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Ins2 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Ins2
Entrez Gene ID 16334
Full Name insulin II
Synonyms AA986540, Ins-2, InsII, Mody, Mody4
General protein information
Preferred Names
insulin-2
Names
insulin-2
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

7

7 F5|7 88.0 cM

Summary This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_008387 NP_032413 insulin-2 preproprotein
NM_001185083 NP_001172012 insulin-2 preproprotein
NM_001185084 NP_001172013 insulin-2 preproprotein

KEGG
mmu04150 mTOR signaling pathway
mmu04140 Regulation of autophagy
mmu04810 Regulation of actin cytoskeleton
mmu04910 Insulin signaling pathway
mmu04940 Type I diabetes mellitus
mmu05215 Prostate cancer
mmu04930 Type II diabetes mellitus
mmu04950 Maturity onset diabetes of the young
mmu04960 Aldosterone-regulated sodium reabsorption
mmu04114 Oocyte meiosis
mmu04914 Progesterone-mediated oocyte maturation
mmu04911 Insulin secretion
mmu04151 PI3K-Akt signaling pathway
mmu04913 Ovarian steroidogenesis
mmu04066 HIF-1 signaling pathway
mmu04068 FoxO signaling pathway
mmu04022 cGMP-PKG signaling pathway
mmu04152 AMPK signaling pathway
mmu04917 Prolactin signaling pathway
mmu04923 Regulation of lipolysis in adipocytes
mmu04932 Non-alcoholic fatty liver disease (NAFLD)
mmu04014 Ras signaling pathway
mmu04015 Rap1 signaling pathway
WikiPathways
WP447 Adipogenesis
LIPID MAPS
PI3K PI3K/Akt/mTOR signaling pathway


Homo sapiens (human) INS NP_000198.1
Pan troglodytes (chimpanzee) INS NP_001008996.1
Canis lupus familiaris (dog) INS NP_001123565.1
Mus musculus (house mouse) Ins2 NP_032413.1
Rattus norvegicus (Norway rat) Ins2 NP_062003.1
Gallus gallus (chicken) INS NP_990553.1
Danio rerio (zebrafish) ins NP_571131.1
Xenopus (Silurana) tropicalis (western clawed frog) ins NP_001093706.1

Component

ID Name Evidence
GO:0005576 extracellular region ISO
GO:0005615 extracellular space ISO
GO:0005625 soluble fraction ISO
GO:0005634 nucleus IDA
GO:0005732 small nucleolar ribonucleoprotein complex IDA
GO:0005737 cytoplasm IDA
GO:0030141 stored secretory granule IDA
GO:0030141 stored secretory granule ISO
GO:0030141 stored secretory granule ISO

Function

ID Name Evidence
GO:0002020 protease binding ISO
GO:0005158 insulin receptor binding ISO
GO:0005159 insulin-like growth factor receptor binding ISO
GO:0005179 hormone activity ISO
GO:0005179 hormone activity TAS
GO:0032403 protein complex binding ISO

Process

ID Name Evidence
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IDA
GO:0000165 MAPKKK cascade ISO
GO:0000187 activation of MAPK activity IDA
GO:0002674 negative regulation of acute inflammatory response ISO
GO:0005975 carbohydrate metabolic process IEA
GO:0006006 glucose metabolic process IMP
GO:0006521 regulation of cellular amino acid metabolic process ISO
GO:0006953 acute-phase response ISO
GO:0006983 ER overload response IMP
GO:0007186 G-protein coupled receptor protein signaling pathway ISO
GO:0007257 activation of JUN kinase activity IDA
GO:0007520 myoblast fusion IGI
GO:0008284 positive regulation of cell proliferation ISO
GO:0008286 insulin receptor signaling pathway IDA
GO:0010468 regulation of gene expression IDA
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade ISO
GO:0014902 myotube differentiation IGI
GO:0015758 glucose transport ISO
GO:0015758 glucose transport TAS
GO:0016042 lipid catabolic process IDA
GO:0022898 regulation of transmembrane transporter activity ISO
GO:0032148 activation of protein kinase B activity ISO
GO:0032270 positive regulation of cellular protein metabolic process ISO
GO:0032880 regulation of protein localization ISO
GO:0033861 negative regulation of NAD(P)H oxidase activity ISO
GO:0042060 wound healing ISO
GO:0042177 negative regulation of protein catabolic process ISO
GO:0042325 regulation of phosphorylation IDA
GO:0042593 glucose homeostasis ISO
GO:0042981 regulation of apoptosis IMP
GO:0043393 regulation of protein binding IDA
GO:0043410 positive regulation of MAPKKK cascade ISO
GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity IDA
GO:0045725 positive regulation of glycogen biosynthetic process ISO
GO:0045740 positive regulation of DNA replication ISO
GO:0045818 negative regulation of glycogen catabolic process ISO
GO:0045821 positive regulation of glycolysis ISO
GO:0045840 positive regulation of mitosis ISO
GO:0045861 negative regulation of proteolysis ISO
GO:0045922 negative regulation of fatty acid metabolic process ISO
GO:0046326 positive regulation of glucose import ISO
GO:0046628 positive regulation of insulin receptor signaling pathway ISO
GO:0046631 alpha-beta T cell activation ISO
GO:0050708 regulation of protein secretion ISO
GO:0050709 negative regulation of protein secretion ISO
GO:0050715 positive regulation of cytokine secretion ISO
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation ISO
GO:0051092 positive regulation of NF-kappaB transcription factor activity ISO
GO:0051897 positive regulation of protein kinase B signaling cascade ISO
GO:0055089 fatty acid homeostasis ISO
GO:0060070 canonical Wnt receptor signaling pathway IGI
GO:0060266 negative regulation of respiratory burst involved in inflammatory response ISO
GO:0060267 positive regulation of respiratory burst ISO
GO:2000252 negative regulation of feeding behavior ISO

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Ins2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ins2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu22987 NM_008387 Mus musculus insulin II (Ins2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99.00
OMu22987 NM_001185083 Mus musculus insulin II (Ins2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99.00
OMu22987 NM_001185084 Mus musculus insulin II (Ins2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OMu22987
Accession Version NM_008387.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-AUG-2015
Organism Mus musculus (house mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AK007612.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced gi:297374792. Summary: This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BQ127829.1, AK007612.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_032413.1
CDS92..424
Misc Feature(1)11..13(+)
Misc Feature(2)167..421(+)
Misc Feature(3)185..415(+)
Exon (1)1..65
Gene:Ins2
Gene Synonym:
Exon (2)66..278
Gene:Ins2
Gene Synonym:
Exon (3)279..481
Gene:Ins2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_008387
Organism Mus musculus (house mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_008387

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22987
Accession Version NM_001185083.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-AUG-2015
Organism Mus musculus (house mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced gi:297374793. Summary: This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF581924.1, BG310959.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001172012.1
CDS80..412
Misc Feature(1)11..13(+)
Misc Feature(2)155..409(+)
Misc Feature(3)173..403(+)
Exon (1)1..65
Gene:Ins2
Gene Synonym:
Exon (2)66..266
Gene:Ins2
Gene Synonym:
Exon (3)267..469
Gene:Ins2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001185083
Organism Mus musculus (house mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185083

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22987
Accession Version NM_001185084.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 333bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 13-AUG-2015
Organism Mus musculus (house mouse)
Product insulin-2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP775562.1, AY899305.1 and BC099934.1. On Aug 13, 2015 this sequence version replaced gi:297374795. Summary: This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI900780.1, AY899305.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001172013.1
CDS194..526
Misc Feature(1)125..127(+)
Misc Feature(2)269..523(+)
Misc Feature(3)287..517(+)
Exon (1)1..380
Gene:Ins2
Gene Synonym:
Exon (2)381..583
Gene:Ins2
Gene Synonym:
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001185084
Organism Mus musculus (house mouse)
Definition Mus musculus insulin II (Ins2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001185084

ORF Insert Sequence:

1
61
121
181
241
301
ATGGCCCTGT GGATGCGCTT CCTGCCCCTG CTGGCCCTGC TCTTCCTCTG GGAGTCCCAC 
CCCACCCAGG CTTTTGTCAA GCAGCACCTT TGTGGTTCCC ACCTGGTGGA GGCTCTCTAC
CTGGTGTGTG GGGAGCGTGG CTTCTTCTAC ACACCCATGT CCCGCCGTGA AGTGGAGGAC
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGAG CAGGTGACCT TCAGACCTTG
GCACTGGAGG TGGCCCAGCA GAAGCGTGGC ATTGTAGATC AGTGCTGCAC CAGCATCTGC
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Low TGFbeta1 expression prevents and high expression exacerbates diabetic nephropathy in mice
Proc. Natl. Acad. Sci. U.S.A. 112 (18), 5815-5820 (2015)
Hathaway CK, Gasim AM, Grant R, Chang AS, Kim HS, Madden VJ, Bagnell CR Jr, Jennette JC, Smithies O and Kakoki M.


book

Hnf1b controls pancreas morphogenesis and the generation of Ngn3+ endocrine progenitors
Development 142 (5), 871-882 (2015)
De Vas MG, Kopp JL, Heliot C, Sander M, Cereghini S and Haumaitre C.


book

Bicaudal C1 promotes pancreatic NEUROG3+ endocrine progenitor differentiation and ductal morphogenesis
Development 142 (5), 858-870 (2015)
Lemaire LA, Goulley J, Kim YH, Carat S, Jacquemin P, Rougemont J, Constam DB and Grapin-Botton A.


book

Novel splice variant of mouse insulin2 mRNA: implications for insulin expression
FEBS Lett. 584 (6), 1169-1173 (2010)
Panda AC, Kulkarni SD, Muralidharan B, Bakthavachalu B and Seshadri V.


book

Increased insulin translation from an insulin splice-variant overexpressed in diabetes, obesity, and insulin resistance
Mol. Endocrinol. 19 (3), 794-803 (2005)
Minn AH, Lan H, Rabaglia ME, Harlan DM, Peculis BA, Attie AD and Shalev A.


book

Physical linkage of two mammalian imprinted genes, H19 and insulin-like growth factor 2
Nat. Genet. 2 (1), 61-65 (1992)
Zemel S, Bartolomei MS and Tilghman SM.


book

Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos
Genes Dev. 6 (6), 939-952 (1992)
Rappolee DA, Sturm KS, Behrendtsen O, Schultz GA, Pedersen RA and Werb Z.


book

Molecular cloning and DNA sequence analysis of preproinsulin genes in the NON mouse, an animal model of human non-obese, non-insulin-dependent diabetes mellitus
J. Mol. Endocrinol. 5 (1), 61-67 (1990)
Sawa T, Ohgaku S, Morioka H and Yano S.


book

Characterization of the two nonallelic genes encoding mouse preproinsulin
J. Mol. Evol. 23 (4), 305-312 (1986)
Wentworth,B.M., Schaefer,I.M., Villa-Komaroff,L. and Chirgwin,J.M.


book

Amino acid sequence of the two insulins from mouse (Maus musculus)
Hoppe-Seyler's Z. Physiol. Chem. 353 (3), 451-458 (1972)
Bunzli,H.F., Glatthaar,B., Kunz,P., Mulhaupt,E. and Humbel,R.E.


 
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