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MED12 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MED12
Entrez Gene ID 9968
Full Name mediator complex subunit 12
Synonyms ARC240, CAGH45, FGS1, HOPA, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230
General protein information
Preferred Names
mediator of RNA polymerase II transcription subunit 12
Names
mediator of RNA polymerase II transcription subunit 12
CAG repeat protein 45
human opposite paired
OPA-containing protein
putative mediator subunit 12
activator-recruited cofactor 240 kDa component
trinucleotide repeat-containing gene 11 protein
thyroid hormone receptor-associated protein, 230 kDa subunit
mediator of RNA polymerase II transcription, subunit 12 homolog
thyroid hormone receptor-associated protein complex 230 kDa component
trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq13

Summary The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]. lac of sum
Disorder MIM:

300188

Disorder Html: Opitz-Kaveggia syndrome, 305450 (3); Lujan-Fryns syndrome, 309520 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005262317 XP_005262374 mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_005262319 XP_005262376 mediator of RNA polymerase II transcription subunit 12 isoform X2
NM_005120 NP_005111 mediator of RNA polymerase II transcription subunit 12



Homo sapiens (human) MED12 NP_005111.2
Pan troglodytes (chimpanzee) MED12 NP_001009019.1
Macaca mulatta (Rhesus monkey) MED12 XP_001088424.2
Canis lupus familiaris (dog) MED12 XP_003640267.1
Bos taurus (cattle) MED12 NP_001192807.1
Mus musculus (house mouse) Med12 NP_067496.2
Rattus norvegicus (Norway rat) Med12 NP_001180221.1
Gallus gallus (chicken) MED12 XP_004940691.1
Danio rerio (zebrafish) med12 NP_001034550.1
Xenopus (Silurana) tropicalis (western clawed frog) med12 XP_002934949.2


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu54272 XM_005262317 PREDICTED: Homo sapiens mediator complex subunit 12 (MED12), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1091.30
$1559.00
OHu54273 XM_005262319 PREDICTED: Homo sapiens mediator complex subunit 12 (MED12), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1091.30
$1559.00
OHu19668 NM_005120 Homo sapiens mediator complex subunit 12 (MED12), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1091.30
$1559.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu54272
Clone ID Related Accession (Same CDS sequence) XM_005262317
Accession Version XM_005262317.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011651.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTACCA GAGCACCCAC
CCTTCTACCA ATCCTACTCT TGTAGATCCT ACCCGCCACC TGCAACAGCG GCCCAGTGGC
TATGTGCACC AGCAGGCCCC CACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA
CACCAGACAC TGCAGCAGAC ACCCATGATA AGTACCATGA CTCCAATGAG TGCCCAGGGC
GTCCAGGCAG GCGTCCGTTC AACAGCCATC CTACCTGAGC AGCAGCAGCA GCAGCAACAG
CAGCAACAGC AACAGCAGCA GCAGCAGCAA CAGCAACAGC AGCAGCAGCA GCAGCAGTAC
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGCAGC AGCAGCAACA GCAACAGCAG
CAGCAGCAGC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGCAACAACA GCAACACCAG
CAGCAACAGC AGCAACAGGC GGCTCCTCCC CAACCCCAGC CCCAGTCCCA GCCCCAGTTC
CAGCGCCAGG GGCTTCAGCA GACCCAGCAG CAGCAACAGA CAGCAGCTTT GGTCCGGCAA
CTTCAACAAC AGCTCTCTAA TACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262374.1
CDS200..6742
Misc Feature(1)500..682(+)
Misc Feature(2)1052..2470(+)
Misc Feature(3)5648..6268(+)
Translation

Target ORF information:

RefSeq Version XM_005262317
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mediator complex subunit 12 (MED12), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262317

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA
GGCCCTGCAG GTACCATGGT GCCCCCCAGC TACTCCAGCC AGCCTTACCA GAGCACCCAC
CCTTCTACCA ATCCTACTCT TGTAGATCCT ACCCGCCACC TGCAACAGCG GCCCAGTGGC
TATGTGCACC AGCAGGCCCC CACCTATGGA CATGGACTGA CCTCCACTCA AAGGTTTTCA
CACCAGACAC TGCAGCAGAC ACCCATGATA AGTACCATGA CTCCAATGAG TGCCCAGGGC
GTCCAGGCAG GCGTCCGTTC AACAGCCATC CTACCTGAGC AGCAGCAGCA GCAGCAACAG
CAGCAACAGC AACAGCAGCA GCAGCAGCAA CAGCAACAGC AGCAGCAGCA GCAGCAGTAC
CACATCCGGC AGCAGCAGCA GCAGCAGATC CTGCGGCAGC AGCAGCAACA GCAACAGCAG
CAGCAGCAGC AGCAGCAACA GCAACAGCAG CAGCAGCAAC AGCAACAACA GCAACACCAG
CAGCAACAGC AGCAACAGGC GGCTCCTCCC CAACCCCAGC CCCAGTCCCA GCCCCAGTTC
CAGCGCCAGG GGCTTCAGCA GACCCAGCAG CAGCAACAGA CAGCAGCTTT GGTCCGGCAA
CTTCAACAAC AGCTCTCTAA TACCCAGCCA CAGCCCAGTA CCAACATATT TGGACGCTAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54273
Clone ID Related Accession (Same CDS sequence) XM_005262319
Accession Version XM_005262319.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6468bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011651.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA
GGCCCTGCAG ATCCTACCCG CCACCTGCAA CAGCGGCCCA GTGGCTATGT GCACCAGCAG
GCCCCCACCT ATGGACATGG ACTGACCTCC ACTCAAAGGT TTTCACACCA GACACTGCAG
CAGACACCCA TGATAAGTAC CATGACTCCA ATGAGTGCCC AGGGCGTCCA GGCAGGCGTC
CGTTCAACAG CCATCCTACC TGAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG
CAGCAGCAGC AGCAACAGCA ACAGCAGCAG CAGCAGCAGC AGTACCACAT CCGGCAGCAG
CAGCAGCAGC AGATCCTGCG GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG
CAACAGCAAC AGCAGCAGCA GCAACAGCAA CAACAGCAAC ACCAGCAGCA ACAGCAGCAA
CAGGCGGCTC CTCCCCAACC CCAGCCCCAG TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT
CAGCAGACCC AGCAGCAGCA ACAGACAGCA GCTTTGGTCC GGCAACTTCA ACAACAGCTC
TCTAATACCC AGCCACAGCC CAGTACCAAC ATATTTGGAC GCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005262376.1
CDS200..6667
Misc Feature(1)500..682(+)
Misc Feature(2)1052..2470(+)
Misc Feature(3)5648..6193(+)
Translation

Target ORF information:

RefSeq Version XM_005262319
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mediator complex subunit 12 (MED12), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005262319

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGGC AAAGATACAG
AGTCAGGGCA TGTTGGGACA GTCATCTGTC CATCAGATGA CTCCCAGCTC TTCCTACGGT
TTGCAGACTT CCCAGGGCTA TACTCCTTAT GTTTCTCATG TGGGATTGCA GCAACACACA
GGCCCTGCAG ATCCTACCCG CCACCTGCAA CAGCGGCCCA GTGGCTATGT GCACCAGCAG
GCCCCCACCT ATGGACATGG ACTGACCTCC ACTCAAAGGT TTTCACACCA GACACTGCAG
CAGACACCCA TGATAAGTAC CATGACTCCA ATGAGTGCCC AGGGCGTCCA GGCAGGCGTC
CGTTCAACAG CCATCCTACC TGAGCAGCAG CAGCAGCAGC AACAGCAGCA ACAGCAACAG
CAGCAGCAGC AGCAACAGCA ACAGCAGCAG CAGCAGCAGC AGTACCACAT CCGGCAGCAG
CAGCAGCAGC AGATCCTGCG GCAGCAGCAG CAACAGCAAC AGCAGCAGCA GCAGCAGCAG
CAACAGCAAC AGCAGCAGCA GCAACAGCAA CAACAGCAAC ACCAGCAGCA ACAGCAGCAA
CAGGCGGCTC CTCCCCAACC CCAGCCCCAG TCCCAGCCCC AGTTCCAGCG CCAGGGGCTT
CAGCAGACCC AGCAGCAGCA ACAGACAGCA GCTTTGGTCC GGCAACTTCA ACAACAGCTC
TCTAATACCC AGCCACAGCC CAGTACCAAC ATATTTGGAC GCTACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19668
Clone ID Related Accession (Same CDS sequence) NM_005120
Accession Version NM_005120.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6534bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mediator of RNA polymerase II transcription subunit 12
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB069668.1, AF117755.1, AF071309.1 and BM662470.1. This sequence is a reference standard in the RefSeqGene project. On Jul 14, 2006 this sequence version replaced gi:4827041. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA
GGCGTCCGTT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005111.2
CDS200..6733
Misc Feature(1)500..682(+)
Misc Feature(2)695..697(+)
Misc Feature(3)1052..2470(+)
Misc Feature(4)2102..2104(+)
Misc Feature(5)2102..2104(+)
Misc Feature(6)3971..3973(+)
Misc Feature(7)4004..4006(+)
Misc Feature(8)5045..6352(+)
Misc Feature(9)5648..6259(+)
Exon (1)1..298
Gene:MED12
Gene Synonym:
Exon (2)299..403
Gene:MED12
Gene Synonym:
Exon (3)404..595
Gene:MED12
Gene Synonym:
Exon (4)596..752
Gene:MED12
Gene Synonym:
Exon (5)753..934
Gene:MED12
Gene Synonym:
Exon (6)935..1045
Gene:MED12
Gene Synonym:
Exon (7)1046..1300
Gene:MED12
Gene Synonym:
Exon (8)1301..1447
Gene:MED12
Gene Synonym:
Exon (9)1448..1547
Gene:MED12
Gene Synonym:
Exon (10)1548..1684
Gene:MED12
Gene Synonym:
Exon (11)1685..1816
Gene:MED12
Gene Synonym:
Exon (12)1817..1943
Gene:MED12
Gene Synonym:
Exon (13)1944..2173
Gene:MED12
Gene Synonym:
Exon (14)2174..2254
Gene:MED12
Gene Synonym:
Exon (15)2255..2425
Gene:MED12
Gene Synonym:
Exon (16)2426..2570
Gene:MED12
Gene Synonym:
Exon (17)2571..2621
Gene:MED12
Gene Synonym:
Exon (18)2622..2740
Gene:MED12
Gene Synonym:
Exon (19)2741..2884
Gene:MED12
Gene Synonym:
Exon (20)2885..3048
Gene:MED12
Gene Synonym:
Exon (21)3049..3180
Gene:MED12
Gene Synonym:
Exon (22)3181..3408
Gene:MED12
Gene Synonym:
Exon (23)3409..3553
Gene:MED12
Gene Synonym:
Exon (24)3554..3674
Gene:MED12
Gene Synonym:
Exon (25)3675..3776
Gene:MED12
Gene Synonym:
Exon (26)3777..3890
Gene:MED12
Gene Synonym:
Exon (27)3891..4066
Gene:MED12
Gene Synonym:
Exon (28)4067..4246
Gene:MED12
Gene Synonym:
Exon (29)4247..4318
Gene:MED12
Gene Synonym:
Exon (30)4319..4452
Gene:MED12
Gene Synonym:
Exon (31)4453..4614
Gene:MED12
Gene Synonym:
Exon (32)4615..4726
Gene:MED12
Gene Synonym:
Exon (33)4727..4816
Gene:MED12
Gene Synonym:
Exon (34)4817..4926
Gene:MED12
Gene Synonym:
Exon (35)4927..5062
Gene:MED12
Gene Synonym:
Exon (36)5063..5224
Gene:MED12
Gene Synonym:
Exon (37)5225..5599
Gene:MED12
Gene Synonym:
Exon (38)5600..5750
Gene:MED12
Gene Synonym:
Exon (39)5751..5947
Gene:MED12
Gene Synonym:
Exon (40)5948..6025
Gene:MED12
Gene Synonym:
Exon (41)6026..6243
Gene:MED12
Gene Synonym:
Exon (42)6244..6466
Gene:MED12
Gene Synonym:
Exon (43)6467..6607
Gene:MED12
Gene Synonym:
Exon (44)6608..6689
Gene:MED12
Gene Synonym:
Exon (45)6690..6969
Gene:MED12
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005120
Organism Homo sapiens (human)
Definition Homo sapiens mediator complex subunit 12 (MED12), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005120

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
ATGGCGGCCT TCGGGATCTT GAGCTACGAA CACCGGCCCC TGAAGCGGCC GCGGCTGGGG 
CCTCCCGATG TTTACCCTCA GGACCCCAAA CAGAAGGAGG ATGAACTGAC GGCCTTGAAT
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ATGAGCATGG CAGTGCCAAG
AACGTCAGCT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATTAT TGCAGAGAAA
TTACGTTGTA ATACCCTTCC TGACACTGGT CGCAGGAAGC CCCAAGTGAA CCAGAAGGAT
AACTTCTGGC TGGTGACTGC ACGATCCCAG AGTGCCATTA ACACTTGGTT CACTGACTTG
GCTGGCACCA AGCCACTCAC GCAACTAGCC AAAAAGGTCC CCATTTTCAG TAAGAAGGAA
GAGGTGTTTG GGTACTTAGC CAAATACACA GTGCCTGTGA TGCGGGCTGC CTGGCTCATT
AAGATGACCT GTGCCTACTA TGCAGCAATC TCTGAGACCA AGGTTAAGAA GAGACATGTT
GACCCTTTCA TGGAATGGAC TCAGATCATC ACCAAGTACT TATGGGAGCA GTTACAGAAG
ATGGCTGAAT ACTACCGGCC AGGGCCTGCA GGAAGTGGGG GCTGTGGTTC CACGATAGGG
CCCTTGCCCC ATGATGTAGA GGTGGCAATC CGGCAGTGGG ATTACACCGA GAAGCTGGCC
ATGTTCATGT TTCAGGATGG AATGCTGGAC AGACATGAGT TCCTGACCTG GGTGCTTGAG
TGTTTTGAGA AGATCCGCCC TGGAGAGGAT GAATTGCTTA AACTGCTGCT GCCTCTGCTT
CTCCGATACT CTGGGGAATT TGTTCAGTCT GCATACCTGT CCCGCCGGCT TGCCTACTTC
TGTACACGGA GACTGGCCCT GCAGCTGGAT GGTGTGAGCA GTCACTCATC TCATGTTATA
TCTGCTCAGT CAACAAGCAC GCTACCCACC ACCCCTGCTC CTCAGCCCCC AACTAGCAGC
ACACCCTCGA CTCCCTTTAG TGACCTGCTT ATGTGCCCTC AGCACCGGCC CCTGGTTTTT
GGCCTCAGCT GTATCCTACA GACCATCCTC CTGTGCTGTC CTAGTGCCTT GGTTTGGCAC
TACTCACTGA CTGATAGCAG AATTAAGACC GGCTCACCAC TTGACCACTT GCCTATTGCC
CCGTCCAACC TGCCCATGCC AGAGGGTAAC AGTGCCTTCA CTCAGCAGGT CCGTGCAAAG
TTGCGGGAGA TCGAGCAGCA GATCAAGGAG CGGGGACAGG CAGTTGAAGT TCGCTGGTCT
TTCGATAAAT GCCAGGAAGC TACTGCAGGC TTCACCATTG GACGGGTACT TCATACTTTG
GAAGTGCTGG ACAGCCATAG TTTTGAACGC TCTGACTTCA GCAACTCTCT TGACTCCCTT
TGTAACCGAA TCTTTGGATT GGGACCTAGC AAGGATGGGC ATGAGATCTC CTCAGATGAT
GATGCTGTGG TGTCATTGCT ATGTGAATGG GCTGTCAGCT GCAAGCGTTC TGGTCGGCAT
CGTGCTATGG TGGTAGCCAA GCTCCTGGAG AAGAGACAGG CGGAGATTGA GGCTGAGCGT
TGTGGAGAAT CAGAAGCCGC AGATGAGAAG GGTTCCATCG CCTCTGGCTC CCTTTCTGCT
CCCAGTGCTC CCATTTTCCA GGATGTCCTC CTGCAGTTTC TGGATACACA GGCTCCCATG
CTGACGGACC CTCGAAGTGA GAGTGAGCGG GTGGAATTCT TTAACTTAGT ACTGCTGTTC
TGTGAACTGA TTCGACATGA TGTTTTCTCC CACAACATGT ATACTTGCAC TCTCATCTCC
CGAGGGGACC TTGCCTTTGG AGCCCCTGGT CCCCGGCCTC CCTCTCCCTT TGATGATCCT
GCCGATGACC CAGAGCACAA GGAGGCTGAA GGCAGCAGCA GCAGCAAGCT GGAAGATCCA
GGGCTCTCAG AATCTATGGA CATTGACCCT AGTTCCAGTG TTCTCTTTGA GGACATGGAG
AAGCCTGATT TCTCATTGTT CTCCCCTACT ATGCCCTGTG AGGGGAAGGG CAGTCCATCC
CCTGAGAAGC CAGATGTCGA GAAGGAGGTG AAGCCCCCAC CCAAGGAGAA GATTGAAGGG
ACCCTTGGGG TTCTTTACGA CCAGCCACGA CACGTGCAGT ACGCCACCCA TTTTCCCATC
CCCCAGGAGG AGTCATGCAG CCATGAGTGC AACCAGCGGT TGGTCGTACT GTTTGGGGTG
GGAAAGCAGC GAGATGATGC CCGCCATGCC ATCAAGAAAA TCACCAAGGA TATCTTGAAG
GTTCTGAACC GCAAAGGGAC AGCAGAAACT GACCAGCTTG CTCCTATTGT GCCTCTGAAT
CCTGGAGACC TGACATTCTT AGGTGGGGAG GATGGGCAGA AGCGGCGACG CAACCGGCCT
GAAGCCTTCC CCACTGCTGA AGATATCTTT GCTAAGTTCC AGCACCTTTC ACATTATGAC
CAACACCAGG TCACGGCTCA GGTCTCCCGG AATGTTCTGG AGCAGATCAC GAGCTTTGCC
CTTGGCATGT CATACCACTT GCCTCTGGTG CAGCATGTGC AGTTCATCTT CGACCTCATG
GAATATTCAC TCAGCATCAG TGGCCTCATC GACTTTGCCA TTCAGCTGCT GAATGAACTG
AGTGTAGTTG AGGCTGAGCT GCTTCTCAAA TCCTCGGATC TGGTGGGCAG CTACACTACT
AGCCTGTGCC TGTGCATCGT GGCTGTCCTG CGGCACTATC ATGCCTGCCT CATCCTCAAC
CAGGACCAGA TGGCACAGGT CTTTGAGGGG CTGTGTGGCG TCGTGAAGCA TGGGATGAAC
CGGTCCGATG GCTCCTCTGC AGAGCGCTGT ATCCTTGCTT ATCTCTATGA TCTGTACACC
TCCTGTAGCC ATTTAAAGAA CAAATTTGGG GAGCTCTTCA GCGACTTTTG CTCAAAGGTG
AAGAACACCA TCTACTGCAA CGTGGAGCCA TCGGAATCAA ATATGCGCTG GGCACCTGAG
TTCATGATCG ACACTCTAGA GAACCCTGCA GCTCACACCT TCACCTACAC GGGGCTAGGC
AAGAGTCTTA GTGAGAACCC TGCTAACCGC TACAGCTTTG TCTGCAATGC CCTTATGCAC
GTCTGTGTGG GGCACCATGA TCCCGATAGG GTGAATGACA TCGCAATCCT GTGTGCAGAG
CTGACCGGCT ATTGCAAGTC ACTGAGTGCA GAATGGCTAG GAGTGCTTAA GGCCTTGTGC
TGCTCCTCTA ACAATGGCAC TTGTGGTTTC AACGATCTCC TCTGCAATGT TGATGTCAGT
GACCTATCTT TTCATGACTC GCTGGCTACT TTTGTTGCCA TCCTCATCGC TCGGCAGTGT
TTGCTCCTGG AAGATCTGAT TCGCTGTGCT GCCATCCCTT CACTCCTTAA TGCTGCTTGT
AGTGAACAGG ACTCTGAGCC AGGGGCCCGG CTTACCTGCC GCATCCTCCT TCACCTTTTC
AAGACACCGC AGCTCAATCC TTGCCAGTCT GATGGAAACA AGCCTACAGT AGGAATCCGC
TCCTCCTGCG ACCGCCACCT GCTGGCTGCC TCCCAGAACC GCATCGTGGA TGGAGCCGTG
TTTGCTGTTC TCAAGGCTGT GTTTGTACTT GGGGATGCGG AACTGAAAGG TTCAGGCTTC
ACTGTGACAG GAGGAACAGA AGAACTTCCA GAGGAGGAGG GAGGAGGTGG CAGTGGTGGT
CGGAGGCAGG GTGGCCGCAA CATCTCTGTG GAGACAGCCA GTCTGGATGT CTATGCCAAG
TACGTGCTGC GCAGCATCTG CCAACAGGAA TGGGTAGGAG AACGTTGCCT TAAGTCTCTG
TGTGAGGACA GCAATGACCT GCAAGACCCA GTGTTGAGTA GTGCCCAGGC GCAGCGCCTC
ATGCAGCTCA TTTGCTATCC ACATCGACTG CTGGACAATG AGGATGGGGA AAACCCCCAG
CGGCAGCGCA TAAAGCGCAT TCTCCAGAAC TTGGACCAGT GGACCATGCG CCAGTCTTCC
TTGGAGCTGC AGCTCATGAT CAAGCAGACC CCTAACAATG AGATGAACTC CCTCTTGGAG
AACATCGCCA AGGCCACAAT CGAGGTTTTC CAACAGTCAG CAGAGACAGG GTCATCTTCT
GGAAGTACTG CAAGCAACAT GCCCAGCAGC AGCAAGACCA AGCCTGTGCT CAGCTCTCTA
GAGCGCTCTG GTGTATGGCT GGTGGCCCCC CTCATTGCTA AACTGCCCAC CTCAGTCCAG
GGACATGTGT TAAAGGCTGC TGGGGAAGAA TTGGAGAAGG GTCAGCACCT GGGTTCCTCT
TCACGCAAAG AACGTGATCG ACAAAAGCAG AAGAGCATGT CCCTATTGAG CCAGCAGCCC
TTCTTATCGC TGGTGCTAAC ATGTCTGAAA GGGCAGGATG AACAACGCGA GGGACTCCTT
ACCTCCCTCT ACAGCCAGGT GCACCAGATT GTGAATAATT GGCGAGATGA CCAGTACTTA
GATGATTGCA AACCAAAGCA GCTTATGCAT GAGGCACTCA AACTGCGGCT CAACCTGGTG
GGGGGCATGT TTGACACGGT GCAGCGCAGC ACCCAGCAGA CCACGGAGTG GGCCATGCTC
CTCCTGGAGA TCATCATCAG CGGCACTGTC GACATGCAGT CCAACAATGA GCTCTTCACT
ACTGTGTTGG ACATGCTGAG CGTGCTCATC AATGGGACAT TGGCTGCAGA CATGTCTAGC
ATCTCGCAAG GTAGCATGGA GGAAAACAAG CGTGCATACA TGAACCTGGC GAAGAAGTTG
CAGAAGGAGT TGGGGGAGCG CCAGTCAGAC AGTCTGGAAA AGGTTCGCCA GCTGCTGCCA
CTGCCCAAGC AGACCCGAGA TGTCATCACG TGTGAGCCAC AGGGCTCCCT TATCGATACC
AAGGGCAACA AGATTGCTGG CTTCGATTCC ATCTTCAAGA AGGAGGGTCT ACAGGTTTCC
ACCAAACAGA AGATCTCGCC CTGGGATCTT TTTGAGGGGT TGAAGCCGTC AGCACCACTC
TCTTGGGGCT GGTTTGGAAC AGTCCGAGTG GACCGGCGAG TGGCTCGAGG AGAGGAGCAG
CAGCGGTTGC TGCTCTACCA CACACACCTG AGGCCCCGGC CCCGCGCCTA TTACCTGGAG
CCACTGCCAC TGCCCCCAGA AGATGAGGAG CCGCCTGCTC CTACCCTGCT AGAGCCTGAG
AAAAAGGCTC CAGAGCCCCC CAAAACTGAC AAACCGGGGG CTGCTCCACC CAGTACTGAG
GAACGCAAGA AGAAGTCCAC CAAGGGCAAG AAACGCAGCC AGCCAGCTAC CAAGACAGAG
GACTATGGAA TGGGCCCGGG TCGGAGCGGC CCTTATGGTG TGACAGTGCC TCCGGACCTC
CTGCACCACC CAAACCCTGG TTCTATAACA CACCTTAACT ACAGGCAAGG CTCCATAGGC
CTGTACACCC AGAACCAGCC ACTACCTGCA GGTGGCCCTC GTGTGGACCC ATACCGTCCT
GTGCGCTTAC CAATGCAGAA GCTGCCCACC CGACCAACTT ACCCTGGAGT GCTGCCCACA
ACCATGACTG GCGTCATGGG TTTAGAACCC TCCTCTTATA AGACCTCTGT GTACCGGCAG
CAGCAACCTG CGGTGCCCCA AGGACAGCGC CTTCGCCAAC AGCTCCAGCA GAGTCAGGGC
ATGTTGGGAC AGTCATCTGT CCATCAGATG ACTCCCAGCT CTTCCTACGG TTTGCAGACT
TCCCAGGGCT ATACTCCTTA TGTTTCTCAT GTGGGATTGC AGCAACACAC AGGCCCTGCA
GGTACCATGG TGCCCCCCAG CTACTCCAGC CAGCCTTACC AGAGCACCCA CCCTTCTACC
AATCCTACTC TTGTAGATCC TACCCGCCAC CTGCAACAGC GGCCCAGTGG CTATGTGCAC
CAGCAGGCCC CCACCTATGG ACATGGACTG ACCTCCACTC AAAGGTTTTC ACACCAGACA
CTGCAGCAGA CACCCATGAT AAGTACCATG ACTCCAATGA GTGCCCAGGG CGTCCAGGCA
GGCGTCCGTT CAACAGCCAT CCTACCTGAG CAGCAGCAGC AGCAGCAACA GCAGCAACAG
CAACAGCAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGTA CCACATCCGG
CAGCAGCAGC AGCAGCAGAT CCTGCGGCAG CAGCAGCAAC AGCAACAGCA GCAGCAGCAG
CAGCAGCAAC AGCAACAGCA GCAGCAGCAA CAGCAACAAC AGCAACACCA GCAGCAACAG
CAGCAACAGG CGGCTCCTCC CCAACCCCAG CCCCAGTCCC AGCCCCAGTT CCAGCGCCAG
GGGCTTCAGC AGACCCAGCA GCAGCAACAG ACAGCAGCTT TGGTCCGGCA ACTTCAACAA
CAGCTCTCTA ATACCCAGCC ACAGCCCAGT ACCAACATAT TTGGACGCTA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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