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Smad3 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Smad3
Entrez Gene ID 17127
Full Name SMAD family member 3
Synonyms AU022421, Madh3
General protein information
Preferred Names
mothers against decapentaplegic homolog 3
Names
mothers against decapentaplegic homolog 3
mad3
mMad3
Smad 3
MAD homolog 3
mothers against DPP homolog 3
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

9

9|9 D

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
XM_006510819 XP_006510882 mothers against decapentaplegic homolog 3 isoform X1
XM_006510821 XP_006510884 mothers against decapentaplegic homolog 3 isoform X2
NM_016769 NP_058049 mothers against decapentaplegic homolog 3

KEGG
mmu04520 Adherens junction
mmu04110 Cell cycle
mmu04310 Wnt signaling pathway
mmu04350 TGF-beta signaling pathway
mmu05210 Colorectal cancer
mmu05220 Chronic myeloid leukemia
mmu05212 Pancreatic cancer
mmu05200 Pathways in cancer
mmu04144 Endocytosis
mmu05142 Chagas disease (American trypanosomiasis)
mmu05166 HTLV-I infection
mmu04390 Hippo signaling pathway
mmu04068 FoxO signaling pathway
mmu05321 Inflammatory bowel disease (IBD)
mmu04550 Signaling pathways regulating pluripotency of stem cells
mmu_M00680 TGF-beta signaling
mmu_M00681 Activin signaling
WikiPathways
WP1763 PluriNetWork
WP447 Adipogenesis
WP265 Delta-Notch Signaling Pathway
WP572 EGFR1 Signaling Pathway
WP252 Androgen Receptor Signaling Pathway
WP258 TGF-beta Receptor Signaling Pathway
WP488 Alpha6-Beta4 Integrin Signaling Pathway
WP512 Id Signaling Pathway
WP539 Wnt Signaling Pathway NetPath
WP190 Cell cycle
WP113 TGF Beta Signaling Pathway
WP273 Ovarian Infertility Genes
WP2309 XPodNet - protein-protein interactions in the podocyte expanded by STRING
WP2310 PodNet: protein-protein interactions in the podocyte
LIPID MAPS
CellCycle Cell cycle signaling pathway


Homo sapiens (human) SMAD3 NP_005893.1
Pan troglodytes (chimpanzee) SMAD3 XP_001159972.2
Macaca mulatta (Rhesus monkey) SMAD3 XP_001111262.1
Canis lupus familiaris (dog) SMAD3 NP_001164300.1
Bos taurus (cattle) SMAD3 NP_001192734.1
Mus musculus (house mouse) Smad3 NP_058049.3
Rattus norvegicus (Norway rat) Smad3 NP_037227.1
Gallus gallus (chicken) SMAD3 NP_989806.1
Danio rerio (zebrafish) smad3b NP_778258.1
Drosophila melanogaster (fruit fly) Smox NP_511079.1
Xenopus (Silurana) tropicalis (western clawed frog) smad3 NP_001008436.1

Component

ID Name Evidence
GO:0005622 intracellular IEA
GO:0005634 nucleus IDA
GO:0005634 nucleus ISO
GO:0005637 nuclear inner membrane ISO
GO:0005654 nucleoplasm ISO
GO:0005667 transcription factor complex ISO
GO:0005737 cytoplasm ISO
GO:0005829 cytosol ISO
GO:0005886 plasma membrane IDA
GO:0043234 protein complex ISO
GO:0043235 receptor complex ISO

Function

ID Name Evidence
GO:0001102 RNA polymerase II activating transcription factor binding ISO
GO:0003677 DNA binding ISO
GO:0003690 double-stranded DNA binding IDA
GO:0003700 sequence-specific DNA binding transcription factor activity IDA
GO:0003700 sequence-specific DNA binding transcription factor activity ISO
GO:0003706 ligand-regulated transcription factor activity ISO
GO:0005160 transforming growth factor beta receptor binding ISO
GO:0005515 protein binding IPI
GO:0005518 collagen binding IPI
GO:0008013 beta-catenin binding ISO
GO:0008134 transcription factor binding IPI
GO:0008134 transcription factor binding ISO
GO:0019901 protein kinase binding ISO
GO:0030618 transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity ISO
GO:0031625 ubiquitin protein ligase binding ISO
GO:0042803 protein homodimerization activity ISO
GO:0043565 sequence-specific DNA binding ISO
GO:0046332 SMAD binding ISO
GO:0046872 metal ion binding IEA
GO:0070410 co-SMAD binding ISO
GO:0070412 R-SMAD binding ISO

Process

ID Name Evidence
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IDA
GO:0000122 negative regulation of transcription from RNA polymerase II promoter ISO
GO:0001501 skeletal system development IGI
GO:0001666 response to hypoxia ISO
GO:0001701 in utero embryonic development IGI
GO:0001707 mesoderm formation IMP
GO:0001756 somitogenesis IMP
GO:0001836 release of cytochrome c from mitochondria ISO
GO:0001889 liver development IGI
GO:0001933 negative regulation of protein phosphorylation ISO
GO:0001947 heart looping IGI
GO:0002076 osteoblast development IGI
GO:0002520 immune system development IGI
GO:0006351 transcription, DNA-dependent ISO
GO:0006355 regulation of transcription, DNA-dependent IEA
GO:0006366 transcription from RNA polymerase II promoter TAS
GO:0006810 transport ISO
GO:0006917 induction of apoptosis ISO
GO:0006919 activation of caspase activity ISO
GO:0006955 immune response ISO
GO:0007050 cell cycle arrest ISO
GO:0007179 transforming growth factor beta receptor signaling pathway ISO
GO:0007179 transforming growth factor beta receptor signaling pathway TAS
GO:0007183 SMAD protein complex assembly ISO
GO:0007369 gastrulation IGI
GO:0007492 endoderm development IGI
GO:0008285 negative regulation of cell proliferation ISO
GO:0008633 activation of pro-apoptotic gene products ISO
GO:0008637 apoptotic mitochondrial changes ISO
GO:0009880 embryonic pattern specification IGI
GO:0010694 positive regulation of alkaline phosphatase activity ISO
GO:0010718 positive regulation of epithelial to mesenchymal transition ISO
GO:0016202 regulation of striated muscle tissue development IDA
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway IDA
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway ISO
GO:0019049 evasion of host defenses by virus ISO
GO:0030308 negative regulation of cell growth ISO
GO:0030335 positive regulation of cell migration ISO
GO:0030501 positive regulation of bone mineralization ISO
GO:0030878 thyroid gland development IGI
GO:0032731 positive regulation of interleukin-1 beta production ISO
GO:0032909 regulation of transforming growth factor-beta2 production ISO
GO:0032916 positive regulation of transforming growth factor-beta3 production ISO
GO:0035413 positive regulation of catenin import into nucleus ISO
GO:0042110 T cell activation IMP
GO:0042177 negative regulation of protein catabolic process ISO
GO:0042993 positive regulation of transcription factor import into nucleus ISO
GO:0043066 negative regulation of apoptosis ISO
GO:0045216 cell-cell junction organization ISO
GO:0045668 negative regulation of osteoblast differentiation IGI
GO:0045893 positive regulation of transcription, DNA-dependent IDA
GO:0045893 positive regulation of transcription, DNA-dependent ISO
GO:0045930 negative regulation of mitotic cell cycle ISO
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IDA
GO:0045944 positive regulation of transcription from RNA polymerase II promoter IGI
GO:0045944 positive regulation of transcription from RNA polymerase II promoter ISO
GO:0048340 paraxial mesoderm morphogenesis IMP
GO:0048589 developmental growth IGI
GO:0048617 embryonic foregut morphogenesis IGI
GO:0048701 embryonic cranial skeleton morphogenesis IGI
GO:0050678 regulation of epithelial cell proliferation IMP
GO:0050776 regulation of immune response IMP
GO:0050821 protein stabilization ISO
GO:0050927 positive regulation of positive chemotaxis ISO
GO:0051098 regulation of binding IDA
GO:0051496 positive regulation of stress fiber assembly ISO
GO:0051894 positive regulation of focal adhesion assembly ISO
GO:0060039 pericardium development IGI
GO:0060290 transdifferentiation ISO
GO:0070306 lens fiber cell differentiation IMP
GO:0090263 positive regulation of canonical Wnt receptor signaling pathway ISO

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Smad3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smad3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu68168 XM_006510819 PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $319.00
OMu42551 XM_006510821 PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $199.00
OMu20270
NM_016769 Mus musculus SMAD family member 3 (Smad3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$269.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OMu68168
Accession Version XM_006510819.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product mothers against decapentaplegic homolog 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568960760. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGAGCA AGTCACTGGA TGGTCGGCTG CAGGTGTCCC ATCGGAAGGG GCTCCCTCAC 
GTTATCTACT GCCGCCTGTG GCGATGGCCC GACCTGCACA GCCACCATGA ATTACGGGCC
ATGGAGCTCT GTGAGTTTGC CTTCAACATG AAGAAGGATG AAGTGTGTGT AAATCCTTAC
CACTATCAGA GAGTAGAGAC GCCAGTTCTA CCTCCAGTGT TGGTGCCACG CCACACCGAG
ATCCCGGCCG AGTTCCCCCC ACTGGATGAC TACAGCCATT CCATTCCCGA GAACACTAAC
TTCCCTGCTG GCATTGAGCC CCAGAGCAAT ATTCCAGAAA CCCCACCTCC TGGCTACCTG
AGTGAAGATG GAGAAACCAG TGACCACCAG ATGAACCACA GCATGGACGC AGGTTCTCCA
AACCTCTCCC CGAATCCGAT GTCCCCAGCA CACAATAACT TGGACCTACA GCCAGTCACC
TACTGTGAGC CGGCCTTCTG GTGCTCCATC TCCTACTACG AGCTGAACCA GCGAGTTGGG
GAGACATTCC ACGCCTCACA GCCATCCATG ACAGTAGATG GCTTCACTGA CCCCTCCAAC
TCGGAGCGCT TCTGCCTGGG CCTACTGTCC AATGTCAACC GGAATGCAGC CGTGGAACTT
ACAAGGCGAC ACATTGGGAG AGGTGTGCGG CTCTACTACA TCGGAGGGGA GGTCTTTGCG
GAGTGCCTCA GTGACAGTGC TATTTTCGTC CAGTCTCCCA ACTGCAACCA GCGCTATGGC
TGGCACCCGG CCACTGTCTG CAAGATCCCA CCAGGCTGCA ACCTGAAGAT CTTCAACAAC
CAGGAATTTG CTGCCCTCCT AGCTCAGTCT GTCAACCAGG GCTTTGAGGC TGTCTACCAG
CTGACGCGCA TGTGCACCAT CCGTATGAGC TTCGTCAAAG GCTGGGGAGC AGAGTACAGG
AGACAGACAG TGACCAGCAC CCCCTGCTGG ATTGAGCTAC ACCTGAATGG ACCCTTGCAG
TGGCTTGACA AGGTCCTCAC CCAGATGGGT TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006510882.2
CDS39..1121
Misc Feature(1)48..239(+)
Misc Feature(2)63..143(+)
Misc Feature(3)168..221(+)
Misc Feature(4)513..1085(+)
Misc Feature(5)564..1079(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006510819
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006510819

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCTGAGCA AGTCACTGGA TGGTCGGCTG CAGGTGTCCC ATCGGAAGGG GCTCCCTCAC 
GTTATCTACT GCCGCCTGTG GCGATGGCCC GACCTGCACA GCCACCATGA ATTACGGGCC
ATGGAGCTCT GTGAGTTTGC CTTCAACATG AAGAAGGATG AAGTGTGTGT AAATCCTTAC
CACTATCAGA GAGTAGAGAC GCCAGTTCTA CCTCCAGTGT TGGTGCCACG CCACACCGAG
ATCCCGGCCG AGTTCCCCCC ACTGGATGAC TACAGCCATT CCATTCCCGA GAACACTAAC
TTCCCTGCTG GCATTGAGCC CCAGAGCAAT ATTCCAGAAA CCCCACCTCC TGGCTACCTG
AGTGAAGATG GAGAAACCAG TGACCACCAG ATGAACCACA GCATGGACGC AGGTTCTCCA
AACCTCTCCC CGAATCCGAT GTCCCCAGCA CACAATAACT TGGACCTACA GCCAGTCACC
TACTGTGAGC CGGCCTTCTG GTGCTCCATC TCCTACTACG AGCTGAACCA GCGAGTTGGG
GAGACATTCC ACGCCTCACA GCCATCCATG ACAGTAGATG GCTTCACTGA CCCCTCCAAC
TCGGAGCGCT TCTGCCTGGG CCTACTGTCC AATGTCAACC GGAATGCAGC CGTGGAACTT
ACAAGGCGAC ACATTGGGAG AGGTGTGCGG CTCTACTACA TCGGAGGGGA GGTCTTTGCG
GAGTGCCTCA GTGACAGTGC TATTTTCGTC CAGTCTCCCA ACTGCAACCA GCGCTATGGC
TGGCACCCGG CCACTGTCTG CAAGATCCCA CCAGGCTGCA ACCTGAAGAT CTTCAACAAC
CAGGAATTTG CTGCCCTCCT AGCTCAGTCT GTCAACCAGG GCTTTGAGGC TGTCTACCAG
CTGACGCGCA TGTGCACCAT CCGTATGAGC TTCGTCAAAG GCTGGGGAGC AGAGTACAGG
AGACAGACAG TGACCAGCAC CCCCTGCTGG ATTGAGCTAC ACCTGAATGG ACCCTTGCAG
TGGCTTGACA AGGTCCTCAC CCAGATGGGT TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42551
Accession Version XM_006510821.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 693bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product mothers against decapentaplegic homolog 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568960764. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGAACCACA GCATGGACGC AGGTTCTCCA AACCTCTCCC CGAATCCGAT GTCCCCAGCA 
CACAATAACT TGGACCTACA GCCAGTCACC TACTGTGAGC CGGCCTTCTG GTGCTCCATC
TCCTACTACG AGCTGAACCA GCGAGTTGGG GAGACATTCC ACGCCTCACA GCCATCCATG
ACAGTAGATG GCTTCACTGA CCCCTCCAAC TCGGAGCGCT TCTGCCTGGG CCTACTGTCC
AATGTCAACC GGAATGCAGC CGTGGAACTT ACAAGGCGAC ACATTGGGAG AGGTGTGCGG
CTCTACTACA TCGGAGGGGA GGTCTTTGCG GAGTGCCTCA GTGACAGTGC TATTTTCGTC
CAGTCTCCCA ACTGCAACCA GCGCTATGGC TGGCACCCGG CCACTGTCTG CAAGATCCCA
CCAGGCTGCA ACCTGAAGAT CTTCAACAAC CAGGAATTTG CTGCCCTCCT AGCTCAGTCT
GTCAACCAGG GCTTTGAGGC TGTCTACCAG CTGACGCGCA TGTGCACCAT CCGTATGAGC
TTCGTCAAAG GCTGGGGAGC AGAGTACAGG AGACAGACAG TGACCAGCAC CCCCTGCTGG
ATTGAGCTAC ACCTGAATGG ACCCTTGCAG TGGCTTGACA AGGTCCTCAC CCAGATGGGT
TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006510884.1
CDS187..879
Misc Feature(1)271..843(+)
Misc Feature(2)322..837(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006510821
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus SMAD family member 3 (Smad3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006510821

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAACCACA GCATGGACGC AGGTTCTCCA AACCTCTCCC CGAATCCGAT GTCCCCAGCA 
CACAATAACT TGGACCTACA GCCAGTCACC TACTGTGAGC CGGCCTTCTG GTGCTCCATC
TCCTACTACG AGCTGAACCA GCGAGTTGGG GAGACATTCC ACGCCTCACA GCCATCCATG
ACAGTAGATG GCTTCACTGA CCCCTCCAAC TCGGAGCGCT TCTGCCTGGG CCTACTGTCC
AATGTCAACC GGAATGCAGC CGTGGAACTT ACAAGGCGAC ACATTGGGAG AGGTGTGCGG
CTCTACTACA TCGGAGGGGA GGTCTTTGCG GAGTGCCTCA GTGACAGTGC TATTTTCGTC
CAGTCTCCCA ACTGCAACCA GCGCTATGGC TGGCACCCGG CCACTGTCTG CAAGATCCCA
CCAGGCTGCA ACCTGAAGAT CTTCAACAAC CAGGAATTTG CTGCCCTCCT AGCTCAGTCT
GTCAACCAGG GCTTTGAGGC TGTCTACCAG CTGACGCGCA TGTGCACCAT CCGTATGAGC
TTCGTCAAAG GCTGGGGAGC AGAGTACAGG AGACAGACAG TGACCAGCAC CCCCTGCTGG
ATTGAGCTAC ACCTGAATGG ACCCTTGCAG TGGCTTGACA AGGTCCTCAC CCAGATGGGT
TCCCCGAGCA TCCGCTGTTC CAGTGTGTCT TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu20270
Accession Version NM_016769.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1278bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product mothers against decapentaplegic homolog 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158515.2, AK048626.1 and AK083158.1. On Jul 24, 2009 this sequence version replaced gi:141801800. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK048626.1, BC066850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGTCCA TCCTGCCCTT CACCCCCCCG ATCGTGAAGC GCCTGCTGGG TTGGAAGAAG 
GGCGAGCAGA ACGGGCAGGA GGAGAAGTGG TGCGAGAAGG CGGTCAAGAG CTTGGTGAAG
AAGCTCAAGA AGACGGGGCA GTTGGACGAG CTGGAGAAGG CCATCACCAC GCAGAACGTG
AACACCAAGT GCATTACCAT CCCCAGGTCA CTGGATGGTC GGCTGCAGGT GTCCCATCGG
AAGGGGCTCC CTCACGTTAT CTACTGCCGC CTGTGGCGAT GGCCCGACCT GCACAGCCAC
CATGAATTAC GGGCCATGGA GCTCTGTGAG TTTGCCTTCA ACATGAAGAA GGATGAAGTG
TGTGTAAATC CTTACCACTA TCAGAGAGTA GAGACGCCAG TTCTACCTCC AGTGTTGGTG
CCACGCCACA CCGAGATCCC GGCCGAGTTC CCCCCACTGG ATGACTACAG CCATTCCATT
CCCGAGAACA CTAACTTCCC TGCTGGCATT GAGCCCCAGA GCAATATTCC AGAAACCCCA
CCTCCTGGCT ACCTGAGTGA AGATGGAGAA ACCAGTGACC ACCAGATGAA CCACAGCATG
GACGCAGGTT CTCCAAACCT CTCCCCGAAT CCGATGTCCC CAGCACACAA TAACTTGGAC
CTACAGCCAG TCACCTACTG TGAGCCGGCC TTCTGGTGCT CCATCTCCTA CTACGAGCTG
AACCAGCGAG TTGGGGAGAC ATTCCACGCC TCACAGCCAT CCATGACAGT AGATGGCTTC
ACTGACCCCT CCAACTCGGA GCGCTTCTGC CTGGGCCTAC TGTCCAATGT CAACCGGAAT
GCAGCCGTGG AACTTACAAG GCGACACATT GGGAGAGGTG TGCGGCTCTA CTACATCGGA
GGGGAGGTCT TTGCGGAGTG CCTCAGTGAC AGTGCTATTT TCGTCCAGTC TCCCAACTGC
AACCAGCGCT ATGGCTGGCA CCCGGCCACT GTCTGCAAGA TCCCACCAGG CTGCAACCTG
AAGATCTTCA ACAACCAGGA ATTTGCTGCC CTCCTAGCTC AGTCTGTCAA CCAGGGCTTT
GAGGCTGTCT ACCAGCTGAC GCGCATGTGC ACCATCCGTA TGAGCTTCGT CAAAGGCTGG
GGAGCAGAGT ACAGGAGACA GACAGTGACC AGCACCCCCT GCTGGATTGA GCTACACCTG
AATGGACCCT TGCAGTGGCT TGACAAGGTC CTCACCCAGA TGGGTTCCCC GAGCATCCGC
TGTTCCAGTG TGTCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_058049.3
CDS318..1595
Misc Feature(1)210..212(+)
Misc Feature(2)339..713(+)
Misc Feature(3)414..620(+)
Misc Feature(4)435..437(+)
Misc Feature(5)438..440(+)
Misc Feature(6)507..695(+)
Misc Feature(7)726..1010(+)
Misc Feature(8)987..1559(+)
Misc Feature(9)1038..1553(+)
Misc Feature(10)1128..1289(+)
Misc Feature(11)1581..1583(+)
Misc Feature(12)1584..1586(+)
Misc Feature(13)1590..1592(+)
Exon (1)1..523
Gene:Smad3
Gene Synonym:
Exon (2)524..717
Gene:Smad3
Gene Synonym:
Exon (3)718..849
Gene:Smad3
Gene Synonym:
Exon (4)850..924
Gene:Smad3
Gene Synonym:
Exon (5)925..975
Gene:Smad3
Gene Synonym:
Exon (6)976..1188
Gene:Smad3
Gene Synonym:
Exon (7)1189..1326
Gene:Smad3
Gene Synonym:
Exon (8)1327..1471
Gene:Smad3
Gene Synonym:
Exon (9)1472..5090
Gene:Smad3
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, t dbSNP:234430648
70 70 c, t dbSNP:46062446
91 91 a, g dbSNP:248199751
136 136 a, g dbSNP:50114326
183 183 c, g dbSNP:51779616
243 243 c, t dbSNP:239940102
283 283 c, t dbSNP:47537551
650 650 c, t dbSNP:46821318
746 746 c, t dbSNP:224094048
1007 1007 c, t dbSNP:230565216
1031 1031 c, t dbSNP:214925742
1179 1179 a, c dbSNP:253043214
1187 1187 c, t dbSNP:228862982
1190 1190 a, g dbSNP:215513753
1205 1205 c, t dbSNP:263583250
1289 1289 c, t dbSNP:47732336
1463 1463 a, g dbSNP:257278016
1520 1520 c, t dbSNP:50265627
1617 1617 c, t dbSNP:252743004
1626 1626 -, g dbSNP:239132273
1631 1631 c, t dbSNP:225405154
1793 1793 c, t dbSNP:233437818
1811 1811 a, g dbSNP:262490677
2017 2017 c, t dbSNP:247204972
2036 2036 -, c dbSNP:262198229
2043 2043 -, cca dbSNP:221423124
2088 2088 c, g dbSNP:218282945
2093 2093 c, t dbSNP:256965671
2169 2169 -, agatacggatgttcaag dbSNP:222278948
2196 2196 c, t dbSNP:242053538
2267 2267 c, t dbSNP:223552456
2279 2279 a, g dbSNP:262886154
2284 2284 a, g dbSNP:238609530
2345 2345 c, t dbSNP:222094597
2402 2402 c, t dbSNP:257707971
2427 2427 a, g dbSNP:236090921
2442 2442 g, t dbSNP:212640748
2737 2737 a, g dbSNP:246526093
2756 2756 c, t dbSNP:48174859
2772 2772 c, t dbSNP:50849732
2848 2848 -, aa dbSNP:387500326
2849 2849 -, aa dbSNP:242493468
3041 3041 c, t dbSNP:257858288
3042 3042 a, g dbSNP:232690679
3064 3064 -, c dbSNP:217487871
3129 3129 c, t dbSNP:50981158
3157 3157 a, g dbSNP:251749370
3190 3190 c, t dbSNP:224502943
3198 3198 g, t dbSNP:256933238
3209 3209 a, g dbSNP:46431631
3243 3243 c, t dbSNP:223624249
3246 3246 c, t dbSNP:263195103
3262 3262 a, g dbSNP:30125548
3290 3290 c, t dbSNP:222521778
3313 3313 c, g dbSNP:262451131
3360 3360 c, g dbSNP:47066344
3477 3477 a, g dbSNP:51478372
3514 3514 c, t dbSNP:33772831
3529 3529 c, t dbSNP:252455983
3554 3554 a, g dbSNP:234907196
3555 3555 a, t dbSNP:46231762
3583 3583 -, gag dbSNP:213661846
3660 3660 a, g dbSNP:214986224
3668 3668 c, t dbSNP:259067512
3701 3701 c, t dbSNP:237685520
3711 3711 c, t dbSNP:214242532
3945 3945 -, t dbSNP:387295215
3953 3953 -, t dbSNP:265496418
3954 3954 -, tt dbSNP:219119335
3954 3954 c, t dbSNP:238732401
4024 4024 c, t dbSNP:256763486
4207 4207 c, t dbSNP:224469311
4210 4210 c, t dbSNP:213101615
4269 4269 c, t dbSNP:47582558
4276 4276 c, t dbSNP:29988146
4277 4277 a, g dbSNP:265555755
4288 4288 c, t dbSNP:246480446
4289 4289 a, t dbSNP:230341772
4315 4315 c, t dbSNP:262760300
4440 4440 c, g dbSNP:45947624
4461 4461 a, g dbSNP:46258400
4505 4505 a, g dbSNP:252503846
4537 4537 a, g dbSNP:241461895
4652 4652 c, t dbSNP:223964286
4660 4660 c, g dbSNP:263236212
4665 4665 a, g dbSNP:50383155
4728 4728 -, g dbSNP:233141285
4761 4761 a, g dbSNP:214811020
4762 4762 c, t dbSNP:249284249
4766 4766 c, t dbSNP:230182697
4767 4767 a, g dbSNP:213258690
4775 4775 a, g dbSNP:248243795
4779 4779 c, g dbSNP:229497160
4840 4840 c, t dbSNP:48473346
4866 4866 a, c dbSNP:258524856
4869 4869 g, t dbSNP:30326434
4871 4871 g, t dbSNP:247208627
4885 4885 -, a dbSNP:232940030
4885 4885 a, t dbSNP:213661159
4887 4887 -, gatggtttgg dbSNP:253378018
5022 5022 c, t dbSNP:265294052
5073 5073 a, t dbSNP:235958886

Target ORF information:

RefSeq Version NM_016769
Organism Mus musculus (house mouse)
Definition Mus musculus SMAD family member 3 (Smad3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016769

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGTCGTCCA TCCTGCCCTT CACCCCCCCG ATCGTGAAGC GCCTGCTGGG TTGGAAGAAG 
GGCGAGCAGA ACGGGCAGGA GGAGAAGTGG TGCGAGAAGG CGGTCAAGAG CTTGGTGAAG
AAGCTCAAGA AGACGGGGCA GTTGGACGAG CTGGAGAAGG CCATCACCAC GCAGAACGTG
AACACCAAGT GCATTACCAT CCCCAGGTCA CTGGATGGTC GGCTGCAGGT GTCCCATCGG
AAGGGGCTCC CTCACGTTAT CTACTGCCGC CTGTGGCGAT GGCCCGACCT GCACAGCCAC
CATGAATTAC GGGCCATGGA GCTCTGTGAG TTTGCCTTCA ACATGAAGAA GGATGAAGTG
TGTGTAAATC CTTACCACTA TCAGAGAGTA GAGACGCCAG TTCTACCTCC AGTGTTGGTG
CCACGCCACA CCGAGATCCC GGCCGAGTTC CCCCCACTGG ATGACTACAG CCATTCCATT
CCCGAGAACA CTAACTTCCC TGCTGGCATT GAGCCCCAGA GCAATATTCC AGAAACCCCA
CCTCCTGGCT ACCTGAGTGA AGATGGAGAA ACCAGTGACC ACCAGATGAA CCACAGCATG
GACGCAGGTT CTCCAAACCT CTCCCCGAAT CCGATGTCCC CAGCACACAA TAACTTGGAC
CTACAGCCAG TCACCTACTG TGAGCCGGCC TTCTGGTGCT CCATCTCCTA CTACGAGCTG
AACCAGCGAG TTGGGGAGAC ATTCCACGCC TCACAGCCAT CCATGACAGT AGATGGCTTC
ACTGACCCCT CCAACTCGGA GCGCTTCTGC CTGGGCCTAC TGTCCAATGT CAACCGGAAT
GCAGCCGTGG AACTTACAAG GCGACACATT GGGAGAGGTG TGCGGCTCTA CTACATCGGA
GGGGAGGTCT TTGCGGAGTG CCTCAGTGAC AGTGCTATTT TCGTCCAGTC TCCCAACTGC
AACCAGCGCT ATGGCTGGCA CCCGGCCACT GTCTGCAAGA TCCCACCAGG CTGCAACCTG
AAGATCTTCA ACAACCAGGA ATTTGCTGCC CTCCTAGCTC AGTCTGTCAA CCAGGGCTTT
GAGGCTGTCT ACCAGCTGAC GCGCATGTGC ACCATCCGTA TGAGCTTCGT CAAAGGCTGG
GGAGCAGAGT ACAGGAGACA GACAGTGACC AGCACCCCCT GCTGGATTGA GCTACACCTG
AATGGACCCT TGCAGTGGCT TGACAAGGTC CTCACCCAGA TGGGTTCCCC GAGCATCCGC
TGTTCCAGTG TGTCTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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