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Btk Bruton agammaglobulinemia tyrosine kinase [Mus musculus (house mouse)]

Gene Symbol Btk
Entrez Gene ID 12229
Full Name Bruton agammaglobulinemia tyrosine kinase
Synonyms AI528679, xid
General protein information
Preferred Names
tyrosine-protein kinase BTK
Names
tyrosine-protein kinase BTK
ATK
BPK
kinase EMB
bruton tyrosine kinase
B-cell progenitor kinase
Bruton's tyrosine kinase
X-linked immune deficiency
agammaglobulinemia tyrosine kinase
agammaglobulinaemia tyrosine kinase
Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB)
NP_038510.2
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

X

X E3|X 56.18 cM

Summary lac of sum
Disorder MIM:

Disorder Html:





Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Btk gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Btk gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu18686 XM_006528483 PREDICTED: Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $439
OMu18686 XM_006528484 PREDICTED: Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $439
OMu18686 XM_006528485 PREDICTED: Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $439
OMu18686 NM_013482 Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OMu18686
Accession Version XM_006528483.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tyrosine-protein kinase BTK isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039716.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:569011231. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006528546.1
CDS291..2270
Misc Feature(1)306..788(+)
Misc Feature(2)312..638(+)
Misc Feature(3)324..449(+)
Misc Feature(4)717..785(+)
Misc Feature(5)939..1103(+)
Misc Feature(6)957..1094(+)
Misc Feature(7)1110..1424(+)
Misc Feature(8)1152..1211(+)
Misc Feature(9)1371..1400(+)
Misc Feature(10)1479..2246(+)
Misc Feature(11)1494..2243(+)
Misc Feature(12)1512..2099(+)
Misc Feature(13)1512..1916(+)
Misc Feature(14)1851..2099(+)
Misc Feature(15)1902..1976(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006528483
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006528483

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu18686
Accession Version XM_006528484.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tyrosine-protein kinase BTK isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039716.8) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:569011233. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006528547.1
CDS228..2207
Misc Feature(1)243..725(+)
Misc Feature(2)249..575(+)
Misc Feature(3)261..386(+)
Misc Feature(4)654..722(+)
Misc Feature(5)876..1040(+)
Misc Feature(6)894..1031(+)
Misc Feature(7)1047..1361(+)
Misc Feature(8)1089..1148(+)
Misc Feature(9)1308..1337(+)
Misc Feature(10)1416..2183(+)
Misc Feature(11)1431..2180(+)
Misc Feature(12)1449..2036(+)
Misc Feature(13)1449..1853(+)
Misc Feature(14)1788..2036(+)
Misc Feature(15)1839..1913(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006528484
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006528484

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu18686
Accession Version XM_006528485.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tyrosine-protein kinase BTK isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039716.8) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:569011235. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006528548.1
CDS202..2181
Misc Feature(1)217..699(+)
Misc Feature(2)223..549(+)
Misc Feature(3)235..360(+)
Misc Feature(4)628..696(+)
Misc Feature(5)850..1014(+)
Misc Feature(6)868..1005(+)
Misc Feature(7)1021..1335(+)
Misc Feature(8)1063..1122(+)
Misc Feature(9)1282..1311(+)
Misc Feature(10)1390..2157(+)
Misc Feature(11)1405..2154(+)
Misc Feature(12)1423..2010(+)
Misc Feature(13)1423..1827(+)
Misc Feature(14)1762..2010(+)
Misc Feature(15)1813..1887(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006528485
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006528485

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu18686
Accession Version NM_013482.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product tyrosine-protein kinase BTK
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ044967.1, L29788.1, DT921900.1 and BY596531.1. On Nov 24, 2007 this sequence version replaced gi:7709993. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L08967.1, L29788.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_038510.2
CDS161..2140
Misc Feature(1)116..118(+)
Misc Feature(2)176..658(+)
Misc Feature(3)182..508(+)
Misc Feature(4)194..319(+)
Misc Feature(5)194..232(+)
Misc Feature(6)278..280(+)
Misc Feature(7)587..655(+)
Misc Feature(8)809..973(+)
Misc Feature(9)827..964(+)
Misc Feature(10)827..829(+)
Misc Feature(11)980..1294(+)
Misc Feature(12)1022..1081(+)
Misc Feature(13)1190..1192(+)
Misc Feature(14)1241..1270(+)
Misc Feature(15)1349..2116(+)
Misc Feature(16)1364..2113(+)
Misc Feature(17)1382..1969(+)
Misc Feature(18)1382..1786(+)
Misc Feature(19)1580..1597(+)
Misc Feature(20)1721..1969(+)
Misc Feature(21)1772..1846(+)
Misc Feature(22)1811..1813(+)
Misc Feature(23)1901..1924(+)
Exon (1)1..130
Gene:Btk
Gene Synonym:
Exon (2)131..301
Gene:Btk
Gene Synonym:
Exon (3)302..400
Gene:Btk
Gene Synonym:
Exon (4)401..469
Gene:Btk
Gene Synonym:
Exon (5)470..551
Gene:Btk
Gene Synonym:
Exon (6)552..680
Gene:Btk
Gene Synonym:
Exon (7)681..748
Gene:Btk
Gene Synonym:
Exon (8)749..936
Gene:Btk
Gene Synonym:
Exon (9)937..999
Gene:Btk
Gene Synonym:
Exon (10)1000..1054
Gene:Btk
Gene Synonym:
Exon (11)1055..1134
Gene:Btk
Gene Synonym:
Exon (12)1135..1262
Gene:Btk
Gene Synonym:
Exon (13)1263..1337
Gene:Btk
Gene Synonym:
Exon (14)1338..1509
Gene:Btk
Gene Synonym:
Exon (15)1510..1726
Gene:Btk
Gene Synonym:
Exon (16)1727..1791
Gene:Btk
Gene Synonym:
Exon (17)1792..1910
Gene:Btk
Gene Synonym:
Exon (18)1911..2068
Gene:Btk
Gene Synonym:
Exon (19)2069..2535
Gene:Btk
Gene Synonym:
Translation
Position Chain Variation Link
93 93 g, t dbSNP:224648935
131 131 c, t dbSNP:29270166
148 148 a, g dbSNP:251765675
172 172 a, g dbSNP:226033799
254 254 c, t dbSNP:29270167
406 406 a, g dbSNP:248032047
577 577 a, g dbSNP:222648223
685 685 -, t dbSNP:8251063
706 706 c, t dbSNP:8251064
905 905 c, t dbSNP:29271257
910 910 a, g dbSNP:214189288
917 917 a, c dbSNP:250429537
976 976 c, t dbSNP:260910751
1165 1165 c, t dbSNP:233402464
1218 1218 a, g dbSNP:263268603
1309 1309 c, t dbSNP:29266989
1405 1405 c, t dbSNP:259601883
1474 1474 a, g dbSNP:234021071
1531 1531 a, g dbSNP:258652746
1702 1702 a, g dbSNP:29267374
1898 1898 a, g dbSNP:229428789
1912 1912 a, g dbSNP:229552960
1930 1930 c, t dbSNP:260880868
2342 2342 a, g dbSNP:215360515
2395 2395 c, t dbSNP:247658326
2397 2397 c, t dbSNP:228645336
2411 2411 -, a dbSNP:263900020
2507 2507 -, agggg dbSNP:266153955

Target ORF information:

RefSeq Version NM_013482
Organism Mus musculus (house mouse)
Definition Mus musculus Bruton agammaglobulinemia tyrosine kinase (Btk), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013482

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGGCTGCAG TGATACTGGA GAGCATCTTT CTGAAGCGCT CCCAGCAGAA AAAGAAAACA 
TCACCTTTAA ACTTCAAGAA GCGCCTGTTT CTCTTGACTG TACACAAACT TTCATACTAT
GAATATGACT TTGAACGTGG GAGAAGAGGC AGTAAGAAAG GTTCAATAGA TGTTGAGAAG
ATCACCTGTG TTGAAACAGT AATTCCTGAA AAAAATCCCC CACCAGAAAG ACAGATTCCG
AGGAGAGGTG AGGAGTCTAG TGAAATGGAA CAGATTTCAA TCATTGAAAG GTTCCCGTAC
CCATTCCAGG TTGTATATGA TGAAGGACCT CTCTATGTTT TCTCCCCAAC TGAAGAGCTG
AGAAAGCGCT GGATTCACCA GCTCAAAAAT GTAATCCGGT ACAATAGTGA CCTGGTACAG
AAATACCATC CTTGCTTCTG GATTGATGGA CAGTATCTCT GCTGCTCTCA GACAGCCAAG
AATGCTATGG GCTGCCAAAT TTTGGAGAAC AGGAATGGAA GCTTAAAACC TGGGAGTTCT
CATCGAAAAA CGAAAAAGCC TCTTCCCCCT ACCCCAGAGG AAGATCAGAT CTTGAAAAAA
CCGCTTCCCC CGGAGCCAAC AGCAGCACCA ATCTCCACAA CCGAGCTGAA AAAGGTCGTG
GCCCTTTATG ATTACATGCC AATGAACGCA AATGACTTAC AATTGCGAAA GGGCGAGGAG
TATTTTATCC TGGAGGAGAG CAACCTACCG TGGTGGCGAG CACGAGATAA AAATGGGCAG
GAAGGCTACA TCCCAAGTAA CTATATCACT GAAGCTGAGG ACTCCATAGA GATGTATGAG
TGGTATTCCA AGCACATGAC TCGAAGTCAA GCTGAGCAAC TGCTAAAGCA AGAGGGGAAA
GAAGGAGGTT TCATTGTCAG AGACTCCAGC AAAGCTGGAA AATACACCGT GTCTGTGTTT
GCTAAATCTA CTGGGGAGCC TCAAGGGGTG ATCCGCCATT ACGTTGTGTG TTCCACGCCA
CAGAGCCAGT ATTACCTGGC TGAGAAACAC CTCTTCAGCA CCATCCCTGA GCTCATTAAC
TACCATCAAC ACAACTCTGC AGGCCTCATA TCCAGGCTGA AATATCCTGT GTCTAAACAA
AACAAAAACG CGCCTTCTAC TGCAGGCCTG GGCTATGGAT CATGGGAAAT TGATCCAAAG
GACCTCACCT TCTTGAAGGA GCTTGGGACT GGACAATTCG GTGTCGTGAA ATATGGGAAG
TGGAGGGGCC AATATGATGT GGCCATCAAG ATGATCAGAG AAGGTTCCAT GTCGGAGGAT
GAATTCATTG AAGAAGCCAA AGTCATGATG AATCTTTCCC ATGAGAAGCT GGTGCAGTTG
TATGGCGTCT GCACCAAACA ACGCCCCATC TTCATCATCA CCGAGTACAT GGCTAATGGC
TGCCTCTTGA ACTACCTGAG GGAGATGCGG CACCGCTTCC AGACACAGCA GCTGCTTGAG
ATGTGCAAAG ATGTCTGTGA AGCAATGGAA TACTTGGAGT CGAAGCAGTT CCTTCACAGA
GACCTGGCAG CTCGAAACTG TTTGGTAAAC GATCAAGGAG TTGTGAAAGT ATCTGACTTT
GGCCTGTCTA GGTATGTCCT TGATGATGAG TACACCAGCT CTGTAGGCTC CAAGTTTCCA
GTCCGGTGGT CTCCACCAGA AGTGCTTATG TATAGCAAGT TCAGCAGCAA ATCTGACATC
TGGGCTTTTG GGGTTTTAAT GTGGGAGATC TACTCCCTGG GGAAGATGCC GTATGAGAGA
TTTACTAACA GTGAGACAGC AGAACACATT GCTCAAGGCT TACGTCTCTA CAGGCCTCAT
CTGGCATCAG AGAGGGTATA TACCATCATG TACAGCTGCT GGCACGAGAA AGCAGATGAA
CGTCCTAGTT TCAAAATTCT CTTGAGTAAC ATTCTAGATG TGATGGATGA AGAATCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.