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Rag2 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Rag2
Entrez Gene ID 19374
Full Name recombination activating gene 2
Synonyms Rag-2
General protein information
Preferred Names
V(D)J recombination-activating protein 2
Names
V(D)J recombination-activating protein 2
recombinase activating gene 2
recombination activating protein 2
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

2

2 E2|2 53.87 cM

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_009020 NP_033046 V(D)J recombination-activating protein 2

KEGG
mmu05340 Primary immunodeficiency
mmu04068 FoxO signaling pathway


Homo sapiens (human) RAG2 NP_001230714.1
Pan troglodytes (chimpanzee) RAG2 XP_521889.1
Macaca mulatta (Rhesus monkey) RAG2 XP_001114734.2
Canis lupus familiaris (dog) RAG2 XP_005631162.1
Bos taurus (cattle) RAG2 NP_001075938.1
Mus musculus (house mouse) Rag2 NP_033046.1
Rattus norvegicus (Norway rat) Rag2 NP_001093998.1
Gallus gallus (chicken) RAG2 XP_004941607.1
Danio rerio (zebrafish) rag2 NP_571460.2
Xenopus (Silurana) tropicalis (western clawed frog) rag2 XP_002937337.2

Component

ID Name Evidence
GO:0005634 nucleus IC

Function

ID Name Evidence
GO:0003677 DNA binding IEA
GO:0003682 chromatin binding IDA
GO:0005515 protein binding IPI
GO:0005546 phosphatidylinositol-4,5-bisphosphate binding IDA
GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding IDA
GO:0008270 zinc ion binding IDA
GO:0035064 methylated histone residue binding IDA
GO:0035091 phosphatidylinositol binding IDA
GO:0043325 phosphatidylinositol-3,4-bisphosphate binding IDA
GO:0046872 metal ion binding IEA
GO:0080025 phosphatidylinositol-3,5-bisphosphate binding IDA

Process

ID Name Evidence
GO:0002326 B cell lineage commitment IMP
GO:0002331 pre-B cell allelic exclusion IMP
GO:0002358 B cell homeostatic proliferation IMP
GO:0002360 T cell lineage commitment IMP
GO:0006310 DNA recombination TAS
GO:0016568 chromatin modification IEA
GO:0030183 B cell differentiation IMP
GO:0030217 T cell differentiation IMP
GO:0033077 T cell differentiation in thymus IMP
GO:0033151 V(D)J recombination IGI
GO:0033151 V(D)J recombination IMP
GO:0046622 positive regulation of organ growth IGI
GO:0046622 positive regulation of organ growth IMP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Rag2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rag2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu22385 NM_009020 Mus musculus recombination activating gene 2 (Rag2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $300.30
$429.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OMu22385
Accession Version NM_009020.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1584bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product V(D)J recombination-activating protein 2
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK040375.1. On Apr 9, 2007 this sequence version replaced gi:31543574. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK040375.1, AK031014.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164135 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGC AGATGGTAAC AGTGGGTCAT AACATAGCCT TAATTCAACC AGGCTTCTCA 
CTTATGAATT TTGATGGCCA AGTTTTCTTC TTTGGCCAGA AAGGCTGGCC TAAGAGATCC
TGTCCTACTG GAGTCTTTCA TTTTGATATA AAACAAAATC ATCTCAAACT GAAGCCTGCA
ATCTTCTCTA AAGATTCCTG CTACCTCCCA CCTCTTCGTT ATCCAGCTAC TTGCTCATAC
AAAGGCAGCA TAGACTCTGA CAAGCATCAA TATATCATTC ACGGAGGGAA AACACCAAAC
AATGAGCTTT CCGATAAGAT TTATATCATG TCTGTCGCTT GCAAGAATAA CAAAAAAGTT
ACTTTCCGTT GCACAGAGAA AGACTTAGTA GGAGATGTCC CTGAACCCAG ATACGGCCAT
TCCATTGACG TGGTGTATAG TCGAGGGAAA AGCATGGGTG TTCTCTTTGG AGGACGTTCA
TACATGCCTT CTACCCAGAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTACCC
CATGTTTTCT TGATAGATTT TGAATTTGGG TGTGCTACAT CATATATTCT CCCAGAACTT
CAGGATGGGC TGTCTTTTCA TGTTTCTATT GCCAGAAACG ATACCGTTTA TATTTTGGGA
GGACACTCAC TTGCCAGTAA TATACGCCCT GCTAACTTGT ATAGAATAAG AGTGGACCTT
CCCCTGGGTA CCCCAGCAGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTCA CTCAAACAAA CAATGATGAA TTTGTTATTG TGGGTGGTTA TCAGCTGGAA
AATCAGAAAA GGATGGTCTG CAGCCTTGTC TCTCTAGGGG ACAACACGAT TGAAATCAGT
GAGATGGAGA CTCCTGACTG GACCTCAGAT ATTAAGCATA GCAAAATATG GTTTGGAAGC
AACATGGGAA ACGGGACTAT TTTCCTTGGC ATACCAGGAG ACAATAAGCA GGCTATGTCA
GAAGCATTCT ATTTCTATAC TTTGAGATGC TCTGAAGAGG ATTTGAGTGA AGATCAGAAA
ATTGTCTCCA ACAGTCAGAC ATCAACAGAA GATCCTGGGG ACTCCACTCC CTTTGAAGAC
TCAGAGGAAT TTTGTTTCAG TGCTGAAGCA ACCAGTTTTG ATGGTGACGA TGAATTTGAC
ACCTACAATG AAGATGATGA AGATGACGAG TCTGTAACCG GCTACTGGAT AACATGTTGC
CCTACTTGTG ATGTTGACAT CAATACCTGG GTTCCGTTCT ATTCAACGGA GCTCAATAAA
CCCGCCATGA TCTATTGTTC TCATGGGGAT GGGCACTGGG TACATGCCCA GTGCATGGAT
TTGGAAGAAC GCACACTCAT CCACTTGTCA GAAGGAAGCA ACAAGTATTA TTGCAATGAA
CATGTACAGA TAGCAAGAGC ATTGCAAACT CCCAAAAGAA ACCCCCCCTT ACAAAAACCT
CCAATGAAAT CCCTCCACAA AAAAGGCTCT GGGAAAGTCT TGACTCCTGC CAAGAAATCC
TTCCTTAGAA GACTGTTTGA TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_033046.1
CDS212..1795
Misc Feature(1)188..190(+)
Misc Feature(2)362..1378(+)
Misc Feature(3)1454..1654(+)
Misc Feature(4)1463..1639(+)
Misc Feature(5)1466..1654(+)
Exon (1)1..123
Gene:Rag2
Gene Synonym:
Exon (2)124..186
Gene:Rag2
Gene Synonym:
Exon (3)187..3393
Gene:Rag2
Gene Synonym:
Translation
Position Chain Variation Link
361 361 a, g dbSNP:52008792
517 517 a, g dbSNP:243386827
646 646 a, g dbSNP:264875564
846 846 a, g dbSNP:227801537
850 850 c, t dbSNP:27362157
915 915 a, g dbSNP:27362156
1087 1087 a, g dbSNP:27362155
1153 1153 c, t dbSNP:246789438
1190 1190 a, g dbSNP:214148221
1246 1246 c, t dbSNP:237755686
1261 1261 c, t dbSNP:27362154
1285 1285 g, t dbSNP:27362153
1432 1432 a, g dbSNP:33512329
1473 1473 c, g dbSNP:27362152
1534 1534 c, t dbSNP:27362151
1669 1669 a, g dbSNP:232714188
1723 1723 c, t dbSNP:250351016
1824 1824 c, t dbSNP:27362150
1841 1841 -, at dbSNP:265671797
1842 1842 -, tt dbSNP:234437300
1860 1860 a, g dbSNP:29912172
1979 1979 a, g dbSNP:262055979
2063 2063 a, g dbSNP:228355232
2223 2223 a, t dbSNP:27362149
2232 2232 a, g dbSNP:260910584
2251 2251 -, tt dbSNP:216072381
2283 2283 a, t dbSNP:228318078
2323 2323 g, t dbSNP:246890235
2352 2352 a, g dbSNP:265355547
2383 2383 c, t dbSNP:51141914
2404 2404 a, g dbSNP:253748620
2484 2484 c, t dbSNP:51846253
2486 2486 a, g dbSNP:261943973
2487 2487 g, t dbSNP:246421798
2491 2491 c, t dbSNP:46724845
2539 2539 a, g dbSNP:232813574
2545 2545 a, t dbSNP:49192465
2597 2597 a, c dbSNP:29818547
2639 2639 c, g dbSNP:45637825
2667 2667 g, t dbSNP:258092580
2730 2730 c, t dbSNP:261636966
2753 2753 c, t dbSNP:220684314
2863 2863 a, g dbSNP:242799173
2882 2882 a, g dbSNP:260681034
2889 2889 c, t dbSNP:221974610
2903 2903 -, t dbSNP:249504159
3114 3114 -, caccc dbSNP:227587032
3146 3146 c, t dbSNP:240882161
3183 3183 g, t dbSNP:27362148
3214 3214 a, g dbSNP:230493187
3226 3226 g, t dbSNP:250164284
3285 3285 a, g dbSNP:27362147

Target ORF information:

RefSeq Version NM_009020
Organism Mus musculus (house mouse)
Definition Mus musculus recombination activating gene 2 (Rag2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_009020

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTCCCTGC AGATGGTAAC AGTGGGTCAT AACATAGCCT TAATTCAACC AGGCTTCTCA 
CTTATGAATT TTGATGGCCA AGTTTTCTTC TTTGGCCAGA AAGGCTGGCC TAAGAGATCC
TGTCCTACTG GAGTCTTTCA TTTTGATATA AAACAAAATC ATCTCAAACT GAAGCCTGCA
ATCTTCTCTA AAGATTCCTG CTACCTCCCA CCTCTTCGTT ATCCAGCTAC TTGCTCATAC
AAAGGCAGCA TAGACTCTGA CAAGCATCAA TATATCATTC ACGGAGGGAA AACACCAAAC
AATGAGCTTT CCGATAAGAT TTATATCATG TCTGTCGCTT GCAAGAATAA CAAAAAAGTT
ACTTTCCGTT GCACAGAGAA AGACTTAGTA GGAGATGTCC CTGAACCCAG ATACGGCCAT
TCCATTGACG TGGTGTATAG TCGAGGGAAA AGCATGGGTG TTCTCTTTGG AGGACGTTCA
TACATGCCTT CTACCCAGAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTACCC
CATGTTTTCT TGATAGATTT TGAATTTGGG TGTGCTACAT CATATATTCT CCCAGAACTT
CAGGATGGGC TGTCTTTTCA TGTTTCTATT GCCAGAAACG ATACCGTTTA TATTTTGGGA
GGACACTCAC TTGCCAGTAA TATACGCCCT GCTAACTTGT ATAGAATAAG AGTGGACCTT
CCCCTGGGTA CCCCAGCAGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT
GCAATCCTCA CTCAAACAAA CAATGATGAA TTTGTTATTG TGGGTGGTTA TCAGCTGGAA
AATCAGAAAA GGATGGTCTG CAGCCTTGTC TCTCTAGGGG ACAACACGAT TGAAATCAGT
GAGATGGAGA CTCCTGACTG GACCTCAGAT ATTAAGCATA GCAAAATATG GTTTGGAAGC
AACATGGGAA ACGGGACTAT TTTCCTTGGC ATACCAGGAG ACAATAAGCA GGCTATGTCA
GAAGCATTCT ATTTCTATAC TTTGAGATGC TCTGAAGAGG ATTTGAGTGA AGATCAGAAA
ATTGTCTCCA ACAGTCAGAC ATCAACAGAA GATCCTGGGG ACTCCACTCC CTTTGAAGAC
TCAGAGGAAT TTTGTTTCAG TGCTGAAGCA ACCAGTTTTG ATGGTGACGA TGAATTTGAC
ACCTACAATG AAGATGATGA AGATGACGAG TCTGTAACCG GCTACTGGAT AACATGTTGC
CCTACTTGTG ATGTTGACAT CAATACCTGG GTTCCGTTCT ATTCAACGGA GCTCAATAAA
CCCGCCATGA TCTATTGTTC TCATGGGGAT GGGCACTGGG TACATGCCCA GTGCATGGAT
TTGGAAGAAC GCACACTCAT CCACTTGTCA GAAGGAAGCA ACAAGTATTA TTGCAATGAA
CATGTACAGA TAGCAAGAGC ATTGCAAACT CCCAAAAGAA ACCCCCCCTT ACAAAAACCT
CCAATGAAAT CCCTCCACAA AAAAGGCTCT GGGAAAGTCT TGACTCCTGC CAAGAAATCC
TTCCTTAGAA GACTGTTTGA TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A committed precursor to innate lymphoid cells
Nature 508 (7496), 397-401 (2014)
Constantinides MG, McDonald BD, Verhoef PA and Bendelac A.


book

Development and function of human innate immune cells in a humanized mouse model
Nat. Biotechnol. 32 (4), 364-372 (2014)
Rongvaux A, Willinger T, Martinek J, Strowig T, Gearty SV, Teichmann LL, Saito Y, Marches F, Halene S, Palucka AK, Manz MG and Flavell RA.


book

The niche component periostin is produced by cancer-associated fibroblasts, supporting growth of gastric cancer through ERK activation
Am. J. Pathol. 184 (3), 859-870 (2014)
Kikuchi Y, Kunita A, Iwata C, Komura D, Nishiyama T, Shimazu K, Takeshita K, Shibahara J, Kii I, Morishita Y, Yashiro M, Hirakawa K, Miyazono K, Kudo A, Fukayama M and Kashima TG.


book

Gata3 drives development of RORgammat+ group 3 innate lymphoid cells
J. Exp. Med. 211 (2), 199-208 (2014)
Serafini N, Klein Wolterink RG, Satoh-Takayama N, Xu W, Vosshenrich CA, Hendriks RW and Di Santo JP.


book

Thymocyte apoptosis drives the intrathymic generation of regulatory T cells
Proc. Natl. Acad. Sci. U.S.A. 111 (4), E465-E473 (2014)
Konkel JE, Jin W, Abbatiello B, Grainger JR and Chen W.


book

Activation of V(D)J recombination by RAG1 and RAG2
Trends Genet. 8 (12), 413-416 (1992)
Oettinger MA.


book

RAG-1-deficient mice have no mature B and T lymphocytes
Cell 68 (5), 869-877 (1992)
Mombaerts P, Iacomini J, Johnson RS, Herrup K, Tonegawa S and Papaioannou VE.


book

RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
Cell 68 (5), 855-867 (1992)
Shinkai Y, Rathbun G, Lam KP, Oltz EM, Stewart V, Mendelsohn M, Charron J, Datta M, Young F and Stall AM.


book

V(D)J recombination: molecular biology and regulation
Annu. Rev. Immunol. 10, 359-383 (1992)
Schatz DG, Oettinger MA and Schlissel MS.


book

The intracisternal A particle derived solo LTR promoter of the rat oncomodulin gene is not present in the mouse gene
Genetica 86 (1-3), 85-97 (1992)
Banville D, Rotaru M and Boie Y.


 
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