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Tcf3 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Tcf3
Entrez Gene ID 21423
Full Name transcription factor 3
Synonyms A1, AA408400, ALF2, AW209082, E12, E12/E47, E2A, E47, ME2, Pan1, Pan2, TCF-3, Tcfe2a, VDIR, bHLHb21
General protein information
Preferred Names
transcription factor E2-alpha
Names
transcription factor E2-alpha
TCF-3
transcription factor A1
transcription factor E2a
immunoglobulin enhancer-binding factor E12/E47
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

10

10 C1|10 39.72 cM

Summary lac of sum
Disorder MIM:

Disorder Html:





Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Tcf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu19356 NM_001164147 Mus musculus transcription factor 3 (Tcf3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OMu19727 NM_001164148 Mus musculus transcription factor 3 (Tcf3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OMu19548 NM_001164149 Mus musculus transcription factor 3 (Tcf3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 Quote Price
OMu19304 NM_001164150 Mus musculus transcription factor 3 (Tcf3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OMu19666 NM_001164151 Mus musculus transcription factor 3 (Tcf3), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $439.00
OMu19320 NM_001164152 Mus musculus transcription factor 3 (Tcf3), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 Quote Price
OMu19599 NM_001164153 Mus musculus transcription factor 3 (Tcf3), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 Quote Price
OMu19377 NM_011548 Mus musculus transcription factor 3 (Tcf3), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 Quote Price

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OMu19356
Accession Version NM_001164147.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCCCAGAC GAGGACGAGG ACGACCTTCT CCCCCCAGAG
CAGAAGGCGG AGCGGGAGAA GGAGCGCCGG GTGGCCAATA ATGCCCGAGA GCGCCTGCGG
GTCCGCGACA TCAATGAGGC CTTTAAGGAG CTCGGCCGCA TGTGCCAGCT GCACCTCAGC
AGCGAGAAGC CGCAGACCAA ACTGCTCATC CTGCACCAGG CCGTGGCCGT CATCCTCAGC
CTGGAGCAGC AGGTGCGAGA ACGCAACCTG AACCCCAAAG CAGCCTGCTT GAAGCGGAGG
GAGGAGGAGA AGGTGTCTGG CGTGGTCGGG GACCCACAGC TGGCCCTGTC AGCCGCCCAC
CCGGGCCTGG GTGAGGCCCA CAACCCAGCC GGGCACCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157619.1
CDS392..2353
Misc Feature(1)344..346(+)
Misc Feature(2)2027..2209(+)
Misc Feature(3)2036..2152(+)
Misc Feature(4)2060..2062(+)
Misc Feature(5)2078..2206(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1714
Gene:Tcf3
Gene Synonym:
Exon (16)1715..1841
Gene:Tcf3
Gene Synonym:
Exon (17)1842..1974
Gene:Tcf3
Gene Synonym:
Exon (18)1975..2210
Gene:Tcf3
Gene Synonym:
Exon (19)2211..3320
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1609 1609 c, t dbSNP:46560563
1658 1658 a, g dbSNP:252425924
1678 1678 c, t dbSNP:248667255
1708 1708 c, g dbSNP:13475077
1792 1792 a, g dbSNP:237397282
1928 1928 a, g dbSNP:231429124
2290 2290 a, g dbSNP:263407852
2356 2356 c, t dbSNP:249607601
2499 2499 a, t dbSNP:232030199
2537 2537 a, g dbSNP:265357213
2740 2740 a, g dbSNP:47869257
2762 2762 -, t dbSNP:247375360
2813 2813 -, tt dbSNP:223730260
2813 2813 -, t dbSNP:235364184
2921 2921 a, g dbSNP:218928619
2971 2971 a, t dbSNP:248296311
3035 3035 a, g dbSNP:241738723
3098 3098 c, t dbSNP:226228845
3132 3132 a, g dbSNP:259587319
3161 3161 a, g dbSNP:33849853
3180 3180 -, gtgtgtgtgt dbSNP:265182960
3242 3242 a, t dbSNP:239928800
3243 3243 a, g dbSNP:223428091
3247 3247 a, g dbSNP:251090431
3253 3253 c, t dbSNP:230459809
3286 3286 c, t dbSNP:211961128
3308 3308 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164147
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164147

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCCCAGAC GAGGACGAGG ACGACCTTCT CCCCCCAGAG
CAGAAGGCGG AGCGGGAGAA GGAGCGCCGG GTGGCCAATA ATGCCCGAGA GCGCCTGCGG
GTCCGCGACA TCAATGAGGC CTTTAAGGAG CTCGGCCGCA TGTGCCAGCT GCACCTCAGC
AGCGAGAAGC CGCAGACCAA ACTGCTCATC CTGCACCAGG CCGTGGCCGT CATCCTCAGC
CTGGAGCAGC AGGTGCGAGA ACGCAACCTG AACCCCAAAG CAGCCTGCTT GAAGCGGAGG
GAGGAGGAGA AGGTGTCTGG CGTGGTCGGG GACCCACAGC TGGCCCTGTC AGCCGCCCAC
CCGGGCCTGG GTGAGGCCCA CAACCCAGCC GGGCACCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19727
Accession Version NM_001164148.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (2) has an alternate splice site in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC018260.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CCCAGACGAG GACGAGGACG ACCTTCTCCC CCCAGAGCAG
AAGGCGGAGC GGGAGAAGGA GCGCCGGGTG GCCAATAATG CCCGAGAGCG CCTGCGGGTC
CGCGACATCA ATGAGGCCTT TAAGGAGCTC GGCCGCATGT GCCAGCTGCA CCTCAGCAGC
GAGAAGCCGC AGACCAAACT GCTCATCCTG CACCAGGCCG TGGCCGTCAT CCTCAGCCTG
GAGCAGCAGG TGCGAGAACG CAACCTGAAC CCCAAAGCAG CCTGCTTGAA GCGGAGGGAG
GAGGAGAAGG TGTCTGGCGT GGTCGGGGAC CCACAGCTGG CCCTGTCAGC CGCCCACCCG
GGCCTGGGTG AGGCCCACAA CCCAGCCGGG CACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157620.1
CDS392..2350
Misc Feature(1)344..346(+)
Misc Feature(2)1553..1660(+)
Misc Feature(3)2024..2206(+)
Misc Feature(4)2033..2149(+)
Misc Feature(5)2057..2059(+)
Misc Feature(6)2075..2203(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2207
Gene:Tcf3
Gene Synonym:
Exon (19)2208..3317
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1606 1606 c, t dbSNP:46560563
1655 1655 a, g dbSNP:252425924
1675 1675 c, t dbSNP:248667255
1705 1705 c, g dbSNP:13475077
1789 1789 a, g dbSNP:237397282
1925 1925 a, g dbSNP:231429124
2287 2287 a, g dbSNP:263407852
2353 2353 c, t dbSNP:249607601
2496 2496 a, t dbSNP:232030199
2534 2534 a, g dbSNP:265357213
2737 2737 a, g dbSNP:47869257
2759 2759 -, t dbSNP:247375360
2810 2810 -, tt dbSNP:223730260
2810 2810 -, t dbSNP:235364184
2918 2918 a, g dbSNP:218928619
2968 2968 a, t dbSNP:248296311
3032 3032 a, g dbSNP:241738723
3095 3095 c, t dbSNP:226228845
3129 3129 a, g dbSNP:259587319
3158 3158 a, g dbSNP:33849853
3177 3177 -, gtgtgtgtgt dbSNP:265182960
3239 3239 a, t dbSNP:239928800
3240 3240 a, g dbSNP:223428091
3244 3244 a, g dbSNP:251090431
3250 3250 c, t dbSNP:230459809
3283 3283 c, t dbSNP:211961128
3305 3305 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164148
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164148

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CCCAGACGAG GACGAGGACG ACCTTCTCCC CCCAGAGCAG
AAGGCGGAGC GGGAGAAGGA GCGCCGGGTG GCCAATAATG CCCGAGAGCG CCTGCGGGTC
CGCGACATCA ATGAGGCCTT TAAGGAGCTC GGCCGCATGT GCCAGCTGCA CCTCAGCAGC
GAGAAGCCGC AGACCAAACT GCTCATCCTG CACCAGGCCG TGGCCGTCAT CCTCAGCCTG
GAGCAGCAGG TGCGAGAACG CAACCTGAAC CCCAAAGCAG CCTGCTTGAA GCGGAGGGAG
GAGGAGAAGG TGTCTGGCGT GGTCGGGGAC CCACAGCTGG CCCTGTCAGC CGCCCACCCG
GGCCTGGGTG AGGCCCACAA CCCAGCCGGG CACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19548
Accession Version NM_001164149.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (3) has two alternate splice sites in the coding region, compared to variant 1. The resulting isoform (3) lacks two internal aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK156265.1, AK017617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCCC AGACGAGGAC GAGGACGACC TTCTCCCCCC AGAGCAGAAG
GCGGAGCGGG AGAAGGAGCG CCGGGTGGCC AATAATGCCC GAGAGCGCCT GCGGGTCCGC
GACATCAATG AGGCCTTTAA GGAGCTCGGC CGCATGTGCC AGCTGCACCT CAGCAGCGAG
AAGCCGCAGA CCAAACTGCT CATCCTGCAC CAGGCCGTGG CCGTCATCCT CAGCCTGGAG
CAGCAGGTGC GAGAACGCAA CCTGAACCCC AAAGCAGCCT GCTTGAAGCG GAGGGAGGAG
GAGAAGGTGT CTGGCGTGGT CGGGGACCCA CAGCTGGCCC TGTCAGCCGC CCACCCGGGC
CTGGGTGAGG CCCACAACCC AGCCGGGCAC CTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157621.1
CDS392..2347
Misc Feature(1)344..346(+)
Misc Feature(2)1550..1657(+)
Misc Feature(3)2021..2203(+)
Misc Feature(4)2030..2146(+)
Misc Feature(5)2054..2056(+)
Misc Feature(6)2072..2200(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1708
Gene:Tcf3
Gene Synonym:
Exon (16)1709..1835
Gene:Tcf3
Gene Synonym:
Exon (17)1836..1968
Gene:Tcf3
Gene Synonym:
Exon (18)1969..2204
Gene:Tcf3
Gene Synonym:
Exon (19)2205..3314
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1603 1603 c, t dbSNP:46560563
1652 1652 a, g dbSNP:252425924
1672 1672 c, t dbSNP:248667255
1702 1702 c, g dbSNP:13475077
1786 1786 a, g dbSNP:237397282
1922 1922 a, g dbSNP:231429124
2284 2284 a, g dbSNP:263407852
2350 2350 c, t dbSNP:249607601
2493 2493 a, t dbSNP:232030199
2531 2531 a, g dbSNP:265357213
2734 2734 a, g dbSNP:47869257
2756 2756 -, t dbSNP:247375360
2807 2807 -, tt dbSNP:223730260
2807 2807 -, t dbSNP:235364184
2915 2915 a, g dbSNP:218928619
2965 2965 a, t dbSNP:248296311
3029 3029 a, g dbSNP:241738723
3092 3092 c, t dbSNP:226228845
3126 3126 a, g dbSNP:259587319
3155 3155 a, g dbSNP:33849853
3174 3174 -, gtgtgtgtgt dbSNP:265182960
3236 3236 a, t dbSNP:239928800
3237 3237 a, g dbSNP:223428091
3241 3241 a, g dbSNP:251090431
3247 3247 c, t dbSNP:230459809
3280 3280 c, t dbSNP:211961128
3302 3302 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164149
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164149

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCCC AGACGAGGAC GAGGACGACC TTCTCCCCCC AGAGCAGAAG
GCGGAGCGGG AGAAGGAGCG CCGGGTGGCC AATAATGCCC GAGAGCGCCT GCGGGTCCGC
GACATCAATG AGGCCTTTAA GGAGCTCGGC CGCATGTGCC AGCTGCACCT CAGCAGCGAG
AAGCCGCAGA CCAAACTGCT CATCCTGCAC CAGGCCGTGG CCGTCATCCT CAGCCTGGAG
CAGCAGGTGC GAGAACGCAA CCTGAACCCC AAAGCAGCCT GCTTGAAGCG GAGGGAGGAG
GAGAAGGTGT CTGGCGTGGT CGGGGACCCA CAGCTGGCCC TGTCAGCCGC CCACCCGGGC
CTGGGTGAGG CCCACAACCC AGCCGGGCAC CTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19304
Accession Version NM_001164150.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 4
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (4) has an alternate penultimate coding exon, compared to variant 1. The resulting isoform (4) has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK161009.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCAGTACA GATGAGGTGC TGTCCCTGGA GGAGAAGGAC
CTGAGGGACC GGGAGAGGCG TATGGCCAAT AACGCTCGGG AGCGGGTGCG CGTGCGGGAC
ATTAACGAGG CCTTCCGGGA GCTGGGCCGC ATGTGCCAGC TGCACCTCAA GTCGGATAAG
GCGCAGACCA AGCTGCTCAT CCTGCAGCAG GCGGTGCAGG TCATCCTGGG CCTGGAGCAG
CAGGTGCGAG AACGCAACCT GAACCCCAAA GCAGCCTGCT TGAAGCGGAG GGAGGAGGAG
AAGGTGTCTG GCGTGGTCGG GGACCCACAG CTGGCCCTGT CAGCCGCCCA CCCGGGCCTG
GGTGAGGCCC ACAACCCAGC CGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157622.1
CDS392..2344
Misc Feature(1)344..346(+)
Misc Feature(2)2018..2200(+)
Misc Feature(3)2027..2143(+)
Misc Feature(4)2051..2053(+)
Misc Feature(5)2069..2197(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1714
Gene:Tcf3
Gene Synonym:
Exon (16)1715..1841
Gene:Tcf3
Gene Synonym:
Exon (17)1842..1974
Gene:Tcf3
Gene Synonym:
Exon (18)1975..2201
Gene:Tcf3
Gene Synonym:
Exon (19)2202..3311
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1609 1609 c, t dbSNP:46560563
1658 1658 a, g dbSNP:252425924
1678 1678 c, t dbSNP:248667255
1708 1708 c, g dbSNP:13475077
1792 1792 a, g dbSNP:237397282
1928 1928 a, g dbSNP:231429124
2281 2281 a, g dbSNP:263407852
2347 2347 c, t dbSNP:249607601
2490 2490 a, t dbSNP:232030199
2528 2528 a, g dbSNP:265357213
2731 2731 a, g dbSNP:47869257
2753 2753 -, t dbSNP:247375360
2804 2804 -, tt dbSNP:223730260
2804 2804 -, t dbSNP:235364184
2912 2912 a, g dbSNP:218928619
2962 2962 a, t dbSNP:248296311
3026 3026 a, g dbSNP:241738723
3089 3089 c, t dbSNP:226228845
3123 3123 a, g dbSNP:259587319
3152 3152 a, g dbSNP:33849853
3171 3171 -, gtgtgtgtgt dbSNP:265182960
3233 3233 a, t dbSNP:239928800
3234 3234 a, g dbSNP:223428091
3238 3238 a, g dbSNP:251090431
3244 3244 c, t dbSNP:230459809
3277 3277 c, t dbSNP:211961128
3299 3299 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164150
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164150

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCAGTACA GATGAGGTGC TGTCCCTGGA GGAGAAGGAC
CTGAGGGACC GGGAGAGGCG TATGGCCAAT AACGCTCGGG AGCGGGTGCG CGTGCGGGAC
ATTAACGAGG CCTTCCGGGA GCTGGGCCGC ATGTGCCAGC TGCACCTCAA GTCGGATAAG
GCGCAGACCA AGCTGCTCAT CCTGCAGCAG GCGGTGCAGG TCATCCTGGG CCTGGAGCAG
CAGGTGCGAG AACGCAACCT GAACCCCAAA GCAGCCTGCT TGAAGCGGAG GGAGGAGGAG
AAGGTGTCTG GCGTGGTCGG GGACCCACAG CTGGCCCTGT CAGCCGCCCA CCCGGGCCTG
GGTGAGGCCC ACAACCCAGC CGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19666
Accession Version NM_001164151.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1950bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 5
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (5) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (5) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK038738.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157623.1
CDS392..2341
Misc Feature(1)344..346(+)
Misc Feature(2)2015..2197(+)
Misc Feature(3)2024..2140(+)
Misc Feature(4)2048..2050(+)
Misc Feature(5)2066..2194(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2198
Gene:Tcf3
Gene Synonym:
Exon (19)2199..3308
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1606 1606 c, t dbSNP:46560563
1655 1655 a, g dbSNP:252425924
1675 1675 c, t dbSNP:248667255
1705 1705 c, g dbSNP:13475077
1789 1789 a, g dbSNP:237397282
1925 1925 a, g dbSNP:231429124
2278 2278 a, g dbSNP:263407852
2344 2344 c, t dbSNP:249607601
2487 2487 a, t dbSNP:232030199
2525 2525 a, g dbSNP:265357213
2728 2728 a, g dbSNP:47869257
2750 2750 -, t dbSNP:247375360
2801 2801 -, tt dbSNP:223730260
2801 2801 -, t dbSNP:235364184
2909 2909 a, g dbSNP:218928619
2959 2959 a, t dbSNP:248296311
3023 3023 a, g dbSNP:241738723
3086 3086 c, t dbSNP:226228845
3120 3120 a, g dbSNP:259587319
3149 3149 a, g dbSNP:33849853
3168 3168 -, gtgtgtgtgt dbSNP:265182960
3230 3230 a, t dbSNP:239928800
3231 3231 a, g dbSNP:223428091
3235 3235 a, g dbSNP:251090431
3241 3241 c, t dbSNP:230459809
3274 3274 c, t dbSNP:211961128
3296 3296 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164151
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19320
Accession Version NM_001164152.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1950bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 6
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (6) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (6) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157624.1
CDS392..2341
Misc Feature(1)344..346(+)
Misc Feature(2)2015..2197(+)
Misc Feature(3)2024..2140(+)
Misc Feature(4)2048..2050(+)
Misc Feature(5)2066..2194(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2198
Gene:Tcf3
Gene Synonym:
Exon (19)2199..3308
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1606 1606 c, t dbSNP:46560563
1655 1655 a, g dbSNP:252425924
1675 1675 c, t dbSNP:248667255
1705 1705 c, g dbSNP:13475077
1789 1789 a, g dbSNP:237397282
1925 1925 a, g dbSNP:231429124
2278 2278 a, g dbSNP:263407852
2344 2344 c, t dbSNP:249607601
2487 2487 a, t dbSNP:232030199
2525 2525 a, g dbSNP:265357213
2728 2728 a, g dbSNP:47869257
2750 2750 -, t dbSNP:247375360
2801 2801 -, tt dbSNP:223730260
2801 2801 -, t dbSNP:235364184
2909 2909 a, g dbSNP:218928619
2959 2959 a, t dbSNP:248296311
3023 3023 a, g dbSNP:241738723
3086 3086 c, t dbSNP:226228845
3120 3120 a, g dbSNP:259587319
3149 3149 a, g dbSNP:33849853
3168 3168 -, gtgtgtgtgt dbSNP:265182960
3230 3230 a, t dbSNP:239928800
3231 3231 a, g dbSNP:223428091
3235 3235 a, g dbSNP:251090431
3241 3241 c, t dbSNP:230459809
3274 3274 c, t dbSNP:211961128
3296 3296 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164152
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164152

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19599
Accession Version NM_001164153.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 7
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (7) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (7) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK034777.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_001157625.1
CDS392..2338
Misc Feature(1)344..346(+)
Misc Feature(2)2012..2194(+)
Misc Feature(3)2021..2137(+)
Misc Feature(4)2045..2047(+)
Misc Feature(5)2063..2191(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2195
Gene:Tcf3
Gene Synonym:
Exon (19)2196..3305
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1606 1606 c, t dbSNP:46560563
1655 1655 a, g dbSNP:252425924
1675 1675 c, t dbSNP:248667255
1705 1705 c, g dbSNP:13475077
1789 1789 a, g dbSNP:237397282
1925 1925 a, g dbSNP:231429124
2275 2275 a, g dbSNP:263407852
2341 2341 c, t dbSNP:249607601
2484 2484 a, t dbSNP:232030199
2522 2522 a, g dbSNP:265357213
2725 2725 a, g dbSNP:47869257
2747 2747 -, t dbSNP:247375360
2798 2798 -, tt dbSNP:223730260
2798 2798 -, t dbSNP:235364184
2906 2906 a, g dbSNP:218928619
2956 2956 a, t dbSNP:248296311
3020 3020 a, g dbSNP:241738723
3083 3083 c, t dbSNP:226228845
3117 3117 a, g dbSNP:259587319
3146 3146 a, g dbSNP:33849853
3165 3165 -, gtgtgtgtgt dbSNP:265182960
3227 3227 a, t dbSNP:239928800
3228 3228 a, g dbSNP:223428091
3232 3232 a, g dbSNP:251090431
3238 3238 c, t dbSNP:230459809
3271 3271 c, t dbSNP:211961128
3293 3293 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_001164153
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164153

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19377
Accession Version NM_011548.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 8
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. On Aug 13, 2009 this sequence version replaced gi:46402510. Transcript Variant: This variant (8) has two alternate splice sites and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (8) lacks two internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006860.1, AF352579.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_035678.3
CDS392..2338
Misc Feature(1)344..346(+)
Misc Feature(2)2012..2194(+)
Misc Feature(3)2021..2137(+)
Misc Feature(4)2045..2047(+)
Misc Feature(5)2063..2191(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1708
Gene:Tcf3
Gene Synonym:
Exon (16)1709..1835
Gene:Tcf3
Gene Synonym:
Exon (17)1836..1968
Gene:Tcf3
Gene Synonym:
Exon (18)1969..2195
Gene:Tcf3
Gene Synonym:
Exon (19)2196..3305
Gene:Tcf3
Gene Synonym:
Translation
Position Chain Variation Link
155 155 a, g dbSNP:233028840
348 348 c, t dbSNP:13480663
475 475 a, g dbSNP:261495515
1603 1603 c, t dbSNP:46560563
1652 1652 a, g dbSNP:252425924
1672 1672 c, t dbSNP:248667255
1702 1702 c, g dbSNP:13475077
1786 1786 a, g dbSNP:237397282
1922 1922 a, g dbSNP:231429124
2275 2275 a, g dbSNP:263407852
2341 2341 c, t dbSNP:249607601
2484 2484 a, t dbSNP:232030199
2522 2522 a, g dbSNP:265357213
2725 2725 a, g dbSNP:47869257
2747 2747 -, t dbSNP:247375360
2798 2798 -, tt dbSNP:223730260
2798 2798 -, t dbSNP:235364184
2906 2906 a, g dbSNP:218928619
2956 2956 a, t dbSNP:248296311
3020 3020 a, g dbSNP:241738723
3083 3083 c, t dbSNP:226228845
3117 3117 a, g dbSNP:259587319
3146 3146 a, g dbSNP:33849853
3165 3165 -, gtgtgtgtgt dbSNP:265182960
3227 3227 a, t dbSNP:239928800
3228 3228 a, g dbSNP:223428091
3232 3232 a, g dbSNP:251090431
3238 3238 c, t dbSNP:230459809
3271 3271 c, t dbSNP:211961128
3293 3293 g, t dbSNP:248534810

Target ORF information:

RefSeq Version NM_011548
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_011548

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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