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Myc myelocytomatosis oncogene [Mus musculus (house mouse)]

Gene Symbol Myc
Entrez Gene ID 17869
Full Name myelocytomatosis oncogene
Synonyms AU016757, Myc2, Niard, Nird, bHLHe39
General protein information
Preferred Names
myc proto-oncogene protein
Names
myc proto-oncogene protein
c-myc proto-oncogene
transcription factor p64
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

15

15 26.19 cM|15 D2-D3

Summary The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]. lac of sum
Disorder MIM:

Disorder Html:
Alleles of this type are documented at Mouse Genome Informatics  (MGI)
CloneID RefSeq Accession Definition **Vector *Turnaround time Price
OMu22878 NM_010849 Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK In stock 5-7 Starting from $99.00
OMu22762 NM_001177352 Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OMu23011 NM_001177353 Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00
OMu22762 NM_001177354 Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK Not in stock 7-9 Starting from $99.00

*Business Day
**You may select a custom vector to replace pcDNA3.1-C-(k)DYK after clone is added to cart.



CloneID OMu22878D
Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product myc proto-oncogene protein isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, BB858498.1, BC138931.1 and BE456204.1. On May 25, 2006 this sequence version replaced gi:71834864. Summary: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]. Transcript Variant: This variant (1) represents the longer transcript and encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (a, also known as c-Myc 1) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The longer isoform (a) is represented in this RefSeq. CCDS Note: This CCDS ID represents the longest c-Myc isoform, also known as c-Myc 1. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This transcript variant is supported by several transcripts, including the long mRNAs X01023.1, AK145084.1 and BC138932.1. This isoform initiates translation at a non-AUG (CUG) start codon that is well-conserved. Alternative translation initiation at a downstream AUG start codon also produces a shorter isoform, known as c-Myc 2. The shorter isoform is represented by CCDS 49615.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 1628829 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK145084.1, X01023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG CAGACAGCCA CGACGATGCC CCTCAACGTG 
AACTTCACCA ACAGGAACTA TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC
GACGAGGAAG AGAATTTCTA TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC
AGTGAGGATA TCTGGAAGAA ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC
CGCTCCGGGC TCTGCTCTCC ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA
GACGATGACG GCGGCGGTGG CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG
TTACTTGGAG GAGACATGGT GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC
ATCAAGAACA TCATCATCCA GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG
GTCTCGGAGA AGCTGGCCTC CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC
GCCCGCGGGC ACAGCGTCTG CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC
GCGTCCGAGT GCATTGACCC CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG
CCCAAATCCT GTACCTCGTC CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG
TCCTCCGAGT CCTCCCCACG GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG
CCCACCACCA GCAGCGACTC TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG
TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG AGGTCGGAGT CGGGCTCATC TCCATCCCGA
GGCCACAGCA AACCTCCGCA CAGCCCACTG GTCCTCAAGA GGTGCCACGT CTCCACTCAC
CAGCACAACT ACGCCGCACC CCCCTCCACA AGGAAGGACT ATCCAGCTGC CAAGAGGGCC
AAGTTGGACA GTGGCAGGGT CCTGAAGCAG ATCAGCAACA ACCGCAAGTG CTCCAGCCCC
AGGTCCTCAG ACACGGAGGA AAACGACAAG AGGCGGACAC ACAACGTCTT GGAACGTCAG
AGGAGGAACG AGCTGAAGCG CAGCTTTTTT GCCCTGCGTG ACCAGATCCC TGAATTGGAA
AACAACGAAA AGGCCCCCAA GGTAGTGATC CTCAAAAAAG CCACCGCCTA CATCCTGTCC
ATTCAAGCAG ACGAGCACAA GCTCACCTCT GAAAAGGACT TATTGAGGAA ACGACGAGAA
CAGTTGAAAC ACAAACTCGA ACAGCTTCGA AACTCTGGTG CATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_034979.3
CDS582..1946
Misc Feature(1)46..46(+)
Misc Feature(2)211..211(+)
Misc Feature(3)576..578(+)
Misc Feature(4)627..1661(+)
Misc Feature(5)627..629(+)
Misc Feature(6)1680..1859(+)
Misc Feature(7)1689..1802(+)
Misc Feature(8)1713..1715(+)
Misc Feature(9)1731..1856(+)
Misc Feature(10)1848..1943(+)
Exon (1)1..611
Gene:Myc
Gene Synonym:
Exon (2)612..1383
Gene:Myc
Gene Synonym:
Exon (3)1384..2399
Gene:Myc
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, t dbSNP:233217571
75 75 c, g dbSNP:260263608
178 178 a, g dbSNP:259135868
200 200 -, t dbSNP:228108495
565 565 c, t dbSNP:228319259
569 569 a, c dbSNP:13474195
773 773 a, c dbSNP:228047557
926 926 a, g dbSNP:254502813
1046 1046 a, g dbSNP:218380103
1241 1241 a, g dbSNP:234736283
1494 1494 c, t dbSNP:265999139
1847 1847 a, g dbSNP:234130144
1895 1895 a, g dbSNP:245102718
2038 2038 a, g dbSNP:215698711
2119 2119 -, aa dbSNP:259408654
2158 2158 a, g dbSNP:4230764
2200 2200 -, aaa dbSNP:238373127
2248 2248 a, c dbSNP:4230763
2351 2351 -, t dbSNP:261490542
2399 2399 a, g dbSNP:4230762

Target ORF information:

RefSeq Version NM_010849
Organism Mus musculus (house mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_010849

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG CAGACAGCCA CGACGATGCC CCTCAACGTG 
AACTTCACCA ACAGGAACTA TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC
GACGAGGAAG AGAATTTCTA TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC
AGTGAGGATA TCTGGAAGAA ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC
CGCTCCGGGC TCTGCTCTCC ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA
GACGATGACG GCGGCGGTGG CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG
TTACTTGGAG GAGACATGGT GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC
ATCAAGAACA TCATCATCCA GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG
GTCTCGGAGA AGCTGGCCTC CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC
GCCCGCGGGC ACAGCGTCTG CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC
GCGTCCGAGT GCATTGACCC CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG
CCCAAATCCT GTACCTCGTC CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG
TCCTCCGAGT CCTCCCCACG GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG
CCCACCACCA GCAGCGACTC TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG
TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG AGGTCGGAGT CGGGCTCATC TCCATCCCGA
GGCCACAGCA AACCTCCGCA CAGCCCACTG GTCCTCAAGA GGTGCCACGT CTCCACTCAC
CAGCACAACT ACGCCGCACC CCCCTCCACA AGGAAGGACT ATCCAGCTGC CAAGAGGGCC
AAGTTGGACA GTGGCAGGGT CCTGAAGCAG ATCAGCAACA ACCGCAAGTG CTCCAGCCCC
AGGTCCTCAG ACACGGAGGA AAACGACAAG AGGCGGACAC ACAACGTCTT GGAACGTCAG
AGGAGGAACG AGCTGAAGCG CAGCTTTTTT GCCCTGCGTG ACCAGATCCC TGAATTGGAA
AACAACGAAA AGGCCCCCAA GGTAGTGATC CTCAAAAAAG CCACCGCCTA CATCCTGTCC
ATTCAAGCAG ACGAGCACAA GCTCACCTCT GAAAAGGACT TATTGAGGAA ACGACGAGAA
CAGTTGAAAC ACAAACTCGA ACAGCTTCGA AACTCTGGTG CATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OMu22762D
Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product myc proto-oncogene protein isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, BB858498.1, BC138931.1 and BE456204.1. Summary: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]. Transcript Variant: This variant (1) represents the longer transcript and encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (a, also known as c-Myc 1) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq, and is 15 aa shorter, compared to isoform a. Both variants 1 and 2 can encode isoform b. CCDS Note: This CCDS ID represents the shortest c-Myc isoform, also known as c-Myc 2. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces a longer isoform, known as c-Myc 1. The longer isoform is represented by CCDS 27504.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1628829) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK145084.1, X01023.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001170823.1
CDS627..1946
Misc Feature(1)46..46(+)
Misc Feature(2)211..211(+)
Misc Feature(3)576..578(+)
Misc Feature(4)582..584(+)
Misc Feature(5)627..1661(+)
Misc Feature(6)1071..1073(+)
Misc Feature(7)1611..1613(+)
Misc Feature(8)1680..1859(+)
Misc Feature(9)1689..1802(+)
Misc Feature(10)1713..1715(+)
Misc Feature(11)1731..1856(+)
Misc Feature(12)1848..1943(+)
Misc Feature(13)1863..1928(+)
Exon (1)1..611
Gene:Myc
Gene Synonym:
Exon (2)612..1383
Gene:Myc
Gene Synonym:
Exon (3)1384..2399
Gene:Myc
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, t dbSNP:233217571
75 75 c, g dbSNP:260263608
178 178 a, g dbSNP:259135868
200 200 -, t dbSNP:228108495
565 565 c, t dbSNP:228319259
569 569 a, c dbSNP:13474195
773 773 a, c dbSNP:228047557
926 926 a, g dbSNP:254502813
1046 1046 a, g dbSNP:218380103
1241 1241 a, g dbSNP:234736283
1494 1494 c, t dbSNP:265999139
1847 1847 a, g dbSNP:234130144
1895 1895 a, g dbSNP:245102718
2038 2038 a, g dbSNP:215698711
2119 2119 -, aa dbSNP:259408654
2158 2158 a, g dbSNP:4230764
2200 2200 -, aaa dbSNP:238373127
2248 2248 a, c dbSNP:4230763
2351 2351 -, t dbSNP:261490542
2399 2399 a, g dbSNP:4230762

Target ORF information:

RefSeq Version NM_001177352
Organism Mus musculus (house mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001177352

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OMu23011D
Sequence Information ORF Nucleotide Sequence (Length: 1362bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product myc proto-oncogene protein isoform c
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, AK133952.1 and BE456204.1. Summary: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (c) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The longer isoform (c) is represented in this RefSeq, and is 1 aa shorter, compared to isoform a. CCDS Note: This CCDS ID represents a c-Myc isoform that initiates translation at a well-conserved non-AUG (CUG) start codon. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This transcript variant is supported by the long mRNA AK133952.1 and by several ESTs. Alternative translation initiation at a downstream AUG start codon also produces a shorter isoform, known as c-Myc 2. The shorter isoform is represented by CCDS 49615.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 1628829 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK133952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG ACAGCCACGA CGATGCCCCT CAACGTGAAC 
TTCACCAACA GGAACTATGA CCTCGACTAC GACTCCGTAC AGCCCTATTT CATCTGCGAC
GAGGAAGAGA ATTTCTATCA CCAGCAACAG CAGAGCGAGC TGCAGCCGCC CGCGCCCAGT
GAGGATATCT GGAAGAAATT CGAGCTGCTT CCCACCCCGC CCCTGTCCCC GAGCCGCCGC
TCCGGGCTCT GCTCTCCATC CTATGTTGCG GTCGCTACGT CCTTCTCCCC AAGGGAAGAC
GATGACGGCG GCGGTGGCAA CTTCTCCACC GCCGATCAGC TGGAGATGAT GACCGAGTTA
CTTGGAGGAG ACATGGTGAA CCAGAGCTTC ATCTGCGATC CTGACGACGA GACCTTCATC
AAGAACATCA TCATCCAGGA CTGTATGTGG AGCGGTTTCT CAGCCGCTGC CAAGCTGGTC
TCGGAGAAGC TGGCCTCCTA CCAGGCTGCG CGCAAAGACA GCACCAGCCT GAGCCCCGCC
CGCGGGCACA GCGTCTGCTC CACCTCCAGC CTGTACCTGC AGGACCTCAC CGCCGCCGCG
TCCGAGTGCA TTGACCCCTC AGTGGTCTTT CCCTACCCGC TCAACGACAG CAGCTCGCCC
AAATCCTGTA CCTCGTCCGA TTCCACGGCC TTCTCTCCTT CCTCGGACTC GCTGCTGTCC
TCCGAGTCCT CCCCACGGGC CAGCCCTGAG CCCCTAGTGC TGCATGAGGA GACACCGCCC
ACCACCAGCA GCGACTCTGA AGAAGAGCAA GAAGATGAGG AAGAAATTGA TGTGGTGTCT
GTGGAGAAGA GGCAAACCCC TGCCAAGAGG TCGGAGTCGG GCTCATCTCC ATCCCGAGGC
CACAGCAAAC CTCCGCACAG CCCACTGGTC CTCAAGAGGT GCCACGTCTC CACTCACCAG
CACAACTACG CCGCACCCCC CTCCACAAGG AAGGACTATC CAGCTGCCAA GAGGGCCAAG
TTGGACAGTG GCAGGGTCCT GAAGCAGATC AGCAACAACC GCAAGTGCTC CAGCCCCAGG
TCCTCAGACA CGGAGGAAAA CGACAAGAGG CGGACACACA ACGTCTTGGA ACGTCAGAGG
AGGAACGAGC TGAAGCGCAG CTTTTTTGCC CTGCGTGACC AGATCCCTGA ATTGGAAAAC
AACGAAAAGG CCCCCAAGGT AGTGATCCTC AAAAAAGCCA CCGCCTACAT CCTGTCCATT
CAAGCAGACG AGCACAAGCT CACCTCTGAA AAGGACTTAT TGAGGAAACG ACGAGAACAG
TTGAAACACA AACTCGAACA GCTTCGAAAC TCTGGTGCATT AA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001170824.1
CDS582..1943
Misc Feature(1)46..46(+)
Misc Feature(2)211..211(+)
Misc Feature(3)576..578(+)
Misc Feature(4)624..1658(+)
Misc Feature(5)624..626(+)
Misc Feature(6)1677..1856(+)
Misc Feature(7)1686..1799(+)
Misc Feature(8)1710..1712(+)
Misc Feature(9)1728..1853(+)
Misc Feature(10)1845..1940(+)
Exon (1)1..611
Gene:Myc
Gene Synonym:
Exon (2)612..1380
Gene:Myc
Gene Synonym:
Exon (3)1381..2396
Gene:Myc
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, t dbSNP:233217571
75 75 c, g dbSNP:260263608
178 178 a, g dbSNP:259135868
200 200 -, t dbSNP:228108495
565 565 c, t dbSNP:228319259
569 569 a, c dbSNP:13474195
770 770 a, c dbSNP:228047557
923 923 a, g dbSNP:254502813
1043 1043 a, g dbSNP:218380103
1238 1238 a, g dbSNP:234736283
1491 1491 c, t dbSNP:265999139
1844 1844 a, g dbSNP:234130144
1892 1892 a, g dbSNP:245102718
2035 2035 a, g dbSNP:215698711
2116 2116 -, aa dbSNP:259408654
2155 2155 a, g dbSNP:4230764
2197 2197 -, aaa dbSNP:238373127
2245 2245 a, c dbSNP:4230763
2348 2348 -, t dbSNP:261490542
2396 2396 a, g dbSNP:4230762

Target ORF information:

RefSeq Version NM_001177353
Organism Mus musculus (house mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001177353

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
CTGGATTTCC TTTGGGCGTT GGAAACCCCG ACAGCCACGA CGATGCCCCT CAACGTGAAC 
TTCACCAACA GGAACTATGA CCTCGACTAC GACTCCGTAC AGCCCTATTT CATCTGCGAC
GAGGAAGAGA ATTTCTATCA CCAGCAACAG CAGAGCGAGC TGCAGCCGCC CGCGCCCAGT
GAGGATATCT GGAAGAAATT CGAGCTGCTT CCCACCCCGC CCCTGTCCCC GAGCCGCCGC
TCCGGGCTCT GCTCTCCATC CTATGTTGCG GTCGCTACGT CCTTCTCCCC AAGGGAAGAC
GATGACGGCG GCGGTGGCAA CTTCTCCACC GCCGATCAGC TGGAGATGAT GACCGAGTTA
CTTGGAGGAG ACATGGTGAA CCAGAGCTTC ATCTGCGATC CTGACGACGA GACCTTCATC
AAGAACATCA TCATCCAGGA CTGTATGTGG AGCGGTTTCT CAGCCGCTGC CAAGCTGGTC
TCGGAGAAGC TGGCCTCCTA CCAGGCTGCG CGCAAAGACA GCACCAGCCT GAGCCCCGCC
CGCGGGCACA GCGTCTGCTC CACCTCCAGC CTGTACCTGC AGGACCTCAC CGCCGCCGCG
TCCGAGTGCA TTGACCCCTC AGTGGTCTTT CCCTACCCGC TCAACGACAG CAGCTCGCCC
AAATCCTGTA CCTCGTCCGA TTCCACGGCC TTCTCTCCTT CCTCGGACTC GCTGCTGTCC
TCCGAGTCCT CCCCACGGGC CAGCCCTGAG CCCCTAGTGC TGCATGAGGA GACACCGCCC
ACCACCAGCA GCGACTCTGA AGAAGAGCAA GAAGATGAGG AAGAAATTGA TGTGGTGTCT
GTGGAGAAGA GGCAAACCCC TGCCAAGAGG TCGGAGTCGG GCTCATCTCC ATCCCGAGGC
CACAGCAAAC CTCCGCACAG CCCACTGGTC CTCAAGAGGT GCCACGTCTC CACTCACCAG
CACAACTACG CCGCACCCCC CTCCACAAGG AAGGACTATC CAGCTGCCAA GAGGGCCAAG
TTGGACAGTG GCAGGGTCCT GAAGCAGATC AGCAACAACC GCAAGTGCTC CAGCCCCAGG
TCCTCAGACA CGGAGGAAAA CGACAAGAGG CGGACACACA ACGTCTTGGA ACGTCAGAGG
AGGAACGAGC TGAAGCGCAG CTTTTTTGCC CTGCGTGACC AGATCCCTGA ATTGGAAAAC
AACGAAAAGG CCCCCAAGGT AGTGATCCTC AAAAAAGCCA CCGCCTACAT CCTGTCCATT
CAAGCAGACG AGCACAAGCT CACCTCTGAA AAGGACTTAT TGAGGAAACG ACGAGAACAG
TTGAAACACA AACTCGAACA GCTTCGAAAC TCTGGTGCATT AA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.


CloneID OMu22762D
Sequence Information ORF Nucleotide Sequence (Length: 1320bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector
Clone information Clone Map
Tag on pcDNA3.1-C-(k)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product myc proto-oncogene protein isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE853266.1, AK133952.1 and BE456204.1. Summary: The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini, in human and mouse. Under conditions of stress, such as high cell densities and methionine deprivation, there is a specific and dramatic increase in the synthesis of the non-AUG initiated protein, suggesting its importance in times of adversity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This variant encodes two isoforms due to the use of alternative translation initiation codons. The longer isoform (c) is derived from a non-AUG (CUG) start codon, while a shorter isoform (b, also known as c-Myc 2) is derived from a downstream AUG start codon. The shorter isoform (b) is represented in this RefSeq, and is 15 aa shorter, compared to isoform a. Both variants 1 and 2 can encode isoform b. CCDS Note: This CCDS ID represents the shortest c-Myc isoform, also known as c-Myc 2. Evidence in the literature, including in PMIDs 1628829, 3277717 and 9032273, shows that the transcript can produce multiple isoforms due to the use of alternative translation initiation codons. This isoform initiates translation at a downstream AUG start codon. Alternative translation initiation at an upstream non-AUG (CUG) start codon produces a longer isoform, known as c-Myc 1. The longer isoform is represented by CCDS 27504.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:1628829) ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: AK133952.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA
The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.
RefSeqNP_001170825.1
CDS624..1943
Misc Feature(1)46..46(+)
Misc Feature(2)211..211(+)
Misc Feature(3)576..578(+)
Misc Feature(4)582..584(+)
Misc Feature(5)624..1658(+)
Misc Feature(6)1068..1070(+)
Misc Feature(7)1608..1610(+)
Misc Feature(8)1677..1856(+)
Misc Feature(9)1686..1799(+)
Misc Feature(10)1710..1712(+)
Misc Feature(11)1728..1853(+)
Misc Feature(12)1845..1940(+)
Misc Feature(13)1860..1925(+)
Exon (1)1..611
Gene:Myc
Gene Synonym:
Exon (2)612..1380
Gene:Myc
Gene Synonym:
Exon (3)1381..2396
Gene:Myc
Gene Synonym:
Translation
Position Chain Variation Link
25 25 c, t dbSNP:233217571
75 75 c, g dbSNP:260263608
178 178 a, g dbSNP:259135868
200 200 -, t dbSNP:228108495
565 565 c, t dbSNP:228319259
569 569 a, c dbSNP:13474195
770 770 a, c dbSNP:228047557
923 923 a, g dbSNP:254502813
1043 1043 a, g dbSNP:218380103
1238 1238 a, g dbSNP:234736283
1491 1491 c, t dbSNP:265999139
1844 1844 a, g dbSNP:234130144
1892 1892 a, g dbSNP:245102718
2035 2035 a, g dbSNP:215698711
2116 2116 -, aa dbSNP:259408654
2155 2155 a, g dbSNP:4230764
2197 2197 -, aaa dbSNP:238373127
2245 2245 a, c dbSNP:4230763
2348 2348 -, t dbSNP:261490542
2396 2396 a, g dbSNP:4230762

Target ORF information:

RefSeq Version NM_001177354
Organism Mus musculus (house mouse)
Definition Mus musculus myelocytomatosis oncogene (Myc), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1-C-(k)DYK
NM_001177354

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCCCTCA ACGTGAACTT CACCAACAGG AACTATGACC TCGACTACGA CTCCGTACAG 
CCCTATTTCA TCTGCGACGA GGAAGAGAAT TTCTATCACC AGCAACAGCA GAGCGAGCTG
CAGCCGCCCG CGCCCAGTGA GGATATCTGG AAGAAATTCG AGCTGCTTCC CACCCCGCCC
CTGTCCCCGA GCCGCCGCTC CGGGCTCTGC TCTCCATCCT ATGTTGCGGT CGCTACGTCC
TTCTCCCCAA GGGAAGACGA TGACGGCGGC GGTGGCAACT TCTCCACCGC CGATCAGCTG
GAGATGATGA CCGAGTTACT TGGAGGAGAC ATGGTGAACC AGAGCTTCAT CTGCGATCCT
GACGACGAGA CCTTCATCAA GAACATCATC ATCCAGGACT GTATGTGGAG CGGTTTCTCA
GCCGCTGCCA AGCTGGTCTC GGAGAAGCTG GCCTCCTACC AGGCTGCGCG CAAAGACAGC
ACCAGCCTGA GCCCCGCCCG CGGGCACAGC GTCTGCTCCA CCTCCAGCCT GTACCTGCAG
GACCTCACCG CCGCCGCGTC CGAGTGCATT GACCCCTCAG TGGTCTTTCC CTACCCGCTC
AACGACAGCA GCTCGCCCAA ATCCTGTACC TCGTCCGATT CCACGGCCTT CTCTCCTTCC
TCGGACTCGC TGCTGTCCTC CGAGTCCTCC CCACGGGCCA GCCCTGAGCC CCTAGTGCTG
CATGAGGAGA CACCGCCCAC CACCAGCAGC GACTCTGAAG AAGAGCAAGA AGATGAGGAA
GAAATTGATG TGGTGTCTGT GGAGAAGAGG CAAACCCCTG CCAAGAGGTC GGAGTCGGGC
TCATCTCCAT CCCGAGGCCA CAGCAAACCT CCGCACAGCC CACTGGTCCT CAAGAGGTGC
CACGTCTCCA CTCACCAGCA CAACTACGCC GCACCCCCCT CCACAAGGAA GGACTATCCA
GCTGCCAAGA GGGCCAAGTT GGACAGTGGC AGGGTCCTGA AGCAGATCAG CAACAACCGC
AAGTGCTCCA GCCCCAGGTC CTCAGACACG GAGGAAAACG ACAAGAGGCG GACACACAAC
GTCTTGGAAC GTCAGAGGAG GAACGAGCTG AAGCGCAGCT TTTTTGCCCT GCGTGACCAG
ATCCCTGAAT TGGAAAACAA CGAAAAGGCC CCCAAGGTAG TGATCCTCAA AAAAGCCACC
GCCTACATCC TGTCCATTCA AGCAGACGAG CACAAGCTCA CCTCTGAAAA GGACTTATTG
AGGAAACGAC GAGAACAGTT GAAACACAAA CTCGAACAGC TTCGAAACTC TGGTGCATAA

The stop codons will be deleted if pcDNA3.1-C-(k)DYK vector is selected.