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Myl9 cDNA ORF clone, Mus musculus (house mouse)

Gene Symbol Myl9
Entrez Gene ID 98932
Full Name myosin, light polypeptide 9, regulatory
Synonyms AI327049, MLC20, Mylc2c, RLC-C
General protein information
Preferred Names
myosin regulatory light polypeptide 9
Names
myosin regulatory light polypeptide 9
myosin regulatory light chain 9
myosin light chain, regulatory C
myosin regulatory light chain 2, smooth muscle isoform
Gene Type protein-coding
Organism Mus musculus (house mouse)
Genome

2

2|2 H1

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
XM_006500453 XP_006500516 myosin regulatory light polypeptide 9 isoform X1
NM_172118 NP_742116 myosin regulatory light polypeptide 9

KEGG
mmu04270 Vascular smooth muscle contraction
mmu04510 Focal adhesion
mmu04530 Tight junction
mmu04810 Regulation of actin cytoskeleton
mmu04670 Leukocyte transendothelial migration
mmu04921 Oxytocin signaling pathway
mmu04022 cGMP-PKG signaling pathway
mmu04024 cAMP signaling pathway
WikiPathways
WP216 Striated Muscle Contraction


Homo sapiens (human) MYL9 NP_006088.2
Pan troglodytes (chimpanzee) MYL9 XP_514618.3
Canis lupus familiaris (dog) MYL9 NP_001239095.1
Bos taurus (cattle) MYL9 NP_001068702.1
Mus musculus (house mouse) Myl9 NP_742116.1
Rattus norvegicus (Norway rat) Myl9 NP_001094355.1
Gallus gallus (chicken) MYL9 NP_990609.1
Danio rerio (zebrafish) myl9b NP_998377.1
Xenopus (Silurana) tropicalis (western clawed frog) myl9 NP_001096250.1

Component

ID Name Evidence
GO:0001725 stress fiber IDA
GO:0016459 myosin complex IEA
GO:0016460 myosin II complex IDA
GO:0030018 Z disc IDA

Function

ID Name Evidence
GO:0003774 motor activity IEA
GO:0005509 calcium ion binding IEA

Process

ID Name Evidence
GO:0006936 muscle contraction ISO

The following Myl9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myl9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OMu03192
XM_006500453 PREDICTED: Mus musculus myosin, light polypeptide 9, regulatory (Myl9), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00
OMu03192
NM_172118 Mus musculus myosin, light polypeptide 9, regulatory (Myl9), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OMu03192
Accession Version XM_006500453.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 09-FEB-2015
Organism Mus musculus (house mouse)
Product myosin regulatory light polypeptide 9 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_039207.8) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced gi:568918976. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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481
ATGTCGAGCA AGAGAGCCAA GGCCAAGACC ACCAAGAAGA GGCCCCAGAG GGCTACGTCC 
AATGTCTTCG CCATGTTTGA CCAGTCCCAG ATCCAGGAGT TTAAGGAGGC CTTCAACATG
ATTGATCAGA ACCGAGATGG CTTCATTGAT AAGGAGGACC TGCACGACAT GCTGGCCTCT
CTGGGGAAGA ACCCCACAGA CGAGTATCTG GAGGGCATGA TGAACGAGGC GCCAGGGCCC
ATCAACTTCA CCATGTTCCT CACAATGTTT GGGGAGAAGC TGAACGGCAC AGACCCCGAG
GATGTGATCC GCAATGCCTT TGCCTGCTTT GATGAGGAGG CCTCAGGCTT CATCCACGAG
GACCACCTGA GGGAGCTGCT CACCACCATG GGCGACCGAT TCACGGATGA GGAGGTGGAC
GAGATGTACC GCGAGGCACC CATTGATAAG AAGGGCAACT TCAACTACGT GGAGTTCACT
CGCATCCTCA AACACGGCGC CAAGGACAAG GACGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006500516.1
CDS131..649
Misc Feature(1)185..637(+)
Misc Feature(2)227..>361(+)
Misc Feature(3)254..289(+)
Misc Feature(4)437..622(+)
Misc Feature(5)461..604(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006500453
Organism Mus musculus (house mouse)
Definition PREDICTED: Mus musculus myosin, light polypeptide 9, regulatory (Myl9), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006500453

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGAGCA AGAGAGCCAA GGCCAAGACC ACCAAGAAGA GGCCCCAGAG GGCTACGTCC 
AATGTCTTCG CCATGTTTGA CCAGTCCCAG ATCCAGGAGT TTAAGGAGGC CTTCAACATG
ATTGATCAGA ACCGAGATGG CTTCATTGAT AAGGAGGACC TGCACGACAT GCTGGCCTCT
CTGGGGAAGA ACCCCACAGA CGAGTATCTG GAGGGCATGA TGAACGAGGC GCCAGGGCCC
ATCAACTTCA CCATGTTCCT CACAATGTTT GGGGAGAAGC TGAACGGCAC AGACCCCGAG
GATGTGATCC GCAATGCCTT TGCCTGCTTT GATGAGGAGG CCTCAGGCTT CATCCACGAG
GACCACCTGA GGGAGCTGCT CACCACCATG GGCGACCGAT TCACGGATGA GGAGGTGGAC
GAGATGTACC GCGAGGCACC CATTGATAAG AAGGGCAACT TCAACTACGT GGAGTTCACT
CGCATCCTCA AACACGGCGC CAAGGACAAG GACGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu03192
Accession Version NM_172118.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-FEB-2015
Organism Mus musculus (house mouse)
Product myosin regulatory light polypeptide 9
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK020258.1. On or before Sep 7, 2008 this sequence version replaced gi:149250365, gi:149250223. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK020258.1, AK159880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
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361
421
481
ATGTCGAGCA AGAGAGCCAA GGCCAAGACC ACCAAGAAGA GGCCCCAGAG GGCTACGTCC 
AATGTCTTCG CCATGTTTGA CCAGTCCCAG ATCCAGGAGT TTAAGGAGGC CTTCAACATG
ATTGATCAGA ACCGAGATGG CTTCATTGAT AAGGAGGACC TGCACGACAT GCTGGCCTCT
CTGGGGAAGA ACCCCACAGA CGAGTATCTG GAGGGCATGA TGAACGAGGC GCCAGGGCCC
ATCAACTTCA CCATGTTCCT CACAATGTTT GGGGAGAAGC TGAACGGCAC AGACCCCGAG
GATGTGATCC GCAATGCCTT TGCCTGCTTT GATGAGGAGG CCTCAGGCTT CATCCACGAG
GACCACCTGA GGGAGCTGCT CACCACCATG GGCGACCGAT TCACGGATGA GGAGGTGGAC
GAGATGTACC GCGAGGCACC CATTGATAAG AAGGGCAACT TCAACTACGT GGAGTTCACT
CGCATCCTCA AACACGGCGC CAAGGACAAG GACGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_742116.1
CDS70..588
Misc Feature(1)124..576(+)
Misc Feature(2)124..126(+)
Misc Feature(3)166..>300(+)
Misc Feature(4)193..228(+)
Misc Feature(5)376..561(+)
Misc Feature(6)400..543(+)
Exon (1)1..43
Gene:Myl9
Gene Synonym:
Exon (2)44..253
Gene:Myl9
Gene Synonym:
Exon (3)254..415
Gene:Myl9
Gene Synonym:
Exon (4)416..1083
Gene:Myl9
Gene Synonym:
Translation
Position Chain Variation Link
12 12 a, g dbSNP:27309463
48 48 a, g dbSNP:253325528
65 65 c, g dbSNP:216867736
204 204 a, c dbSNP:27309443
241 241 c, t dbSNP:27309442
276 276 c, t dbSNP:27309432
333 333 a, t dbSNP:27309431
507 507 a, g dbSNP:263280753
516 516 c, t dbSNP:228251071
522 522 a, g dbSNP:248282142
567 567 c, t dbSNP:261527313
573 573 c, t dbSNP:4223600
592 592 a, g dbSNP:248823238
600 600 c, t dbSNP:212827757
664 664 c, t dbSNP:27309426
706 706 c, t dbSNP:255994977
711 711 a, g dbSNP:215010272
752 752 -, acta dbSNP:221700052
794 794 a, g dbSNP:233432988
883 883 a, c dbSNP:4223599
883 883 -, g dbSNP:258024339
947 947 -, ac dbSNP:248711060
986 986 a, c dbSNP:252548767
1040 1040 a, g dbSNP:217284483
1083 1083 c, t dbSNP:27309425

Target ORF information:

RefSeq Version NM_172118
Organism Mus musculus (house mouse)
Definition Mus musculus myosin, light polypeptide 9, regulatory (Myl9), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_172118

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTCGAGCA AGAGAGCCAA GGCCAAGACC ACCAAGAAGA GGCCCCAGAG GGCTACGTCC 
AATGTCTTCG CCATGTTTGA CCAGTCCCAG ATCCAGGAGT TTAAGGAGGC CTTCAACATG
ATTGATCAGA ACCGAGATGG CTTCATTGAT AAGGAGGACC TGCACGACAT GCTGGCCTCT
CTGGGGAAGA ACCCCACAGA CGAGTATCTG GAGGGCATGA TGAACGAGGC GCCAGGGCCC
ATCAACTTCA CCATGTTCCT CACAATGTTT GGGGAGAAGC TGAACGGCAC AGACCCCGAG
GATGTGATCC GCAATGCCTT TGCCTGCTTT GATGAGGAGG CCTCAGGCTT CATCCACGAG
GACCACCTGA GGGAGCTGCT CACCACCATG GGCGACCGAT TCACGGATGA GGAGGTGGAC
GAGATGTACC GCGAGGCACC CATTGATAAG AAGGGCAACT TCAACTACGT GGAGTTCACT
CGCATCCTCA AACACGGCGC CAAGGACAAG GACGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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