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Smad3 SMAD family member 3 [Mus musculus (house mouse)]

Gene Symbol Smad3
Entrez Gene ID 17127
Full Name SMAD family member 3
Synonyms AU022421, Madh3
General protein information
Preferred Names
mothers against decapentaplegic homolog 3
Names
mothers against decapentaplegic homolog 3
mad3
mMad3
Smad 3
MAD homolog 3
mothers against DPP homolog 3
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

9

9|9 D

Summary lac of sum
Disorder MIM:

Disorder Html:





Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Smad3 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smad3 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu68168 XM_006510819 PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319
OMu42551 XM_006510821 PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199
OMu20270 NM_016769 Mus musculus SMAD family member 3 (Smad3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OMu68168
Accession Version XM_006510819.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product mothers against decapentaplegic homolog 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568960760. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGAGCA AGTCACTGGA TGGTCGGCTG CAGGTGTCCC ATCGGAAGGG GCTCCCTCAC 
GTTATCTACT GCCGCCTGTG GCGATGGCCC GACCTGCACA GCCACCATGA ATTACGGGCC
ATGGAGCTCT GTGAGTTTGC CTTCAACATG AAGAAGGATG AAGTGTGTGT AAATCCTTAC
CACTATCAGA GAGTAGAGAC GCCAGTTCTA CCTCCAGTGT TGGTGCCACG CCACACCGAG
ATCCCGGCCG AGTTCCCCCC ACTGGATGAC TACAGCCATT CCATTCCCGA GAACACTAAC
TTCCCTGCTG GCATTGAGCC CCAGAGCAAT ATTCCAGAAA CCCCACCTCC TGGCTACCTG
AGTGAAGATG GAGAAACCAG TGACCACCAG ATGAACCACA GCATGGACGC AGGTTCTCCA
AACCTCTCCC CGAATCCGAT GTCCCCAGCA CACAATAACT TGGACCTACA GCCAGTCACC
TACTGTGAGC CGGCCTTCTG GTGCTCCATC TCCTACTACG AGCTGAACCA GCGAGTTGGG
GAGACATTCC ACGCCTCACA GCCATCCATG ACAGTAGATG GCTTCACTGA CCCCTCCAAC
TCGGAGCGCT TCTGCCTGGG CCTACTGTCC AATGTCAACC GGAATGCAGC CGTGGAACTT
ACAAGGCGAC ACATTGGGAG AGGTGTGCGG CTCTACTACA TCGGAGGGGA GGTCTTTGCG
GAGTGCCTCA GTGACAGTGC TATTTTCGTC CAGTCTCCCA ACTGCAACCA GCGCTATGGC
TGGCACCCGG CCACTGTCTG CAAGATCCCA CCAGGCTGCA ACCTGAAGAT CTTCAACAAC
CAGGAATTTG CTGCCCTCCT AGCTCAGTCT GTCAACCAGG GCTTTGAGGC TGTCTACCAG
CTGACGCGCA TGTGCACCAT CCGTATGAGC TTCGTCAAAG GCTGGGGAGC AGAGTACAGG
AGACAGACAG TGACCAGCAC CCCCTGCTGG ATTGAGCTAC ACCTGAATGG ACCCTTGCAG
TGGCTTGACA AGGTCCTCAC CCAGATGGGT TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT
TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006510882.2
CDS39..1121
Misc Feature(1)48..239(+)
Misc Feature(2)63..143(+)
Misc Feature(3)168..221(+)
Misc Feature(4)513..1085(+)
Misc Feature(5)564..1079(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006510819
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006510819

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGAGCA AGTCACTGGA TGGTCGGCTG CAGGTGTCCC ATCGGAAGGG GCTCCCTCAC 
GTTATCTACT GCCGCCTGTG GCGATGGCCC GACCTGCACA GCCACCATGA ATTACGGGCC
ATGGAGCTCT GTGAGTTTGC CTTCAACATG AAGAAGGATG AAGTGTGTGT AAATCCTTAC
CACTATCAGA GAGTAGAGAC GCCAGTTCTA CCTCCAGTGT TGGTGCCACG CCACACCGAG
ATCCCGGCCG AGTTCCCCCC ACTGGATGAC TACAGCCATT CCATTCCCGA GAACACTAAC
TTCCCTGCTG GCATTGAGCC CCAGAGCAAT ATTCCAGAAA CCCCACCTCC TGGCTACCTG
AGTGAAGATG GAGAAACCAG TGACCACCAG ATGAACCACA GCATGGACGC AGGTTCTCCA
AACCTCTCCC CGAATCCGAT GTCCCCAGCA CACAATAACT TGGACCTACA GCCAGTCACC
TACTGTGAGC CGGCCTTCTG GTGCTCCATC TCCTACTACG AGCTGAACCA GCGAGTTGGG
GAGACATTCC ACGCCTCACA GCCATCCATG ACAGTAGATG GCTTCACTGA CCCCTCCAAC
TCGGAGCGCT TCTGCCTGGG CCTACTGTCC AATGTCAACC GGAATGCAGC CGTGGAACTT
ACAAGGCGAC ACATTGGGAG AGGTGTGCGG CTCTACTACA TCGGAGGGGA GGTCTTTGCG
GAGTGCCTCA GTGACAGTGC TATTTTCGTC CAGTCTCCCA ACTGCAACCA GCGCTATGGC
TGGCACCCGG CCACTGTCTG CAAGATCCCA CCAGGCTGCA ACCTGAAGAT CTTCAACAAC
CAGGAATTTG CTGCCCTCCT AGCTCAGTCT GTCAACCAGG GCTTTGAGGC TGTCTACCAG
CTGACGCGCA TGTGCACCAT CCGTATGAGC TTCGTCAAAG GCTGGGGAGC AGAGTACAGG
AGACAGACAG TGACCAGCAC CCCCTGCTGG ATTGAGCTAC ACCTGAATGG ACCCTTGCAG
TGGCTTGACA AGGTCCTCAC CCAGATGGGT TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42551
Accession Version XM_006510821.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product mothers against decapentaplegic homolog 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568960764. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGAACCACA GCATGGACGC AGGTTCTCCA AACCTCTCCC CGAATCCGAT GTCCCCAGCA 
CACAATAACT TGGACCTACA GCCAGTCACC TACTGTGAGC CGGCCTTCTG GTGCTCCATC
TCCTACTACG AGCTGAACCA GCGAGTTGGG GAGACATTCC ACGCCTCACA GCCATCCATG
ACAGTAGATG GCTTCACTGA CCCCTCCAAC TCGGAGCGCT TCTGCCTGGG CCTACTGTCC
AATGTCAACC GGAATGCAGC CGTGGAACTT ACAAGGCGAC ACATTGGGAG AGGTGTGCGG
CTCTACTACA TCGGAGGGGA GGTCTTTGCG GAGTGCCTCA GTGACAGTGC TATTTTCGTC
CAGTCTCCCA ACTGCAACCA GCGCTATGGC TGGCACCCGG CCACTGTCTG CAAGATCCCA
CCAGGCTGCA ACCTGAAGAT CTTCAACAAC CAGGAATTTG CTGCCCTCCT AGCTCAGTCT
GTCAACCAGG GCTTTGAGGC TGTCTACCAG CTGACGCGCA TGTGCACCAT CCGTATGAGC
TTCGTCAAAG GCTGGGGAGC AGAGTACAGG AGACAGACAG TGACCAGCAC CCCCTGCTGG
ATTGAGCTAC ACCTGAATGG ACCCTTGCAG TGGCTTGACA AGGTCCTCAC CCAGATGGGT
TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT TAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006510884.1
CDS187..879
Misc Feature(1)271..843(+)
Misc Feature(2)322..837(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006510821
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006510821

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAACCACA GCATGGACGC AGGTTCTCCA AACCTCTCCC CGAATCCGAT GTCCCCAGCA 
CACAATAACT TGGACCTACA GCCAGTCACC TACTGTGAGC CGGCCTTCTG GTGCTCCATC
TCCTACTACG AGCTGAACCA GCGAGTTGGG GAGACATTCC ACGCCTCACA GCCATCCATG
ACAGTAGATG GCTTCACTGA CCCCTCCAAC TCGGAGCGCT TCTGCCTGGG CCTACTGTCC
AATGTCAACC GGAATGCAGC CGTGGAACTT ACAAGGCGAC ACATTGGGAG AGGTGTGCGG
CTCTACTACA TCGGAGGGGA GGTCTTTGCG GAGTGCCTCA GTGACAGTGC TATTTTCGTC
CAGTCTCCCA ACTGCAACCA GCGCTATGGC TGGCACCCGG CCACTGTCTG CAAGATCCCA
CCAGGCTGCA ACCTGAAGAT CTTCAACAAC CAGGAATTTG CTGCCCTCCT AGCTCAGTCT
GTCAACCAGG GCTTTGAGGC TGTCTACCAG CTGACGCGCA TGTGCACCAT CCGTATGAGC
TTCGTCAAAG GCTGGGGAGC AGAGTACAGG AGACAGACAG TGACCAGCAC CCCCTGCTGG
ATTGAGCTAC ACCTGAATGG ACCCTTGCAG TGGCTTGACA AGGTCCTCAC CCAGATGGGT
TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20270
Accession Version NM_016769.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1278bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector Document: User manual_GenEZ ORF Clone Products.pdf (pdf)
Clone information Clone Map
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags Document: MSDS_GenEZ ORF Clone Products.pdf (pdf)
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product mothers against decapentaplegic homolog 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158515.2, AK048626.1 and AK083158.1. On Jul 24, 2009 this sequence version replaced gi:141801800. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK048626.1, BC066850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGTCCA TCCTGCCCTT CACCCCCCCG ATCGTGAAGC GCCTGCTGGG TTGGAAGAAG 
GGCGAGCAGA ACGGGCAGGA GGAGAAGTGG TGCGAGAAGG CGGTCAAGAG CTTGGTGAAG
AAGCTCAAGA AGACGGGGCA GTTGGACGAG CTGGAGAAGG CCATCACCAC GCAGAACGTG
AACACCAAGT GCATTACCAT CCCCAGGTCA CTGGATGGTC GGCTGCAGGT GTCCCATCGG
AAGGGGCTCC CTCACGTTAT CTACTGCCGC CTGTGGCGAT GGCCCGACCT GCACAGCCAC
CATGAATTAC GGGCCATGGA GCTCTGTGAG TTTGCCTTCA ACATGAAGAA GGATGAAGTG
TGTGTAAATC CTTACCACTA TCAGAGAGTA GAGACGCCAG TTCTACCTCC AGTGTTGGTG
CCACGCCACA CCGAGATCCC GGCCGAGTTC CCCCCACTGG ATGACTACAG CCATTCCATT
CCCGAGAACA CTAACTTCCC TGCTGGCATT GAGCCCCAGA GCAATATTCC AGAAACCCCA
CCTCCTGGCT ACCTGAGTGA AGATGGAGAA ACCAGTGACC ACCAGATGAA CCACAGCATG
GACGCAGGTT CTCCAAACCT CTCCCCGAAT CCGATGTCCC CAGCACACAA TAACTTGGAC
CTACAGCCAG TCACCTACTG TGAGCCGGCC TTCTGGTGCT CCATCTCCTA CTACGAGCTG
AACCAGCGAG TTGGGGAGAC ATTCCACGCC TCACAGCCAT CCATGACAGT AGATGGCTTC
ACTGACCCCT CCAACTCGGA GCGCTTCTGC CTGGGCCTAC TGTCCAATGT CAACCGGAAT
GCAGCCGTGG AACTTACAAG GCGACACATT GGGAGAGGTG TGCGGCTCTA CTACATCGGA
GGGGAGGTCT TTGCGGAGTG CCTCAGTGAC AGTGCTATTT TCGTCCAGTC TCCCAACTGC
AACCAGCGCT ATGGCTGGCA CCCGGCCACT GTCTGCAAGA TCCCACCAGG CTGCAACCTG
AAGATCTTCA ACAACCAGGA ATTTGCTGCC CTCCTAGCTC AGTCTGTCAA CCAGGGCTTT
GAGGCTGTCT ACCAGCTGAC GCGCATGTGC ACCATCCGTA TGAGCTTCGT CAAAGGCTGG
GGAGCAGAGT ACAGGAGACA GACAGTGACC AGCACCCCCT GCTGGATTGA GCTACACCTG
AATGGACCCT TGCAGTGGCT TGACAAGGTC CTCACCCAGA TGGGTTCCCC GAGCATCCGC
TGTTCCAGTG TGTCTTAG
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_058049.3
CDS318..1595
Misc Feature(1)210..212(+)
Misc Feature(2)339..713(+)
Misc Feature(3)414..620(+)
Misc Feature(4)435..437(+)
Misc Feature(5)438..440(+)
Misc Feature(6)507..695(+)
Misc Feature(7)726..1010(+)
Misc Feature(8)987..1559(+)
Misc Feature(9)1038..1553(+)
Misc Feature(10)1128..1289(+)
Misc Feature(11)1581..1583(+)
Misc Feature(12)1584..1586(+)
Misc Feature(13)1590..1592(+)
Exon (1)1..523
Gene:Smad3
Gene Synonym:
Exon (2)524..717
Gene:Smad3
Gene Synonym:
Exon (3)718..849
Gene:Smad3
Gene Synonym:
Exon (4)850..924
Gene:Smad3
Gene Synonym:
Exon (5)925..975
Gene:Smad3
Gene Synonym:
Exon (6)976..1188
Gene:Smad3
Gene Synonym:
Exon (7)1189..1326
Gene:Smad3
Gene Synonym:
Exon (8)1327..1471
Gene:Smad3
Gene Synonym:
Exon (9)1472..5090
Gene:Smad3
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, t dbSNP:234430648
70 70 c, t dbSNP:46062446
91 91 a, g dbSNP:248199751
136 136 a, g dbSNP:50114326
183 183 c, g dbSNP:51779616
243 243 c, t dbSNP:239940102
283 283 c, t dbSNP:47537551
650 650 c, t dbSNP:46821318
746 746 c, t dbSNP:224094048
1007 1007 c, t dbSNP:230565216
1031 1031 c, t dbSNP:214925742
1179 1179 a, c dbSNP:253043214
1187 1187 c, t dbSNP:228862982
1190 1190 a, g dbSNP:215513753
1205 1205 c, t dbSNP:263583250
1289 1289 c, t dbSNP:47732336
1463 1463 a, g dbSNP:257278016
1520 1520 c, t dbSNP:50265627
1617 1617 c, t dbSNP:252743004
1626 1626 -, g dbSNP:239132273
1631 1631 c, t dbSNP:225405154
1793 1793 c, t dbSNP:233437818
1811 1811 a, g dbSNP:262490677
2017 2017 c, t dbSNP:247204972
2036 2036 -, c dbSNP:262198229
2043 2043 -, cca dbSNP:221423124
2088 2088 c, g dbSNP:218282945
2093 2093 c, t dbSNP:256965671
2169 2169 -, agatacggatgttcaag dbSNP:222278948
2196 2196 c, t dbSNP:242053538
2267 2267 c, t dbSNP:223552456
2279 2279 a, g dbSNP:262886154
2284 2284 a, g dbSNP:238609530
2345 2345 c, t dbSNP:222094597
2402 2402 c, t dbSNP:257707971
2427 2427 a, g dbSNP:236090921
2442 2442 g, t dbSNP:212640748
2737 2737 a, g dbSNP:246526093
2756 2756 c, t dbSNP:48174859
2772 2772 c, t dbSNP:50849732
2848 2848 -, aa dbSNP:387500326
2849 2849 -, aa dbSNP:242493468
3041 3041 c, t dbSNP:257858288
3042 3042 a, g dbSNP:232690679
3064 3064 -, c dbSNP:217487871
3129 3129 c, t dbSNP:50981158
3157 3157 a, g dbSNP:251749370
3190 3190 c, t dbSNP:224502943
3198 3198 g, t dbSNP:256933238
3209 3209 a, g dbSNP:46431631
3243 3243 c, t dbSNP:223624249
3246 3246 c, t dbSNP:263195103
3262 3262 a, g dbSNP:30125548
3290 3290 c, t dbSNP:222521778
3313 3313 c, g dbSNP:262451131
3360 3360 c, g dbSNP:47066344
3477 3477 a, g dbSNP:51478372
3514 3514 c, t dbSNP:33772831
3529 3529 c, t dbSNP:252455983
3554 3554 a, g dbSNP:234907196
3555 3555 a, t dbSNP:46231762
3583 3583 -, gag dbSNP:213661846
3660 3660 a, g dbSNP:214986224
3668 3668 c, t dbSNP:259067512
3701 3701 c, t dbSNP:237685520
3711 3711 c, t dbSNP:214242532
3945 3945 -, t dbSNP:387295215
3953 3953 -, t dbSNP:265496418
3954 3954 -, tt dbSNP:219119335
3954 3954 c, t dbSNP:238732401
4024 4024 c, t dbSNP:256763486
4207 4207 c, t dbSNP:224469311
4210 4210 c, t dbSNP:213101615
4269 4269 c, t dbSNP:47582558
4276 4276 c, t dbSNP:29988146
4277 4277 a, g dbSNP:265555755
4288 4288 c, t dbSNP:246480446
4289 4289 a, t dbSNP:230341772
4315 4315 c, t dbSNP:262760300
4440 4440 c, g dbSNP:45947624
4461 4461 a, g dbSNP:46258400
4505 4505 a, g dbSNP:252503846
4537 4537 a, g dbSNP:241461895
4652 4652 c, t dbSNP:223964286
4660 4660 c, g dbSNP:263236212
4665 4665 a, g dbSNP:50383155
4728 4728 -, g dbSNP:233141285
4761 4761 a, g dbSNP:214811020
4762 4762 c, t dbSNP:249284249
4766 4766 c, t dbSNP:230182697
4767 4767 a, g dbSNP:213258690
4775 4775 a, g dbSNP:248243795
4779 4779 c, g dbSNP:229497160
4840 4840 c, t dbSNP:48473346
4866 4866 a, c dbSNP:258524856
4869 4869 g, t dbSNP:30326434
4871 4871 g, t dbSNP:247208627
4885 4885 -, a dbSNP:232940030
4885 4885 a, t dbSNP:213661159
4887 4887 -, gatggtttgg dbSNP:253378018
5022 5022 c, t dbSNP:265294052
5073 5073 a, t dbSNP:235958886

Target ORF information:

RefSeq Version NM_016769
Organism Mus musculus (house mouse)
Definition Mus musculus SMAD family member 3 (Smad3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016769

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGTCCA TCCTGCCCTT CACCCCCCCG ATCGTGAAGC GCCTGCTGGG TTGGAAGAAG 
GGCGAGCAGA ACGGGCAGGA GGAGAAGTGG TGCGAGAAGG CGGTCAAGAG CTTGGTGAAG
AAGCTCAAGA AGACGGGGCA GTTGGACGAG CTGGAGAAGG CCATCACCAC GCAGAACGTG
AACACCAAGT GCATTACCAT CCCCAGGTCA CTGGATGGTC GGCTGCAGGT GTCCCATCGG
AAGGGGCTCC CTCACGTTAT CTACTGCCGC CTGTGGCGAT GGCCCGACCT GCACAGCCAC
CATGAATTAC GGGCCATGGA GCTCTGTGAG TTTGCCTTCA ACATGAAGAA GGATGAAGTG
TGTGTAAATC CTTACCACTA TCAGAGAGTA GAGACGCCAG TTCTACCTCC AGTGTTGGTG
CCACGCCACA CCGAGATCCC GGCCGAGTTC CCCCCACTGG ATGACTACAG CCATTCCATT
CCCGAGAACA CTAACTTCCC TGCTGGCATT GAGCCCCAGA GCAATATTCC AGAAACCCCA
CCTCCTGGCT ACCTGAGTGA AGATGGAGAA ACCAGTGACC ACCAGATGAA CCACAGCATG
GACGCAGGTT CTCCAAACCT CTCCCCGAAT CCGATGTCCC CAGCACACAA TAACTTGGAC
CTACAGCCAG TCACCTACTG TGAGCCGGCC TTCTGGTGCT CCATCTCCTA CTACGAGCTG
AACCAGCGAG TTGGGGAGAC ATTCCACGCC TCACAGCCAT CCATGACAGT AGATGGCTTC
ACTGACCCCT CCAACTCGGA GCGCTTCTGC CTGGGCCTAC TGTCCAATGT CAACCGGAAT
GCAGCCGTGG AACTTACAAG GCGACACATT GGGAGAGGTG TGCGGCTCTA CTACATCGGA
GGGGAGGTCT TTGCGGAGTG CCTCAGTGAC AGTGCTATTT TCGTCCAGTC TCCCAACTGC
AACCAGCGCT ATGGCTGGCA CCCGGCCACT GTCTGCAAGA TCCCACCAGG CTGCAACCTG
AAGATCTTCA ACAACCAGGA ATTTGCTGCC CTCCTAGCTC AGTCTGTCAA CCAGGGCTTT
GAGGCTGTCT ACCAGCTGAC GCGCATGTGC ACCATCCGTA TGAGCTTCGT CAAAGGCTGG
GGAGCAGAGT ACAGGAGACA GACAGTGACC AGCACCCCCT GCTGGATTGA GCTACACCTG
AATGGACCCT TGCAGTGGCT TGACAAGGTC CTCACCCAGA TGGGTTCCCC GAGCATCCGC
TGTTCCAGTG TGTCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.