Email to GenScript

Tpm1 tropomyosin 1, alpha [Mus musculus (house mouse)]

Gene Symbol Tpm1
Entrez Gene ID 22003
Full Name tropomyosin 1, alpha
Synonyms AA986836, AI854628, TM2, Tm3, Tmpa, Tpm-1, alpha-TM
General protein information
Preferred Names
tropomyosin alpha-1 chain
Names
tropomyosin alpha-1 chain
tropomyosin-1
alpha tropomyosin
alpha-tropomyosin
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

9

9 C|9 36.27 cM

Summary lac of sum
Disorder MIM:

Disorder Html:





Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (Floxed/Frt) (1) 
  • Targeted (knock-out) (2)  1 citation

The following Tpm1 gene sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpm1 gene which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu42703 XM_006511039 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42704 XM_006511040 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu68947 XM_011242714 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu68948 XM_011242715 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42706 XM_006511042 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42705 XM_006511041 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42707 XM_006511043 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42708 XM_006511044 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42709 XM_006511045 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42710 XM_006511046 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42713 XM_006511049 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu68949 XM_011242716 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42714 XM_006511050 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu42715 XM_006511051 PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199
OMu09705 NM_001164253 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09702 NM_001164252 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu08178 NM_024427 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09699 NM_001164251 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09712 NM_001164256 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09697 NM_001164250 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09707 NM_001164254 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09709 NM_001164255 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09693 NM_001164248 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269
OMu09694 NM_001164249 Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $269

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OMu42703
Accession Version XM_006511039.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568961214. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511102.1
CDS210..1073
Misc Feature(1)228..668(+)
Misc Feature(2)351..986(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511039
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511039

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42704
Accession Version XM_006511040.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568961216. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511103.1
CDS270..1133
Misc Feature(1)288..728(+)
Misc Feature(2)411..1046(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511040
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511040

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu68947
Accession Version XM_011242714.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011241016.1
CDS210..1073
Misc Feature(1)228..668(+)
Misc Feature(2)351..986(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_011242714
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011242714

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu68948
Accession Version XM_011242715.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011241017.1
CDS270..1133
Misc Feature(1)288..728(+)
Misc Feature(2)411..1046(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_011242715
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011242715

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATAAGTTT
CTTTGCTTCA CTCCTCCCAA GACTCCTTCG TCAAGCTGGA TGTCCCACCT CTCTGAGCTC
TGCATCTGTC TGCTCTCCAG CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42706
Accession Version XM_006511042.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568961220. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511105.1
CDS210..1064
Misc Feature(1)228..668(+)
Misc Feature(2)351..1061(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511042
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511042

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42705
Accession Version XM_006511041.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TGTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511104.1
CDS210..1064
Misc Feature(1)228..668(+)
Misc Feature(2)351..1061(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511041
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511041

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42707
Accession Version XM_006511043.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568961222. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511106.1
CDS210..1064
Misc Feature(1)228..668(+)
Misc Feature(2)351..1061(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511043
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511043

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42708
Accession Version XM_006511044.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511107.1
CDS210..1064
Misc Feature(1)228..668(+)
Misc Feature(2)351..1061(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511044
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511044

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42709
Accession Version XM_006511045.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCATCAAC TCGAGCAAAA CCGCCGCCTA ACTAACGAGC TAAAGCTGGC CCTGAATGAG
GATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511108.1
CDS210..1055
Misc Feature(1)228..668(+)
Misc Feature(2)351..1049(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511045
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511045

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCATCAAC TCGAGCAAAA CCGCCGCCTA ACTAACGAGC TAAAGCTGGC CCTGAATGAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42710
Accession Version XM_006511046.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCATCAAC TCGAGCAAAA CCGCCGCCTA ACTAACGAGC TAAAGCTGGC CCTGAATGAG
GATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511109.1
CDS210..1055
Misc Feature(1)228..668(+)
Misc Feature(2)351..1049(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511046
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511046

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCATCAAC TCGAGCAAAA CCGCCGCCTA ACTAACGAGC TAAAGCTGGC CCTGAATGAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42713
Accession Version XM_006511049.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 756bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568961234. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTCCTCCC AAGACTCCTT CGTCAAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATCTG TCTGCTCTCC AGCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511112.1
CDS265..1020
Misc Feature(1)298..933(+)
Misc Feature(2)298..615(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511049
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511049

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTCCTCCC AAGACTCCTT CGTCAAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATCTG TCTGCTCTCC AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu68949
Accession Version XM_011242716.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 756bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTCCTCCC AAGACTCCTT CGTCAAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATCTG TCTGCTCTCC AGCTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_011241018.1
CDS265..1020
Misc Feature(1)298..933(+)
Misc Feature(2)298..615(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_011242716
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011242716

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATAAGT TTCTTTGCTT CACTCCTCCC AAGACTCCTT CGTCAAGCTG GATGTCCCAC
CTCTCTGAGC TCTGCATCTG TCTGCTCTCC AGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42714
Accession Version XM_006511050.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X13
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTATGCTCA GAAACTGAAG TACAAGGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511113.1
CDS265..1011
Misc Feature(1)298..1008(+)
Misc Feature(2)298..615(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511050
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511050

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTATGCTCA GAAACTGAAG TACAAGGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu42715
Accession Version XM_006511051.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform X14
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039474.8) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568961238. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCATCA ACTCGAGCAA AACCGCCGCC TAACTAACGA GCTAAAGCTG
GCCCTGAATG AGGATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqXP_006511114.1
CDS265..1002
Misc Feature(1)298..996(+)
Misc Feature(2)298..615(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006511051
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006511051

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCATCA ACTCGAGCAA AACCGCCGCC TAACTAACGA GCTAAAGCTG
GCCCTGAATG AGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09705
Accession Version NM_001164253.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.9cy
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.9, also known as variant 7) differs in the 5' UTR and coding sequence, lacks an alternate internal exon, and contains another alternate internal exon compared to variant Tpm1.6. The encoded isoform (Tpm1.9cy) has an alternate N-terminus, has a short internal region of sequence differences and is shorter, compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU515442.1, CA978948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCCCATGC CAAAGAAGAA AACCTTAGTA TGCACCAGAT GCTGGATCAG
ACTTTACTGG AGCTAAACAA CATGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157725.1
CDS163..909
Misc Feature(1)112..114(+)
Misc Feature(2)196..906(+)
Misc Feature(3)196..513(+)
Exon (1)1..294
Gene:Tpm1
Gene Synonym:
Exon (2)295..428
Gene:Tpm1
Gene Synonym:
Exon (3)429..546
Gene:Tpm1
Gene Synonym:
Exon (4)547..617
Gene:Tpm1
Gene Synonym:
Exon (5)618..693
Gene:Tpm1
Gene Synonym:
Exon (6)694..756
Gene:Tpm1
Gene Synonym:
Exon (7)757..826
Gene:Tpm1
Gene Synonym:
Exon (8)827..1675
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, t dbSNP:224815593
111 111 -, cag dbSNP:221916303
114 114 -, gag dbSNP:233391734
142 142 a, t dbSNP:13468991
160 160 a, g dbSNP:260544136
342 342 a, g dbSNP:250806073
573 573 c, t dbSNP:13468990
939 939 a, g dbSNP:13468988
1010 1010 -, a dbSNP:229700548
1048 1048 g, t dbSNP:242689381
1049 1049 a, t dbSNP:222738780
1067 1067 a, g dbSNP:4227736
1081 1081 -, gcgcagaaaggc dbSNP:259157761
1163 1163 c, t dbSNP:50364447
1195 1195 c, t dbSNP:49342505
1196 1196 a, g dbSNP:4227735
1211 1211 a, t dbSNP:4227734
1237 1237 a, g dbSNP:4227733
1326 1326 a, t dbSNP:4227732
1400 1400 c, t dbSNP:4227731
1475 1475 c, t dbSNP:4227730
1496 1496 c, t dbSNP:4227729
1504 1504 c, t dbSNP:4227728
1514 1514 c, t dbSNP:13468986
1521 1521 a, g dbSNP:4227727
1528 1528 -, c dbSNP:387011431
1536 1536 -, c dbSNP:216175731
1552 1552 a, g dbSNP:29885390
1578 1578 c, t dbSNP:13468987

Target ORF information:

RefSeq Version NM_001164253
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164253

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCCAAG TTCGACAGCT GGAAGAACAG
TTAAGAATAA TGGATCAGAC CTTGAAAGCA TTAATGGCTG CAGAGGATAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCCCATGC CAAAGAAGAA AACCTTAGTA TGCACCAGAT GCTGGATCAG
ACTTTACTGG AGCTAAACAA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09702
Accession Version NM_001164252.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.8cy
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.8, also known as variant 6) differs in the 5' UTR and coding sequence compared to variant Tpm1.6. The encoded isoform (Tpm1.8cy) has an alternate N-terminus compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX218332.1, CF904726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCCCATGC CAAAGAAGAA AACCTTAGTA TGCACCAGAT GCTGGATCAG
ACTTTACTGG AGCTAAACAA CATGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157724.1
CDS163..909
Misc Feature(1)112..114(+)
Misc Feature(2)196..906(+)
Misc Feature(3)196..513(+)
Exon (1)1..294
Gene:Tpm1
Gene Synonym:
Exon (2)295..428
Gene:Tpm1
Gene Synonym:
Exon (3)429..546
Gene:Tpm1
Gene Synonym:
Exon (4)547..617
Gene:Tpm1
Gene Synonym:
Exon (5)618..693
Gene:Tpm1
Gene Synonym:
Exon (6)694..756
Gene:Tpm1
Gene Synonym:
Exon (7)757..826
Gene:Tpm1
Gene Synonym:
Exon (8)827..1675
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, t dbSNP:224815593
111 111 -, cag dbSNP:221916303
114 114 -, gag dbSNP:233391734
142 142 a, t dbSNP:13468991
160 160 a, g dbSNP:260544136
342 342 a, g dbSNP:250806073
573 573 c, t dbSNP:13468990
939 939 a, g dbSNP:13468988
1010 1010 -, a dbSNP:229700548
1048 1048 g, t dbSNP:242689381
1049 1049 a, t dbSNP:222738780
1067 1067 a, g dbSNP:4227736
1081 1081 -, gcgcagaaaggc dbSNP:259157761
1163 1163 c, t dbSNP:50364447
1195 1195 c, t dbSNP:49342505
1196 1196 a, g dbSNP:4227735
1211 1211 a, t dbSNP:4227734
1237 1237 a, g dbSNP:4227733
1326 1326 a, t dbSNP:4227732
1400 1400 c, t dbSNP:4227731
1475 1475 c, t dbSNP:4227730
1496 1496 c, t dbSNP:4227729
1504 1504 c, t dbSNP:4227728
1514 1514 c, t dbSNP:13468986
1521 1521 a, g dbSNP:4227727
1528 1528 -, c dbSNP:387011431
1536 1536 -, c dbSNP:216175731
1552 1552 a, g dbSNP:29885390
1578 1578 c, t dbSNP:13468987

Target ORF information:

RefSeq Version NM_001164252
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164252

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGAGAAAG TGGCCCATGC CAAAGAAGAA AACCTTAGTA TGCACCAGAT GCTGGATCAG
ACTTTACTGG AGCTAAACAA CATGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08178
Accession Version NM_024427.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.6cy
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. On May 1, 2007 this sequence version replaced gi:142361543. Transcript Variant: This variant (Tpm1.6, also known as variant 3) represents one of the longest transcripts and encodes one of the longest isoforms (Tpm1.6cy). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK003175.1, BC026720.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_077745.2
CDS82..936
Misc Feature(1)58..60(+)
Misc Feature(2)100..540(+)
Misc Feature(3)223..933(+)
Exon (1)1..195
Gene:Tpm1
Gene Synonym:
Exon (2)196..321
Gene:Tpm1
Gene Synonym:
Exon (3)322..455
Gene:Tpm1
Gene Synonym:
Exon (4)456..573
Gene:Tpm1
Gene Synonym:
Exon (5)574..644
Gene:Tpm1
Gene Synonym:
Exon (6)645..720
Gene:Tpm1
Gene Synonym:
Exon (7)721..783
Gene:Tpm1
Gene Synonym:
Exon (8)784..853
Gene:Tpm1
Gene Synonym:
Exon (9)854..1702
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
70 70 a, g dbSNP:262585965
165 165 a, g dbSNP:13468989
369 369 a, g dbSNP:250806073
600 600 c, t dbSNP:13468990
966 966 a, g dbSNP:13468988
1037 1037 -, a dbSNP:229700548
1075 1075 g, t dbSNP:242689381
1076 1076 a, t dbSNP:222738780
1094 1094 a, g dbSNP:4227736
1108 1108 -, gcgcagaaaggc dbSNP:259157761
1190 1190 c, t dbSNP:50364447
1222 1222 c, t dbSNP:49342505
1223 1223 a, g dbSNP:4227735
1238 1238 a, t dbSNP:4227734
1264 1264 a, g dbSNP:4227733
1353 1353 a, t dbSNP:4227732
1427 1427 c, t dbSNP:4227731
1502 1502 c, t dbSNP:4227730
1523 1523 c, t dbSNP:4227729
1531 1531 c, t dbSNP:4227728
1541 1541 c, t dbSNP:13468986
1548 1548 a, g dbSNP:4227727
1555 1555 -, c dbSNP:387011431
1563 1563 -, c dbSNP:216175731
1579 1579 a, g dbSNP:29885390
1605 1605 c, t dbSNP:13468987

Target ORF information:

RefSeq Version NM_024427
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_024427

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09699
Accession Version NM_001164251.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.5cy
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.5, also known as variant 5) differs in the 3' UTR and coding sequence, lacks internal exon, and contains another alternate internal exon compared to variant Tpm1.6. The encoded isoform (Tpm1.5cy) is the same length but has short regions of sequence differences, compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077713.1, BU515546.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157723.1
CDS82..936
Misc Feature(1)58..60(+)
Misc Feature(2)100..540(+)
Misc Feature(3)223..933(+)
Exon (1)1..195
Gene:Tpm1
Gene Synonym:
Exon (2)196..321
Gene:Tpm1
Gene Synonym:
Exon (3)322..455
Gene:Tpm1
Gene Synonym:
Exon (4)456..573
Gene:Tpm1
Gene Synonym:
Exon (5)574..644
Gene:Tpm1
Gene Synonym:
Exon (6)645..720
Gene:Tpm1
Gene Synonym:
Exon (7)721..783
Gene:Tpm1
Gene Synonym:
Exon (8)784..853
Gene:Tpm1
Gene Synonym:
Exon (9)854..932
Gene:Tpm1
Gene Synonym:
Exon (10)933..1210
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
70 70 a, g dbSNP:262585965
165 165 a, g dbSNP:13468989
369 369 a, g dbSNP:250806073
600 600 c, t dbSNP:13468990
937 937 c, g dbSNP:30287498
970 970 a, g dbSNP:51303329
1071 1071 c, g dbSNP:221980396
1122 1122 c, t dbSNP:257589584
1178 1178 a, g dbSNP:50259137

Target ORF information:

RefSeq Version NM_001164251
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164251

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09712
Accession Version NM_001164256.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 738bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.12br
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.12, also known as variant 10) differs in the 5' and 3' UTRs as well as the 5' and 3' coding sequences compared to variant Tpm1.6. The resulting isoform (Tpm1.12br) has shorter and distinct N- and C-termini compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU560031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCATCA ACTCGAGCAA AACCGCCGCC TAACTAACGA GCTAAAGCTG
GCCCTGAATG AGGATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157728.1
CDS163..900
Misc Feature(1)112..114(+)
Misc Feature(2)196..894(+)
Misc Feature(3)196..513(+)
Exon (1)1..294
Gene:Tpm1
Gene Synonym:
Exon (2)295..428
Gene:Tpm1
Gene Synonym:
Exon (3)429..546
Gene:Tpm1
Gene Synonym:
Exon (4)547..617
Gene:Tpm1
Gene Synonym:
Exon (5)618..693
Gene:Tpm1
Gene Synonym:
Exon (6)694..756
Gene:Tpm1
Gene Synonym:
Exon (7)757..826
Gene:Tpm1
Gene Synonym:
Exon (8)827..1550
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, t dbSNP:224815593
111 111 -, cag dbSNP:221916303
114 114 -, gag dbSNP:233391734
142 142 a, t dbSNP:13468991
160 160 a, g dbSNP:260544136
342 342 a, g dbSNP:250806073
573 573 c, t dbSNP:13468990
843 843 a, g dbSNP:249323604
864 864 a, c dbSNP:229574168
896 896 a, g dbSNP:260969086
909 909 c, g dbSNP:243104155
932 932 a, g dbSNP:33676839
956 956 a, g dbSNP:259565097
1008 1008 g, t dbSNP:245720013
1143 1143 a, g dbSNP:223086620
1180 1180 -, tccc dbSNP:262374282
1180 1180 -, tc dbSNP:218219770
1180 1180 c, t dbSNP:260790087
1180 1180 -, t dbSNP:257601625
1188 1188 c, g dbSNP:30385254
1219 1219 c, t dbSNP:226030561
1264 1264 a, c dbSNP:30422741
1531 1531 a, c dbSNP:232807330

Target ORF information:

RefSeq Version NM_001164256
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164256

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGATCAAC TCTACCATCA ACTCGAGCAA AACCGCCGCC TAACTAACGA GCTAAAGCTG
GCCCTGAATG AGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09697
Accession Version NM_001164250.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.7cy
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.7, also known as variant 4) lacks an exon and contains another alternate exon compared to variant Tpm1.6. The encoded isoform (Tpm1.7cy) is the same length but has short regions of sequence differences and an alternate C-terminus, compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU513182.1, BQ900500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157722.1
CDS82..936
Misc Feature(1)58..60(+)
Misc Feature(2)100..540(+)
Misc Feature(3)223..933(+)
Exon (1)1..195
Gene:Tpm1
Gene Synonym:
Exon (2)196..321
Gene:Tpm1
Gene Synonym:
Exon (3)322..455
Gene:Tpm1
Gene Synonym:
Exon (4)456..573
Gene:Tpm1
Gene Synonym:
Exon (5)574..644
Gene:Tpm1
Gene Synonym:
Exon (6)645..720
Gene:Tpm1
Gene Synonym:
Exon (7)721..783
Gene:Tpm1
Gene Synonym:
Exon (8)784..853
Gene:Tpm1
Gene Synonym:
Exon (9)854..1702
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
70 70 a, g dbSNP:262585965
165 165 a, g dbSNP:13468989
369 369 a, g dbSNP:250806073
600 600 c, t dbSNP:13468990
966 966 a, g dbSNP:13468988
1037 1037 -, a dbSNP:229700548
1075 1075 g, t dbSNP:242689381
1076 1076 a, t dbSNP:222738780
1094 1094 a, g dbSNP:4227736
1108 1108 -, gcgcagaaaggc dbSNP:259157761
1190 1190 c, t dbSNP:50364447
1222 1222 c, t dbSNP:49342505
1223 1223 a, g dbSNP:4227735
1238 1238 a, t dbSNP:4227734
1264 1264 a, g dbSNP:4227733
1353 1353 a, t dbSNP:4227732
1427 1427 c, t dbSNP:4227731
1502 1502 c, t dbSNP:4227730
1523 1523 c, t dbSNP:4227729
1531 1531 c, t dbSNP:4227728
1541 1541 c, t dbSNP:13468986
1548 1548 a, g dbSNP:4227727
1555 1555 -, c dbSNP:387011431
1563 1563 -, c dbSNP:216175731
1579 1579 a, g dbSNP:29885390
1605 1605 c, t dbSNP:13468987

Target ORF information:

RefSeq Version NM_001164250
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164250

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCCAAGTT CGACAGCTGG AAGAACAGTT AAGAATAATG
GATCAGACCT TGAAAGCATT AATGGCTGCA GAGGATAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09707
Accession Version NM_001164254.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 747bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.13
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.13, also known as variant 8) differs in the 5' and 3' UTRs as well as the 5' and 3' coding sequences compared to variant Tpm1.6. The encoded isoform (Tpm1.13) has alternate N- and C- termini and is shorter, compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CX241654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTATGCTCA GAAACTGAAG TACAAGGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157726.1
CDS163..909
Misc Feature(1)112..114(+)
Misc Feature(2)196..906(+)
Misc Feature(3)196..513(+)
Exon (1)1..294
Gene:Tpm1
Gene Synonym:
Exon (2)295..428
Gene:Tpm1
Gene Synonym:
Exon (3)429..546
Gene:Tpm1
Gene Synonym:
Exon (4)547..617
Gene:Tpm1
Gene Synonym:
Exon (5)618..693
Gene:Tpm1
Gene Synonym:
Exon (6)694..756
Gene:Tpm1
Gene Synonym:
Exon (7)757..826
Gene:Tpm1
Gene Synonym:
Exon (8)827..905
Gene:Tpm1
Gene Synonym:
Exon (9)906..1183
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
54 54 c, t dbSNP:224815593
111 111 -, cag dbSNP:221916303
114 114 -, gag dbSNP:233391734
142 142 a, t dbSNP:13468991
160 160 a, g dbSNP:260544136
342 342 a, g dbSNP:250806073
573 573 c, t dbSNP:13468990
910 910 c, g dbSNP:30287498
943 943 a, g dbSNP:51303329
1044 1044 c, g dbSNP:221980396
1095 1095 c, t dbSNP:257589584
1151 1151 a, g dbSNP:50259137

Target ORF information:

RefSeq Version NM_001164254
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164254

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCGGGTA GCAGCTCGCT GGAGGCGGTG CGGAGAAAGA TCCGGAGCCT GCAGGAGCAG 
GCGGATGCCG CGGAGGAGCG TGCGGGCAGC CTGCAGCGCG AGCTGGACCA GGAGCGGAAG
CTGCGGGAAA CCGCTGAAGC TGACGTAGCT TCTCTGAACA GACGCATCCA GCTGGTTGAG
GAGGAGTTGG ATCGTGCTCA GGAGCGTCTG GCCACAGCTC TGCAGAAGCT GGAGGAGGCC
GAGAAGGCTG CAGATGAGAG TGAGAGAGGC ATGAAAGTCA TTGAAAGCCG AGCCCAAAAA
GATGAAGAAA AGATGGAGAT TCAGGAGATC CAGCTGAAAG AGGCCAAGCA CATTGCTGAA
GATGCTGACC GGAAGTATGA AGAGGTGGCC CGTAAGCTGG TCATCATCGA GAGCGACCTG
GAACGTGCAG AGGAGCGGGC TGAGCTCTCA GAAGGCAAAT GTGCCGAGCT TGAAGAAGAA
TTGAAAACGG TGACGAACAA CTTGAAGTCA CTGGAGGCTC AGGCTGAGAA GTACTCTCAG
AAGGAAGACA AATATGAAGA GGAGATCAAG GTTCTCTCTG ACAAGCTGAA GGAGGCTGAA
ACTCGGGCTG AGTTTGCAGA GAGATCAGTA ACCAAATTGG AGAAAAGCAT TGATGACTTA
GAAGACGAGC TGTATGCTCA GAAACTGAAG TACAAGGCCA TCAGCGAGGA GCTGGACCAC
GCTCTCAACG ATATGACTTC CATATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09709
Accession Version NM_001164255.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 846bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.10br
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.10, also known as variant 9) differs in the 3' UTR and coding sequence compared to variant Tpm1.6. The resulting isoform (9) has a shorter and distinct C-terminus compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCATCAAC TCGAGCAAAA CCGCCGCCTA ACTAACGAGC TAAAGCTGGC CCTGAATGAG
GATTAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157727.1
CDS82..927
Misc Feature(1)58..60(+)
Misc Feature(2)100..540(+)
Misc Feature(3)223..921(+)
Exon (1)1..195
Gene:Tpm1
Gene Synonym:
Exon (2)196..321
Gene:Tpm1
Gene Synonym:
Exon (3)322..455
Gene:Tpm1
Gene Synonym:
Exon (4)456..573
Gene:Tpm1
Gene Synonym:
Exon (5)574..644
Gene:Tpm1
Gene Synonym:
Exon (6)645..720
Gene:Tpm1
Gene Synonym:
Exon (7)721..783
Gene:Tpm1
Gene Synonym:
Exon (8)784..853
Gene:Tpm1
Gene Synonym:
Exon (9)854..1577
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
70 70 a, g dbSNP:262585965
165 165 a, g dbSNP:13468989
369 369 a, g dbSNP:250806073
600 600 c, t dbSNP:13468990
870 870 a, g dbSNP:249323604
891 891 a, c dbSNP:229574168
923 923 a, g dbSNP:260969086
936 936 c, g dbSNP:243104155
959 959 a, g dbSNP:33676839
983 983 a, g dbSNP:259565097
1035 1035 g, t dbSNP:245720013
1170 1170 a, g dbSNP:223086620
1207 1207 -, tccc dbSNP:262374282
1207 1207 -, tc dbSNP:218219770
1207 1207 c, t dbSNP:260790087
1207 1207 -, t dbSNP:257601625
1215 1215 c, g dbSNP:30385254
1246 1246 c, t dbSNP:226030561
1291 1291 a, c dbSNP:30422741
1558 1558 a, c dbSNP:232807330

Target ORF information:

RefSeq Version NM_001164255
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164255

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGATCAACTC
TACCATCAAC TCGAGCAAAA CCGCCGCCTA ACTAACGAGC TAAAGCTGGC CCTGAATGAG
GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09693
Accession Version NM_001164248.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.1st
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.1, also known as variant 1) differs in the 3' UTR and coding sequence compared to variant Tpm1.6. The encoded isoform (Tpm1.1st) is the same length, but has an alternate C-terminus, compared to isoform Tpm1.6cy. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169188.1, AK168783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157720.1
CDS82..936
Misc Feature(1)58..60(+)
Misc Feature(2)82..84(+)
Misc Feature(3)100..540(+)
Misc Feature(4)223..933(+)
Misc Feature(5)238..240(+)
Misc Feature(6)262..264(+)
Misc Feature(7)316..318(+)
Misc Feature(8)403..405(+)
Misc Feature(9)601..603(+)
Misc Feature(10)697..699(+)
Misc Feature(11)925..927(+)
Misc Feature(12)928..930(+)
Exon (1)1..195
Gene:Tpm1
Gene Synonym:
Exon (2)196..321
Gene:Tpm1
Gene Synonym:
Exon (3)322..455
Gene:Tpm1
Gene Synonym:
Exon (4)456..573
Gene:Tpm1
Gene Synonym:
Exon (5)574..644
Gene:Tpm1
Gene Synonym:
Exon (6)645..720
Gene:Tpm1
Gene Synonym:
Exon (7)721..783
Gene:Tpm1
Gene Synonym:
Exon (8)784..853
Gene:Tpm1
Gene Synonym:
Exon (9)854..932
Gene:Tpm1
Gene Synonym:
Exon (10)933..1210
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
70 70 a, g dbSNP:262585965
165 165 a, g dbSNP:13468989
369 369 a, g dbSNP:250806073
600 600 c, t dbSNP:13468990
937 937 c, g dbSNP:30287498
970 970 a, g dbSNP:51303329
1071 1071 c, g dbSNP:221980396
1122 1122 c, t dbSNP:257589584
1178 1178 a, g dbSNP:50259137

Target ORF information:

RefSeq Version NM_001164248
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164248

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTGGAA
GATGAGCTGG TGTCACTGCA AAAGAAACTC AAGGGCACTG AAGATGAACT GGACAAATAC
TCCGAGGCTC TCAAAGATGC CCAGGAGAAA CTGGAGCTGG CGGAGAAAAA GGCCACAGAT
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGACGAGCTG
TATGCTCAGA AACTGAAGTA CAAGGCCATC AGCGAGGAGC TGGACCACGC TCTCAACGAT
ATGACTTCCA TATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu09694
Accession Version NM_001164249.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 855bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-JUN-2015
Organism Mus musculus (house mouse)
Product tropomyosin alpha-1 chain isoform Tpm1.2st
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166370.3. Transcript Variant: This variant (Tpm1.2, also known as variant 2) lacks an in-frame exon and has an alternate in-frame exon compared to variant Tpm1.6. The encoded isoform (Tpm1.2st) is the same length but has a short region of sequence differences, compared to isoform Tpm1.6cy. Sequence Note: The genomic coordinates were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM461207.1, BC145307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeqNP_001157721.1
CDS82..936
Misc Feature(1)58..60(+)
Misc Feature(2)100..540(+)
Misc Feature(3)223..933(+)
Exon (1)1..195
Gene:Tpm1
Gene Synonym:
Exon (2)196..321
Gene:Tpm1
Gene Synonym:
Exon (3)322..455
Gene:Tpm1
Gene Synonym:
Exon (4)456..573
Gene:Tpm1
Gene Synonym:
Exon (5)574..644
Gene:Tpm1
Gene Synonym:
Exon (6)645..720
Gene:Tpm1
Gene Synonym:
Exon (7)721..783
Gene:Tpm1
Gene Synonym:
Exon (8)784..853
Gene:Tpm1
Gene Synonym:
Exon (9)854..1702
Gene:Tpm1
Gene Synonym:
Translation
Position Chain Variation Link
70 70 a, g dbSNP:262585965
165 165 a, g dbSNP:13468989
369 369 a, g dbSNP:250806073
600 600 c, t dbSNP:13468990
966 966 a, g dbSNP:13468988
1037 1037 -, a dbSNP:229700548
1075 1075 g, t dbSNP:242689381
1076 1076 a, t dbSNP:222738780
1094 1094 a, g dbSNP:4227736
1108 1108 -, gcgcagaaaggc dbSNP:259157761
1190 1190 c, t dbSNP:50364447
1222 1222 c, t dbSNP:49342505
1223 1223 a, g dbSNP:4227735
1238 1238 a, t dbSNP:4227734
1264 1264 a, g dbSNP:4227733
1353 1353 a, t dbSNP:4227732
1427 1427 c, t dbSNP:4227731
1502 1502 c, t dbSNP:4227730
1523 1523 c, t dbSNP:4227729
1531 1531 c, t dbSNP:4227728
1541 1541 c, t dbSNP:13468986
1548 1548 a, g dbSNP:4227727
1555 1555 -, c dbSNP:387011431
1563 1563 -, c dbSNP:216175731
1579 1579 a, g dbSNP:29885390
1605 1605 c, t dbSNP:13468987

Target ORF information:

RefSeq Version NM_001164249
Organism Mus musculus (house mouse)
Definition Mus musculus tropomyosin 1, alpha (Tpm1), transcript variant Tpm1.2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001164249

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGACGCCA TCAAGAAGAA GATGCAGATG CTGAAGCTCG ACAAAGAGAA CGCCTTGGAT 
CGAGCTGAGC AAGCGGAGGC TGATAAGAAG GCGGCGGAAG ACCGGAGCAA GCAGCTCGAG
GAGGACATCG CAGCGAAAGA GAAGCTGCTG CGGGCGTCGG AGGACGAGCG GGACCGGGTG
CTAGAGGAGC TGCACAAGGC GGAGGACAGC CTCCTGGCTG CCGACGAGAC CGCCGCCAAG
GCTGAAGCTG ACGTAGCTTC TCTGAACAGA CGCATCCAGC TGGTTGAGGA GGAGTTGGAT
CGTGCTCAGG AGCGTCTGGC CACAGCTCTG CAGAAGCTGG AGGAGGCCGA GAAGGCTGCA
GATGAGAGTG AGAGAGGCAT GAAAGTCATT GAAAGCCGAG CCCAAAAAGA TGAAGAAAAG
ATGGAGATTC AGGAGATCCA GCTGAAAGAG GCCAAGCACA TTGCTGAAGA TGCTGACCGG
AAGTATGAAG AGGTGGCCCG TAAGCTGGTC ATCATCGAGA GCGACCTGGA ACGTGCAGAG
GAGCGGGCTG AGCTCTCAGA AGGCAAATGT GCCGAGCTTG AAGAAGAATT GAAAACGGTG
ACGAACAACT TGAAGTCACT GGAGGCTCAG GCTGAGAAGT ACTCTCAGAA GGAAGACAAA
TATGAAGAGG AGATCAAGGT TCTCTCTGAC AAGCTGAAGG AGGCTGAAAC TCGGGCTGAG
TTTGCAGAGA GATCAGTAAC CAAATTGGAG AAAAGCATTG ATGACTTAGA AGAGAAAGTG
GCCCATGCCA AAGAAGAAAA CCTTAGTATG CACCAGATGC TGGATCAGAC TTTACTGGAG
CTAAACAACA TGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.