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Gsta4 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Gsta4
Entrez Gene ID 14860
Full Name glutathione S-transferase, alpha 4
Synonyms GST5.7, mGsta4
General protein information
Preferred Names
glutathione S-transferase A4
Names
glutathione S-transferase A4
GSTA4-4
GST A4-4
GST class-alpha member 4
glutathione S-transferase 5.7
NP_034487.2
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

9

9|9 E1

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_010357 NP_034487 glutathione S-transferase A4

KEGG
mmu00480 Glutathione metabolism
mmu00982 Drug metabolism - cytochrome P450
mmu00980 Metabolism of xenobiotics by cytochrome P450
mmu05204 Chemical carcinogenesis
WikiPathways
WP1251 Metapathway biotransformation
WP662 Amino Acid metabolism
BIOCYC
MOUSE_PWY-4061 glutathione-mediated detoxification


Canis lupus familiaris (dog) LOC610304 XP_005627531.1
Bos taurus (cattle) GSTA3 NP_001070580.1
Mus musculus (house mouse) Gsta4 NP_034487.2
Rattus norvegicus (Norway rat) Gsta4 NP_001100310.1
Rattus norvegicus (Norway rat) LOC100909835 XP_003750590.1
Danio rerio (zebrafish) gstal NP_998559.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100496158 XP_002934901.1

Component

ID Name Evidence
GO:0005575 cellular_component ND
GO:0005737 cytoplasm IEA

Function

ID Name Evidence
GO:0004364 glutathione transferase activity ISO
GO:0004364 glutathione transferase activity TAS
GO:0008144 drug binding ISO
GO:0016740 transferase activity IEA
GO:0042803 protein homodimerization activity ISO
GO:0043295 glutathione binding ISO

Process

ID Name Evidence
GO:0006749 glutathione metabolic process ISO
GO:0006805 xenobiotic metabolic process ISO
GO:0008152 metabolic process IEA
GO:0008152 metabolic process TAS

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (knock-out) (1)  1 citation
  • Gene trapped (1) 

The following Gsta4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gsta4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu06028 NM_010357 Mus musculus glutathione S-transferase, alpha 4 (Gsta4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $188.30
$269.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu06028
Clone ID Related Accession (Same CDS sequence) NM_010357
Accession Version NM_010357.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 669bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product glutathione S-transferase A4
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BU953110.1, AK019271.1, AK010098.1, AK008400.1 and AI043064.1. On Nov 14, 2007 this sequence version replaced gi:142349705. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L06047.1, AK008189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCAGCCA AACCTAAGCT CTACTACTTT AATGGCAGGG GACGGATGGA GTCGATCCGC 
TGGCTGCTGG CTGCGGCTGG AGTGGAGTTT GAGGAAGAAT TTCTTGAGAC AAGGGAACAG
TATGAGAAGA TGCAAAAGGA TGGACACCTG CTTTTCGGCC AAGTACCCTT GGTTGAAATC
GATGGGATGA TGCTGACACA GACCAGGGCC ATCCTCAGCT ACCTCGCTGC CAAGTACAAC
TTGTATGGGA AGGACCTGAA GGAGAGAGTC AGGATTGACA TGTATGCAGA TGGCACCCAG
GACCTGATGA TGATGATTGC CGTGGCTCCA TTTAAAACCC CCAAGGAAAA AGAGGAGAGC
TATGATTTGA TACTGTCAAG AGCTAAAACC CGTTACTTCC CAGTGTTTGA AAAGATTTTA
AAAGACCACG GAGAGGCTTT TCTCGTTGGC AACCAGCTCA GTTGGGCAGA CATCCAGCTC
CTAGAAGCCA TTTTGATGGT GGAAGAACTC AGTGCCCCTG TACTGTCCGA CTTCCCTCTG
CTGCAGGCAT TTAAGACAAG AATCAGCAAC ATTCCTACAA TTAAGAAGTT CCTGCAACCC
GGAAGTCAGA GGAAGCCTCC TCCAGATGGC CCCTATGTTG AGGTGGTCAG GACTGTCCTG
AAGTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_034487.2
CDS120..788
Misc Feature(1)96..98(+)
Misc Feature(2)120..713(+)
Misc Feature(3)129..365(+)
Misc Feature(4)150..338(+)
Misc Feature(5)252..323(+)
Misc Feature(6)273..353(+)
Misc Feature(7)279..284(+)
Misc Feature(8)318..323(+)
Misc Feature(9)375..779(+)
Misc Feature(10)375..512(+)
Misc Feature(11)408..722(+)
Misc Feature(12)420..779(+)
Exon (1)1..99
Gene:Gsta4
Gene Synonym:
Exon (2)100..206
Gene:Gsta4
Gene Synonym:
Exon (3)207..258
Gene:Gsta4
Gene Synonym:
Exon (4)259..391
Gene:Gsta4
Gene Synonym:
Exon (5)392..533
Gene:Gsta4
Gene Synonym:
Exon (6)534..665
Gene:Gsta4
Gene Synonym:
Exon (7)666..958
Gene:Gsta4
Gene Synonym:
Translation
Position Chain Variation Link
15 15 c, t dbSNP:8261583
26 26 a, t dbSNP:8261584
29 29 a, c dbSNP:8261585
61 61 -, tt dbSNP:233171577
63 63 -, tc dbSNP:214764540
65 65 -, tcg dbSNP:221908308
66 66 -, cg, g, t dbSNP:8261586
66 66 c, g dbSNP:261061776
82 82 a, c dbSNP:29689095
111 111 c, g dbSNP:8261600
116 116 a, g dbSNP:220612861
161 161 c, t dbSNP:8261599
212 212 a, g dbSNP:8261615
260 260 c, t dbSNP:8261629
437 437 a, t dbSNP:8261639
484 484 a, c dbSNP:8261640
556 556 a, c dbSNP:213051376
641 641 a, g dbSNP:231606294
752 752 c, t dbSNP:8261682
798 798 -, t dbSNP:261788332
801 801 -, t dbSNP:8261683
814 814 a, g dbSNP:8261684
836 836 a, g dbSNP:8261685
951 951 a, g dbSNP:8261686

Target ORF information:

RefSeq Version NM_010357
Organism Mus musculus (house mouse)
Definition Mus musculus glutathione S-transferase, alpha 4 (Gsta4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_010357

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCAGCCA AACCTAAGCT CTACTACTTT AATGGCAGGG GACGGATGGA GTCGATCCGC 
TGGCTGCTGG CTGCGGCTGG AGTGGAGTTT GAGGAAGAAT TTCTTGAGAC AAGGGAACAG
TATGAGAAGA TGCAAAAGGA TGGACACCTG CTTTTCGGCC AAGTACCCTT GGTTGAAATC
GATGGGATGA TGCTGACACA GACCAGGGCC ATCCTCAGCT ACCTCGCTGC CAAGTACAAC
TTGTATGGGA AGGACCTGAA GGAGAGAGTC AGGATTGACA TGTATGCAGA TGGCACCCAG
GACCTGATGA TGATGATTGC CGTGGCTCCA TTTAAAACCC CCAAGGAAAA AGAGGAGAGC
TATGATTTGA TACTGTCAAG AGCTAAAACC CGTTACTTCC CAGTGTTTGA AAAGATTTTA
AAAGACCACG GAGAGGCTTT TCTCGTTGGC AACCAGCTCA GTTGGGCAGA CATCCAGCTC
CTAGAAGCCA TTTTGATGGT GGAAGAACTC AGTGCCCCTG TACTGTCCGA CTTCCCTCTG
CTGCAGGCAT TTAAGACAAG AATCAGCAAC ATTCCTACAA TTAAGAAGTT CCTGCAACCC
GGAAGTCAGA GGAAGCCTCC TCCAGATGGC CCCTATGTTG AGGTGGTCAG GACTGTCCTG
AAGTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Loss of glutathione S-transferase A4 accelerates obstruction-induced tubule damage and renal fibrosis
J. Pathol. (2012) In press
Liang A, Wang Y, Woodard LE, Wilson MH, Sharma R, Awasthi YC, Du J, Mitch WE and Cheng J.


book

Defective transcription initiation causes postnatal growth failure in a mouse model of nucleotide excision repair (NER) progeria
Proc. Natl. Acad. Sci. U.S.A. 109 (8), 2995-3000 (2012)
Kamileri I, Karakasilioti I, Sideri A, Kosteas T, Tatarakis A, Talianidis I and Garinis GA.


book

Down-regulation of glutatione S-transferase alpha 4 (hGSTA4) in the muscle of thermally injured patients is indicative of susceptibility to bacterial infection
FASEB J. 26 (2), 730-737 (2012)
Apidianakis Y, Que YA, Xu W, Tegos GP, Zimniak P, Hamblin MR, Tompkins RG, Xiao W and Rahme LG.


book

A high-resolution anatomical atlas of the transcriptome in the mouse embryo
PLoS Biol. 9 (1), E1000582 (2011)
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, Nurnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Dolle P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G and Ballabio A.


book

Evidence that Gsta4 modifies susceptibility to skin tumor development in mice and humans
J. Natl. Cancer Inst. 102 (21), 1663-1675 (2010)
Abel EL, Angel JM, Riggs PK, Langfield L, Lo HH, Person MD, Awasthi YC, Wang LE, Strom SS, Wei Q and DiGiovanni J.


book

Characterization of genes which exhibit reduced expression during the retinoic acid-induced differentiation of F9 teratocarcinoma cells: involvement of cyclin D3 in RA-mediated growth arrest
Mol. Cell. Endocrinol. 143 (1-2), 155-166 (1998)
Faria TN, LaRosa GJ, Wilen E, Liao J and Gudas LJ.


book

Crystal structure of a murine alpha-class glutathione S-transferase involved in cellular defense against oxidative stress
FEBS Lett. 422 (3), 285-290 (1998)
Krengel U, Schroter KH, Hoier H, Arkema A, Kalk KH, Zimniak P and Dijkstra BW.


book

Estimation of genomic complexity, heterologous expression, and enzymatic characterization of mouse glutathione S-transferase mGSTA4-4 (GST 5.7)
J. Biol. Chem. 269 (2), 992-1000 (1994)
Zimniak P, Singhal SS, Srivastava SK, Awasthi S, Sharma R, Hayden JB and Awasthi YC.


book

A subgroup of class alpha glutathione S-transferases. Cloning of cDNA for mouse lung glutathione S-transferase GST 5.7
FEBS Lett. 313 (2), 173-176 (1992)
Zimniak P, Eckles MA, Saxena M and Awasthi YC.


book

Characterization of a novel glutathione S-transferase isoenzyme from mouse lung and liver having structural similarity to rat glutathione S-transferase 8-8
Biochem. J. 278 (PT 3), 793-799 (1991)
Medh RD, Saxena M, Singhal SS, Ahmad H and Awasthi YC.


 
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