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Il10 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Il10
Entrez Gene ID 16153
Full Name interleukin 10
Synonyms CSIF, Il-10
General protein information
Preferred Names
interleukin-10
Names
interleukin-10
cytokine synthesis inhibitory factor
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

1

1 E4|1 56.89 cM

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_010548 NP_034678 interleukin-10 precursor

KEGG
mmu04060 Cytokine-cytokine receptor interaction
mmu04630 Jak-STAT signaling pathway
mmu04660 T cell receptor signaling pathway
mmu05310 Asthma
mmu05322 Systemic lupus erythematosus
mmu05330 Allograft rejection
mmu05320 Autoimmune thyroid disease
mmu04672 Intestinal immune network for IgA production
mmu05142 Chagas disease (American trypanosomiasis)
mmu05140 Leishmaniasis
mmu05146 Amoebiasis
mmu05145 Toxoplasmosis
mmu05144 Malaria
mmu05150 Staphylococcus aureus infection
mmu05143 African trypanosomiasis
mmu05152 Tuberculosis
mmu05133 Pertussis
mmu05169 Epstein-Barr virus infection
mmu04068 FoxO signaling pathway
mmu05321 Inflammatory bowel disease (IBD)
WikiPathways
WP222 Cytokines and Inflammatory Response (BioCarta)
WP1266 SIDS Susceptibility Pathways


Homo sapiens (human) IL10 NP_000563.1
Pan troglodytes (chimpanzee) IL10 NP_001129092.2
Macaca mulatta (Rhesus monkey) IL10 NP_001038192.1
Canis lupus familiaris (dog) IL10 NP_001003077.1
Bos taurus (cattle) IL10 NP_776513.1
Mus musculus (house mouse) Il10 NP_034678.1
Rattus norvegicus (Norway rat) Il10 NP_036986.2
Gallus gallus (chicken) IL10 NP_001004414.2
Danio rerio (zebrafish) il10 NP_001018621.2
Xenopus (Silurana) tropicalis (western clawed frog) il10 NP_001165400.1

Component

ID Name Evidence
GO:0005576 extracellular region ISO
GO:0005615 extracellular space IDA
GO:0005615 extracellular space ISO

Function

ID Name Evidence
GO:0005125 cytokine activity IDA
GO:0005125 cytokine activity IMP
GO:0005141 interleukin-10 receptor binding ISO

Process

ID Name Evidence
GO:0001818 negative regulation of cytokine production ISO
GO:0002237 response to molecule of bacterial origin ISO
GO:0002740 negative regulation of cytokine secretion involved in immune response ISO
GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus IMP
GO:0002904 positive regulation of B cell apoptosis ISO
GO:0006954 inflammatory response ISO
GO:0006955 immune response ISO
GO:0010468 regulation of gene expression ISO
GO:0030886 negative regulation of myeloid dendritic cell activation IMP
GO:0030889 negative regulation of B cell proliferation ISO
GO:0032689 negative regulation of interferon-gamma production IMP
GO:0032695 negative regulation of interleukin-12 production IMP
GO:0032715 negative regulation of interleukin-6 production ISO
GO:0032720 negative regulation of tumor necrosis factor production IMP
GO:0032800 receptor biosynthetic process ISO
GO:0042536 negative regulation of tumor necrosis factor biosynthetic process ISO
GO:0042742 defense response to bacterium IDA
GO:0043066 negative regulation of apoptosis ISO
GO:0044130 negative regulation of growth of symbiont in host IMP
GO:0045019 negative regulation of nitric oxide biosynthetic process ISO
GO:0045348 positive regulation of MHC class II biosynthetic process IDA
GO:0050715 positive regulation of cytokine secretion ISO
GO:0050728 negative regulation of inflammatory response ISO
GO:0051045 negative regulation of membrane protein ectodomain proteolysis ISO
GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity ISO
GO:0051384 response to glucocorticoid stimulus ISO
GO:0051930 regulation of sensory perception of pain ISO
GO:0060670 branching involved in embryonic placenta morphogenesis IMP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Il10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu22605
NM_010548 Mus musculus interleukin 10 (Il10), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$69.30
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee that the protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OMu22605
Accession Version NM_010548.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product interleukin-10 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK152344.1 and M37897.1. On Mar 26, 2010 this sequence version replaced gi:6754317. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK152344.1, BC120612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
ATGCCTGGCT CAGCACTGCT ATGCTGCCTG CTCTTACTGA CTGGCATGAG GATCAGCAGG 
GGCCAGTACA GCCGGGAAGA CAATAACTGC ACCCACTTCC CAGTCGGCCA GAGCCACATG
CTCCTAGAGC TGCGGACTGC CTTCAGCCAG GTGAAGACTT TCTTTCAAAC AAAGGACCAG
CTGGACAACA TACTGCTAAC CGACTCCTTA ATGCAGGACT TTAAGGGTTA CTTGGGTTGC
CAAGCCTTAT CGGAAATGAT CCAGTTTTAC CTGGTAGAAG TGATGCCCCA GGCAGAGAAG
CATGGCCCAG AAATCAAGGA GCATTTGAAT TCCCTGGGTG AGAAGCTGAA GACCCTCAGG
ATGCGGCTGA GGCGCTGTCA TCGATTTCTC CCCTGTGAAA ATAAGAGCAA GGCAGTGGAG
CAGGTGAAGA GTGATTTTAA TAAGCTCCAA GACCAAGGTG TCTACAAGGC CATGAATGAA
TTTGACATCT TCATCAACTG CATAGAAGCA TACATGATGA TCAAAATGAA AAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_034678.1
CDS68..604
Misc Feature(1)110..589(+)
Exon (1)1..232
Gene:Il10
Gene Synonym:
Exon (2)233..292
Gene:Il10
Gene Synonym:
Exon (3)293..445
Gene:Il10
Gene Synonym:
Exon (4)446..511
Gene:Il10
Gene Synonym:
Exon (5)512..1306
Gene:Il10
Gene Synonym:
Translation
Position Chain Variation Link
3 3 -, c dbSNP:239246505
39 39 a, g dbSNP:33489109
127 127 a, g dbSNP:249148905
268 268 c, t dbSNP:234061068
319 319 a, g dbSNP:33486912
425 425 a, c dbSNP:33486910
437 437 a, c dbSNP:235533675
472 472 a, g dbSNP:33484832
638 638 a, c dbSNP:33491366
688 688 a, g dbSNP:33491364
728 728 a, g dbSNP:33490442
860 860 c, t dbSNP:213764316
879 879 -, agtatttg dbSNP:248516980
982 982 c, t dbSNP:237083398
983 983 c, g dbSNP:33490440
1085 1085 c, g, t dbSNP:33490438
1139 1139 a, c dbSNP:244839954
1141 1141 c, t dbSNP:252572916
1230 1230 g, t dbSNP:222441344
1248 1248 a, g dbSNP:6235683
1303 1303 c, t dbSNP:257736873
1306 1306 a, g dbSNP:229748340

Target ORF information:

RefSeq Version NM_010548
Organism Mus musculus (house mouse)
Definition Mus musculus interleukin 10 (Il10), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_010548

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGCCTGGCT CAGCACTGCT ATGCTGCCTG CTCTTACTGA CTGGCATGAG GATCAGCAGG 
GGCCAGTACA GCCGGGAAGA CAATAACTGC ACCCACTTCC CAGTCGGCCA GAGCCACATG
CTCCTAGAGC TGCGGACTGC CTTCAGCCAG GTGAAGACTT TCTTTCAAAC AAAGGACCAG
CTGGACAACA TACTGCTAAC CGACTCCTTA ATGCAGGACT TTAAGGGTTA CTTGGGTTGC
CAAGCCTTAT CGGAAATGAT CCAGTTTTAC CTGGTAGAAG TGATGCCCCA GGCAGAGAAG
CATGGCCCAG AAATCAAGGA GCATTTGAAT TCCCTGGGTG AGAAGCTGAA GACCCTCAGG
ATGCGGCTGA GGCGCTGTCA TCGATTTCTC CCCTGTGAAA ATAAGAGCAA GGCAGTGGAG
CAGGTGAAGA GTGATTTTAA TAAGCTCCAA GACCAAGGTG TCTACAAGGC CATGAATGAA
TTTGACATCT TCATCAACTG CATAGAAGCA TACATGATGA TCAAAATGAA AAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Induction of TGF-beta and IL-10 production in dendritic cells using astilbin to inhibit dextran sulfate sodium-induced colitis
Biochem. Biophys. Res. Commun. 446 (2), 529-534 (2014)
Ding Y, Liang Y, Deng B, Qiao A, Wu K, Xiao W and Gong W.


book

Preferential production of IgG1, IL-4 and IL-10 in MuSK-immunized mice
Clin. Immunol. 151 (2), 155-163 (2014)
Ulusoy C, Kim E, Tuzun E, Huda R, Yilmaz V, Poulas K, Trakas N, Skriapa L, Niarchos A, Strait RT, Finkelman FD, Turan S, Zisimopoulou P, Tzartos S, Saruhan-Direskeneli G and Christadoss P.


book

S100A8 induces IL-10 and protects against acute lung injury
J. Immunol. 192 (6), 2800-2811 (2014)
Hiroshima Y, Hsu K, Tedla N, Chung YM, Chow S, Herbert C and Geczy CL.


book

Protection against collagen-induced arthritis in mice afforded by the parasitic worm product, ES-62, is associated with restoration of the levels of interleukin-10-producing B cells and reduced plasma cell infiltration of the joints
Immunology 141 (3), 457-466 (2014)
Rodgers DT, Pineda MA, McGrath MA, Al-Riyami L, Harnett W and Harnett MM.


book

[Pseudomonas aeruginosa recombinant proteins: effect on mice cytokine profile]
Zh. Mikrobiol. Epidemiol. Immunobiol. 6, 80-87 (2013)
Soldatenkova,A.V., Geiderova,L.A., Akhmatova,N.K. and Mikhailova,N.A.


book

IL-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of Trichinella spiralis-infected mice
J. Immunol. 149 (6), 2123-2129 (1992)
Ghildyal N, McNeil HP, Stechschulte S, Austen KF, Silberstein D, Gurish MF, Somerville LL and Stevens RL.


book

Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes
J. Immunol. 148 (11), 3618-3623 (1992)
Kim JM, Brannan CI, Copeland NG, Jenkins NA, Khan TA and Moore KW.


book

Interleukins 9, 10, 11 and 12 and kit ligand: a brief overview
Res. Immunol. 143 (4), 385-400 (1992)
Quesniaux VF.


book

Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI
Proc. Natl. Acad. Sci. U.S.A. 88 (4), 1172-1176 (1991)
Vieira P, de Waal-Malefyt R, Dang MN, Johnson KE, Kastelein R, Fiorentino DF, deVries JE, Roncarolo MG, Mosmann TR and Moore KW.


book

Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI
Science 248 (4960), 1230-1234 (1990)
Moore KW, Vieira P, Fiorentino DF, Trounstine ML, Khan TA and Mosmann TR.


 
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