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RBM8A cDNA ORF clone, Homo sapiens (human)

Gene Symbol RBM8A
Entrez Gene ID 9939
Full Name RNA binding motif protein 8A
Synonyms BOV-1A, BOV-1B, BOV-1C, C1DELq21.1, DEL1q21.1, MDS014, RBM8, RBM8B, TAR, Y14, ZNRP, ZRNP1
General protein information
Preferred Names
RNA-binding protein 8A
Names
RNA-binding protein 8A
BOV-1
binder of OVCA1-1
ribonucleoprotein RBM8
RNA-binding protein Y14
ribonucleoprotein RBM8A
RNA binding motif protein 8B
RNA-binding motif protein 8A
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q21.1

Summary This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. There are two alternate translation start codons with this gene, which result in two forms of the protein. An allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in this gene cause thrombocytopenia-absent radius (TAR) syndrome. [provided by RefSeq, Jul 2013]. lac of sum
Disorder MIM:

605313


mRNA and Protein(s)

mRNA Protein Name
NM_005105 NP_005096 RNA-binding protein 8A



Homo sapiens (human) RBM8A NP_005096.1
Pan troglodytes (chimpanzee) RBM8A XP_001162472.1
Canis lupus familiaris (dog) RBM8A XP_533035.1
Bos taurus (cattle) RBM8A NP_001030401.1
Mus musculus (house mouse) Rbm8a NP_001095877.1
Rattus norvegicus (Norway rat) Rbm8a NP_001258067.1
Danio rerio (zebrafish) rbm8a NP_001013363.1
Drosophila melanogaster (fruit fly) tsu NP_610454.2
Caenorhabditis elegans rnp-4 NP_497891.1
Arabidopsis thaliana (thale cress) Y14 NP_564591.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RBM8A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RBM8A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu20462
NM_005105 Homo sapiens RNA binding motif protein 8A (RBM8A), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20462
Clone ID Related Accession (Same CDS sequence) NM_005105
Accession Version NM_005105.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 525bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product RNA-binding protein 8A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB286556.1, AF127761.1, AC243547.1 and AI424454.1. On Jul 19, 2013 this sequence version replaced gi:324120942. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071577.1, AK075009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
ATGGCGGACG TGCTAGATCT TCACGAGGCT GGGGGCGAAG ATTTCGCCAT GGATGAGGAT 
GGGGACGAGA GCATTCACAA ACTGAAAGAA AAAGCGAAGA AACGGAAGGG TCGCGGCTTT
GGCTCCGAAG AGGGGTCCCG AGCGCGGATG CGTGAGGATT ATGACAGCGT GGAGCAGGAT
GGCGATGAAC CCGGACCACA ACGCTCTGTT GAAGGCTGGA TTCTCTTTGT AACTGGAGTC
CATGAGGAAG CCACCGAAGA AGACATACAC GACAAATTCG CAGAATATGG GGAAATTAAA
AACATTCATC TCAACCTCGA CAGGCGAACA GGATATCTGA AGGGGTATAC TCTAGTTGAA
TATGAAACAT ACAAGGAAGC CCAGGCTGCT ATGGAGGGAC TCAATGGCCA GGATTTGATG
GGACAGCCCA TCAGCGTTGA CTGGTGTTTT GTTCGGGGTC CACCAAAAGG CAAGAGGAGA
GGTGGCCGAA GACGCAGCAG AAGTCCAGAC CGGAGACGTC GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005096.1
CDS111..635
Misc Feature(1)69..71(+)
Misc Feature(2)114..116(+)
Misc Feature(3)180..182(+)
Misc Feature(4)234..236(+)
Misc Feature(5)234..236(+)
Misc Feature(6)246..248(+)
Misc Feature(7)276..278(+)
Misc Feature(8)309..563(+)
Misc Feature(9)309..563(+)
Misc Feature(10)333..548(+)
Misc Feature(11)606..608(+)
Misc Feature(12)612..614(+)
Misc Feature(13)159..161(+)
Exon (1)1..177
Gene:RBM8A
Gene Synonym:
Exon (2)178..237
Gene:RBM8A
Gene Synonym:
Exon (3)238..315
Gene:RBM8A
Gene Synonym:
Exon (4)316..452
Gene:RBM8A
Gene Synonym:
Exon (5)453..589
Gene:RBM8A
Gene Synonym:
Exon (6)590..4961
Gene:RBM8A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005105
Organism Homo sapiens (human)
Definition Homo sapiens RNA binding motif protein 8A (RBM8A), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005105

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCGGACG TGCTAGATCT TCACGAGGCT GGGGGCGAAG ATTTCGCCAT GGATGAGGAT 
GGGGACGAGA GCATTCACAA ACTGAAAGAA AAAGCGAAGA AACGGAAGGG TCGCGGCTTT
GGCTCCGAAG AGGGGTCCCG AGCGCGGATG CGTGAGGATT ATGACAGCGT GGAGCAGGAT
GGCGATGAAC CCGGACCACA ACGCTCTGTT GAAGGCTGGA TTCTCTTTGT AACTGGAGTC
CATGAGGAAG CCACCGAAGA AGACATACAC GACAAATTCG CAGAATATGG GGAAATTAAA
AACATTCATC TCAACCTCGA CAGGCGAACA GGATATCTGA AGGGGTATAC TCTAGTTGAA
TATGAAACAT ACAAGGAAGC CCAGGCTGCT ATGGAGGGAC TCAATGGCCA GGATTTGATG
GGACAGCCCA TCAGCGTTGA CTGGTGTTTT GTTCGGGGTC CACCAAAAGG CAAGAGGAGA
GGTGGCCGAA GACGCAGCAG AAGTCCAGAC CGGAGACGTC GCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Slow co-evolution of the MAGO and Y14 protein families is required for the maintenance of their obligate heterodimerization mode
PLoS ONE 9 (1), E84842 (2014)
Gong P, Zhao M and He C.


book

Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human cells
Exp. Biol. Med. (Maywood) 238 (8), 889-897 (2013)
Ishigaki Y, Nakamura Y, Tatsuno T, Hashimoto M, Shimasaki T, Iwabuchi K and Tomosugi N.


book

Y14 positively regulates TNF-alpha-induced NF-kappaB transcriptional activity via interacting RIP1 and TRADD beyond an exon junction complex protein
J. Immunol. 191 (3), 1436-1444 (2013)
Togi S, Shiga K, Muromoto R, Kato M, Souma Y, Sekine Y, Kon S, Oritani K and Matsuda T.


book

The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation
Mol. Biol. Cell 24 (1), 1-13 (2013)
Chuang TW, Chang WL, Lee KM and Tarn WY.


book

Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome
Nat. Genet. 44 (4), 435-439 (2012)
Albers CA, Paul DS, Schulze H, Freson K, Stephens JC, Smethurst PA, Jolley JD, Cvejic A, Kostadima M, Bertone P, Breuning MH, Debili N, Deloukas P, Favier R, Fiedler J, Hobbs CM, Huang N, Hurles ME, Kiddle G, Krapels I, Nurden P, Ruivenkamp CA, Sambrook JG, Smith K, Stemple DL, Strauss G, Thys C, van Geet C, Newbury-Ecob R, Ouwehand WH and Ghevaert C.


book

Identification and structural analysis of human RBM8A and RBM8B: two highly conserved RNA-binding motif proteins that interact with OVCA1, a candidate tumor suppressor
Genomics 69 (1), 54-62 (2000)
Salicioni AM, Xi M, Vanderveer LA, Balsara B, Testa JR, Dunbrack RL Jr and Godwin AK.


book

Cloning and gene expression of a novel human ribonucleoprotein
Biochim. Biophys. Acta 1492 (2-3), 465-469 (2000)
Conklin DC, Rixon MW, Kuestner RE, Maurer MF, Whitmore TE and Millar RP.


book

MAGOH interacts with a novel RNA-binding protein
Genomics 63 (1), 145-148 (2000)
Zhao XF, Nowak NJ, Shows TB and Aplan PD.


book

The nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction
J. Biol. Chem. 274 (7), 4166-4173 (1999)
Wilson KF, Fortes P, Singh US, Ohno M, Mattaj IW and Cerione RA.


book

Thrombocytopenia Absent Radius Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Toriello,H.V.


 
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