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Ebi3 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Ebi3
Entrez Gene ID 50498
Full Name Epstein-Barr virus induced gene 3
Synonyms EBI-3, IL-27
General protein information
Preferred Names
interleukin-27 subunit beta
Names
interleukin-27 subunit beta
IL-27B
IL-27 subunit beta
epstein-Barr virus-induced gene 3 protein homolog
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

17

17 D|17 29.08 cM

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
XM_006524599 XP_006524662 interleukin-27 subunit beta isoform X1
NM_015766 NP_056581 interleukin-27 subunit beta precursor


Homo sapiens (human) EBI3 NP_005746.2
Pan troglodytes (chimpanzee) EBI3 XP_001138182.1
Canis lupus familiaris (dog) EBI3 XP_542161.3
Bos taurus (cattle) EBI3 NP_001093835.1
Mus musculus (house mouse) Ebi3 NP_056581.1
Rattus norvegicus (Norway rat) Ebi3 NP_001102891.1
Danio rerio (zebrafish) ebi3 NP_001106980.1
Xenopus (Silurana) tropicalis (western clawed frog) ebi3 XP_002934057.1

Component

ID Name Evidence
GO:0005576 extracellular region IEA
GO:0005615 extracellular space IEA
GO:0016020 membrane IEA

Function

ID Name Evidence
GO:0004896 cytokine receptor activity ISO
GO:0005125 cytokine activity IEA
GO:0005515 protein binding IPI
GO:0045523 interleukin-27 receptor binding IDA

Process

ID Name Evidence
GO:0019221 cytokine-mediated signaling pathway ISO

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

The following Ebi3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ebi3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu53816 XM_006524599 PREDICTED: Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu22389
NM_015766 Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
Starting from $99.50
$199.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OMu53816
Accession Version XM_006524599.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product interleukin-27 subunit beta isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039649.8) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:569000954. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGCTAAATG TCACTGCAGT GCACCCAGGC GGCGCCAGCA GCAGCCTCCT AGCCTTTGTG 
GCTGAGCGAA TCATCAAGCC GGACCCTCCG GAAGGCGTGC GCCTGCGCAC AGCGGGACAG
CGCCTGCAGG TGCTCTGGCA TCCCCCTGCT TCCTGGCCCT TCCCGGACAT CTTCTCTCTC
AAGTACCGAC TCCGCTACCG GCGCCGAGGA GCCTCTCACT TCCGCCAGGT GGGACCCATT
GAAGCCACGA CTTTCACCCT CAGGAACTCG AAACCCCATG CCAAGTATTG CATCCAGGTG
TCAGCTCAGG ACCTCACAGA TTATGGGAAA CCAAGTGACT GGAGCCTCCC TGGGCAAGTA
GAAAGTGCAC CCCATAAGCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006524662.1
CDS410..793
Misc Feature(1)488..754(+)
Misc Feature(2)488..730(+)
Misc Feature(3)731..745(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006524599
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006524599

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCTAAATG TCACTGCAGT GCACCCAGGC GGCGCCAGCA GCAGCCTCCT AGCCTTTGTG 
GCTGAGCGAA TCATCAAGCC GGACCCTCCG GAAGGCGTGC GCCTGCGCAC AGCGGGACAG
CGCCTGCAGG TGCTCTGGCA TCCCCCTGCT TCCTGGCCCT TCCCGGACAT CTTCTCTCTC
AAGTACCGAC TCCGCTACCG GCGCCGAGGA GCCTCTCACT TCCGCCAGGT GGGACCCATT
GAAGCCACGA CTTTCACCCT CAGGAACTCG AAACCCCATG CCAAGTATTG CATCCAGGTG
TCAGCTCAGG ACCTCACAGA TTATGGGAAA CCAAGTGACT GGAGCCTCCC TGGGCAAGTA
GAAAGTGCAC CCCATAAGCC CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22389
Accession Version NM_015766.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 687bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product interleukin-27 subunit beta precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY134465.1, CX240802.1, BI134394.1 and AW495532.1. On Jul 15, 2005 this sequence version replaced gi:7657051. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC008209.1, AF013114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCCAAGC TGCTCTTCCT GTCACTTGCC CTCTGGGCCA GCCGCTCCCC TGGTTACACT 
GAAACAGCTC TCGTGGCTCT AAGCCAGCCC AGAGTGCAAT GCCATGCTTC TCGGTATCCC
GTGGCCGTGG ACTGCTCCTG GACTCCTCTC CAGGCTCCCA ACTCCACCAG ATCCACGTCC
TTCATTGCCA CTTACAGGCT CGGTGTGGCC ACCCAGCAGC AGAGCCAGCC CTGCCTACAA
CGGAGCCCCC AGGCCTCCCG ATGCACCATC CCCGACGTGC ACCTGTTCTC CACGGTGCCC
TACATGCTAA ATGTCACTGC AGTGCACCCA GGCGGCGCCA GCAGCAGCCT CCTAGCCTTT
GTGGCTGAGC GAATCATCAA GCCGGACCCT CCGGAAGGCG TGCGCCTGCG CACAGCGGGA
CAGCGCCTGC AGGTGCTCTG GCATCCCCCT GCTTCCTGGC CCTTCCCGGA CATCTTCTCT
CTCAAGTACC GACTCCGCTA CCGGCGCCGA GGAGCCTCTC ACTTCCGCCA GGTGGGACCC
ATTGAAGCCA CGACTTTCAC CCTCAGGAAC TCGAAACCCC ATGCCAAGTA TTGCATCCAG
GTGTCAGCTC AGGACCTCAC AGATTATGGG AAACCAAGTG ACTGGAGCCT CCCTGGGCAA
GTAGAAAGTG CACCCCATAA GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_056581.1
CDS240..926
Misc Feature(1)621..887(+)
Misc Feature(2)621..863(+)
Misc Feature(3)864..878(+)
Exon (1)1..306
Gene:Ebi3
Gene Synonym:
Exon (2)307..436
Gene:Ebi3
Gene Synonym:
Exon (3)437..615
Gene:Ebi3
Gene Synonym:
Exon (4)616..770
Gene:Ebi3
Gene Synonym:
Exon (5)771..1171
Gene:Ebi3
Gene Synonym:
Translation
Position Chain Variation Link
63 63 a, g dbSNP:251628645
172 172 a, g dbSNP:213797717
315 315 g, t dbSNP:48036461
447 447 g, t dbSNP:248769211
464 464 c, t dbSNP:263101489
543 543 a, c dbSNP:49084831
758 758 c, t dbSNP:47326715
797 797 c, t dbSNP:249255852
928 928 a, g dbSNP:263263697
932 932 a, g dbSNP:226757620
981 981 a, g dbSNP:239159120
1057 1057 c, t dbSNP:257009969
1149 1149 a, g dbSNP:48509128

Target ORF information:

RefSeq Version NM_015766
Organism Mus musculus (house mouse)
Definition Mus musculus Epstein-Barr virus induced gene 3 (Ebi3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015766

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGTCCAAGC TGCTCTTCCT GTCACTTGCC CTCTGGGCCA GCCGCTCCCC TGGTTACACT 
GAAACAGCTC TCGTGGCTCT AAGCCAGCCC AGAGTGCAAT GCCATGCTTC TCGGTATCCC
GTGGCCGTGG ACTGCTCCTG GACTCCTCTC CAGGCTCCCA ACTCCACCAG ATCCACGTCC
TTCATTGCCA CTTACAGGCT CGGTGTGGCC ACCCAGCAGC AGAGCCAGCC CTGCCTACAA
CGGAGCCCCC AGGCCTCCCG ATGCACCATC CCCGACGTGC ACCTGTTCTC CACGGTGCCC
TACATGCTAA ATGTCACTGC AGTGCACCCA GGCGGCGCCA GCAGCAGCCT CCTAGCCTTT
GTGGCTGAGC GAATCATCAA GCCGGACCCT CCGGAAGGCG TGCGCCTGCG CACAGCGGGA
CAGCGCCTGC AGGTGCTCTG GCATCCCCCT GCTTCCTGGC CCTTCCCGGA CATCTTCTCT
CTCAAGTACC GACTCCGCTA CCGGCGCCGA GGAGCCTCTC ACTTCCGCCA GGTGGGACCC
ATTGAAGCCA CGACTTTCAC CCTCAGGAAC TCGAAACCCC ATGCCAAGTA TTGCATCCAG
GTGTCAGCTC AGGACCTCAC AGATTATGGG AAACCAAGTG ACTGGAGCCT CCCTGGGCAA
GTAGAAAGTG CACCCCATAA GCCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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