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Ins2 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Ins2
Entrez Gene ID 16334
Full Name insulin II
Synonyms AA986540, Ins-2, InsII, Mody, Mody4
General protein information
Preferred Names
insulin-2
Names
insulin-2
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

7

7 F5|7 88.0 cM

Summary This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_008387 NP_032413 insulin-2 preproprotein
NM_001185083 NP_001172012 insulin-2 preproprotein
NM_001185084 NP_001172013 insulin-2 preproprotein



Homo sapiens (human) INS NP_000198.1
Pan troglodytes (chimpanzee) INS NP_001008996.1
Canis lupus familiaris (dog) INS NP_001123565.1
Mus musculus (house mouse) Ins2 NP_032413.1
Rattus norvegicus (Norway rat) Ins2 NP_062003.1
Gallus gallus (chicken) INS NP_990553.1
Danio rerio (zebrafish) ins NP_571131.1
Xenopus (Silurana) tropicalis (western clawed frog) ins NP_001093706.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Ins2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ins2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu22987 NM_008387 Mus musculus insulin II (Ins2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu22987 NM_001185083 Mus musculus insulin II (Ins2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu22987 NM_001185084 Mus musculus insulin II (Ins2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu22987
Clone ID Related Accession (Same CDS sequence) NM_001185083 , NM_001185084 , NM_008387
Accession Version NM_008387.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-AUG-2015
Organism Mus musculus (house mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AK007612.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced gi:297374792. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ127829.1, AK007612.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_032413.1
CDS92..424
Misc Feature(1)11..13(+)
Misc Feature(2)167..421(+)
Misc Feature(3)185..415(+)
Exon (1)1..65
Gene:Ins2
Gene Synonym:
Exon (2)66..278
Gene:Ins2
Gene Synonym:
Exon (3)279..481
Gene:Ins2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_008387
Organism Mus musculus (house mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_008387

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22987
Clone ID Related Accession (Same CDS sequence) NM_001185083 , NM_001185084 , NM_008387
Accession Version NM_001185083.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-AUG-2015
Organism Mus musculus (house mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced gi:297374793. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF581924.1, BG310959.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001172012.1
CDS80..412
Misc Feature(1)11..13(+)
Misc Feature(2)155..409(+)
Misc Feature(3)173..403(+)
Exon (1)1..65
Gene:Ins2
Gene Synonym:
Exon (2)66..266
Gene:Ins2
Gene Synonym:
Exon (3)267..469
Gene:Ins2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001185083
Organism Mus musculus (house mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185083

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22987
Clone ID Related Accession (Same CDS sequence) NM_001185083 , NM_001185084 , NM_008387
Accession Version NM_001185084.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-AUG-2015
Organism Mus musculus (house mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AY899305.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced gi:297374795. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI900780.1, AY899305.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001172013.1
CDS194..526
Misc Feature(1)125..127(+)
Misc Feature(2)269..523(+)
Misc Feature(3)287..517(+)
Exon (1)1..380
Gene:Ins2
Gene Synonym:
Exon (2)381..583
Gene:Ins2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001185084
Organism Mus musculus (house mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185084

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Low TGFbeta1 expression prevents and high expression exacerbates diabetic nephropathy in mice
Proc. Natl. Acad. Sci. U.S.A. 112 (18), 5815-5820 (2015)
Hathaway CK, Gasim AM, Grant R, Chang AS, Kim HS, Madden VJ, Bagnell CR Jr, Jennette JC, Smithies O and Kakoki M.


book

Hnf1b controls pancreas morphogenesis and the generation of Ngn3+ endocrine progenitors
Development 142 (5), 871-882 (2015)
De Vas MG, Kopp JL, Heliot C, Sander M, Cereghini S and Haumaitre C.


book

Bicaudal C1 promotes pancreatic NEUROG3+ endocrine progenitor differentiation and ductal morphogenesis
Development 142 (5), 858-870 (2015)
Lemaire LA, Goulley J, Kim YH, Carat S, Jacquemin P, Rougemont J, Constam DB and Grapin-Botton A.


book

Novel splice variant of mouse insulin2 mRNA: implications for insulin expression
FEBS Lett. 584 (6), 1169-1173 (2010)
Panda AC, Kulkarni SD, Muralidharan B, Bakthavachalu B and Seshadri V.


book

Increased insulin translation from an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance
Mol. Endocrinol. 19 (3), 794-803 (2005)
Minn AH, Lan H, Rabaglia ME, Harlan DM, Peculis BA, Attie AD and Shalev A.


book

Physical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2
Nat. Genet. 2 (1), 61-65 (1992)
Zemel S, Bartolomei MS and Tilghman SM.


book

Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos
Genes Dev. 6 (6), 939-952 (1992)
Rappolee DA, Sturm KS, Behrendtsen O, Schultz GA, Pedersen RA and Werb Z.


book

Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus
J. Mol. Endocrinol. 5 (1), 61-67 (1990)
Sawa T, Ohgaku S, Morioka H and Yano S.


book

Characterization of the two nonallelic genes encoding mouse preproinsulin
J. Mol. Evol. 23 (4), 305-312 (1986)
Wentworth,B.M., Schaefer,I.M., Villa-Komaroff,L. and Chirgwin,J.M.


book

Amino acid sequence of the two insulins from mouse (Maus musculus)
Hoppe-Seyler's Z. Physiol. Chem. 353 (3), 451-458 (1972)
Bunzli,H.F., Glatthaar,B., Kunz,P., Mulhaupt,E. and Humbel,R.E.


 
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