×

Tcf3 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Tcf3
Entrez Gene ID 21423
Full Name transcription factor 3
Synonyms A1, AA408400, ALF2, AW209082, E12, E12/E47, E2A, E47, ME2, Pan1, Pan2, TCF-3, Tcfe2a, VDIR, bHLHb21
General protein information
Preferred Names
transcription factor E2-alpha
Names
transcription factor E2-alpha
TCF-3
transcription factor A1
transcription factor E2a
immunoglobulin enhancer-binding factor E12/E47
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

10

10 C1|10 39.72 cM

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_001164147 NP_001157619 transcription factor E2-alpha isoform 1
NM_001164148 NP_001157620 transcription factor E2-alpha isoform 2
NM_001164149 NP_001157621 transcription factor E2-alpha isoform 3
NM_001164150 NP_001157622 transcription factor E2-alpha isoform 4
NM_001164151 NP_001157623 transcription factor E2-alpha isoform 5
NM_001164152 NP_001157624 transcription factor E2-alpha isoform 6
NM_001164153 NP_001157625 transcription factor E2-alpha isoform 7
NM_011548 NP_035678 transcription factor E2-alpha isoform 8



Homo sapiens (human) TCF3 NP_001129611.1
Pan troglodytes (chimpanzee) TCF3 XP_003954574.1
Canis lupus familiaris (dog) TCF3 XP_542197.3
Bos taurus (cattle) TCF3 NP_001179627.1
Mus musculus (house mouse) Tcf3 NP_001157619.1
Rattus norvegicus (Norway rat) Tcf3 NP_598208.2
Gallus gallus (chicken) TCF3 NP_989817.2
Danio rerio (zebrafish) tcf3b NP_001166154.1
Xenopus (Silurana) tropicalis (western clawed frog) tcf3 NP_001093743.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Tcf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tcf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu19356 NM_001164147 Mus musculus transcription factor 3 (Tcf3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OMu19727
NM_001164148 Mus musculus transcription factor 3 (Tcf3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OMu19548 NM_001164149 Mus musculus transcription factor 3 (Tcf3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $384.30
$549.00
OMu19304 NM_001164150 Mus musculus transcription factor 3 (Tcf3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OMu19666 NM_001164151 Mus musculus transcription factor 3 (Tcf3), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OMu19320 NM_001164152 Mus musculus transcription factor 3 (Tcf3), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $384.30
$549.00
OMu19599 NM_001164153 Mus musculus transcription factor 3 (Tcf3), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $384.30
$549.00
OMu19377 NM_011548 Mus musculus transcription factor 3 (Tcf3), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $384.30
$549.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu19356
Clone ID Related Accession (Same CDS sequence) NM_001164147
Accession Version NM_001164147.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1962bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK146786.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCCCAGAC GAGGACGAGG ACGACCTTCT CCCCCCAGAG
CAGAAGGCGG AGCGGGAGAA GGAGCGCCGG GTGGCCAATA ATGCCCGAGA GCGCCTGCGG
GTCCGCGACA TCAATGAGGC CTTTAAGGAG CTCGGCCGCA TGTGCCAGCT GCACCTCAGC
AGCGAGAAGC CGCAGACCAA ACTGCTCATC CTGCACCAGG CCGTGGCCGT CATCCTCAGC
CTGGAGCAGC AGGTGCGAGA ACGCAACCTG AACCCCAAAG CAGCCTGCTT GAAGCGGAGG
GAGGAGGAGA AGGTGTCTGG CGTGGTCGGG GACCCACAGC TGGCCCTGTC AGCCGCCCAC
CCGGGCCTGG GTGAGGCCCA CAACCCAGCC GGGCACCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157619.1
CDS392..2353
Misc Feature(1)344..346(+)
Misc Feature(2)2027..2209(+)
Misc Feature(3)2036..2152(+)
Misc Feature(4)2060..2062(+)
Misc Feature(5)2078..2206(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1714
Gene:Tcf3
Gene Synonym:
Exon (16)1715..1841
Gene:Tcf3
Gene Synonym:
Exon (17)1842..1974
Gene:Tcf3
Gene Synonym:
Exon (18)1975..2210
Gene:Tcf3
Gene Synonym:
Exon (19)2211..3320
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164147
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164147

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCCCAGAC GAGGACGAGG ACGACCTTCT CCCCCCAGAG
CAGAAGGCGG AGCGGGAGAA GGAGCGCCGG GTGGCCAATA ATGCCCGAGA GCGCCTGCGG
GTCCGCGACA TCAATGAGGC CTTTAAGGAG CTCGGCCGCA TGTGCCAGCT GCACCTCAGC
AGCGAGAAGC CGCAGACCAA ACTGCTCATC CTGCACCAGG CCGTGGCCGT CATCCTCAGC
CTGGAGCAGC AGGTGCGAGA ACGCAACCTG AACCCCAAAG CAGCCTGCTT GAAGCGGAGG
GAGGAGGAGA AGGTGTCTGG CGTGGTCGGG GACCCACAGC TGGCCCTGTC AGCCGCCCAC
CCGGGCCTGG GTGAGGCCCA CAACCCAGCC GGGCACCTGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19727
Clone ID Related Accession (Same CDS sequence) NM_001164148
Accession Version NM_001164148.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (2) has an alternate splice site in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC018260.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CCCAGACGAG GACGAGGACG ACCTTCTCCC CCCAGAGCAG
AAGGCGGAGC GGGAGAAGGA GCGCCGGGTG GCCAATAATG CCCGAGAGCG CCTGCGGGTC
CGCGACATCA ATGAGGCCTT TAAGGAGCTC GGCCGCATGT GCCAGCTGCA CCTCAGCAGC
GAGAAGCCGC AGACCAAACT GCTCATCCTG CACCAGGCCG TGGCCGTCAT CCTCAGCCTG
GAGCAGCAGG TGCGAGAACG CAACCTGAAC CCCAAAGCAG CCTGCTTGAA GCGGAGGGAG
GAGGAGAAGG TGTCTGGCGT GGTCGGGGAC CCACAGCTGG CCCTGTCAGC CGCCCACCCG
GGCCTGGGTG AGGCCCACAA CCCAGCCGGG CACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157620.1
CDS392..2350
Misc Feature(1)344..346(+)
Misc Feature(2)1553..1660(+)
Misc Feature(3)2024..2206(+)
Misc Feature(4)2033..2149(+)
Misc Feature(5)2057..2059(+)
Misc Feature(6)2075..2203(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2207
Gene:Tcf3
Gene Synonym:
Exon (19)2208..3317
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164148
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164148

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CCCAGACGAG GACGAGGACG ACCTTCTCCC CCCAGAGCAG
AAGGCGGAGC GGGAGAAGGA GCGCCGGGTG GCCAATAATG CCCGAGAGCG CCTGCGGGTC
CGCGACATCA ATGAGGCCTT TAAGGAGCTC GGCCGCATGT GCCAGCTGCA CCTCAGCAGC
GAGAAGCCGC AGACCAAACT GCTCATCCTG CACCAGGCCG TGGCCGTCAT CCTCAGCCTG
GAGCAGCAGG TGCGAGAACG CAACCTGAAC CCCAAAGCAG CCTGCTTGAA GCGGAGGGAG
GAGGAGAAGG TGTCTGGCGT GGTCGGGGAC CCACAGCTGG CCCTGTCAGC CGCCCACCCG
GGCCTGGGTG AGGCCCACAA CCCAGCCGGG CACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19548
Clone ID Related Accession (Same CDS sequence) NM_001164149
Accession Version NM_001164149.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1956bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (3) has two alternate splice sites in the coding region, compared to variant 1. The resulting isoform (3) lacks two internal aa, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK156265.1, AK017617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCCC AGACGAGGAC GAGGACGACC TTCTCCCCCC AGAGCAGAAG
GCGGAGCGGG AGAAGGAGCG CCGGGTGGCC AATAATGCCC GAGAGCGCCT GCGGGTCCGC
GACATCAATG AGGCCTTTAA GGAGCTCGGC CGCATGTGCC AGCTGCACCT CAGCAGCGAG
AAGCCGCAGA CCAAACTGCT CATCCTGCAC CAGGCCGTGG CCGTCATCCT CAGCCTGGAG
CAGCAGGTGC GAGAACGCAA CCTGAACCCC AAAGCAGCCT GCTTGAAGCG GAGGGAGGAG
GAGAAGGTGT CTGGCGTGGT CGGGGACCCA CAGCTGGCCC TGTCAGCCGC CCACCCGGGC
CTGGGTGAGG CCCACAACCC AGCCGGGCAC CTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157621.1
CDS392..2347
Misc Feature(1)344..346(+)
Misc Feature(2)1550..1657(+)
Misc Feature(3)2021..2203(+)
Misc Feature(4)2030..2146(+)
Misc Feature(5)2054..2056(+)
Misc Feature(6)2072..2200(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1708
Gene:Tcf3
Gene Synonym:
Exon (16)1709..1835
Gene:Tcf3
Gene Synonym:
Exon (17)1836..1968
Gene:Tcf3
Gene Synonym:
Exon (18)1969..2204
Gene:Tcf3
Gene Synonym:
Exon (19)2205..3314
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164149
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164149

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCCC AGACGAGGAC GAGGACGACC TTCTCCCCCC AGAGCAGAAG
GCGGAGCGGG AGAAGGAGCG CCGGGTGGCC AATAATGCCC GAGAGCGCCT GCGGGTCCGC
GACATCAATG AGGCCTTTAA GGAGCTCGGC CGCATGTGCC AGCTGCACCT CAGCAGCGAG
AAGCCGCAGA CCAAACTGCT CATCCTGCAC CAGGCCGTGG CCGTCATCCT CAGCCTGGAG
CAGCAGGTGC GAGAACGCAA CCTGAACCCC AAAGCAGCCT GCTTGAAGCG GAGGGAGGAG
GAGAAGGTGT CTGGCGTGGT CGGGGACCCA CAGCTGGCCC TGTCAGCCGC CCACCCGGGC
CTGGGTGAGG CCCACAACCC AGCCGGGCAC CTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19304
Clone ID Related Accession (Same CDS sequence) NM_001164150
Accession Version NM_001164150.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1953bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 4
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (4) has an alternate penultimate coding exon, compared to variant 1. The resulting isoform (4) has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK161009.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCAGTACA GATGAGGTGC TGTCCCTGGA GGAGAAGGAC
CTGAGGGACC GGGAGAGGCG TATGGCCAAT AACGCTCGGG AGCGGGTGCG CGTGCGGGAC
ATTAACGAGG CCTTCCGGGA GCTGGGCCGC ATGTGCCAGC TGCACCTCAA GTCGGATAAG
GCGCAGACCA AGCTGCTCAT CCTGCAGCAG GCGGTGCAGG TCATCCTGGG CCTGGAGCAG
CAGGTGCGAG AACGCAACCT GAACCCCAAA GCAGCCTGCT TGAAGCGGAG GGAGGAGGAG
AAGGTGTCTG GCGTGGTCGG GGACCCACAG CTGGCCCTGT CAGCCGCCCA CCCGGGCCTG
GGTGAGGCCC ACAACCCAGC CGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157622.1
CDS392..2344
Misc Feature(1)344..346(+)
Misc Feature(2)2018..2200(+)
Misc Feature(3)2027..2143(+)
Misc Feature(4)2051..2053(+)
Misc Feature(5)2069..2197(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1714
Gene:Tcf3
Gene Synonym:
Exon (16)1715..1841
Gene:Tcf3
Gene Synonym:
Exon (17)1842..1974
Gene:Tcf3
Gene Synonym:
Exon (18)1975..2201
Gene:Tcf3
Gene Synonym:
Exon (19)2202..3311
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164150
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164150

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGCAGAGC AAGATGGAGG ACCGCTTGGA CGAGGCCATC
CATGTCCTGC GAAGCCACGC TGTTGGCACC GCTAGCGATC TCCATGGGCT TTTGCCTGGC
CATGGCGCAC TGACCACGAG CTTCACCGGC CCCATGTCAC TGGGCGGGCG GCATGCCGGC
CTGGTCGGGG GAAGCCATCC TGAGGAGGGC CTCACAAGTG GGGCCAGTCT TTTGCATAAC
CATGCCAGCC TCCCCAGCCA GCCCAGTTCC CTCCCTGACC TCTCACAGAG ACCTCCCGAC
TCCTATAGTG GACTCGGGAG GGCAGGCACA ACAGCGGGTG CCAGCGAGAT CAAGCGGGAG
GAGAAAGAGG ATGAGGAAAT CGCATCAGTA GCCGACGCCG AAGAGGACAA GAAGGACCTG
AAGGTCCCAC GCACGCGCAC CAGCAGTACA GATGAGGTGC TGTCCCTGGA GGAGAAGGAC
CTGAGGGACC GGGAGAGGCG TATGGCCAAT AACGCTCGGG AGCGGGTGCG CGTGCGGGAC
ATTAACGAGG CCTTCCGGGA GCTGGGCCGC ATGTGCCAGC TGCACCTCAA GTCGGATAAG
GCGCAGACCA AGCTGCTCAT CCTGCAGCAG GCGGTGCAGG TCATCCTGGG CCTGGAGCAG
CAGGTGCGAG AACGCAACCT GAACCCCAAA GCAGCCTGCT TGAAGCGGAG GGAGGAGGAG
AAGGTGTCTG GCGTGGTCGG GGACCCACAG CTGGCCCTGT CAGCCGCCCA CCCGGGCCTG
GGTGAGGCCC ACAACCCAGC CGGGCACCTG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19666
Clone ID Related Accession (Same CDS sequence) NM_001164151
Accession Version NM_001164151.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1950bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 5
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (5) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (5) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK038738.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157623.1
CDS392..2341
Misc Feature(1)344..346(+)
Misc Feature(2)2015..2197(+)
Misc Feature(3)2024..2140(+)
Misc Feature(4)2048..2050(+)
Misc Feature(5)2066..2194(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2198
Gene:Tcf3
Gene Synonym:
Exon (19)2199..3308
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164151
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164151

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19320
Clone ID Related Accession (Same CDS sequence) NM_001164152
Accession Version NM_001164152.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1950bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 6
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (6) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (6) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157624.1
CDS392..2341
Misc Feature(1)344..346(+)
Misc Feature(2)2015..2197(+)
Misc Feature(3)2024..2140(+)
Misc Feature(4)2048..2050(+)
Misc Feature(5)2066..2194(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..946
Gene:Tcf3
Gene Synonym:
Exon (9)947..1049
Gene:Tcf3
Gene Synonym:
Exon (10)1050..1207
Gene:Tcf3
Gene Synonym:
Exon (11)1208..1343
Gene:Tcf3
Gene Synonym:
Exon (12)1344..1402
Gene:Tcf3
Gene Synonym:
Exon (13)1403..1481
Gene:Tcf3
Gene Synonym:
Exon (14)1482..1555
Gene:Tcf3
Gene Synonym:
Exon (15)1556..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2198
Gene:Tcf3
Gene Synonym:
Exon (19)2199..3308
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164152
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164152

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGCAGATACT CAGCCGAAGA AGGTCCGGAA GGTTCCGCCT
GGTCTCCCTT CCTCGGTGTA TCCGCCCAGC TCAGGTGACA GCTACAGCAG GGATGCTGCA
GCCTACCCCT CCGCCAAGAC CCCCAGCAGC GCTTACCCCT CCCCCTTCTA CGTGGCAGAT
GGCAGCCTGC ACCCATCAGC TGAGCTCTGG AGTACGCCTA GCCAGGTGGG CTTTGGGCCC
ATGCTAGGTG ACGGCTCTTC CCCTCTGCCC CTTGCACCGG GCAGCAGCTC CGTGGGCAGT
GGTACCTTTG GGGGCCTCCA GCAGCAGGAT CGCATGGGCT ACCAGCTGCA TGGATCTGAG
GTTAATGGCT CGCTCCCAGC TGTATCCAGC TTTTCGGCTG CCCCTGGCAC TTACAGTGGG
ACTTCCGGCC ACACGCCCCC TGTGAGTGGG GCCGCAGCTG AAAGCCTCCT AGGCACCCGA
GGGACTACAG CCAGCAGCTC AGGGGATGCC CTTGGGAAGG CACTGGCCTC GATCTACTCC
CCGGATCACT CCAGCAATAA TTTCTCACCT AGCCCCTCAA CGCCTGTGGG TTCACCCCAG
GGCCTGCCAG GGACATCACA GTGGCCCCGG GCAGGAGCGC CCAGTGCCTT ATCCCCCAAC
TACGATGCAG GTCTCCATGG CCTGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG CAGTACAGAT GAGGTGCTGT CCCTGGAGGA GAAGGACCTG
AGGGACCGGG AGAGGCGTAT GGCCAATAAC GCTCGGGAGC GGGTGCGCGT GCGGGACATT
AACGAGGCCT TCCGGGAGCT GGGCCGCATG TGCCAGCTGC ACCTCAAGTC GGATAAGGCG
CAGACCAAGC TGCTCATCCT GCAGCAGGCG GTGCAGGTCA TCCTGGGCCT GGAGCAGCAG
GTGCGAGAAC GCAACCTGAA CCCCAAAGCA GCCTGCTTGA AGCGGAGGGA GGAGGAGAAG
GTGTCTGGCG TGGTCGGGGA CCCACAGCTG GCCCTGTCAG CCGCCCACCC GGGCCTGGGT
GAGGCCCACA ACCCAGCCGG GCACCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19599
Clone ID Related Accession (Same CDS sequence) NM_001164153
Accession Version NM_001164153.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 7
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. Transcript Variant: This variant (7) has an alternate splice site and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (7) lacks an internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK034777.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157625.1
CDS392..2338
Misc Feature(1)344..346(+)
Misc Feature(2)2012..2194(+)
Misc Feature(3)2021..2137(+)
Misc Feature(4)2045..2047(+)
Misc Feature(5)2063..2191(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1711
Gene:Tcf3
Gene Synonym:
Exon (16)1712..1838
Gene:Tcf3
Gene Synonym:
Exon (17)1839..1971
Gene:Tcf3
Gene Synonym:
Exon (18)1972..2195
Gene:Tcf3
Gene Synonym:
Exon (19)2196..3305
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001164153
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164153

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GCAGAGCAAG ATGGAGGACC GCTTGGACGA GGCCATCCAT
GTCCTGCGAA GCCACGCTGT TGGCACCGCT AGCGATCTCC ATGGGCTTTT GCCTGGCCAT
GGCGCACTGA CCACGAGCTT CACCGGCCCC ATGTCACTGG GCGGGCGGCA TGCCGGCCTG
GTCGGGGGAA GCCATCCTGA GGAGGGCCTC ACAAGTGGGG CCAGTCTTTT GCATAACCAT
GCCAGCCTCC CCAGCCAGCC CAGTTCCCTC CCTGACCTCT CACAGAGACC TCCCGACTCC
TATAGTGGAC TCGGGAGGGC AGGCACAACA GCGGGTGCCA GCGAGATCAA GCGGGAGGAG
AAAGAGGATG AGGAAATCGC ATCAGTAGCC GACGCCGAAG AGGACAAGAA GGACCTGAAG
GTCCCACGCA CGCGCACCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu19377
Clone ID Related Accession (Same CDS sequence) NM_011548
Accession Version NM_011548.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product transcription factor E2-alpha isoform 8
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC152062.6. On Aug 13, 2009 this sequence version replaced gi:46402510. Transcript Variant: This variant (8) has two alternate splice sites and an alternate penultimate coding exon, compared to variant 1. The resulting isoform (8) lacks two internal aa and has a distinct segment in the C-terminal region, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006860.1, AF352579.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_035678.3
CDS392..2338
Misc Feature(1)344..346(+)
Misc Feature(2)2012..2194(+)
Misc Feature(3)2021..2137(+)
Misc Feature(4)2045..2047(+)
Misc Feature(5)2063..2191(+)
Exon (1)1..334
Gene:Tcf3
Gene Synonym:
Exon (2)335..466
Gene:Tcf3
Gene Synonym:
Exon (3)467..539
Gene:Tcf3
Gene Synonym:
Exon (4)540..613
Gene:Tcf3
Gene Synonym:
Exon (5)614..692
Gene:Tcf3
Gene Synonym:
Exon (6)693..760
Gene:Tcf3
Gene Synonym:
Exon (7)761..893
Gene:Tcf3
Gene Synonym:
Exon (8)894..943
Gene:Tcf3
Gene Synonym:
Exon (9)944..1046
Gene:Tcf3
Gene Synonym:
Exon (10)1047..1204
Gene:Tcf3
Gene Synonym:
Exon (11)1205..1340
Gene:Tcf3
Gene Synonym:
Exon (12)1341..1399
Gene:Tcf3
Gene Synonym:
Exon (13)1400..1478
Gene:Tcf3
Gene Synonym:
Exon (14)1479..1552
Gene:Tcf3
Gene Synonym:
Exon (15)1553..1708
Gene:Tcf3
Gene Synonym:
Exon (16)1709..1835
Gene:Tcf3
Gene Synonym:
Exon (17)1836..1968
Gene:Tcf3
Gene Synonym:
Exon (18)1969..2195
Gene:Tcf3
Gene Synonym:
Exon (19)2196..3305
Gene:Tcf3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_011548
Organism Mus musculus (house mouse)
Definition Mus musculus transcription factor 3 (Tcf3), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_011548

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGATGAACC AGTCTCAGAG AATGGCACCC GTGGGCTCTG ACAAGGAACT GAGTGACCTC 
CTGGACTTCA GCATGATGTT CCCGCTACCT GTGGCCAATG GGAAGAGCCG GCCCGCCTCC
CTCGGGGGAA CCCAGTTTGC AGGCTCAGGA CTGGAGGACC GACCCAGCTC AGGCTCCTGG
GGCAGCAGTG ACCAGAACAG TTCTTCCTTT GACCCTAGCC GGACATACAG CGAAGGTGCC
CACTTCAGTG ACTCCCACAG CAGCCTGCCG CCTTCCACGT TCCTAGGAGC TGGGCTTGGA
GGCAAGGGCA GTGAGCGGAA TGCCTATGCC ACCTTTGGGA GAGACACCAG TGTTGGCACC
TTGAGTCAGG CTGGCTTCCT GCCAGGTGAG CTGAGCCTCA GCAGTCCCGG GCCACTGTCC
CCATCGGGCA TCAAGAGCAG CTCCCAGTAT TACCCCTCAT TCCCCAGCAA CCCTCGTCGG
AGAGCTGCAG ATGGTGGCCT GGATACTCAG CCGAAGAAGG TCCGGAAGGT TCCGCCTGGT
CTCCCTTCCT CGGTGTATCC GCCCAGCTCA GGTGACAGCT ACAGCAGGGA TGCTGCAGCC
TACCCCTCCG CCAAGACCCC CAGCAGCGCT TACCCCTCCC CCTTCTACGT GGCAGATGGC
AGCCTGCACC CATCAGCTGA GCTCTGGAGT ACGCCTAGCC AGGTGGGCTT TGGGCCCATG
CTAGGTGACG GCTCTTCCCC TCTGCCCCTT GCACCGGGCA GCAGCTCCGT GGGCAGTGGT
ACCTTTGGGG GCCTCCAGCA GCAGGATCGC ATGGGCTACC AGCTGCATGG ATCTGAGGTT
AATGGCTCGC TCCCAGCTGT ATCCAGCTTT TCGGCTGCCC CTGGCACTTA CAGTGGGACT
TCCGGCCACA CGCCCCCTGT GAGTGGGGCC GCAGCTGAAA GCCTCCTAGG CACCCGAGGG
ACTACAGCCA GCAGCTCAGG GGATGCCCTT GGGAAGGCAC TGGCCTCGAT CTACTCCCCG
GATCACTCCA GCAATAATTT CTCACCTAGC CCCTCAACGC CTGTGGGTTC ACCCCAGGGC
CTGCCAGGGA CATCACAGTG GCCCCGGGCA GGAGCGCCCA GTGCCTTATC CCCCAACTAC
GATGCAGGTC TCCATGGCCT GAGCAAGATG GAGGACCGCT TGGACGAGGC CATCCATGTC
CTGCGAAGCC ACGCTGTTGG CACCGCTAGC GATCTCCATG GGCTTTTGCC TGGCCATGGC
GCACTGACCA CGAGCTTCAC CGGCCCCATG TCACTGGGCG GGCGGCATGC CGGCCTGGTC
GGGGGAAGCC ATCCTGAGGA GGGCCTCACA AGTGGGGCCA GTCTTTTGCA TAACCATGCC
AGCCTCCCCA GCCAGCCCAG TTCCCTCCCT GACCTCTCAC AGAGACCTCC CGACTCCTAT
AGTGGACTCG GGAGGGCAGG CACAACAGCG GGTGCCAGCG AGATCAAGCG GGAGGAGAAA
GAGGATGAGG AAATCGCATC AGTAGCCGAC GCCGAAGAGG ACAAGAAGGA CCTGAAGGTC
CCACGCACGC GCACCAGCAG TACAGATGAG GTGCTGTCCC TGGAGGAGAA GGACCTGAGG
GACCGGGAGA GGCGTATGGC CAATAACGCT CGGGAGCGGG TGCGCGTGCG GGACATTAAC
GAGGCCTTCC GGGAGCTGGG CCGCATGTGC CAGCTGCACC TCAAGTCGGA TAAGGCGCAG
ACCAAGCTGC TCATCCTGCA GCAGGCGGTG CAGGTCATCC TGGGCCTGGA GCAGCAGGTG
CGAGAACGCA ACCTGAACCC CAAAGCAGCC TGCTTGAAGC GGAGGGAGGA GGAGAAGGTG
TCTGGCGTGG TCGGGGACCC ACAGCTGGCC CTGTCAGCCG CCCACCCGGG CCTGGGTGAG
GCCCACAACC CAGCCGGGCA CCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex
J. Immunol. 192 (5), 2460-2470 (2014)
Hauser J, Grundstrom C and Grundstrom T.


book

Id3 and Id2 act as a dual safety mechanism in regulating the development and population size of innate-like gammadelta T cells
J. Immunol. 192 (3), 1055-1063 (2014)
Zhang B, Lin YY, Dai M and Zhuang Y.


book

Cell-intrinsic in vivo requirement for the E47-p21 pathway in long-term hematopoietic stem cells
J. Immunol. 192 (1), 160-168 (2014)
Santos PM, Ding Y and Borghesi L.


book

Combined deletion of Id2 and Id3 genes reveals multiple roles for E proteins in invariant NKT cell development and expansion
J. Immunol. 191 (10), 5052-5064 (2013)
Li J, Wu D, Jiang N and Zhuang Y.


book

Early B-cell factor 1 regulates the expansion of B-cell progenitors in a dose-dependent manner
J. Biol. Chem. 288 (46), 33449-33461 (2013)
Ahsberg J, Ungerback J, Strid T, Welinder E, Stjernberg J, Larsson M, Qian H and Sigvardsson M.


book

Helix-loop-helix transcription factors E12 and E47 are not essential for skeletal or cardiac myogenesis, erythropoiesis, chondrogenesis, or neurogenesis
Proc. Natl. Acad. Sci. U.S.A. 89 (24), 12132-12136 (1992)
Zhuang Y, Kim CG, Bartelmez S, Cheng P, Groudine M and Weintraub H.


book

Murine helix-loop-helix transcriptional activator proteins binding to the E-box motif of the Akv murine leukemia virus enhancer identified by cDNA cloning
Mol. Cell. Biol. 12 (8), 3449-3459 (1992)
Nielsen AL, Pallisgaard N, Pedersen FS and Jorgensen P.


book

A congenic line of the BALB/c mouse strain with the endogenous mouse mammary tumor virus proviral gene Mtv-3: tissue-specific expression and correlation with resistance to mouse mammary tumor virus infection and tumorigenesis
Cancer Res. 50 (12), 3754-3760 (1990)
Hainaut P, Castellazzi M, Gonzales D, Clausse N, Hilgers J and Crepin M.


book

A cDNA from a mouse pancreatic beta cell encoding a putative transcription factor of the insulin gene
Nucleic Acids Res. 18 (5), 1159-1166 (1990)
Walker MD, Park CW, Rosen A and Aronheim A.


book

Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence
Cell 58 (3), 537-544 (1989)
Murre C, McCaw PS, Vaessin H, Caudy M, Jan LY, Jan YN, Cabrera CV, Buskin JN, Hauschka SD and Lassar AB.


 
*
*
*
*