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SLC12A6 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SLC12A6
Entrez Gene ID 9990
Full Name solute carrier family 12 (potassium/chloride transporter), member 6
Synonyms ACCPN, KCC3, KCC3A, KCC3B
General protein information
Preferred Names
solute carrier family 12 member 6
Names
solute carrier family 12 member 6
K-Cl cotransporter 3
potassium-chloride transporter-3a
potassium-chloride transporter-3b
potassium chloride cotransporter 3
potassium chloride cotransporter KCC3a-S3
electroneutral potassium-chloride cotransporter 3
solute carrier family 12 (potassium/chloride transporters), member 6
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q13

Summary This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604878

Disorder Html: Agenesis of the corpus callosum with peripheral neuropathy, 218000

mRNA and Protein(s)

mRNA Protein Name
XM_011522267 XP_011520569 solute carrier family 12 member 6 isoform X1
XM_011522268 XP_011520570 solute carrier family 12 member 6 isoform X1
XM_006720793 XP_006720856 solute carrier family 12 member 6 isoform X2
XM_011522269 XP_011520571 solute carrier family 12 member 6 isoform X3
NM_133647 NP_598408 solute carrier family 12 member 6 isoform a
NM_005135 NP_005126 solute carrier family 12 member 6 isoform b
NM_001042494 NP_001035959 solute carrier family 12 member 6 isoform c
NM_001042495 NP_001035960 solute carrier family 12 member 6 isoform c
NM_001042496 NP_001035961 solute carrier family 12 member 6 isoform d
NM_001042497 NP_001035962 solute carrier family 12 member 6 isoform e



Homo sapiens (human) SLC12A6 NP_598408.1
Pan troglodytes (chimpanzee) SLC12A6 XP_001173480.1
Macaca mulatta (Rhesus monkey) SLC12A6 XP_002804750.1
Canis lupus familiaris (dog) SLC12A6 XP_005638240.1
Bos taurus (cattle) SLC12A6 NP_001180045.1
Mus musculus (house mouse) Slc12a6 NP_598410.2
Rattus norvegicus (Norway rat) Slc12a6 NP_001103100.1
Danio rerio (zebrafish) slc12a7b XP_696060.6
Xenopus (Silurana) tropicalis (western clawed frog) slc12a6 NP_001072576.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLC12A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu18029 XM_011522267 PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu18029 XM_011522268 PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu48703 XM_006720793 PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu63583 XM_011522269 PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu18029 NM_133647 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu18033 NM_005135 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $776.30
$1109.00
OHu16186 NM_001042494 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu16186 NM_001042495 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00
OHu16155 NM_001042496 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $811.30
$1159.00
OHu16248 NM_001042497 Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $797.30
$1139.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18029
Clone ID Related Accession (Same CDS sequence) NM_133647 , XM_011522267 , XM_011522268
Accession Version XM_011522267.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGGGCCCTTT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
TATTATGCCA TTGTAGCCAT GGTAATAGCT GGTATGATCT ACAAGTACAT TGAATACCAA
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGTGGGCTGT CCCTCAGTGC AGCCCGGTTT
GCTTTGCTTC GATTGGAGGA AGGACCTCCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
GTATTACTGA AACTAGATGA AGACTTACAT GTCAAGCATC CTCGCCTCCT CACCTTTGCC
TCACAGCTCA AAGCAGGAAA AGGTCTCACT ATTGTGGGCT CTGTCATCGT GGGGAACTTC
CTAGAGAACT ACGGTGAAGC TTTAGCTGCT GAGCAGACCA TAAAGCACCT AATGGAGGCA
GAGAAGGTAA AAGGATTCTG CCAGCTGGTG GTGGCCGCCA AGCTGAGAGA GGGCATTTCC
CACCTCATCC AGTCATGTGG CCTTGGGGGC ATGAAGCACA ACACGGTGGT GATGGGCTGG
CCTAATGGCT GGCGTCAAAG CGAAGATGCC CGCGCTTGGA AGACTTTTAT TGGCACAGTT
CGAGTGACAA CTGCTGCCCA TCTTGCACTG CTGGTGGCTA AAAACATCTC CTTCTTTCCC
AGCAATGTGG AGCAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG
GGGATGCTTA TGCTACTACC ATTCCTACTG AAACAGCACA AGGTGTGGCG AAAGTGCAGC
ATACGGATCT TCACAGTAGC CCAATTAGAA GACAACAGTA TCCAAATGAA GAAGGACCTA
GCCACCTTCC TATATCACTT ACGCATTGAG GCGGAGGTAG AAGTGGTGGA GATGCATGAC
AGTGATATAT CAGCATATAC TTACGAGCGC ACTTTGATGA TGGAACAAAG GTCCCAGATG
CTCCGGCACA TGCGGCTATC CAAAACAGAG CGAGACAGAG AGGCACAATT GGTGAAAGAC
CGAAACTCAA TGCTACGATT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACC
TATCAGGAGA AGGTGCACAT GACTTGGACA AAAGACAAGT ACATGGCATC CCGGGGACAA
AAAGCGAAGT CAATGGAAGG ATTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
GTGAGGCGGA TGCATACAGC AGTGAAACTC AACGAGGTTA TAGTTAACAA GTCCCATGAA
GCAAAGCTGG TTTTATTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGATGAAAAC
TACATGGAGT TCCTAGAGGT GCTTACCGAG GGACTAGAGC GAGTCCTACT TGTCCGGGGT
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520569.1
CDS481..3933
Misc Feature(1)805..3930(+)
Misc Feature(2)1105..>1560(+)
Misc Feature(3)2023..2619(+)
Misc Feature(4)3532..3621(+)
Translation

Target ORF information:

RefSeq Version XM_011522267
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522267

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGGGCCCTTT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
TATTATGCCA TTGTAGCCAT GGTAATAGCT GGTATGATCT ACAAGTACAT TGAATACCAA
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGTGGGCTGT CCCTCAGTGC AGCCCGGTTT
GCTTTGCTTC GATTGGAGGA AGGACCTCCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
GTATTACTGA AACTAGATGA AGACTTACAT GTCAAGCATC CTCGCCTCCT CACCTTTGCC
TCACAGCTCA AAGCAGGAAA AGGTCTCACT ATTGTGGGCT CTGTCATCGT GGGGAACTTC
CTAGAGAACT ACGGTGAAGC TTTAGCTGCT GAGCAGACCA TAAAGCACCT AATGGAGGCA
GAGAAGGTAA AAGGATTCTG CCAGCTGGTG GTGGCCGCCA AGCTGAGAGA GGGCATTTCC
CACCTCATCC AGTCATGTGG CCTTGGGGGC ATGAAGCACA ACACGGTGGT GATGGGCTGG
CCTAATGGCT GGCGTCAAAG CGAAGATGCC CGCGCTTGGA AGACTTTTAT TGGCACAGTT
CGAGTGACAA CTGCTGCCCA TCTTGCACTG CTGGTGGCTA AAAACATCTC CTTCTTTCCC
AGCAATGTGG AGCAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG
GGGATGCTTA TGCTACTACC ATTCCTACTG AAACAGCACA AGGTGTGGCG AAAGTGCAGC
ATACGGATCT TCACAGTAGC CCAATTAGAA GACAACAGTA TCCAAATGAA GAAGGACCTA
GCCACCTTCC TATATCACTT ACGCATTGAG GCGGAGGTAG AAGTGGTGGA GATGCATGAC
AGTGATATAT CAGCATATAC TTACGAGCGC ACTTTGATGA TGGAACAAAG GTCCCAGATG
CTCCGGCACA TGCGGCTATC CAAAACAGAG CGAGACAGAG AGGCACAATT GGTGAAAGAC
CGAAACTCAA TGCTACGATT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACC
TATCAGGAGA AGGTGCACAT GACTTGGACA AAAGACAAGT ACATGGCATC CCGGGGACAA
AAAGCGAAGT CAATGGAAGG ATTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
GTGAGGCGGA TGCATACAGC AGTGAAACTC AACGAGGTTA TAGTTAACAA GTCCCATGAA
GCAAAGCTGG TTTTATTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGATGAAAAC
TACATGGAGT TCCTAGAGGT GCTTACCGAG GGACTAGAGC GAGTCCTACT TGTCCGGGGT
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18029
Clone ID Related Accession (Same CDS sequence) NM_133647 , XM_011522267 , XM_011522268
Accession Version XM_011522268.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGGGCCCTTT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
TATTATGCCA TTGTAGCCAT GGTAATAGCT GGTATGATCT ACAAGTACAT TGAATACCAA
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGTGGGCTGT CCCTCAGTGC AGCCCGGTTT
GCTTTGCTTC GATTGGAGGA AGGACCTCCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
GTATTACTGA AACTAGATGA AGACTTACAT GTCAAGCATC CTCGCCTCCT CACCTTTGCC
TCACAGCTCA AAGCAGGAAA AGGTCTCACT ATTGTGGGCT CTGTCATCGT GGGGAACTTC
CTAGAGAACT ACGGTGAAGC TTTAGCTGCT GAGCAGACCA TAAAGCACCT AATGGAGGCA
GAGAAGGTAA AAGGATTCTG CCAGCTGGTG GTGGCCGCCA AGCTGAGAGA GGGCATTTCC
CACCTCATCC AGTCATGTGG CCTTGGGGGC ATGAAGCACA ACACGGTGGT GATGGGCTGG
CCTAATGGCT GGCGTCAAAG CGAAGATGCC CGCGCTTGGA AGACTTTTAT TGGCACAGTT
CGAGTGACAA CTGCTGCCCA TCTTGCACTG CTGGTGGCTA AAAACATCTC CTTCTTTCCC
AGCAATGTGG AGCAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG
GGGATGCTTA TGCTACTACC ATTCCTACTG AAACAGCACA AGGTGTGGCG AAAGTGCAGC
ATACGGATCT TCACAGTAGC CCAATTAGAA GACAACAGTA TCCAAATGAA GAAGGACCTA
GCCACCTTCC TATATCACTT ACGCATTGAG GCGGAGGTAG AAGTGGTGGA GATGCATGAC
AGTGATATAT CAGCATATAC TTACGAGCGC ACTTTGATGA TGGAACAAAG GTCCCAGATG
CTCCGGCACA TGCGGCTATC CAAAACAGAG CGAGACAGAG AGGCACAATT GGTGAAAGAC
CGAAACTCAA TGCTACGATT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACC
TATCAGGAGA AGGTGCACAT GACTTGGACA AAAGACAAGT ACATGGCATC CCGGGGACAA
AAAGCGAAGT CAATGGAAGG ATTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
GTGAGGCGGA TGCATACAGC AGTGAAACTC AACGAGGTTA TAGTTAACAA GTCCCATGAA
GCAAAGCTGG TTTTATTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGATGAAAAC
TACATGGAGT TCCTAGAGGT GCTTACCGAG GGACTAGAGC GAGTCCTACT TGTCCGGGGT
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520570.1
CDS170..3622
Misc Feature(1)494..3619(+)
Misc Feature(2)794..>1249(+)
Misc Feature(3)1712..2308(+)
Misc Feature(4)3221..3310(+)
Translation

Target ORF information:

RefSeq Version XM_011522268
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522268

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGGGCCCTTT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
TATTATGCCA TTGTAGCCAT GGTAATAGCT GGTATGATCT ACAAGTACAT TGAATACCAA
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGTGGGCTGT CCCTCAGTGC AGCCCGGTTT
GCTTTGCTTC GATTGGAGGA AGGACCTCCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
GTATTACTGA AACTAGATGA AGACTTACAT GTCAAGCATC CTCGCCTCCT CACCTTTGCC
TCACAGCTCA AAGCAGGAAA AGGTCTCACT ATTGTGGGCT CTGTCATCGT GGGGAACTTC
CTAGAGAACT ACGGTGAAGC TTTAGCTGCT GAGCAGACCA TAAAGCACCT AATGGAGGCA
GAGAAGGTAA AAGGATTCTG CCAGCTGGTG GTGGCCGCCA AGCTGAGAGA GGGCATTTCC
CACCTCATCC AGTCATGTGG CCTTGGGGGC ATGAAGCACA ACACGGTGGT GATGGGCTGG
CCTAATGGCT GGCGTCAAAG CGAAGATGCC CGCGCTTGGA AGACTTTTAT TGGCACAGTT
CGAGTGACAA CTGCTGCCCA TCTTGCACTG CTGGTGGCTA AAAACATCTC CTTCTTTCCC
AGCAATGTGG AGCAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG
GGGATGCTTA TGCTACTACC ATTCCTACTG AAACAGCACA AGGTGTGGCG AAAGTGCAGC
ATACGGATCT TCACAGTAGC CCAATTAGAA GACAACAGTA TCCAAATGAA GAAGGACCTA
GCCACCTTCC TATATCACTT ACGCATTGAG GCGGAGGTAG AAGTGGTGGA GATGCATGAC
AGTGATATAT CAGCATATAC TTACGAGCGC ACTTTGATGA TGGAACAAAG GTCCCAGATG
CTCCGGCACA TGCGGCTATC CAAAACAGAG CGAGACAGAG AGGCACAATT GGTGAAAGAC
CGAAACTCAA TGCTACGATT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACC
TATCAGGAGA AGGTGCACAT GACTTGGACA AAAGACAAGT ACATGGCATC CCGGGGACAA
AAAGCGAAGT CAATGGAAGG ATTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
GTGAGGCGGA TGCATACAGC AGTGAAACTC AACGAGGTTA TAGTTAACAA GTCCCATGAA
GCAAAGCTGG TTTTATTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGATGAAAAC
TACATGGAGT TCCTAGAGGT GCTTACCGAG GGACTAGAGC GAGTCCTACT TGTCCGGGGT
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48703
Clone ID Related Accession (Same CDS sequence) XM_006720793
Accession Version XM_006720793.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578827667. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACAATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
GGGGGCTCAT ACTTTATGAT TTCCCGGGCA CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC
CTCTGCTTTT ATCTTGGTAC CACATTTGCA GCAGCCATGT ACATCCTTGG TGCCATTGAA
ATCTTTCTGG TCTATATCGT CCCCCGAGCT GCCATCTTTC ACAGTGATGA CGCACTCAAG
GAATCAGCAG CCATGCTAAA TAACATGCGT GTCTACGGCA CAGCTTTCTT GGTCCTTATG
GTATTAGTGG TATTTATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT TTTCCTGGCC
TGTGTCATTG TGTCCATCTT GGCCATCTAT GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT
CCACACTTCC CGGTCTGCAT GCTGGGTAAC CGCACCCTTT CATCAAGACA CATTGACGTT
TGCTCTAAGA CCAAGGAAAT TAACAACATG ACAGTCCCAT CAAAGTTATG GGGATTCTTC
TGTAACTCGA GTCAATTTTT CAATGCCACC TGTGATGAAT ACTTTGTTCA CAATAACGTC
ACTTCAATCC AGGGCATTCC TGGATTGGCT AGTGGTATAA TTACAGAGAA TCTTTGGAGT
AATTACCTAC CCAAGGGAGA GATCATCGAA AAGCCTTCAG CCAAATCTTC TGATGTCTTA
GGCAGCTTAA ACCATGAATA TGTTCTTGTT GACATCACCA CCTCCTTCAC GCTTCTGGTG
GGAATCTTCT TTCCCTCTGT TACAGGTATC ATGGCTGGAT CAAACAGATC TGGAGATCTG
AAAGATGCTC AGAAGTCTAT TCCGATTGGT ACTATCCTTG CCATCCTGAC CACCTCCTTT
GTTTATTTAA GCAATGTTGT CCTTTTTGGT GCATGTATTG AAGGGGTTGT TCTCAGAGAC
AAGTTCGGTG ATGCTGTGAA AGGTAATTTG GTGGTAGGCA CCTTATCTTG GCCATCCCCA
TGGGTGATTG TTATTGGCTC CTTCTTTTCA ACATGTGGGG CTGGACTTCA GAGCCTCACA
GGTGCACCGA GGCTGCTACA AGCTATTGCC AAGGATAACA TCATACCGTT TCTGAGGGTT
TTTGGCCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTAAC TGCTGCCATT
GCAGAGCTTG GAATACTCAT TGCCTCCCTG GATCTTGTGG CCCCAATTCT TTCCATGTTT
TTTCTCATGT GTTACCTCTT TGTAAACTTG GCATGTGCCT TGCAAACATT ACTTCGAACA
CCCAACTGGA GACCCCGATT CCGCTACTAC CATTGGGCCC TTTCTTTCAT GGGAATGAGT
ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATTGTAGC CATGGTAATA
GCTGGTATGA TCTACAAGTA CATTGAATAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG TTTGCTTTGC TTCGATTGGA GGAAGGACCT
CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTAC TGAAACTAGA TGAAGACTTA
CATGTCAAGC ATCCTCGCCT CCTCACCTTT GCCTCACAGC TCAAAGCAGG AAAAGGTCTC
ACTATTGTGG GCTCTGTCAT CGTGGGGAAC TTCCTAGAGA ACTACGGTGA AGCTTTAGCT
GCTGAGCAGA CCATAAAGCA CCTAATGGAG GCAGAGAAGG TAAAAGGATT CTGCCAGCTG
GTGGTGGCCG CCAAGCTGAG AGAGGGCATT TCCCACCTCA TCCAGTCATG TGGCCTTGGG
GGCATGAAGC ACAACACGGT GGTGATGGGC TGGCCTAATG GCTGGCGTCA AAGCGAAGAT
GCCCGCGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTTGCA
CTGCTGGTGG CTAAAAACAT CTCCTTCTTT CCCAGCAATG TGGAGCAATT TTCTGAGGGC
AACATTGATG TGTGGTGGAT TGTGCATGAT GGGGGGATGC TTATGCTACT ACCATTCCTA
CTGAAACAGC ACAAGGTGTG GCGAAAGTGC AGCATACGGA TCTTCACAGT AGCCCAATTA
GAAGACAACA GTATCCAAAT GAAGAAGGAC CTAGCCACCT TCCTATATCA CTTACGCATT
GAGGCGGAGG TAGAAGTGGT GGAGATGCAT GACAGTGATA TATCAGCATA TACTTACGAG
CGCACTTTGA TGATGGAACA AAGGTCCCAG ATGCTCCGGC ACATGCGGCT ATCCAAAACA
GAGCGAGACA GAGAGGCACA ATTGGTGAAA GACCGAAACT CAATGCTACG ATTGACCAGC
ATTGGCTCTG ATGAGGACGA AGAGACAGAA ACCTATCAGG AGAAGGTGCA CATGACTTGG
ACAAAAGACA AGTACATGGC ATCCCGGGGA CAAAAAGCGA AGTCAATGGA AGGATTCCAG
GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAA
CTCAACGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTATT GAATATGCCA
GGGCCACCCC GAAACCCTGA GGGTGATGAA AACTACATGG AGTTCCTAGA GGTGCTTACC
GAGGGACTAG AGCGAGTCCT ACTTGTCCGG GGTGGTGGCA GTGAAGTGAT CACCATTTAT
TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006720856.1
CDS7..3312
Misc Feature(1)331..3309(+)
Misc Feature(2)556..>939(+)
Misc Feature(3)1402..1998(+)
Misc Feature(4)2911..3000(+)
Translation

Target ORF information:

RefSeq Version XM_006720793
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006720793

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACAATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
GGGGGCTCAT ACTTTATGAT TTCCCGGGCA CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC
CTCTGCTTTT ATCTTGGTAC CACATTTGCA GCAGCCATGT ACATCCTTGG TGCCATTGAA
ATCTTTCTGG TCTATATCGT CCCCCGAGCT GCCATCTTTC ACAGTGATGA CGCACTCAAG
GAATCAGCAG CCATGCTAAA TAACATGCGT GTCTACGGCA CAGCTTTCTT GGTCCTTATG
GTATTAGTGG TATTTATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT TTTCCTGGCC
TGTGTCATTG TGTCCATCTT GGCCATCTAT GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT
CCACACTTCC CGGTCTGCAT GCTGGGTAAC CGCACCCTTT CATCAAGACA CATTGACGTT
TGCTCTAAGA CCAAGGAAAT TAACAACATG ACAGTCCCAT CAAAGTTATG GGGATTCTTC
TGTAACTCGA GTCAATTTTT CAATGCCACC TGTGATGAAT ACTTTGTTCA CAATAACGTC
ACTTCAATCC AGGGCATTCC TGGATTGGCT AGTGGTATAA TTACAGAGAA TCTTTGGAGT
AATTACCTAC CCAAGGGAGA GATCATCGAA AAGCCTTCAG CCAAATCTTC TGATGTCTTA
GGCAGCTTAA ACCATGAATA TGTTCTTGTT GACATCACCA CCTCCTTCAC GCTTCTGGTG
GGAATCTTCT TTCCCTCTGT TACAGGTATC ATGGCTGGAT CAAACAGATC TGGAGATCTG
AAAGATGCTC AGAAGTCTAT TCCGATTGGT ACTATCCTTG CCATCCTGAC CACCTCCTTT
GTTTATTTAA GCAATGTTGT CCTTTTTGGT GCATGTATTG AAGGGGTTGT TCTCAGAGAC
AAGTTCGGTG ATGCTGTGAA AGGTAATTTG GTGGTAGGCA CCTTATCTTG GCCATCCCCA
TGGGTGATTG TTATTGGCTC CTTCTTTTCA ACATGTGGGG CTGGACTTCA GAGCCTCACA
GGTGCACCGA GGCTGCTACA AGCTATTGCC AAGGATAACA TCATACCGTT TCTGAGGGTT
TTTGGCCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTAAC TGCTGCCATT
GCAGAGCTTG GAATACTCAT TGCCTCCCTG GATCTTGTGG CCCCAATTCT TTCCATGTTT
TTTCTCATGT GTTACCTCTT TGTAAACTTG GCATGTGCCT TGCAAACATT ACTTCGAACA
CCCAACTGGA GACCCCGATT CCGCTACTAC CATTGGGCCC TTTCTTTCAT GGGAATGAGT
ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATTGTAGC CATGGTAATA
GCTGGTATGA TCTACAAGTA CATTGAATAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG TTTGCTTTGC TTCGATTGGA GGAAGGACCT
CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTAC TGAAACTAGA TGAAGACTTA
CATGTCAAGC ATCCTCGCCT CCTCACCTTT GCCTCACAGC TCAAAGCAGG AAAAGGTCTC
ACTATTGTGG GCTCTGTCAT CGTGGGGAAC TTCCTAGAGA ACTACGGTGA AGCTTTAGCT
GCTGAGCAGA CCATAAAGCA CCTAATGGAG GCAGAGAAGG TAAAAGGATT CTGCCAGCTG
GTGGTGGCCG CCAAGCTGAG AGAGGGCATT TCCCACCTCA TCCAGTCATG TGGCCTTGGG
GGCATGAAGC ACAACACGGT GGTGATGGGC TGGCCTAATG GCTGGCGTCA AAGCGAAGAT
GCCCGCGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTTGCA
CTGCTGGTGG CTAAAAACAT CTCCTTCTTT CCCAGCAATG TGGAGCAATT TTCTGAGGGC
AACATTGATG TGTGGTGGAT TGTGCATGAT GGGGGGATGC TTATGCTACT ACCATTCCTA
CTGAAACAGC ACAAGGTGTG GCGAAAGTGC AGCATACGGA TCTTCACAGT AGCCCAATTA
GAAGACAACA GTATCCAAAT GAAGAAGGAC CTAGCCACCT TCCTATATCA CTTACGCATT
GAGGCGGAGG TAGAAGTGGT GGAGATGCAT GACAGTGATA TATCAGCATA TACTTACGAG
CGCACTTTGA TGATGGAACA AAGGTCCCAG ATGCTCCGGC ACATGCGGCT ATCCAAAACA
GAGCGAGACA GAGAGGCACA ATTGGTGAAA GACCGAAACT CAATGCTACG ATTGACCAGC
ATTGGCTCTG ATGAGGACGA AGAGACAGAA ACCTATCAGG AGAAGGTGCA CATGACTTGG
ACAAAAGACA AGTACATGGC ATCCCGGGGA CAAAAAGCGA AGTCAATGGA AGGATTCCAG
GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAA
CTCAACGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTATT GAATATGCCA
GGGCCACCCC GAAACCCTGA GGGTGATGAA AACTACATGG AGTTCCTAGA GGTGCTTACC
GAGGGACTAG AGCGAGTCCT ACTTGTCCGG GGTGGTGGCA GTGAAGTGAT CACCATTTAT
TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu63583
Clone ID Related Accession (Same CDS sequence) XM_011522269
Accession Version XM_011522269.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2043bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520571.1
CDS7..2049
Misc Feature(1)331..>2046(+)
Misc Feature(2)631..>1086(+)
Translation

Target ORF information:

RefSeq Version XM_011522269
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522269

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18029
Clone ID Related Accession (Same CDS sequence) NM_133647 , XM_011522267 , XM_011522268
Accession Version NM_133647.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF105366.1 and AC079203.9. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF105366.1, AF116242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGGGCCCTTT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
TATTATGCCA TTGTAGCCAT GGTAATAGCT GGTATGATCT ACAAGTACAT TGAATACCAA
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGTGGGCTGT CCCTCAGTGC AGCCCGGTTT
GCTTTGCTTC GATTGGAGGA AGGACCTCCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
GTATTACTGA AACTAGATGA AGACTTACAT GTCAAGCATC CTCGCCTCCT CACCTTTGCC
TCACAGCTCA AAGCAGGAAA AGGTCTCACT ATTGTGGGCT CTGTCATCGT GGGGAACTTC
CTAGAGAACT ACGGTGAAGC TTTAGCTGCT GAGCAGACCA TAAAGCACCT AATGGAGGCA
GAGAAGGTAA AAGGATTCTG CCAGCTGGTG GTGGCCGCCA AGCTGAGAGA GGGCATTTCC
CACCTCATCC AGTCATGTGG CCTTGGGGGC ATGAAGCACA ACACGGTGGT GATGGGCTGG
CCTAATGGCT GGCGTCAAAG CGAAGATGCC CGCGCTTGGA AGACTTTTAT TGGCACAGTT
CGAGTGACAA CTGCTGCCCA TCTTGCACTG CTGGTGGCTA AAAACATCTC CTTCTTTCCC
AGCAATGTGG AGCAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG
GGGATGCTTA TGCTACTACC ATTCCTACTG AAACAGCACA AGGTGTGGCG AAAGTGCAGC
ATACGGATCT TCACAGTAGC CCAATTAGAA GACAACAGTA TCCAAATGAA GAAGGACCTA
GCCACCTTCC TATATCACTT ACGCATTGAG GCGGAGGTAG AAGTGGTGGA GATGCATGAC
AGTGATATAT CAGCATATAC TTACGAGCGC ACTTTGATGA TGGAACAAAG GTCCCAGATG
CTCCGGCACA TGCGGCTATC CAAAACAGAG CGAGACAGAG AGGCACAATT GGTGAAAGAC
CGAAACTCAA TGCTACGATT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACC
TATCAGGAGA AGGTGCACAT GACTTGGACA AAAGACAAGT ACATGGCATC CCGGGGACAA
AAAGCGAAGT CAATGGAAGG ATTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
GTGAGGCGGA TGCATACAGC AGTGAAACTC AACGAGGTTA TAGTTAACAA GTCCCATGAA
GCAAAGCTGG TTTTATTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGATGAAAAC
TACATGGAGT TCCTAGAGGT GCTTACCGAG GGACTAGAGC GAGTCCTACT TGTCCGGGGT
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_598408.1
CDS165..3617
Misc Feature(1)54..56(+)
Misc Feature(2)258..260(+)
Misc Feature(3)489..3614(+)
Misc Feature(4)720..788(+)
Misc Feature(5)789..>1244(+)
Misc Feature(6)834..899(+)
Misc Feature(7)978..1049(+)
Misc Feature(8)1119..1193(+)
Misc Feature(9)1203..1277(+)
Misc Feature(10)1602..1667(+)
Misc Feature(11)1707..2303(+)
Misc Feature(12)1710..1793(+)
Misc Feature(13)1836..1907(+)
Misc Feature(14)2025..2096(+)
Misc Feature(15)2106..2174(+)
Misc Feature(16)2193..2249(+)
Misc Feature(17)2253..2321(+)
Misc Feature(18)3216..3305(+)
Misc Feature(19)3258..3260(+)
Exon (1)1..435
Gene:SLC12A6
Gene Synonym:
Exon (2)436..480
Gene:SLC12A6
Gene Synonym:
Exon (3)481..575
Gene:SLC12A6
Gene Synonym:
Exon (4)576..707
Gene:SLC12A6
Gene Synonym:
Exon (5)708..854
Gene:SLC12A6
Gene Synonym:
Exon (6)855..909
Gene:SLC12A6
Gene Synonym:
Exon (7)910..1040
Gene:SLC12A6
Gene Synonym:
Exon (8)1041..1282
Gene:SLC12A6
Gene Synonym:
Exon (9)1283..1497
Gene:SLC12A6
Gene Synonym:
Exon (10)1498..1656
Gene:SLC12A6
Gene Synonym:
Exon (11)1657..1755
Gene:SLC12A6
Gene Synonym:
Exon (12)1756..1813
Gene:SLC12A6
Gene Synonym:
Exon (13)1814..1988
Gene:SLC12A6
Gene Synonym:
Exon (14)1989..2107
Gene:SLC12A6
Gene Synonym:
Exon (15)2108..2206
Gene:SLC12A6
Gene Synonym:
Exon (16)2207..2326
Gene:SLC12A6
Gene Synonym:
Exon (17)2327..2431
Gene:SLC12A6
Gene Synonym:
Exon (18)2432..2600
Gene:SLC12A6
Gene Synonym:
Exon (19)2601..2796
Gene:SLC12A6
Gene Synonym:
Exon (20)2797..2966
Gene:SLC12A6
Gene Synonym:
Exon (21)2967..3098
Gene:SLC12A6
Gene Synonym:
Exon (22)3099..3206
Gene:SLC12A6
Gene Synonym:
Exon (23)3207..3391
Gene:SLC12A6
Gene Synonym:
Exon (24)3392..3525
Gene:SLC12A6
Gene Synonym:
Exon (25)3526..7502
Gene:SLC12A6
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_133647
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_133647

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACCTGAGTC AGAACTCCAT CACAGGGGAA
CACAGCCAAC TGTTAGACGA CGGACATAAG AAAGCTCGAA ATGCTTATCT CAATAATTCC
AATTATGAAG AAGGAGATGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG
GACACCAGAC CGAAGGTGTC TTCCCTCCTC AACCGCATGG CCAATTACAC TAATCTGACT
CAAGGAGCAA AGGAACATGA AGAGGCAGAA AACATCACTG AAGGGAAAAA GAAGCCCACC
AAGACCCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
GGAGTGATCC TTTTTTTACG CCTTACATGG GTGGTGGGCA CAGCTGGAGT TCTTCAGGCT
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACAATGTTGA CTGCTATCTC CATGAGTGCC
ATTGCCACTA ATGGAGTGGT GCCAGCTGGG GGCTCATACT TTATGATTTC CCGGGCACTG
GGCCCAGAGT TTGGTGGGGC TGTTGGCCTC TGCTTTTATC TTGGTACCAC ATTTGCAGCA
GCCATGTACA TCCTTGGTGC CATTGAAATC TTTCTGGTCT ATATCGTCCC CCGAGCTGCC
ATCTTTCACA GTGATGACGC ACTCAAGGAA TCAGCAGCCA TGCTAAATAA CATGCGTGTC
TACGGCACAG CTTTCTTGGT CCTTATGGTA TTAGTGGTAT TTATCGGCGT ACGCTATGTG
AACAAGTTTG CCTCACTTTT CCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTATGCT
GGAGCCATCA AGTCTTCTTT TGCTCCTCCA CACTTCCCGG TCTGCATGCT GGGTAACCGC
ACCCTTTCAT CAAGACACAT TGACGTTTGC TCTAAGACCA AGGAAATTAA CAACATGACA
GTCCCATCAA AGTTATGGGG ATTCTTCTGT AACTCGAGTC AATTTTTCAA TGCCACCTGT
GATGAATACT TTGTTCACAA TAACGTCACT TCAATCCAGG GCATTCCTGG ATTGGCTAGT
GGTATAATTA CAGAGAATCT TTGGAGTAAT TACCTACCCA AGGGAGAGAT CATCGAAAAG
CCTTCAGCCA AATCTTCTGA TGTCTTAGGC AGCTTAAACC ATGAATATGT TCTTGTTGAC
ATCACCACCT CCTTCACGCT TCTGGTGGGA ATCTTCTTTC CCTCTGTTAC AGGTATCATG
GCTGGATCAA ACAGATCTGG AGATCTGAAA GATGCTCAGA AGTCTATTCC GATTGGTACT
ATCCTTGCCA TCCTGACCAC CTCCTTTGTT TATTTAAGCA ATGTTGTCCT TTTTGGTGCA
TGTATTGAAG GGGTTGTTCT CAGAGACAAG TTCGGTGATG CTGTGAAAGG TAATTTGGTG
GTAGGCACCT TATCTTGGCC ATCCCCATGG GTGATTGTTA TTGGCTCCTT CTTTTCAACA
TGTGGGGCTG GACTTCAGAG CCTCACAGGT GCACCGAGGC TGCTACAAGC TATTGCCAAG
GATAACATCA TACCGTTTCT GAGGGTTTTT GGCCACAGCA AAGCCAATGG GGAACCTACC
TGGGCTTTAC TTCTAACTGC TGCCATTGCA GAGCTTGGAA TACTCATTGC CTCCCTGGAT
CTTGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT AAACTTGGCA
TGTGCCTTGC AAACATTACT TCGAACACCC AACTGGAGAC CCCGATTCCG CTACTACCAT
TGGGCCCTTT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
TATTATGCCA TTGTAGCCAT GGTAATAGCT GGTATGATCT ACAAGTACAT TGAATACCAA
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGTGGGCTGT CCCTCAGTGC AGCCCGGTTT
GCTTTGCTTC GATTGGAGGA AGGACCTCCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
GTATTACTGA AACTAGATGA AGACTTACAT GTCAAGCATC CTCGCCTCCT CACCTTTGCC
TCACAGCTCA AAGCAGGAAA AGGTCTCACT ATTGTGGGCT CTGTCATCGT GGGGAACTTC
CTAGAGAACT ACGGTGAAGC TTTAGCTGCT GAGCAGACCA TAAAGCACCT AATGGAGGCA
GAGAAGGTAA AAGGATTCTG CCAGCTGGTG GTGGCCGCCA AGCTGAGAGA GGGCATTTCC
CACCTCATCC AGTCATGTGG CCTTGGGGGC ATGAAGCACA ACACGGTGGT GATGGGCTGG
CCTAATGGCT GGCGTCAAAG CGAAGATGCC CGCGCTTGGA AGACTTTTAT TGGCACAGTT
CGAGTGACAA CTGCTGCCCA TCTTGCACTG CTGGTGGCTA AAAACATCTC CTTCTTTCCC
AGCAATGTGG AGCAATTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG
GGGATGCTTA TGCTACTACC ATTCCTACTG AAACAGCACA AGGTGTGGCG AAAGTGCAGC
ATACGGATCT TCACAGTAGC CCAATTAGAA GACAACAGTA TCCAAATGAA GAAGGACCTA
GCCACCTTCC TATATCACTT ACGCATTGAG GCGGAGGTAG AAGTGGTGGA GATGCATGAC
AGTGATATAT CAGCATATAC TTACGAGCGC ACTTTGATGA TGGAACAAAG GTCCCAGATG
CTCCGGCACA TGCGGCTATC CAAAACAGAG CGAGACAGAG AGGCACAATT GGTGAAAGAC
CGAAACTCAA TGCTACGATT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACC
TATCAGGAGA AGGTGCACAT GACTTGGACA AAAGACAAGT ACATGGCATC CCGGGGACAA
AAAGCGAAGT CAATGGAAGG ATTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
GTGAGGCGGA TGCATACAGC AGTGAAACTC AACGAGGTTA TAGTTAACAA GTCCCATGAA
GCAAAGCTGG TTTTATTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGATGAAAAC
TACATGGAGT TCCTAGAGGT GCTTACCGAG GGACTAGAGC GAGTCCTACT TGTCCGGGGT
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18033
Clone ID Related Accession (Same CDS sequence) NM_005135
Accession Version NM_005135.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021822.21, AF108831.1 and AC079203.9. This sequence is a reference standard in the RefSeqGene project. On Jul 6, 2006 this sequence version replaced gi:4826779. Transcript Variant: This variant (2) uses an alternate exon for the 5' UTR and 5' coding region, compared to variant 1. The encoded protein (isoform b) is shorter and has a distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF108831.1, BC126241.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCCACATT TTACTGTGAC TAAGGTAGAG GACCCAGAGG AGGGGGCAGC GGCTTCGATC 
TCTCAAGAGC CTAGTTTAGC AGACATAAAA GCCCGGATTC AGGATTCAGA TGAACCAGAC
CTGAGTCAGA ACTCCATCAC AGGGGAACAC AGCCAACTGT TAGACGACGG ACATAAGAAA
GCTCGAAATG CTTATCTCAA TAATTCCAAT TATGAAGAAG GAGATGAATA TTTTGATAAA
AATTTGGCAC TCTTTGAGGA AGAAATGGAC ACCAGACCGA AGGTGTCTTC CCTCCTCAAC
CGCATGGCCA ATTACACTAA TCTGACTCAA GGAGCAAAGG AACATGAAGA GGCAGAAAAC
ATCACTGAAG GGAAAAAGAA GCCCACCAAG ACCCCCCAAA TGGGTACCTT CATGGGTGTC
TACCTCCCAT GTCTACAAAA TATTTTTGGA GTGATCCTTT TTTTACGCCT TACATGGGTG
GTGGGCACAG CTGGAGTTCT TCAGGCTTTT GCAATTGTCC TTATCTGCTG CTGCTGTACA
ATGTTGACTG CTATCTCCAT GAGTGCCATT GCCACTAATG GAGTGGTGCC AGCTGGGGGC
TCATACTTTA TGATTTCCCG GGCACTGGGC CCAGAGTTTG GTGGGGCTGT TGGCCTCTGC
TTTTATCTTG GTACCACATT TGCAGCAGCC ATGTACATCC TTGGTGCCAT TGAAATCTTT
CTGGTCTATA TCGTCCCCCG AGCTGCCATC TTTCACAGTG ATGACGCACT CAAGGAATCA
GCAGCCATGC TAAATAACAT GCGTGTCTAC GGCACAGCTT TCTTGGTCCT TATGGTATTA
GTGGTATTTA TCGGCGTACG CTATGTGAAC AAGTTTGCCT CACTTTTCCT GGCCTGTGTC
ATTGTGTCCA TCTTGGCCAT CTATGCTGGA GCCATCAAGT CTTCTTTTGC TCCTCCACAC
TTCCCGGTCT GCATGCTGGG TAACCGCACC CTTTCATCAA GACACATTGA CGTTTGCTCT
AAGACCAAGG AAATTAACAA CATGACAGTC CCATCAAAGT TATGGGGATT CTTCTGTAAC
TCGAGTCAAT TTTTCAATGC CACCTGTGAT GAATACTTTG TTCACAATAA CGTCACTTCA
ATCCAGGGCA TTCCTGGATT GGCTAGTGGT ATAATTACAG AGAATCTTTG GAGTAATTAC
CTACCCAAGG GAGAGATCAT CGAAAAGCCT TCAGCCAAAT CTTCTGATGT CTTAGGCAGC
TTAAACCATG AATATGTTCT TGTTGACATC ACCACCTCCT TCACGCTTCT GGTGGGAATC
TTCTTTCCCT CTGTTACAGG TATCATGGCT GGATCAAACA GATCTGGAGA TCTGAAAGAT
GCTCAGAAGT CTATTCCGAT TGGTACTATC CTTGCCATCC TGACCACCTC CTTTGTTTAT
TTAAGCAATG TTGTCCTTTT TGGTGCATGT ATTGAAGGGG TTGTTCTCAG AGACAAGTTC
GGTGATGCTG TGAAAGGTAA TTTGGTGGTA GGCACCTTAT CTTGGCCATC CCCATGGGTG
ATTGTTATTG GCTCCTTCTT TTCAACATGT GGGGCTGGAC TTCAGAGCCT CACAGGTGCA
CCGAGGCTGC TACAAGCTAT TGCCAAGGAT AACATCATAC CGTTTCTGAG GGTTTTTGGC
CACAGCAAAG CCAATGGGGA ACCTACCTGG GCTTTACTTC TAACTGCTGC CATTGCAGAG
CTTGGAATAC TCATTGCCTC CCTGGATCTT GTGGCCCCAA TTCTTTCCAT GTTTTTTCTC
ATGTGTTACC TCTTTGTAAA CTTGGCATGT GCCTTGCAAA CATTACTTCG AACACCCAAC
TGGAGACCCC GATTCCGCTA CTACCATTGG GCCCTTTCTT TCATGGGAAT GAGTATCTGT
CTGGCTCTGA TGTTCATTTC TTCCTGGTAT TATGCCATTG TAGCCATGGT AATAGCTGGT
ATGATCTACA AGTACATTGA ATACCAAGGA GCTGAGAAAG AATGGGGTGA TGGTATCCGT
GGGCTGTCCC TCAGTGCAGC CCGGTTTGCT TTGCTTCGAT TGGAGGAAGG ACCTCCACAC
ACTAAAAACT GGAGGCCTCA GTTGCTTGTA TTACTGAAAC TAGATGAAGA CTTACATGTC
AAGCATCCTC GCCTCCTCAC CTTTGCCTCA CAGCTCAAAG CAGGAAAAGG TCTCACTATT
GTGGGCTCTG TCATCGTGGG GAACTTCCTA GAGAACTACG GTGAAGCTTT AGCTGCTGAG
CAGACCATAA AGCACCTAAT GGAGGCAGAG AAGGTAAAAG GATTCTGCCA GCTGGTGGTG
GCCGCCAAGC TGAGAGAGGG CATTTCCCAC CTCATCCAGT CATGTGGCCT TGGGGGCATG
AAGCACAACA CGGTGGTGAT GGGCTGGCCT AATGGCTGGC GTCAAAGCGA AGATGCCCGC
GCTTGGAAGA CTTTTATTGG CACAGTTCGA GTGACAACTG CTGCCCATCT TGCACTGCTG
GTGGCTAAAA ACATCTCCTT CTTTCCCAGC AATGTGGAGC AATTTTCTGA GGGCAACATT
GATGTGTGGT GGATTGTGCA TGATGGGGGG ATGCTTATGC TACTACCATT CCTACTGAAA
CAGCACAAGG TGTGGCGAAA GTGCAGCATA CGGATCTTCA CAGTAGCCCA ATTAGAAGAC
AACAGTATCC AAATGAAGAA GGACCTAGCC ACCTTCCTAT ATCACTTACG CATTGAGGCG
GAGGTAGAAG TGGTGGAGAT GCATGACAGT GATATATCAG CATATACTTA CGAGCGCACT
TTGATGATGG AACAAAGGTC CCAGATGCTC CGGCACATGC GGCTATCCAA AACAGAGCGA
GACAGAGAGG CACAATTGGT GAAAGACCGA AACTCAATGC TACGATTGAC CAGCATTGGC
TCTGATGAGG ACGAAGAGAC AGAAACCTAT CAGGAGAAGG TGCACATGAC TTGGACAAAA
GACAAGTACA TGGCATCCCG GGGACAAAAA GCGAAGTCAA TGGAAGGATT CCAGGACCTG
CTTAACATGC GTCCGGACCA GTCCAATGTG AGGCGGATGC ATACAGCAGT GAAACTCAAC
GAGGTTATAG TTAACAAGTC CCATGAAGCA AAGCTGGTTT TATTGAATAT GCCAGGGCCA
CCCCGAAACC CTGAGGGTGA TGAAAACTAC ATGGAGTTCC TAGAGGTGCT TACCGAGGGA
CTAGAGCGAG TCCTACTTGT CCGGGGTGGT GGCAGTGAAG TGATCACCAT TTATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005126.1
CDS143..3442
Misc Feature(1)89..91(+)
Misc Feature(2)314..3439(+)
Misc Feature(3)614..>1069(+)
Misc Feature(4)1532..2128(+)
Misc Feature(5)3041..3130(+)
Exon (1)1..260
Gene:SLC12A6
Gene Synonym:
Exon (2)261..305
Gene:SLC12A6
Gene Synonym:
Exon (3)306..400
Gene:SLC12A6
Gene Synonym:
Exon (4)401..532
Gene:SLC12A6
Gene Synonym:
Exon (5)533..679
Gene:SLC12A6
Gene Synonym:
Exon (6)680..734
Gene:SLC12A6
Gene Synonym:
Exon (7)735..865
Gene:SLC12A6
Gene Synonym:
Exon (8)866..1107
Gene:SLC12A6
Gene Synonym:
Exon (9)1108..1322
Gene:SLC12A6
Gene Synonym:
Exon (10)1323..1481
Gene:SLC12A6
Gene Synonym:
Exon (11)1482..1580
Gene:SLC12A6
Gene Synonym:
Exon (12)1581..1638
Gene:SLC12A6
Gene Synonym:
Exon (13)1639..1813
Gene:SLC12A6
Gene Synonym:
Exon (14)1814..1932
Gene:SLC12A6
Gene Synonym:
Exon (15)1933..2031
Gene:SLC12A6
Gene Synonym:
Exon (16)2032..2151
Gene:SLC12A6
Gene Synonym:
Exon (17)2152..2256
Gene:SLC12A6
Gene Synonym:
Exon (18)2257..2425
Gene:SLC12A6
Gene Synonym:
Exon (19)2426..2621
Gene:SLC12A6
Gene Synonym:
Exon (20)2622..2791
Gene:SLC12A6
Gene Synonym:
Exon (21)2792..2923
Gene:SLC12A6
Gene Synonym:
Exon (22)2924..3031
Gene:SLC12A6
Gene Synonym:
Exon (23)3032..3216
Gene:SLC12A6
Gene Synonym:
Exon (24)3217..3350
Gene:SLC12A6
Gene Synonym:
Exon (25)3351..7327
Gene:SLC12A6
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005135
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005135

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGCCACATT TTACTGTGAC TAAGGTAGAG GACCCAGAGG AGGGGGCAGC GGCTTCGATC 
TCTCAAGAGC CTAGTTTAGC AGACATAAAA GCCCGGATTC AGGATTCAGA TGAACCAGAC
CTGAGTCAGA ACTCCATCAC AGGGGAACAC AGCCAACTGT TAGACGACGG ACATAAGAAA
GCTCGAAATG CTTATCTCAA TAATTCCAAT TATGAAGAAG GAGATGAATA TTTTGATAAA
AATTTGGCAC TCTTTGAGGA AGAAATGGAC ACCAGACCGA AGGTGTCTTC CCTCCTCAAC
CGCATGGCCA ATTACACTAA TCTGACTCAA GGAGCAAAGG AACATGAAGA GGCAGAAAAC
ATCACTGAAG GGAAAAAGAA GCCCACCAAG ACCCCCCAAA TGGGTACCTT CATGGGTGTC
TACCTCCCAT GTCTACAAAA TATTTTTGGA GTGATCCTTT TTTTACGCCT TACATGGGTG
GTGGGCACAG CTGGAGTTCT TCAGGCTTTT GCAATTGTCC TTATCTGCTG CTGCTGTACA
ATGTTGACTG CTATCTCCAT GAGTGCCATT GCCACTAATG GAGTGGTGCC AGCTGGGGGC
TCATACTTTA TGATTTCCCG GGCACTGGGC CCAGAGTTTG GTGGGGCTGT TGGCCTCTGC
TTTTATCTTG GTACCACATT TGCAGCAGCC ATGTACATCC TTGGTGCCAT TGAAATCTTT
CTGGTCTATA TCGTCCCCCG AGCTGCCATC TTTCACAGTG ATGACGCACT CAAGGAATCA
GCAGCCATGC TAAATAACAT GCGTGTCTAC GGCACAGCTT TCTTGGTCCT TATGGTATTA
GTGGTATTTA TCGGCGTACG CTATGTGAAC AAGTTTGCCT CACTTTTCCT GGCCTGTGTC
ATTGTGTCCA TCTTGGCCAT CTATGCTGGA GCCATCAAGT CTTCTTTTGC TCCTCCACAC
TTCCCGGTCT GCATGCTGGG TAACCGCACC CTTTCATCAA GACACATTGA CGTTTGCTCT
AAGACCAAGG AAATTAACAA CATGACAGTC CCATCAAAGT TATGGGGATT CTTCTGTAAC
TCGAGTCAAT TTTTCAATGC CACCTGTGAT GAATACTTTG TTCACAATAA CGTCACTTCA
ATCCAGGGCA TTCCTGGATT GGCTAGTGGT ATAATTACAG AGAATCTTTG GAGTAATTAC
CTACCCAAGG GAGAGATCAT CGAAAAGCCT TCAGCCAAAT CTTCTGATGT CTTAGGCAGC
TTAAACCATG AATATGTTCT TGTTGACATC ACCACCTCCT TCACGCTTCT GGTGGGAATC
TTCTTTCCCT CTGTTACAGG TATCATGGCT GGATCAAACA GATCTGGAGA TCTGAAAGAT
GCTCAGAAGT CTATTCCGAT TGGTACTATC CTTGCCATCC TGACCACCTC CTTTGTTTAT
TTAAGCAATG TTGTCCTTTT TGGTGCATGT ATTGAAGGGG TTGTTCTCAG AGACAAGTTC
GGTGATGCTG TGAAAGGTAA TTTGGTGGTA GGCACCTTAT CTTGGCCATC CCCATGGGTG
ATTGTTATTG GCTCCTTCTT TTCAACATGT GGGGCTGGAC TTCAGAGCCT CACAGGTGCA
CCGAGGCTGC TACAAGCTAT TGCCAAGGAT AACATCATAC CGTTTCTGAG GGTTTTTGGC
CACAGCAAAG CCAATGGGGA ACCTACCTGG GCTTTACTTC TAACTGCTGC CATTGCAGAG
CTTGGAATAC TCATTGCCTC CCTGGATCTT GTGGCCCCAA TTCTTTCCAT GTTTTTTCTC
ATGTGTTACC TCTTTGTAAA CTTGGCATGT GCCTTGCAAA CATTACTTCG AACACCCAAC
TGGAGACCCC GATTCCGCTA CTACCATTGG GCCCTTTCTT TCATGGGAAT GAGTATCTGT
CTGGCTCTGA TGTTCATTTC TTCCTGGTAT TATGCCATTG TAGCCATGGT AATAGCTGGT
ATGATCTACA AGTACATTGA ATACCAAGGA GCTGAGAAAG AATGGGGTGA TGGTATCCGT
GGGCTGTCCC TCAGTGCAGC CCGGTTTGCT TTGCTTCGAT TGGAGGAAGG ACCTCCACAC
ACTAAAAACT GGAGGCCTCA GTTGCTTGTA TTACTGAAAC TAGATGAAGA CTTACATGTC
AAGCATCCTC GCCTCCTCAC CTTTGCCTCA CAGCTCAAAG CAGGAAAAGG TCTCACTATT
GTGGGCTCTG TCATCGTGGG GAACTTCCTA GAGAACTACG GTGAAGCTTT AGCTGCTGAG
CAGACCATAA AGCACCTAAT GGAGGCAGAG AAGGTAAAAG GATTCTGCCA GCTGGTGGTG
GCCGCCAAGC TGAGAGAGGG CATTTCCCAC CTCATCCAGT CATGTGGCCT TGGGGGCATG
AAGCACAACA CGGTGGTGAT GGGCTGGCCT AATGGCTGGC GTCAAAGCGA AGATGCCCGC
GCTTGGAAGA CTTTTATTGG CACAGTTCGA GTGACAACTG CTGCCCATCT TGCACTGCTG
GTGGCTAAAA ACATCTCCTT CTTTCCCAGC AATGTGGAGC AATTTTCTGA GGGCAACATT
GATGTGTGGT GGATTGTGCA TGATGGGGGG ATGCTTATGC TACTACCATT CCTACTGAAA
CAGCACAAGG TGTGGCGAAA GTGCAGCATA CGGATCTTCA CAGTAGCCCA ATTAGAAGAC
AACAGTATCC AAATGAAGAA GGACCTAGCC ACCTTCCTAT ATCACTTACG CATTGAGGCG
GAGGTAGAAG TGGTGGAGAT GCATGACAGT GATATATCAG CATATACTTA CGAGCGCACT
TTGATGATGG AACAAAGGTC CCAGATGCTC CGGCACATGC GGCTATCCAA AACAGAGCGA
GACAGAGAGG CACAATTGGT GAAAGACCGA AACTCAATGC TACGATTGAC CAGCATTGGC
TCTGATGAGG ACGAAGAGAC AGAAACCTAT CAGGAGAAGG TGCACATGAC TTGGACAAAA
GACAAGTACA TGGCATCCCG GGGACAAAAA GCGAAGTCAA TGGAAGGATT CCAGGACCTG
CTTAACATGC GTCCGGACCA GTCCAATGTG AGGCGGATGC ATACAGCAGT GAAACTCAAC
GAGGTTATAG TTAACAAGTC CCATGAAGCA AAGCTGGTTT TATTGAATAT GCCAGGGCCA
CCCCGAAACC CTGAGGGTGA TGAAAACTAC ATGGAGTTCC TAGAGGTGCT TACCGAGGGA
CTAGAGCGAG TCCTACTTGT CCGGGGTGGT GGCAGTGAAG TGATCACCAT TTATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16186
Clone ID Related Accession (Same CDS sequence) NM_001042494 , NM_001042495
Accession Version NM_001042494.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021822.21, AF531259.1 and AC079203.9. Transcript Variant: This variant (3) has an additional exon in its 5' UTR and uses an alternate in-frame splice site in the first protein coding exon, compared to variant 1. The encoded protein (isoform c) uses a downstream start codon, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF477977.1, AF531259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGAGTGAGA TGTCTGGGGC CACCACTTCG CTGGCAACTG TTGCACTGGA TCCACCCAGT 
GACCGGACTT CTCACCCCCA GGATGTCATC GAGGACCTGA GTCAGAACTC CATCACAGGG
GAACACAGCC AACTGTTAGA CGACGGACAT AAGAAAGCTC GAAATGCTTA TCTCAATAAT
TCCAATTATG AAGAAGGAGA TGAATATTTT GATAAAAATT TGGCACTCTT TGAGGAAGAA
ATGGACACCA GACCGAAGGT GTCTTCCCTC CTCAACCGCA TGGCCAATTA CACTAATCTG
ACTCAAGGAG CAAAGGAACA TGAAGAGGCA GAAAACATCA CTGAAGGGAA AAAGAAGCCC
ACCAAGACCC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
TTTGGAGTGA TCCTTTTTTT ACGCCTTACA TGGGTGGTGG GCACAGCTGG AGTTCTTCAG
GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACAATGT TGACTGCTAT CTCCATGAGT
GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGGGGCTCAT ACTTTATGAT TTCCCGGGCA
CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC CTCTGCTTTT ATCTTGGTAC CACATTTGCA
GCAGCCATGT ACATCCTTGG TGCCATTGAA ATCTTTCTGG TCTATATCGT CCCCCGAGCT
GCCATCTTTC ACAGTGATGA CGCACTCAAG GAATCAGCAG CCATGCTAAA TAACATGCGT
GTCTACGGCA CAGCTTTCTT GGTCCTTATG GTATTAGTGG TATTTATCGG CGTACGCTAT
GTGAACAAGT TTGCCTCACT TTTCCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAT
GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT CCACACTTCC CGGTCTGCAT GCTGGGTAAC
CGCACCCTTT CATCAAGACA CATTGACGTT TGCTCTAAGA CCAAGGAAAT TAACAACATG
ACAGTCCCAT CAAAGTTATG GGGATTCTTC TGTAACTCGA GTCAATTTTT CAATGCCACC
TGTGATGAAT ACTTTGTTCA CAATAACGTC ACTTCAATCC AGGGCATTCC TGGATTGGCT
AGTGGTATAA TTACAGAGAA TCTTTGGAGT AATTACCTAC CCAAGGGAGA GATCATCGAA
AAGCCTTCAG CCAAATCTTC TGATGTCTTA GGCAGCTTAA ACCATGAATA TGTTCTTGTT
GACATCACCA CCTCCTTCAC GCTTCTGGTG GGAATCTTCT TTCCCTCTGT TACAGGTATC
ATGGCTGGAT CAAACAGATC TGGAGATCTG AAAGATGCTC AGAAGTCTAT TCCGATTGGT
ACTATCCTTG CCATCCTGAC CACCTCCTTT GTTTATTTAA GCAATGTTGT CCTTTTTGGT
GCATGTATTG AAGGGGTTGT TCTCAGAGAC AAGTTCGGTG ATGCTGTGAA AGGTAATTTG
GTGGTAGGCA CCTTATCTTG GCCATCCCCA TGGGTGATTG TTATTGGCTC CTTCTTTTCA
ACATGTGGGG CTGGACTTCA GAGCCTCACA GGTGCACCGA GGCTGCTACA AGCTATTGCC
AAGGATAACA TCATACCGTT TCTGAGGGTT TTTGGCCACA GCAAAGCCAA TGGGGAACCT
ACCTGGGCTT TACTTCTAAC TGCTGCCATT GCAGAGCTTG GAATACTCAT TGCCTCCCTG
GATCTTGTGG CCCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTAAACTTG
GCATGTGCCT TGCAAACATT ACTTCGAACA CCCAACTGGA GACCCCGATT CCGCTACTAC
CATTGGGCCC TTTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
TGGTATTATG CCATTGTAGC CATGGTAATA GCTGGTATGA TCTACAAGTA CATTGAATAC
CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG
TTTGCTTTGC TTCGATTGGA GGAAGGACCT CCACACACTA AAAACTGGAG GCCTCAGTTG
CTTGTATTAC TGAAACTAGA TGAAGACTTA CATGTCAAGC ATCCTCGCCT CCTCACCTTT
GCCTCACAGC TCAAAGCAGG AAAAGGTCTC ACTATTGTGG GCTCTGTCAT CGTGGGGAAC
TTCCTAGAGA ACTACGGTGA AGCTTTAGCT GCTGAGCAGA CCATAAAGCA CCTAATGGAG
GCAGAGAAGG TAAAAGGATT CTGCCAGCTG GTGGTGGCCG CCAAGCTGAG AGAGGGCATT
TCCCACCTCA TCCAGTCATG TGGCCTTGGG GGCATGAAGC ACAACACGGT GGTGATGGGC
TGGCCTAATG GCTGGCGTCA AAGCGAAGAT GCCCGCGCTT GGAAGACTTT TATTGGCACA
GTTCGAGTGA CAACTGCTGC CCATCTTGCA CTGCTGGTGG CTAAAAACAT CTCCTTCTTT
CCCAGCAATG TGGAGCAATT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTGCATGAT
GGGGGGATGC TTATGCTACT ACCATTCCTA CTGAAACAGC ACAAGGTGTG GCGAAAGTGC
AGCATACGGA TCTTCACAGT AGCCCAATTA GAAGACAACA GTATCCAAAT GAAGAAGGAC
CTAGCCACCT TCCTATATCA CTTACGCATT GAGGCGGAGG TAGAAGTGGT GGAGATGCAT
GACAGTGATA TATCAGCATA TACTTACGAG CGCACTTTGA TGATGGAACA AAGGTCCCAG
ATGCTCCGGC ACATGCGGCT ATCCAAAACA GAGCGAGACA GAGAGGCACA ATTGGTGAAA
GACCGAAACT CAATGCTACG ATTGACCAGC ATTGGCTCTG ATGAGGACGA AGAGACAGAA
ACCTATCAGG AGAAGGTGCA CATGACTTGG ACAAAAGACA AGTACATGGC ATCCCGGGGA
CAAAAAGCGA AGTCAATGGA AGGATTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
AATGTGAGGC GGATGCATAC AGCAGTGAAA CTCAACGAGG TTATAGTTAA CAAGTCCCAT
GAAGCAAAGC TGGTTTTATT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGATGAA
AACTACATGG AGTTCCTAGA GGTGCTTACC GAGGGACTAG AGCGAGTCCT ACTTGTCCGG
GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001035959.1
CDS528..3803
Misc Feature(1)426..428(+)
Misc Feature(2)675..3800(+)
Misc Feature(3)975..>1430(+)
Misc Feature(4)1893..2489(+)
Misc Feature(5)3402..3491(+)
Exon (1)1..429
Gene:SLC12A6
Gene Synonym:
Exon (2)430..621
Gene:SLC12A6
Gene Synonym:
Exon (3)622..666
Gene:SLC12A6
Gene Synonym:
Exon (4)667..761
Gene:SLC12A6
Gene Synonym:
Exon (5)762..893
Gene:SLC12A6
Gene Synonym:
Exon (6)894..1040
Gene:SLC12A6
Gene Synonym:
Exon (7)1041..1095
Gene:SLC12A6
Gene Synonym:
Exon (8)1096..1226
Gene:SLC12A6
Gene Synonym:
Exon (9)1227..1468
Gene:SLC12A6
Gene Synonym:
Exon (10)1469..1683
Gene:SLC12A6
Gene Synonym:
Exon (11)1684..1842
Gene:SLC12A6
Gene Synonym:
Exon (12)1843..1941
Gene:SLC12A6
Gene Synonym:
Exon (13)1942..1999
Gene:SLC12A6
Gene Synonym:
Exon (14)2000..2174
Gene:SLC12A6
Gene Synonym:
Exon (15)2175..2293
Gene:SLC12A6
Gene Synonym:
Exon (16)2294..2392
Gene:SLC12A6
Gene Synonym:
Exon (17)2393..2512
Gene:SLC12A6
Gene Synonym:
Exon (18)2513..2617
Gene:SLC12A6
Gene Synonym:
Exon (19)2618..2786
Gene:SLC12A6
Gene Synonym:
Exon (20)2787..2982
Gene:SLC12A6
Gene Synonym:
Exon (21)2983..3152
Gene:SLC12A6
Gene Synonym:
Exon (22)3153..3284
Gene:SLC12A6
Gene Synonym:
Exon (23)3285..3392
Gene:SLC12A6
Gene Synonym:
Exon (24)3393..3577
Gene:SLC12A6
Gene Synonym:
Exon (25)3578..3711
Gene:SLC12A6
Gene Synonym:
Exon (26)3712..7688
Gene:SLC12A6
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001042494
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001042494

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGAGTGAGA TGTCTGGGGC CACCACTTCG CTGGCAACTG TTGCACTGGA TCCACCCAGT 
GACCGGACTT CTCACCCCCA GGATGTCATC GAGGACCTGA GTCAGAACTC CATCACAGGG
GAACACAGCC AACTGTTAGA CGACGGACAT AAGAAAGCTC GAAATGCTTA TCTCAATAAT
TCCAATTATG AAGAAGGAGA TGAATATTTT GATAAAAATT TGGCACTCTT TGAGGAAGAA
ATGGACACCA GACCGAAGGT GTCTTCCCTC CTCAACCGCA TGGCCAATTA CACTAATCTG
ACTCAAGGAG CAAAGGAACA TGAAGAGGCA GAAAACATCA CTGAAGGGAA AAAGAAGCCC
ACCAAGACCC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
TTTGGAGTGA TCCTTTTTTT ACGCCTTACA TGGGTGGTGG GCACAGCTGG AGTTCTTCAG
GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACAATGT TGACTGCTAT CTCCATGAGT
GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGGGGCTCAT ACTTTATGAT TTCCCGGGCA
CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC CTCTGCTTTT ATCTTGGTAC CACATTTGCA
GCAGCCATGT ACATCCTTGG TGCCATTGAA ATCTTTCTGG TCTATATCGT CCCCCGAGCT
GCCATCTTTC ACAGTGATGA CGCACTCAAG GAATCAGCAG CCATGCTAAA TAACATGCGT
GTCTACGGCA CAGCTTTCTT GGTCCTTATG GTATTAGTGG TATTTATCGG CGTACGCTAT
GTGAACAAGT TTGCCTCACT TTTCCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAT
GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT CCACACTTCC CGGTCTGCAT GCTGGGTAAC
CGCACCCTTT CATCAAGACA CATTGACGTT TGCTCTAAGA CCAAGGAAAT TAACAACATG
ACAGTCCCAT CAAAGTTATG GGGATTCTTC TGTAACTCGA GTCAATTTTT CAATGCCACC
TGTGATGAAT ACTTTGTTCA CAATAACGTC ACTTCAATCC AGGGCATTCC TGGATTGGCT
AGTGGTATAA TTACAGAGAA TCTTTGGAGT AATTACCTAC CCAAGGGAGA GATCATCGAA
AAGCCTTCAG CCAAATCTTC TGATGTCTTA GGCAGCTTAA ACCATGAATA TGTTCTTGTT
GACATCACCA CCTCCTTCAC GCTTCTGGTG GGAATCTTCT TTCCCTCTGT TACAGGTATC
ATGGCTGGAT CAAACAGATC TGGAGATCTG AAAGATGCTC AGAAGTCTAT TCCGATTGGT
ACTATCCTTG CCATCCTGAC CACCTCCTTT GTTTATTTAA GCAATGTTGT CCTTTTTGGT
GCATGTATTG AAGGGGTTGT TCTCAGAGAC AAGTTCGGTG ATGCTGTGAA AGGTAATTTG
GTGGTAGGCA CCTTATCTTG GCCATCCCCA TGGGTGATTG TTATTGGCTC CTTCTTTTCA
ACATGTGGGG CTGGACTTCA GAGCCTCACA GGTGCACCGA GGCTGCTACA AGCTATTGCC
AAGGATAACA TCATACCGTT TCTGAGGGTT TTTGGCCACA GCAAAGCCAA TGGGGAACCT
ACCTGGGCTT TACTTCTAAC TGCTGCCATT GCAGAGCTTG GAATACTCAT TGCCTCCCTG
GATCTTGTGG CCCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTAAACTTG
GCATGTGCCT TGCAAACATT ACTTCGAACA CCCAACTGGA GACCCCGATT CCGCTACTAC
CATTGGGCCC TTTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
TGGTATTATG CCATTGTAGC CATGGTAATA GCTGGTATGA TCTACAAGTA CATTGAATAC
CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG
TTTGCTTTGC TTCGATTGGA GGAAGGACCT CCACACACTA AAAACTGGAG GCCTCAGTTG
CTTGTATTAC TGAAACTAGA TGAAGACTTA CATGTCAAGC ATCCTCGCCT CCTCACCTTT
GCCTCACAGC TCAAAGCAGG AAAAGGTCTC ACTATTGTGG GCTCTGTCAT CGTGGGGAAC
TTCCTAGAGA ACTACGGTGA AGCTTTAGCT GCTGAGCAGA CCATAAAGCA CCTAATGGAG
GCAGAGAAGG TAAAAGGATT CTGCCAGCTG GTGGTGGCCG CCAAGCTGAG AGAGGGCATT
TCCCACCTCA TCCAGTCATG TGGCCTTGGG GGCATGAAGC ACAACACGGT GGTGATGGGC
TGGCCTAATG GCTGGCGTCA AAGCGAAGAT GCCCGCGCTT GGAAGACTTT TATTGGCACA
GTTCGAGTGA CAACTGCTGC CCATCTTGCA CTGCTGGTGG CTAAAAACAT CTCCTTCTTT
CCCAGCAATG TGGAGCAATT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTGCATGAT
GGGGGGATGC TTATGCTACT ACCATTCCTA CTGAAACAGC ACAAGGTGTG GCGAAAGTGC
AGCATACGGA TCTTCACAGT AGCCCAATTA GAAGACAACA GTATCCAAAT GAAGAAGGAC
CTAGCCACCT TCCTATATCA CTTACGCATT GAGGCGGAGG TAGAAGTGGT GGAGATGCAT
GACAGTGATA TATCAGCATA TACTTACGAG CGCACTTTGA TGATGGAACA AAGGTCCCAG
ATGCTCCGGC ACATGCGGCT ATCCAAAACA GAGCGAGACA GAGAGGCACA ATTGGTGAAA
GACCGAAACT CAATGCTACG ATTGACCAGC ATTGGCTCTG ATGAGGACGA AGAGACAGAA
ACCTATCAGG AGAAGGTGCA CATGACTTGG ACAAAAGACA AGTACATGGC ATCCCGGGGA
CAAAAAGCGA AGTCAATGGA AGGATTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
AATGTGAGGC GGATGCATAC AGCAGTGAAA CTCAACGAGG TTATAGTTAA CAAGTCCCAT
GAAGCAAAGC TGGTTTTATT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGATGAA
AACTACATGG AGTTCCTAGA GGTGCTTACC GAGGGACTAG AGCGAGTCCT ACTTGTCCGG
GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16186
Clone ID Related Accession (Same CDS sequence) NM_001042494 , NM_001042495
Accession Version NM_001042495.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021822.21, BC070107.1 and AC079203.9. Transcript Variant: This variant (4) has an additional exon in its 5' UTR and uses an alternate in-frame splice site in the first protein coding exon, compared to variant 1. The encoded protein (isoform c) uses a downstream start codon, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070107.1, AF531260.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGAGTGAGA TGTCTGGGGC CACCACTTCG CTGGCAACTG TTGCACTGGA TCCACCCAGT 
GACCGGACTT CTCACCCCCA GGATGTCATC GAGGACCTGA GTCAGAACTC CATCACAGGG
GAACACAGCC AACTGTTAGA CGACGGACAT AAGAAAGCTC GAAATGCTTA TCTCAATAAT
TCCAATTATG AAGAAGGAGA TGAATATTTT GATAAAAATT TGGCACTCTT TGAGGAAGAA
ATGGACACCA GACCGAAGGT GTCTTCCCTC CTCAACCGCA TGGCCAATTA CACTAATCTG
ACTCAAGGAG CAAAGGAACA TGAAGAGGCA GAAAACATCA CTGAAGGGAA AAAGAAGCCC
ACCAAGACCC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
TTTGGAGTGA TCCTTTTTTT ACGCCTTACA TGGGTGGTGG GCACAGCTGG AGTTCTTCAG
GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACAATGT TGACTGCTAT CTCCATGAGT
GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGGGGCTCAT ACTTTATGAT TTCCCGGGCA
CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC CTCTGCTTTT ATCTTGGTAC CACATTTGCA
GCAGCCATGT ACATCCTTGG TGCCATTGAA ATCTTTCTGG TCTATATCGT CCCCCGAGCT
GCCATCTTTC ACAGTGATGA CGCACTCAAG GAATCAGCAG CCATGCTAAA TAACATGCGT
GTCTACGGCA CAGCTTTCTT GGTCCTTATG GTATTAGTGG TATTTATCGG CGTACGCTAT
GTGAACAAGT TTGCCTCACT TTTCCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAT
GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT CCACACTTCC CGGTCTGCAT GCTGGGTAAC
CGCACCCTTT CATCAAGACA CATTGACGTT TGCTCTAAGA CCAAGGAAAT TAACAACATG
ACAGTCCCAT CAAAGTTATG GGGATTCTTC TGTAACTCGA GTCAATTTTT CAATGCCACC
TGTGATGAAT ACTTTGTTCA CAATAACGTC ACTTCAATCC AGGGCATTCC TGGATTGGCT
AGTGGTATAA TTACAGAGAA TCTTTGGAGT AATTACCTAC CCAAGGGAGA GATCATCGAA
AAGCCTTCAG CCAAATCTTC TGATGTCTTA GGCAGCTTAA ACCATGAATA TGTTCTTGTT
GACATCACCA CCTCCTTCAC GCTTCTGGTG GGAATCTTCT TTCCCTCTGT TACAGGTATC
ATGGCTGGAT CAAACAGATC TGGAGATCTG AAAGATGCTC AGAAGTCTAT TCCGATTGGT
ACTATCCTTG CCATCCTGAC CACCTCCTTT GTTTATTTAA GCAATGTTGT CCTTTTTGGT
GCATGTATTG AAGGGGTTGT TCTCAGAGAC AAGTTCGGTG ATGCTGTGAA AGGTAATTTG
GTGGTAGGCA CCTTATCTTG GCCATCCCCA TGGGTGATTG TTATTGGCTC CTTCTTTTCA
ACATGTGGGG CTGGACTTCA GAGCCTCACA GGTGCACCGA GGCTGCTACA AGCTATTGCC
AAGGATAACA TCATACCGTT TCTGAGGGTT TTTGGCCACA GCAAAGCCAA TGGGGAACCT
ACCTGGGCTT TACTTCTAAC TGCTGCCATT GCAGAGCTTG GAATACTCAT TGCCTCCCTG
GATCTTGTGG CCCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTAAACTTG
GCATGTGCCT TGCAAACATT ACTTCGAACA CCCAACTGGA GACCCCGATT CCGCTACTAC
CATTGGGCCC TTTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
TGGTATTATG CCATTGTAGC CATGGTAATA GCTGGTATGA TCTACAAGTA CATTGAATAC
CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG
TTTGCTTTGC TTCGATTGGA GGAAGGACCT CCACACACTA AAAACTGGAG GCCTCAGTTG
CTTGTATTAC TGAAACTAGA TGAAGACTTA CATGTCAAGC ATCCTCGCCT CCTCACCTTT
GCCTCACAGC TCAAAGCAGG AAAAGGTCTC ACTATTGTGG GCTCTGTCAT CGTGGGGAAC
TTCCTAGAGA ACTACGGTGA AGCTTTAGCT GCTGAGCAGA CCATAAAGCA CCTAATGGAG
GCAGAGAAGG TAAAAGGATT CTGCCAGCTG GTGGTGGCCG CCAAGCTGAG AGAGGGCATT
TCCCACCTCA TCCAGTCATG TGGCCTTGGG GGCATGAAGC ACAACACGGT GGTGATGGGC
TGGCCTAATG GCTGGCGTCA AAGCGAAGAT GCCCGCGCTT GGAAGACTTT TATTGGCACA
GTTCGAGTGA CAACTGCTGC CCATCTTGCA CTGCTGGTGG CTAAAAACAT CTCCTTCTTT
CCCAGCAATG TGGAGCAATT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTGCATGAT
GGGGGGATGC TTATGCTACT ACCATTCCTA CTGAAACAGC ACAAGGTGTG GCGAAAGTGC
AGCATACGGA TCTTCACAGT AGCCCAATTA GAAGACAACA GTATCCAAAT GAAGAAGGAC
CTAGCCACCT TCCTATATCA CTTACGCATT GAGGCGGAGG TAGAAGTGGT GGAGATGCAT
GACAGTGATA TATCAGCATA TACTTACGAG CGCACTTTGA TGATGGAACA AAGGTCCCAG
ATGCTCCGGC ACATGCGGCT ATCCAAAACA GAGCGAGACA GAGAGGCACA ATTGGTGAAA
GACCGAAACT CAATGCTACG ATTGACCAGC ATTGGCTCTG ATGAGGACGA AGAGACAGAA
ACCTATCAGG AGAAGGTGCA CATGACTTGG ACAAAAGACA AGTACATGGC ATCCCGGGGA
CAAAAAGCGA AGTCAATGGA AGGATTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
AATGTGAGGC GGATGCATAC AGCAGTGAAA CTCAACGAGG TTATAGTTAA CAAGTCCCAT
GAAGCAAAGC TGGTTTTATT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGATGAA
AACTACATGG AGTTCCTAGA GGTGCTTACC GAGGGACTAG AGCGAGTCCT ACTTGTCCGG
GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001035960.1
CDS203..3478
Misc Feature(1)350..3475(+)
Misc Feature(2)650..>1105(+)
Misc Feature(3)1568..2164(+)
Misc Feature(4)3077..3166(+)
Exon (1)1..104
Gene:SLC12A6
Gene Synonym:
Exon (2)105..296
Gene:SLC12A6
Gene Synonym:
Exon (3)297..341
Gene:SLC12A6
Gene Synonym:
Exon (4)342..436
Gene:SLC12A6
Gene Synonym:
Exon (5)437..568
Gene:SLC12A6
Gene Synonym:
Exon (6)569..715
Gene:SLC12A6
Gene Synonym:
Exon (7)716..770
Gene:SLC12A6
Gene Synonym:
Exon (8)771..901
Gene:SLC12A6
Gene Synonym:
Exon (9)902..1143
Gene:SLC12A6
Gene Synonym:
Exon (10)1144..1358
Gene:SLC12A6
Gene Synonym:
Exon (11)1359..1517
Gene:SLC12A6
Gene Synonym:
Exon (12)1518..1616
Gene:SLC12A6
Gene Synonym:
Exon (13)1617..1674
Gene:SLC12A6
Gene Synonym:
Exon (14)1675..1849
Gene:SLC12A6
Gene Synonym:
Exon (15)1850..1968
Gene:SLC12A6
Gene Synonym:
Exon (16)1969..2067
Gene:SLC12A6
Gene Synonym:
Exon (17)2068..2187
Gene:SLC12A6
Gene Synonym:
Exon (18)2188..2292
Gene:SLC12A6
Gene Synonym:
Exon (19)2293..2461
Gene:SLC12A6
Gene Synonym:
Exon (20)2462..2657
Gene:SLC12A6
Gene Synonym:
Exon (21)2658..2827
Gene:SLC12A6
Gene Synonym:
Exon (22)2828..2959
Gene:SLC12A6
Gene Synonym:
Exon (23)2960..3067
Gene:SLC12A6
Gene Synonym:
Exon (24)3068..3252
Gene:SLC12A6
Gene Synonym:
Exon (25)3253..3386
Gene:SLC12A6
Gene Synonym:
Exon (26)3387..7363
Gene:SLC12A6
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001042495
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001042495

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGAGTGAGA TGTCTGGGGC CACCACTTCG CTGGCAACTG TTGCACTGGA TCCACCCAGT 
GACCGGACTT CTCACCCCCA GGATGTCATC GAGGACCTGA GTCAGAACTC CATCACAGGG
GAACACAGCC AACTGTTAGA CGACGGACAT AAGAAAGCTC GAAATGCTTA TCTCAATAAT
TCCAATTATG AAGAAGGAGA TGAATATTTT GATAAAAATT TGGCACTCTT TGAGGAAGAA
ATGGACACCA GACCGAAGGT GTCTTCCCTC CTCAACCGCA TGGCCAATTA CACTAATCTG
ACTCAAGGAG CAAAGGAACA TGAAGAGGCA GAAAACATCA CTGAAGGGAA AAAGAAGCCC
ACCAAGACCC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
TTTGGAGTGA TCCTTTTTTT ACGCCTTACA TGGGTGGTGG GCACAGCTGG AGTTCTTCAG
GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACAATGT TGACTGCTAT CTCCATGAGT
GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGGGGCTCAT ACTTTATGAT TTCCCGGGCA
CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC CTCTGCTTTT ATCTTGGTAC CACATTTGCA
GCAGCCATGT ACATCCTTGG TGCCATTGAA ATCTTTCTGG TCTATATCGT CCCCCGAGCT
GCCATCTTTC ACAGTGATGA CGCACTCAAG GAATCAGCAG CCATGCTAAA TAACATGCGT
GTCTACGGCA CAGCTTTCTT GGTCCTTATG GTATTAGTGG TATTTATCGG CGTACGCTAT
GTGAACAAGT TTGCCTCACT TTTCCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAT
GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT CCACACTTCC CGGTCTGCAT GCTGGGTAAC
CGCACCCTTT CATCAAGACA CATTGACGTT TGCTCTAAGA CCAAGGAAAT TAACAACATG
ACAGTCCCAT CAAAGTTATG GGGATTCTTC TGTAACTCGA GTCAATTTTT CAATGCCACC
TGTGATGAAT ACTTTGTTCA CAATAACGTC ACTTCAATCC AGGGCATTCC TGGATTGGCT
AGTGGTATAA TTACAGAGAA TCTTTGGAGT AATTACCTAC CCAAGGGAGA GATCATCGAA
AAGCCTTCAG CCAAATCTTC TGATGTCTTA GGCAGCTTAA ACCATGAATA TGTTCTTGTT
GACATCACCA CCTCCTTCAC GCTTCTGGTG GGAATCTTCT TTCCCTCTGT TACAGGTATC
ATGGCTGGAT CAAACAGATC TGGAGATCTG AAAGATGCTC AGAAGTCTAT TCCGATTGGT
ACTATCCTTG CCATCCTGAC CACCTCCTTT GTTTATTTAA GCAATGTTGT CCTTTTTGGT
GCATGTATTG AAGGGGTTGT TCTCAGAGAC AAGTTCGGTG ATGCTGTGAA AGGTAATTTG
GTGGTAGGCA CCTTATCTTG GCCATCCCCA TGGGTGATTG TTATTGGCTC CTTCTTTTCA
ACATGTGGGG CTGGACTTCA GAGCCTCACA GGTGCACCGA GGCTGCTACA AGCTATTGCC
AAGGATAACA TCATACCGTT TCTGAGGGTT TTTGGCCACA GCAAAGCCAA TGGGGAACCT
ACCTGGGCTT TACTTCTAAC TGCTGCCATT GCAGAGCTTG GAATACTCAT TGCCTCCCTG
GATCTTGTGG CCCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTAAACTTG
GCATGTGCCT TGCAAACATT ACTTCGAACA CCCAACTGGA GACCCCGATT CCGCTACTAC
CATTGGGCCC TTTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
TGGTATTATG CCATTGTAGC CATGGTAATA GCTGGTATGA TCTACAAGTA CATTGAATAC
CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG
TTTGCTTTGC TTCGATTGGA GGAAGGACCT CCACACACTA AAAACTGGAG GCCTCAGTTG
CTTGTATTAC TGAAACTAGA TGAAGACTTA CATGTCAAGC ATCCTCGCCT CCTCACCTTT
GCCTCACAGC TCAAAGCAGG AAAAGGTCTC ACTATTGTGG GCTCTGTCAT CGTGGGGAAC
TTCCTAGAGA ACTACGGTGA AGCTTTAGCT GCTGAGCAGA CCATAAAGCA CCTAATGGAG
GCAGAGAAGG TAAAAGGATT CTGCCAGCTG GTGGTGGCCG CCAAGCTGAG AGAGGGCATT
TCCCACCTCA TCCAGTCATG TGGCCTTGGG GGCATGAAGC ACAACACGGT GGTGATGGGC
TGGCCTAATG GCTGGCGTCA AAGCGAAGAT GCCCGCGCTT GGAAGACTTT TATTGGCACA
GTTCGAGTGA CAACTGCTGC CCATCTTGCA CTGCTGGTGG CTAAAAACAT CTCCTTCTTT
CCCAGCAATG TGGAGCAATT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTGCATGAT
GGGGGGATGC TTATGCTACT ACCATTCCTA CTGAAACAGC ACAAGGTGTG GCGAAAGTGC
AGCATACGGA TCTTCACAGT AGCCCAATTA GAAGACAACA GTATCCAAAT GAAGAAGGAC
CTAGCCACCT TCCTATATCA CTTACGCATT GAGGCGGAGG TAGAAGTGGT GGAGATGCAT
GACAGTGATA TATCAGCATA TACTTACGAG CGCACTTTGA TGATGGAACA AAGGTCCCAG
ATGCTCCGGC ACATGCGGCT ATCCAAAACA GAGCGAGACA GAGAGGCACA ATTGGTGAAA
GACCGAAACT CAATGCTACG ATTGACCAGC ATTGGCTCTG ATGAGGACGA AGAGACAGAA
ACCTATCAGG AGAAGGTGCA CATGACTTGG ACAAAAGACA AGTACATGGC ATCCCGGGGA
CAAAAAGCGA AGTCAATGGA AGGATTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
AATGTGAGGC GGATGCATAC AGCAGTGAAA CTCAACGAGG TTATAGTTAA CAAGTCCCAT
GAAGCAAAGC TGGTTTTATT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGATGAA
AACTACATGG AGTTCCTAGA GGTGCTTACC GAGGGACTAG AGCGAGTCCT ACTTGTCCGG
GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16155
Clone ID Related Accession (Same CDS sequence) NM_001042496
Accession Version NM_001042496.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform d
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC021822.21, DQ138323.1 and AC079203.9. Transcript Variant: This variant (5) has an additional exon in its 5' UTR and uses an alternate in-frame splice site in the first protein coding exon, compared to variant 1. The encoded protein (isoform d) uses a downstream start codon, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ138323.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCTTCAG TTCGGTTCAT GGTGACACCG ACAAAGATCG ATGACATTCC AGGTTTGTCA 
GACACCAGTC CGGACCTCAG CTCTCGATCT AGTTCCCGAG TAAGATTTAG CTCCCGGGAA
AGCGTGCCTG AAACAAGCCG GAGTGAGCCT ATGAGTGAGA TGTCTGGGGC CACCACTTCG
CTGGCAACTG TTGCACTGGA TCCACCCAGT GACCGGACTT CTCACCCCCA GGATGTCATC
GAGGACCTGA GTCAGAACTC CATCACAGGG GAACACAGCC AACTGTTAGA CGACGGACAT
AAGAAAGCTC GAAATGCTTA TCTCAATAAT TCCAATTATG AAGAAGGAGA TGAATATTTT
GATAAAAATT TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCGAAGGT GTCTTCCCTC
CTCAACCGCA TGGCCAATTA CACTAATCTG ACTCAAGGAG CAAAGGAACA TGAAGAGGCA
GAAAACATCA CTGAAGGGAA AAAGAAGCCC ACCAAGACCC CCCAAATGGG TACCTTCATG
GGTGTCTACC TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTTTTTTT ACGCCTTACA
TGGGTGGTGG GCACAGCTGG AGTTCTTCAG GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC
TGTACAATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
GGGGGCTCAT ACTTTATGAT TTCCCGGGCA CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC
CTCTGCTTTT ATCTTGGTAC CACATTTGCA GCAGCCATGT ACATCCTTGG TGCCATTGAA
ATCTTTCTGG TCTATATCGT CCCCCGAGCT GCCATCTTTC ACAGTGATGA CGCACTCAAG
GAATCAGCAG CCATGCTAAA TAACATGCGT GTCTACGGCA CAGCTTTCTT GGTCCTTATG
GTATTAGTGG TATTTATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT TTTCCTGGCC
TGTGTCATTG TGTCCATCTT GGCCATCTAT GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT
CCACACTTCC CGGTCTGCAT GCTGGGTAAC CGCACCCTTT CATCAAGACA CATTGACGTT
TGCTCTAAGA CCAAGGAAAT TAACAACATG ACAGTCCCAT CAAAGTTATG GGGATTCTTC
TGTAACTCGA GTCAATTTTT CAATGCCACC TGTGATGAAT ACTTTGTTCA CAATAACGTC
ACTTCAATCC AGGGCATTCC TGGATTGGCT AGTGGTATAA TTACAGAGAA TCTTTGGAGT
AATTACCTAC CCAAGGGAGA GATCATCGAA AAGCCTTCAG CCAAATCTTC TGATGTCTTA
GGCAGCTTAA ACCATGAATA TGTTCTTGTT GACATCACCA CCTCCTTCAC GCTTCTGGTG
GGAATCTTCT TTCCCTCTGT TACAGGTATC ATGGCTGGAT CAAACAGATC TGGAGATCTG
AAAGATGCTC AGAAGTCTAT TCCGATTGGT ACTATCCTTG CCATCCTGAC CACCTCCTTT
GTTTATTTAA GCAATGTTGT CCTTTTTGGT GCATGTATTG AAGGGGTTGT TCTCAGAGAC
AAGTTCGGTG ATGCTGTGAA AGGTAATTTG GTGGTAGGCA CCTTATCTTG GCCATCCCCA
TGGGTGATTG TTATTGGCTC CTTCTTTTCA ACATGTGGGG CTGGACTTCA GAGCCTCACA
GGTGCACCGA GGCTGCTACA AGCTATTGCC AAGGATAACA TCATACCGTT TCTGAGGGTT
TTTGGCCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTAAC TGCTGCCATT
GCAGAGCTTG GAATACTCAT TGCCTCCCTG GATCTTGTGG CCCCAATTCT TTCCATGTTT
TTTCTCATGT GTTACCTCTT TGTAAACTTG GCATGTGCCT TGCAAACATT ACTTCGAACA
CCCAACTGGA GACCCCGATT CCGCTACTAC CATTGGGCCC TTTCTTTCAT GGGAATGAGT
ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATTGTAGC CATGGTAATA
GCTGGTATGA TCTACAAGTA CATTGAATAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG TTTGCTTTGC TTCGATTGGA GGAAGGACCT
CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTAC TGAAACTAGA TGAAGACTTA
CATGTCAAGC ATCCTCGCCT CCTCACCTTT GCCTCACAGC TCAAAGCAGG AAAAGGTCTC
ACTATTGTGG GCTCTGTCAT CGTGGGGAAC TTCCTAGAGA ACTACGGTGA AGCTTTAGCT
GCTGAGCAGA CCATAAAGCA CCTAATGGAG GCAGAGAAGG TAAAAGGATT CTGCCAGCTG
GTGGTGGCCG CCAAGCTGAG AGAGGGCATT TCCCACCTCA TCCAGTCATG TGGCCTTGGG
GGCATGAAGC ACAACACGGT GGTGATGGGC TGGCCTAATG GCTGGCGTCA AAGCGAAGAT
GCCCGCGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTTGCA
CTGCTGGTGG CTAAAAACAT CTCCTTCTTT CCCAGCAATG TGGAGCAATT TTCTGAGGGC
AACATTGATG TGTGGTGGAT TGTGCATGAT GGGGGGATGC TTATGCTACT ACCATTCCTA
CTGAAACAGC ACAAGGTGTG GCGAAAGTGC AGCATACGGA TCTTCACAGT AGCCCAATTA
GAAGACAACA GTATCCAAAT GAAGAAGGAC CTAGCCACCT TCCTATATCA CTTACGCATT
GAGGCGGAGG TAGAAGTGGT GGAGATGCAT GACAGTGATA TATCAGCATA TACTTACGAG
CGCACTTTGA TGATGGAACA AAGGTCCCAG ATGCTCCGGC ACATGCGGCT ATCCAAAACA
GAGCGAGACA GAGAGGCACA ATTGGTGAAA GACCGAAACT CAATGCTACG ATTGACCAGC
ATTGGCTCTG ATGAGGACGA AGAGACAGAA ACCTATCAGG AGAAGGTGCA CATGACTTGG
ACAAAAGACA AGTACATGGC ATCCCGGGGA CAAAAAGCGA AGTCAATGGA AGGATTCCAG
GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAA
CTCAACGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTATT GAATATGCCA
GGGCCACCCC GAAACCCTGA GGGTGATGAA AACTACATGG AGTTCCTAGA GGTGCTTACC
GAGGGACTAG AGCGAGTCCT ACTTGTCCGG GGTGGTGGCA GTGAAGTGAT CACCATTTAT
TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001035961.1
CDS444..3869
Misc Feature(1)429..431(+)
Misc Feature(2)741..3866(+)
Misc Feature(3)1041..>1496(+)
Misc Feature(4)1959..2555(+)
Misc Feature(5)3468..3557(+)
Exon (1)1..429
Gene:SLC12A6
Gene Synonym:
Exon (2)430..687
Gene:SLC12A6
Gene Synonym:
Exon (3)688..732
Gene:SLC12A6
Gene Synonym:
Exon (4)733..827
Gene:SLC12A6
Gene Synonym:
Exon (5)828..959
Gene:SLC12A6
Gene Synonym:
Exon (6)960..1106
Gene:SLC12A6
Gene Synonym:
Exon (7)1107..1161
Gene:SLC12A6
Gene Synonym:
Exon (8)1162..1292
Gene:SLC12A6
Gene Synonym:
Exon (9)1293..1534
Gene:SLC12A6
Gene Synonym:
Exon (10)1535..1749
Gene:SLC12A6
Gene Synonym:
Exon (11)1750..1908
Gene:SLC12A6
Gene Synonym:
Exon (12)1909..2007
Gene:SLC12A6
Gene Synonym:
Exon (13)2008..2065
Gene:SLC12A6
Gene Synonym:
Exon (14)2066..2240
Gene:SLC12A6
Gene Synonym:
Exon (15)2241..2359
Gene:SLC12A6
Gene Synonym:
Exon (16)2360..2458
Gene:SLC12A6
Gene Synonym:
Exon (17)2459..2578
Gene:SLC12A6
Gene Synonym:
Exon (18)2579..2683
Gene:SLC12A6
Gene Synonym:
Exon (19)2684..2852
Gene:SLC12A6
Gene Synonym:
Exon (20)2853..3048
Gene:SLC12A6
Gene Synonym:
Exon (21)3049..3218
Gene:SLC12A6
Gene Synonym:
Exon (22)3219..3350
Gene:SLC12A6
Gene Synonym:
Exon (23)3351..3458
Gene:SLC12A6
Gene Synonym:
Exon (24)3459..3643
Gene:SLC12A6
Gene Synonym:
Exon (25)3644..3777
Gene:SLC12A6
Gene Synonym:
Exon (26)3778..7754
Gene:SLC12A6
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001042496
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001042496

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGCTTCAG TTCGGTTCAT GGTGACACCG ACAAAGATCG ATGACATTCC AGGTTTGTCA 
GACACCAGTC CGGACCTCAG CTCTCGATCT AGTTCCCGAG TAAGATTTAG CTCCCGGGAA
AGCGTGCCTG AAACAAGCCG GAGTGAGCCT ATGAGTGAGA TGTCTGGGGC CACCACTTCG
CTGGCAACTG TTGCACTGGA TCCACCCAGT GACCGGACTT CTCACCCCCA GGATGTCATC
GAGGACCTGA GTCAGAACTC CATCACAGGG GAACACAGCC AACTGTTAGA CGACGGACAT
AAGAAAGCTC GAAATGCTTA TCTCAATAAT TCCAATTATG AAGAAGGAGA TGAATATTTT
GATAAAAATT TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCGAAGGT GTCTTCCCTC
CTCAACCGCA TGGCCAATTA CACTAATCTG ACTCAAGGAG CAAAGGAACA TGAAGAGGCA
GAAAACATCA CTGAAGGGAA AAAGAAGCCC ACCAAGACCC CCCAAATGGG TACCTTCATG
GGTGTCTACC TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTTTTTTT ACGCCTTACA
TGGGTGGTGG GCACAGCTGG AGTTCTTCAG GCTTTTGCAA TTGTCCTTAT CTGCTGCTGC
TGTACAATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
GGGGGCTCAT ACTTTATGAT TTCCCGGGCA CTGGGCCCAG AGTTTGGTGG GGCTGTTGGC
CTCTGCTTTT ATCTTGGTAC CACATTTGCA GCAGCCATGT ACATCCTTGG TGCCATTGAA
ATCTTTCTGG TCTATATCGT CCCCCGAGCT GCCATCTTTC ACAGTGATGA CGCACTCAAG
GAATCAGCAG CCATGCTAAA TAACATGCGT GTCTACGGCA CAGCTTTCTT GGTCCTTATG
GTATTAGTGG TATTTATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT TTTCCTGGCC
TGTGTCATTG TGTCCATCTT GGCCATCTAT GCTGGAGCCA TCAAGTCTTC TTTTGCTCCT
CCACACTTCC CGGTCTGCAT GCTGGGTAAC CGCACCCTTT CATCAAGACA CATTGACGTT
TGCTCTAAGA CCAAGGAAAT TAACAACATG ACAGTCCCAT CAAAGTTATG GGGATTCTTC
TGTAACTCGA GTCAATTTTT CAATGCCACC TGTGATGAAT ACTTTGTTCA CAATAACGTC
ACTTCAATCC AGGGCATTCC TGGATTGGCT AGTGGTATAA TTACAGAGAA TCTTTGGAGT
AATTACCTAC CCAAGGGAGA GATCATCGAA AAGCCTTCAG CCAAATCTTC TGATGTCTTA
GGCAGCTTAA ACCATGAATA TGTTCTTGTT GACATCACCA CCTCCTTCAC GCTTCTGGTG
GGAATCTTCT TTCCCTCTGT TACAGGTATC ATGGCTGGAT CAAACAGATC TGGAGATCTG
AAAGATGCTC AGAAGTCTAT TCCGATTGGT ACTATCCTTG CCATCCTGAC CACCTCCTTT
GTTTATTTAA GCAATGTTGT CCTTTTTGGT GCATGTATTG AAGGGGTTGT TCTCAGAGAC
AAGTTCGGTG ATGCTGTGAA AGGTAATTTG GTGGTAGGCA CCTTATCTTG GCCATCCCCA
TGGGTGATTG TTATTGGCTC CTTCTTTTCA ACATGTGGGG CTGGACTTCA GAGCCTCACA
GGTGCACCGA GGCTGCTACA AGCTATTGCC AAGGATAACA TCATACCGTT TCTGAGGGTT
TTTGGCCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTAAC TGCTGCCATT
GCAGAGCTTG GAATACTCAT TGCCTCCCTG GATCTTGTGG CCCCAATTCT TTCCATGTTT
TTTCTCATGT GTTACCTCTT TGTAAACTTG GCATGTGCCT TGCAAACATT ACTTCGAACA
CCCAACTGGA GACCCCGATT CCGCTACTAC CATTGGGCCC TTTCTTTCAT GGGAATGAGT
ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATTGTAGC CATGGTAATA
GCTGGTATGA TCTACAAGTA CATTGAATAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
ATCCGTGGGC TGTCCCTCAG TGCAGCCCGG TTTGCTTTGC TTCGATTGGA GGAAGGACCT
CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTAC TGAAACTAGA TGAAGACTTA
CATGTCAAGC ATCCTCGCCT CCTCACCTTT GCCTCACAGC TCAAAGCAGG AAAAGGTCTC
ACTATTGTGG GCTCTGTCAT CGTGGGGAAC TTCCTAGAGA ACTACGGTGA AGCTTTAGCT
GCTGAGCAGA CCATAAAGCA CCTAATGGAG GCAGAGAAGG TAAAAGGATT CTGCCAGCTG
GTGGTGGCCG CCAAGCTGAG AGAGGGCATT TCCCACCTCA TCCAGTCATG TGGCCTTGGG
GGCATGAAGC ACAACACGGT GGTGATGGGC TGGCCTAATG GCTGGCGTCA AAGCGAAGAT
GCCCGCGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTTGCA
CTGCTGGTGG CTAAAAACAT CTCCTTCTTT CCCAGCAATG TGGAGCAATT TTCTGAGGGC
AACATTGATG TGTGGTGGAT TGTGCATGAT GGGGGGATGC TTATGCTACT ACCATTCCTA
CTGAAACAGC ACAAGGTGTG GCGAAAGTGC AGCATACGGA TCTTCACAGT AGCCCAATTA
GAAGACAACA GTATCCAAAT GAAGAAGGAC CTAGCCACCT TCCTATATCA CTTACGCATT
GAGGCGGAGG TAGAAGTGGT GGAGATGCAT GACAGTGATA TATCAGCATA TACTTACGAG
CGCACTTTGA TGATGGAACA AAGGTCCCAG ATGCTCCGGC ACATGCGGCT ATCCAAAACA
GAGCGAGACA GAGAGGCACA ATTGGTGAAA GACCGAAACT CAATGCTACG ATTGACCAGC
ATTGGCTCTG ATGAGGACGA AGAGACAGAA ACCTATCAGG AGAAGGTGCA CATGACTTGG
ACAAAAGACA AGTACATGGC ATCCCGGGGA CAAAAAGCGA AGTCAATGGA AGGATTCCAG
GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAA
CTCAACGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTATT GAATATGCCA
GGGCCACCCC GAAACCCTGA GGGTGATGAA AACTACATGG AGTTCCTAGA GGTGCTTACC
GAGGGACTAG AGCGAGTCCT ACTTGTCCGG GGTGGTGGCA GTGAAGTGAT CACCATTTAT
TCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16248
Clone ID Related Accession (Same CDS sequence) NM_001042497
Accession Version NM_001042497.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product solute carrier family 12 member 6 isoform e
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF531258.1 and AC079203.9. Transcript Variant: This variant (6) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded protein (isoform e) is shorter, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF531258.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACGACGGAC ATAAGAAAGC TCGAAATGCT
TATCTCAATA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAAAA TTTGGCACTC
TTTGAGGAAG AAATGGACAC CAGACCGAAG GTGTCTTCCC TCCTCAACCG CATGGCCAAT
TACACTAATC TGACTCAAGG AGCAAAGGAA CATGAAGAGG CAGAAAACAT CACTGAAGGG
AAAAAGAAGC CCACCAAGAC CCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT
CTACAAAATA TTTTTGGAGT GATCCTTTTT TTACGCCTTA CATGGGTGGT GGGCACAGCT
GGAGTTCTTC AGGCTTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACAAT GTTGACTGCT
ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGGGGCTC ATACTTTATG
ATTTCCCGGG CACTGGGCCC AGAGTTTGGT GGGGCTGTTG GCCTCTGCTT TTATCTTGGT
ACCACATTTG CAGCAGCCAT GTACATCCTT GGTGCCATTG AAATCTTTCT GGTCTATATC
GTCCCCCGAG CTGCCATCTT TCACAGTGAT GACGCACTCA AGGAATCAGC AGCCATGCTA
AATAACATGC GTGTCTACGG CACAGCTTTC TTGGTCCTTA TGGTATTAGT GGTATTTATC
GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTTTTCCTGG CCTGTGTCAT TGTGTCCATC
TTGGCCATCT ATGCTGGAGC CATCAAGTCT TCTTTTGCTC CTCCACACTT CCCGGTCTGC
ATGCTGGGTA ACCGCACCCT TTCATCAAGA CACATTGACG TTTGCTCTAA GACCAAGGAA
ATTAACAACA TGACAGTCCC ATCAAAGTTA TGGGGATTCT TCTGTAACTC GAGTCAATTT
TTCAATGCCA CCTGTGATGA ATACTTTGTT CACAATAACG TCACTTCAAT CCAGGGCATT
CCTGGATTGG CTAGTGGTAT AATTACAGAG AATCTTTGGA GTAATTACCT ACCCAAGGGA
GAGATCATCG AAAAGCCTTC AGCCAAATCT TCTGATGTCT TAGGCAGCTT AAACCATGAA
TATGTTCTTG TTGACATCAC CACCTCCTTC ACGCTTCTGG TGGGAATCTT CTTTCCCTCT
GTTACAGGTA TCATGGCTGG ATCAAACAGA TCTGGAGATC TGAAAGATGC TCAGAAGTCT
ATTCCGATTG GTACTATCCT TGCCATCCTG ACCACCTCCT TTGTTTATTT AAGCAATGTT
GTCCTTTTTG GTGCATGTAT TGAAGGGGTT GTTCTCAGAG ACAAGTTCGG TGATGCTGTG
AAAGGTAATT TGGTGGTAGG CACCTTATCT TGGCCATCCC CATGGGTGAT TGTTATTGGC
TCCTTCTTTT CAACATGTGG GGCTGGACTT CAGAGCCTCA CAGGTGCACC GAGGCTGCTA
CAAGCTATTG CCAAGGATAA CATCATACCG TTTCTGAGGG TTTTTGGCCA CAGCAAAGCC
AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA TTGCAGAGCT TGGAATACTC
ATTGCCTCCC TGGATCTTGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC
TTTGTAAACT TGGCATGTGC CTTGCAAACA TTACTTCGAA CACCCAACTG GAGACCCCGA
TTCCGCTACT ACCATTGGGC CCTTTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG
TTCATTTCTT CCTGGTATTA TGCCATTGTA GCCATGGTAA TAGCTGGTAT GATCTACAAG
TACATTGAAT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGTGG GCTGTCCCTC
AGTGCAGCCC GGTTTGCTTT GCTTCGATTG GAGGAAGGAC CTCCACACAC TAAAAACTGG
AGGCCTCAGT TGCTTGTATT ACTGAAACTA GATGAAGACT TACATGTCAA GCATCCTCGC
CTCCTCACCT TTGCCTCACA GCTCAAAGCA GGAAAAGGTC TCACTATTGT GGGCTCTGTC
ATCGTGGGGA ACTTCCTAGA GAACTACGGT GAAGCTTTAG CTGCTGAGCA GACCATAAAG
CACCTAATGG AGGCAGAGAA GGTAAAAGGA TTCTGCCAGC TGGTGGTGGC CGCCAAGCTG
AGAGAGGGCA TTTCCCACCT CATCCAGTCA TGTGGCCTTG GGGGCATGAA GCACAACACG
GTGGTGATGG GCTGGCCTAA TGGCTGGCGT CAAAGCGAAG ATGCCCGCGC TTGGAAGACT
TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTTG CACTGCTGGT GGCTAAAAAC
ATCTCCTTCT TTCCCAGCAA TGTGGAGCAA TTTTCTGAGG GCAACATTGA TGTGTGGTGG
ATTGTGCATG ATGGGGGGAT GCTTATGCTA CTACCATTCC TACTGAAACA GCACAAGGTG
TGGCGAAAGT GCAGCATACG GATCTTCACA GTAGCCCAAT TAGAAGACAA CAGTATCCAA
ATGAAGAAGG ACCTAGCCAC CTTCCTATAT CACTTACGCA TTGAGGCGGA GGTAGAAGTG
GTGGAGATGC ATGACAGTGA TATATCAGCA TATACTTACG AGCGCACTTT GATGATGGAA
CAAAGGTCCC AGATGCTCCG GCACATGCGG CTATCCAAAA CAGAGCGAGA CAGAGAGGCA
CAATTGGTGA AAGACCGAAA CTCAATGCTA CGATTGACCA GCATTGGCTC TGATGAGGAC
GAAGAGACAG AAACCTATCA GGAGAAGGTG CACATGACTT GGACAAAAGA CAAGTACATG
GCATCCCGGG GACAAAAAGC GAAGTCAATG GAAGGATTCC AGGACCTGCT TAACATGCGT
CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AACTCAACGA GGTTATAGTT
AACAAGTCCC ATGAAGCAAA GCTGGTTTTA TTGAATATGC CAGGGCCACC CCGAAACCCT
GAGGGTGATG AAAACTACAT GGAGTTCCTA GAGGTGCTTA CCGAGGGACT AGAGCGAGTC
CTACTTGTCC GGGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001035962.1
CDS165..3572
Misc Feature(1)54..56(+)
Misc Feature(2)258..260(+)
Misc Feature(3)444..3569(+)
Misc Feature(4)675..743(+)
Misc Feature(5)744..>1199(+)
Misc Feature(6)789..854(+)
Misc Feature(7)933..1004(+)
Misc Feature(8)1074..1148(+)
Misc Feature(9)1158..1232(+)
Misc Feature(10)1557..1622(+)
Misc Feature(11)1662..2258(+)
Misc Feature(12)1665..1748(+)
Misc Feature(13)1791..1862(+)
Misc Feature(14)1980..2051(+)
Misc Feature(15)2061..2129(+)
Misc Feature(16)2148..2204(+)
Misc Feature(17)2208..2276(+)
Misc Feature(18)3171..3260(+)
Misc Feature(19)3213..3215(+)
Exon (1)1..435
Gene:SLC12A6
Gene Synonym:
Exon (2)436..530
Gene:SLC12A6
Gene Synonym:
Exon (3)531..662
Gene:SLC12A6
Gene Synonym:
Exon (4)663..809
Gene:SLC12A6
Gene Synonym:
Exon (5)810..864
Gene:SLC12A6
Gene Synonym:
Exon (6)865..995
Gene:SLC12A6
Gene Synonym:
Exon (7)996..1237
Gene:SLC12A6
Gene Synonym:
Exon (8)1238..1452
Gene:SLC12A6
Gene Synonym:
Exon (9)1453..1611
Gene:SLC12A6
Gene Synonym:
Exon (10)1612..1710
Gene:SLC12A6
Gene Synonym:
Exon (11)1711..1768
Gene:SLC12A6
Gene Synonym:
Exon (12)1769..1943
Gene:SLC12A6
Gene Synonym:
Exon (13)1944..2062
Gene:SLC12A6
Gene Synonym:
Exon (14)2063..2161
Gene:SLC12A6
Gene Synonym:
Exon (15)2162..2281
Gene:SLC12A6
Gene Synonym:
Exon (16)2282..2386
Gene:SLC12A6
Gene Synonym:
Exon (17)2387..2555
Gene:SLC12A6
Gene Synonym:
Exon (18)2556..2751
Gene:SLC12A6
Gene Synonym:
Exon (19)2752..2921
Gene:SLC12A6
Gene Synonym:
Exon (20)2922..3053
Gene:SLC12A6
Gene Synonym:
Exon (21)3054..3161
Gene:SLC12A6
Gene Synonym:
Exon (22)3162..3346
Gene:SLC12A6
Gene Synonym:
Exon (23)3347..3480
Gene:SLC12A6
Gene Synonym:
Exon (24)3481..7457
Gene:SLC12A6
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001042497
Organism Homo sapiens (human)
Definition Homo sapiens solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001042497

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCGACA 
AAGATCGATG ACATTCCAGG TTTGTCAGAC ACCAGTCCGG ACCTCAGCTC TCGATCTAGT
TCCCGAGTAA GATTTAGCTC CCGGGAAAGC GTGCCTGAAA CAAGCCGGAG TGAGCCTATG
AGTGAGATGT CTGGGGCCAC CACTTCGCTG GCAACTGTTG CACTGGATCC ACCCAGTGAC
CGGACTTCTC ACCCCCAGGA TGTCATCGAG GACGACGGAC ATAAGAAAGC TCGAAATGCT
TATCTCAATA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAAAA TTTGGCACTC
TTTGAGGAAG AAATGGACAC CAGACCGAAG GTGTCTTCCC TCCTCAACCG CATGGCCAAT
TACACTAATC TGACTCAAGG AGCAAAGGAA CATGAAGAGG CAGAAAACAT CACTGAAGGG
AAAAAGAAGC CCACCAAGAC CCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT
CTACAAAATA TTTTTGGAGT GATCCTTTTT TTACGCCTTA CATGGGTGGT GGGCACAGCT
GGAGTTCTTC AGGCTTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACAAT GTTGACTGCT
ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGGGGCTC ATACTTTATG
ATTTCCCGGG CACTGGGCCC AGAGTTTGGT GGGGCTGTTG GCCTCTGCTT TTATCTTGGT
ACCACATTTG CAGCAGCCAT GTACATCCTT GGTGCCATTG AAATCTTTCT GGTCTATATC
GTCCCCCGAG CTGCCATCTT TCACAGTGAT GACGCACTCA AGGAATCAGC AGCCATGCTA
AATAACATGC GTGTCTACGG CACAGCTTTC TTGGTCCTTA TGGTATTAGT GGTATTTATC
GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTTTTCCTGG CCTGTGTCAT TGTGTCCATC
TTGGCCATCT ATGCTGGAGC CATCAAGTCT TCTTTTGCTC CTCCACACTT CCCGGTCTGC
ATGCTGGGTA ACCGCACCCT TTCATCAAGA CACATTGACG TTTGCTCTAA GACCAAGGAA
ATTAACAACA TGACAGTCCC ATCAAAGTTA TGGGGATTCT TCTGTAACTC GAGTCAATTT
TTCAATGCCA CCTGTGATGA ATACTTTGTT CACAATAACG TCACTTCAAT CCAGGGCATT
CCTGGATTGG CTAGTGGTAT AATTACAGAG AATCTTTGGA GTAATTACCT ACCCAAGGGA
GAGATCATCG AAAAGCCTTC AGCCAAATCT TCTGATGTCT TAGGCAGCTT AAACCATGAA
TATGTTCTTG TTGACATCAC CACCTCCTTC ACGCTTCTGG TGGGAATCTT CTTTCCCTCT
GTTACAGGTA TCATGGCTGG ATCAAACAGA TCTGGAGATC TGAAAGATGC TCAGAAGTCT
ATTCCGATTG GTACTATCCT TGCCATCCTG ACCACCTCCT TTGTTTATTT AAGCAATGTT
GTCCTTTTTG GTGCATGTAT TGAAGGGGTT GTTCTCAGAG ACAAGTTCGG TGATGCTGTG
AAAGGTAATT TGGTGGTAGG CACCTTATCT TGGCCATCCC CATGGGTGAT TGTTATTGGC
TCCTTCTTTT CAACATGTGG GGCTGGACTT CAGAGCCTCA CAGGTGCACC GAGGCTGCTA
CAAGCTATTG CCAAGGATAA CATCATACCG TTTCTGAGGG TTTTTGGCCA CAGCAAAGCC
AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA TTGCAGAGCT TGGAATACTC
ATTGCCTCCC TGGATCTTGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC
TTTGTAAACT TGGCATGTGC CTTGCAAACA TTACTTCGAA CACCCAACTG GAGACCCCGA
TTCCGCTACT ACCATTGGGC CCTTTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG
TTCATTTCTT CCTGGTATTA TGCCATTGTA GCCATGGTAA TAGCTGGTAT GATCTACAAG
TACATTGAAT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGTGG GCTGTCCCTC
AGTGCAGCCC GGTTTGCTTT GCTTCGATTG GAGGAAGGAC CTCCACACAC TAAAAACTGG
AGGCCTCAGT TGCTTGTATT ACTGAAACTA GATGAAGACT TACATGTCAA GCATCCTCGC
CTCCTCACCT TTGCCTCACA GCTCAAAGCA GGAAAAGGTC TCACTATTGT GGGCTCTGTC
ATCGTGGGGA ACTTCCTAGA GAACTACGGT GAAGCTTTAG CTGCTGAGCA GACCATAAAG
CACCTAATGG AGGCAGAGAA GGTAAAAGGA TTCTGCCAGC TGGTGGTGGC CGCCAAGCTG
AGAGAGGGCA TTTCCCACCT CATCCAGTCA TGTGGCCTTG GGGGCATGAA GCACAACACG
GTGGTGATGG GCTGGCCTAA TGGCTGGCGT CAAAGCGAAG ATGCCCGCGC TTGGAAGACT
TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTTG CACTGCTGGT GGCTAAAAAC
ATCTCCTTCT TTCCCAGCAA TGTGGAGCAA TTTTCTGAGG GCAACATTGA TGTGTGGTGG
ATTGTGCATG ATGGGGGGAT GCTTATGCTA CTACCATTCC TACTGAAACA GCACAAGGTG
TGGCGAAAGT GCAGCATACG GATCTTCACA GTAGCCCAAT TAGAAGACAA CAGTATCCAA
ATGAAGAAGG ACCTAGCCAC CTTCCTATAT CACTTACGCA TTGAGGCGGA GGTAGAAGTG
GTGGAGATGC ATGACAGTGA TATATCAGCA TATACTTACG AGCGCACTTT GATGATGGAA
CAAAGGTCCC AGATGCTCCG GCACATGCGG CTATCCAAAA CAGAGCGAGA CAGAGAGGCA
CAATTGGTGA AAGACCGAAA CTCAATGCTA CGATTGACCA GCATTGGCTC TGATGAGGAC
GAAGAGACAG AAACCTATCA GGAGAAGGTG CACATGACTT GGACAAAAGA CAAGTACATG
GCATCCCGGG GACAAAAAGC GAAGTCAATG GAAGGATTCC AGGACCTGCT TAACATGCGT
CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AACTCAACGA GGTTATAGTT
AACAAGTCCC ATGAAGCAAA GCTGGTTTTA TTGAATATGC CAGGGCCACC CCGAAACCCT
GAGGGTGATG AAAACTACAT GGAGTTCCTA GAGGTGCTTA CCGAGGGACT AGAGCGAGTC
CTACTTGTCC GGGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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