCACGAGC AGAACTCCCT GTTTCTGATG
AAGCCAAAGC CAGGAAATAT GCCTTTCCGA TCACTGGTTC CACCTGGCCA GGAGCAGAAC
CCTTCCAGTG TCCCTGTGCA AGCCCAGGCT ACCAGTGTTG GGACCCAGCC GCCTGCCGTG
TCCGTGGCCA GCTCCCACAA CAGCTCCCCC TATCTCAGCA GCCAGCAACA GGCCGCTGTA
ATGAAGCAGC ATCAGTTGCT TTTGGACCAA CAGAAACAAA GGGAGCAGCA GCAAAAGCAT
TTACAGCAAC AGCAGTTCCT TCAGAGGCAA CAGCACCTTC TCGCGGAACA GGAGAAGCAA
CAGTTTCAGC GCCATCTGAC CCGCCCACCA CCCCAGTACC AAGACCCGAC ACAAGGCAGC
TTCCCACAGC AGGTTGGACA GTTCACAGGG TCCTCTGCTG CCGTGCCCGG CATGAACACC
TTGGGTCCAT CCAACTCCAG CTGTCCTCGA GTGTTCCCTC AGGCTGGGAA TCTGATGCCA
ATGGGCCCTG GACATGCTTC AGTTTCCTCT CTCCCCACAA ACTCAGGCCA ACAGGACCGG
GGTGTGGCTC AGTTCCCTGG CTCCCAAAAC ATGCCTCAGA GCAGCCTCTA TGGCATGGCT
TCTGGCATAA CCCAGATAGT TGCCCAGCCC CCGCCACAGG CCACCAATGG ACATGCCCAC
ATTCCACGGC AGACCAACGT GGGCCAGAAC ACCTCCGTCT CAGCTGCCTA TGGGCAGAAC
TCTCTGGGAA GCTCTGGCCT CTCCCAGCAG CACAATAAGG GGACCCTGAA CCCTGGTTTA
ACAAAGCCAC CGGTCCCAAG GGTGTCACCA GCCATGGGAG GCCAGAATTC CTCCTGGCAG
CATCAGGGAA TGCCGAACCT CAGTGGCCAG ACCCCAGGGA ACAGCAACGT GAGTCCCTTC
ACTGCAGCCT CCAGTTTCCA CATGCAGCAG CAGGCCCACC TGAAAATGTC TAGCCCGCAA
TTCTCCCAGG CAGTGCCCAA CAGGCCCATG GCTCCCATGA GCTCAGCAGC TGCCGTGGGG
TCCTTGCTAC CCCCAGTGAG TGCACAGCAG AGGACCAGCG CCCCTGCCCC AGCACCACCC
CCAACAGCCC CTCAGCAGGG CTTGCCTGGC CTGAGCCCAG CAGGGCCTGA GCTGGGGGCC
TTCAGCCAGA GCCCTGCCTC ACAGATGGGC GGTCGGGCGG GGCTGCACTG CACCCAGGCC
TACCCTGTGC GGACCGCGGG CCAGGAGCTG CCTTTTGCCT ATAGCGGGCA GCCAGGTGGC
AGTGGGCTCT CTAGTGTGGC TGGACACACC GATCTGATCG ACTCCCTGCT GAAGAACAGG
ACTTCAGAGG AGTGGATGAG TGATTTGGAC GACCTGTTAG GGTCTCAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055572.1
CDS264..3314
Misc Feature(1)183..185(+)
Misc Feature(2)264..632(+)
Misc Feature(3)303..482(+)
Misc Feature(4)621..623(+)
Misc Feature(5)1203..1205(+)
Misc Feature(6)1230..1895(+)
Misc Feature(7)1341..1343(+)
Misc Feature(8)2727..2729(+)
Misc Feature(9)3306..3308(+)
Exon (1)1..578
Gene:MAML1
Gene Synonym:
Exon (2)579..1994
Gene:MAML1
Gene Synonym:
Exon (3)1995..2234
Gene:MAML1
Gene Synonym:
Exon (4)2235..2331
Gene:MAML1
Gene Synonym:
Exon (5)2332..5723
Gene:MAML1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_014757
Organism Homo sapiens (human)
Definition Homo sapiens mastermind-like transcriptional coactivator 1 (MAML1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_014757

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGGTGCTGC CCACCTGCCC CATGGCGGAG TTCGCGCTGC CGCGGCACAG CGCGGTCATG 
GAGCGCCTTC GCCGGCGCAT CGAGCTGTGC CGGCGCCACC ACAGCACCTG CGAGGCCCGC
TACGAGGCCG TGTCGCCCGA GCGCCTGGAG CTGGAGCGCC AACACACCTT CGCCCTGCAC
CAGCGCTGCA TCCAGGCCAA GGCCAAGCGC GCCGGGAAGC ACAGGCAGCC GCCCGCCGCC
ACGGCCCCGG CGCCCGCCGC CCCGGCCCCG CGCCTGGACG CCGCTGACGG CCCCGAGCAC
GGCCGCCCGG CCACGCATCT TCATGATACA GTTAAGAGGA ATCTTGACAG CGCCACTTCC
CCTCAGAATG GCGATCAACA GAATGGCTAC GGGGACCTCT TTCCTGGGCA TAAGAAGACT
CGCCGGGAGG CCCCTCTGGG AGTTGCCATC TCTTCCAATG GACTGCCTCC AGCCTCCCCC
CTCGGTCAGT CTGACAAGCC TTCTGGAGCC GACGCCCTGC AGTCCAGTGG GAAGCACTCT
CTGGGGCTAG ACTCTCTCAA CAAAAAGCGT CTGGCTGACT CCAGCCTTCA CTTGAATGGA
GGCAGTAACC CCAGTGAGTC ATTTCCTCTG AGCCTGAATA AAGAACTGAA GCAGGAGCCT
GTCGAAGACC TGCCTTGCAT GATCACTGGG ACTGTCGGCT CCATATCGCA AAGCAACCTC
ATGCCAGACC TCAACCTTAA CGAGCAGGAG TGGAAGGAGC TCATCGAGGA GCTGAACAGG
TCGGTGCCCG ATGAAGACAT GAAGGACCTG TTTAATGAGG ACTTCGAGGA GAAGAAGGAC
CCAGAGTCTT CTGGCTCTGC CACACAAACC CCCTTGGCAC AGGACATTAA TATTAAGACG
GAATTCTCTC CAGCAGCCTT TGAGCAAGAA CAGTTAGGCT CTCCACAAGT GAGGGCCGGG
TCTGCAGGGC AGACCTTTCT GGGGCCTTCC TCTGCCCCTG TGAGTACAGA TTCCCCCAGC
CTAGGGGGCT CCCAAACCTT ATTCCACACC TCTGGTCAGC CCCGGGCGGA CAATCCCAGT
CCAAACCTGA TGCCGGCATC AGCCCAGGCC CAGAACGCAC AAAGAGCCCT TGCAGGTGTG
GTATTGCCCA GTCAGGGCCC AGGAGGGGCC TCAGAGCTGT CCTCTGCCCA CCAGCTCCAG
CAGATCGCTG CCAAGCAGAA GCGCGAGCAG ATGCTCCAGA ACCCACAGCA GGCCACCCCG
GCACCAGCCC CGGGCCAGAT GTCCACATGG CAGCAGACGG GGCCCTCCCA CAGTTCCTTA
GATGTCCCTT ACCCCATGGA GAAGCCTGCC AGCCCTTCCA GCTACAAGCA AGACTTCACT
AACTCCAAAC TGCTCATGAT GCCTAGTGTG AATAAGAGTT CCCCTCGGCC CGGAGGCCCC
TACCTCCAGC CCAGCCATGT GAACCTGCTG AGTCACCAGC CACCGAGTAA CTTGAATCAG
AACTCCGCGA ATAACCAGGG GTCTGTGCTG GACTACGGCA ATACAAAACC CCTTTCTCAT
TACAAAGCGG ACTGTGGGCA AGGCAGCCCG GGGTCTGGCC AGAGCAAGCC AGCCCTGATG
GCTTATCTTC CCCAGCAGCT GTCCCATATA AGTCACGAGC AGAACTCCCT GTTTCTGATG
AAGCCAAAGC CAGGAAATAT GCCTTTCCGA TCACTGGTTC CACCTGGCCA GGAGCAGAAC
CCTTCCAGTG TCCCTGTGCA AGCCCAGGCT ACCAGTGTTG GGACCCAGCC GCCTGCCGTG
TCCGTGGCCA GCTCCCACAA CAGCTCCCCC TATCTCAGCA GCCAGCAACA GGCCGCTGTA
ATGAAGCAGC ATCAGTTGCT TTTGGACCAA CAGAAACAAA GGGAGCAGCA GCAAAAGCAT
TTACAGCAAC AGCAGTTCCT TCAGAGGCAA CAGCACCTTC TCGCGGAACA GGAGAAGCAA
CAGTTTCAGC GCCATCTGAC CCGCCCACCA CCCCAGTACC AAGACCCGAC ACAAGGCAGC
TTCCCACAGC AGGTTGGACA GTTCACAGGG TCCTCTGCTG CCGTGCCCGG CATGAACACC
TTGGGTCCAT CCAACTCCAG CTGTCCTCGA GTGTTCCCTC AGGCTGGGAA TCTGATGCCA
ATGGGCCCTG GACATGCTTC AGTTTCCTCT CTCCCCACAA ACTCAGGCCA ACAGGACCGG
GGTGTGGCTC AGTTCCCTGG CTCCCAAAAC ATGCCTCAGA GCAGCCTCTA TGGCATGGCT
TCTGGCATAA CCCAGATAGT TGCCCAGCCC CCGCCACAGG CCACCAATGG ACATGCCCAC
ATTCCACGGC AGACCAACGT GGGCCAGAAC ACCTCCGTCT CAGCTGCCTA TGGGCAGAAC
TCTCTGGGAA GCTCTGGCCT CTCCCAGCAG CACAATAAGG GGACCCTGAA CCCTGGTTTA
ACAAAGCCAC CGGTCCCAAG GGTGTCACCA GCCATGGGAG GCCAGAATTC CTCCTGGCAG
CATCAGGGAA TGCCGAACCT CAGTGGCCAG ACCCCAGGGA ACAGCAACGT GAGTCCCTTC
ACTGCAGCCT CCAGTTTCCA CATGCAGCAG CAGGCCCACC TGAAAATGTC TAGCCCGCAA
TTCTCCCAGG CAGTGCCCAA CAGGCCCATG GCTCCCATGA GCTCAGCAGC TGCCGTGGGG
TCCTTGCTAC CCCCAGTGAG TGCACAGCAG AGGACCAGCG CCCCTGCCCC AGCACCACCC
CCAACAGCCC CTCAGCAGGG CTTGCCTGGC CTGAGCCCAG CAGGGCCTGA GCTGGGGGCC
TTCAGCCAGA GCCCTGCCTC ACAGATGGGC GGTCGGGCGG GGCTGCACTG CACCCAGGCC
TACCCTGTGC GGACCGCGGG CCAGGAGCTG CCTTTTGCCT ATAGCGGGCA GCCAGGTGGC
AGTGGGCTCT CTAGTGTGGC TGGACACACC GATCTGATCG ACTCCCTGCT GAAGAACAGG
ACTTCAGAGG AGTGGATGAG TGATTTGGAC GACCTGTTAG GGTCTCAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Insight into genetic determinants of resting heart rate
Gene 545 (1), 170-174 (2014)
Mezzavilla M, Iorio A, Bobbo M, D'Eustacchio A, Merlo M, Gasparini P, Ulivi S and Sinagra G.


book

The human papillomavirus type 8 E6 protein interferes with NOTCH activation during keratinocyte differentiation
J. Virol. 87 (8), 4762-4767 (2013)
Meyers JM, Spangle JM and Munger K.


book

Snail inhibits Notch1 intracellular domain mediated transcriptional activation via competing with MAML1
Biochem. Biophys. Res. Commun. 433 (1), 6-10 (2013)
Kim HS, Jeong H, Lim SO and Jung G.


book

Circulating tumor cells (CTCs) detected by triple-marker EpCAM, CK19, and hMAM RT-PCR and their relation to clinical outcome in metastatic breast cancer patients
Cell Biochem. Biophys. 65 (2), 263-273 (2013)
Zhao S, Yang H, Zhang M, Zhang D, Liu Y, Liu Y, Song Y, Zhang X, Li H, Ma W and Zhang Q.


book

MAML1 acts cooperatively with EGR1 to activate EGR1-regulated promoters: implications for nephrogenesis and the development of renal cancer
PLoS ONE 7 (9), E46001 (2012)
Hansson ML, Behmer S, Ceder R, Mohammadi S, Preta G, Grafstrom RC, Fadeel B and Wallberg AE.


book

Mastermind mediates chromatin-specific transcription and turnover of the Notch enhancer complex
Genes Dev. 16 (11), 1397-1411 (2002)
Fryer CJ, Lamar E, Turbachova I, Kintner C and Jones KA.


book

SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation
Mol. Cell. Biol. 21 (21), 7403-7415 (2001)
Wu G, Lyapina S, Das I, Li J, Gurney M, Pauley A, Chui I, Deshaies RJ and Kitajewski J.


book

A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters
Mol. Cell. Biol. 21 (13), 4337-4346 (2001)
Kitagawa M, Oyama T, Kawashima T, Yedvobnick B, Kumar A, Matsuno K and Harigaya K.


book

MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors
Nat. Genet. 26 (4), 484-489 (2000)
Wu L, Aster JC, Blacklow SC, Lake R, Artavanis-Tsakonas S and Griffin JD.


book

CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex
Proc. Natl. Acad. Sci. U.S.A. 96 (1), 23-28 (1999)
Hsieh JJ, Zhou S, Chen L, Young DB and Hayward SD.


 
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