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Klrk1 cDNA ORF clone, Rattus norvegicus (Norway rat)

Gene Symbol Klrk1
Entrez Gene ID 24934
Full Name killer cell lectin-like receptor subfamily K, member 1
Synonyms NKG2D, Nkrp2
General protein information
Preferred Names
NKG2-D type II integral membrane protein
Names
NKG2-D type II integral membrane protein
NKLLR
NK cell receptor D
NK lectin-like receptor
NKG2-D-activating NK receptor
NKR-P2, ortholog of human NKG2D
Gene Type protein-coding
Organism Rattus norvegicus (Norway rat)
Genome

4

4q42

Summary functions as a natural cytotoxicity receptor to stimulate perforin-mediated elimination of ligand-expressing tumor cells [RGD, Feb 2006]. lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
XM_006237073 XP_006237135 NKG2-D type II integral membrane protein isoform X2
XM_006237074 XP_006237136 NKG2-D type II integral membrane protein isoform X3
XM_006237075 XP_006237137 NKG2-D type II integral membrane protein isoform X3
XM_006237076 XP_006237138 NKG2-D type II integral membrane protein isoform X3
XM_006237077 XP_006237139 NKG2-D type II integral membrane protein isoform X1
NM_133512 NP_598196 NKG2-D type II integral membrane protein

KEGG
rno04650 Natural killer cell mediated cytotoxicity
rno05144 Malaria


Pan troglodytes (chimpanzee) KLRK1 NP_001009059.1
Macaca mulatta (Rhesus monkey) NKG2D NP_001028061.1
Canis lupus familiaris (dog) KLRK1 XP_005637217.1
Bos taurus (cattle) KLRK1 NP_001068607.1
Mus musculus (house mouse) Klrk1 NP_149069.1
Rattus norvegicus (Norway rat) Klrk1 NP_598196.1

Component

ID Name Evidence
GO:0009897 external side of plasma membrane IEA
GO:0009897 external side of plasma membrane ISO
GO:0009986 cell surface ISO
GO:0016020 membrane IEA
GO:0016021 integral to membrane IEA

Function

ID Name Evidence
GO:0004872 receptor activity IEA
GO:0005488 binding IEA
GO:0005529 sugar binding IEA
GO:0032394 MHC class Ib receptor activity IEA
GO:0032394 MHC class Ib receptor activity ISO
GO:0042288 MHC class I protein binding IEA
GO:0042288 MHC class I protein binding ISO
GO:0042803 protein homodimerization activity ISO
GO:0045233 natural killer cell receptor activity IDA

Process

ID Name Evidence
GO:0002223 stimulatory C-type lectin receptor signaling pathway IEA
GO:0002223 stimulatory C-type lectin receptor signaling pathway ISO
GO:0030101 natural killer cell activation IEA
GO:0030101 natural killer cell activation ISO
GO:0032729 positive regulation of interferon-gamma production IEA
GO:0032729 positive regulation of interferon-gamma production ISO
GO:0045429 positive regulation of nitric oxide biosynthetic process IEA
GO:0045429 positive regulation of nitric oxide biosynthetic process ISO
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity IEA
GO:0045954 positive regulation of natural killer cell mediated cytotoxicity ISO
GO:0050830 defense response to Gram-positive bacterium IEA
GO:0050830 defense response to Gram-positive bacterium ISO
GO:0071222 cellular response to lipopolysaccharide IEA
GO:0071222 cellular response to lipopolysaccharide ISO

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Klrk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klrk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
ORa27590 XM_006237073 PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
ORa27591 XM_006237074 PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
ORa27591 XM_006237075 PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
ORa27591 XM_006237076 PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
ORa13224 XM_006237077 PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00
ORa13224 NM_133512 Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $199.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee that the protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID ORa27590
Accession Version XM_006237073.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2014
Organism Rattus norvegicus (Norway rat)
Product NKG2-D type II integral membrane protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007905797.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced gi:564348311. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGATGGCAT GGGTTCGTGA TCGAAAGTCT CATCACCCAG AGATGAGCAA ATGCCATAAT 
TATGACCTCA AGCCAGCAAA GTGGGATACT TCTCAAGAGC ATCAGAAACA AAGATCTGCA
CTCCCTACCA GCCGACCTGG AGAGAATGGT ATCATAAGAC GAAGGTCTTC TATAGAAGAA
CTCAAAATAT CTCCACTGTT CGTTGTTCGA GTCCTTGTTG CAGCCATGAC CATTCGCTTC
ACCGTTATCA CATTGACATG GCTTGCTGTT TTCATAACTT TGTTATGCAA CAAGGAAGTT
TCAGTTTCCT CAAGAGAGGG CTACTGTGGC CCATGCCCTA ACGACTGGAT ATGTCACAGA
AACAACTGTT ACCAATTTTT TAATGAGAAC AAAGCCTGGA ACCAGAGCCA AGCTTCCTGT
TTGTCTCAAA ATTCCAGCCT GCTGAAGATA TACAGTAAAG AAGAACAGGA TTTCTTAAAG
CTGGTTAAGT CCTATCACTG GATGGGACTA GTCCAGAGCC CAGCAAATGG CTCCTGGCAG
TGGGAAGATG GCTCCTCTCT ATCACCCAAC GAGTTAACAC TGGTGAAAAC ACCAAGCGGA
ACCTGTGCTG TCTATGGCTC AAGCTTTAAG GCCTACACAG AAGACTGTTC AAATCCAAAC
ACATACATCT GTATGAAAAG GGCAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006237135.1
CDS1234..1923
Misc Feature(1)1408..1914(+)
Misc Feature(2)1567..1911(+)
Misc Feature(3)1726..1872(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006237073
Organism Rattus norvegicus (Norway rat)
Definition PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006237073

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGATGGCAT GGGTTCGTGA TCGAAAGTCT CATCACCCAG AGATGAGCAA ATGCCATAAT 
TATGACCTCA AGCCAGCAAA GTGGGATACT TCTCAAGAGC ATCAGAAACA AAGATCTGCA
CTCCCTACCA GCCGACCTGG AGAGAATGGT ATCATAAGAC GAAGGTCTTC TATAGAAGAA
CTCAAAATAT CTCCACTGTT CGTTGTTCGA GTCCTTGTTG CAGCCATGAC CATTCGCTTC
ACCGTTATCA CATTGACATG GCTTGCTGTT TTCATAACTT TGTTATGCAA CAAGGAAGTT
TCAGTTTCCT CAAGAGAGGG CTACTGTGGC CCATGCCCTA ACGACTGGAT ATGTCACAGA
AACAACTGTT ACCAATTTTT TAATGAGAAC AAAGCCTGGA ACCAGAGCCA AGCTTCCTGT
TTGTCTCAAA ATTCCAGCCT GCTGAAGATA TACAGTAAAG AAGAACAGGA TTTCTTAAAG
CTGGTTAAGT CCTATCACTG GATGGGACTA GTCCAGAGCC CAGCAAATGG CTCCTGGCAG
TGGGAAGATG GCTCCTCTCT ATCACCCAAC GAGTTAACAC TGGTGAAAAC ACCAAGCGGA
ACCTGTGCTG TCTATGGCTC AAGCTTTAAG GCCTACACAG AAGACTGTTC AAATCCAAAC
ACATACATCT GTATGAAAAG GGCAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa27591
Accession Version XM_006237074.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 684bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2014
Organism Rattus norvegicus (Norway rat)
Product NKG2-D type II integral membrane protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007905797.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced gi:564348313. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACTTGT GTCAACTTGA ATTGCTTCCT CCAGAGATGA GCAAATGCCA TAATTATGAC 
CTCAAGCCAG CAAAGTGGGA TACTTCTCAA GAGCATCAGA AACAAAGATC TGCACTCCCT
ACCAGCCGAC CTGGAGAGAA TGGTATCATA AGACGAAGGT CTTCTATAGA AGAACTCAAA
ATATCTCCAC TGTTCGTTGT TCGAGTCCTT GTTGCAGCCA TGACCATTCG CTTCACCGTT
ATCACATTGA CATGGCTTGC TGTTTTCATA ACTTTGTTAT GCAACAAGGA AGTTTCAGTT
TCCTCAAGAG AGGGCTACTG TGGCCCATGC CCTAACGACT GGATATGTCA CAGAAACAAC
TGTTACCAAT TTTTTAATGA GAACAAAGCC TGGAACCAGA GCCAAGCTTC CTGTTTGTCT
CAAAATTCCA GCCTGCTGAA GATATACAGT AAAGAAGAAC AGGATTTCTT AAAGCTGGTT
AAGTCCTATC ACTGGATGGG ACTAGTCCAG AGCCCAGCAA ATGGCTCCTG GCAGTGGGAA
GATGGCTCCT CTCTATCACC CAACGAGTTA ACACTGGTGA AAACACCAAG CGGAACCTGT
GCTGTCTATG GCTCAAGCTT TAAGGCCTAC ACAGAAGACT GTTCAAATCC AAACACATAC
ATCTGTATGA AAAGGGCAGT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006237136.1
CDS139..822
Misc Feature(1)307..813(+)
Misc Feature(2)466..810(+)
Misc Feature(3)625..771(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006237074
Organism Rattus norvegicus (Norway rat)
Definition PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006237074

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACTTGT GTCAACTTGA ATTGCTTCCT CCAGAGATGA GCAAATGCCA TAATTATGAC 
CTCAAGCCAG CAAAGTGGGA TACTTCTCAA GAGCATCAGA AACAAAGATC TGCACTCCCT
ACCAGCCGAC CTGGAGAGAA TGGTATCATA AGACGAAGGT CTTCTATAGA AGAACTCAAA
ATATCTCCAC TGTTCGTTGT TCGAGTCCTT GTTGCAGCCA TGACCATTCG CTTCACCGTT
ATCACATTGA CATGGCTTGC TGTTTTCATA ACTTTGTTAT GCAACAAGGA AGTTTCAGTT
TCCTCAAGAG AGGGCTACTG TGGCCCATGC CCTAACGACT GGATATGTCA CAGAAACAAC
TGTTACCAAT TTTTTAATGA GAACAAAGCC TGGAACCAGA GCCAAGCTTC CTGTTTGTCT
CAAAATTCCA GCCTGCTGAA GATATACAGT AAAGAAGAAC AGGATTTCTT AAAGCTGGTT
AAGTCCTATC ACTGGATGGG ACTAGTCCAG AGCCCAGCAA ATGGCTCCTG GCAGTGGGAA
GATGGCTCCT CTCTATCACC CAACGAGTTA ACACTGGTGA AAACACCAAG CGGAACCTGT
GCTGTCTATG GCTCAAGCTT TAAGGCCTAC ACAGAAGACT GTTCAAATCC AAACACATAC
ATCTGTATGA AAAGGGCAGT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa27591
Accession Version XM_006237075.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 684bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2014
Organism Rattus norvegicus (Norway rat)
Product NKG2-D type II integral membrane protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007905797.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced gi:564348315. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACTTGT GTCAACTTGA ATTGCTTCCT CCAGAGATGA GCAAATGCCA TAATTATGAC 
CTCAAGCCAG CAAAGTGGGA TACTTCTCAA GAGCATCAGA AACAAAGATC TGCACTCCCT
ACCAGCCGAC CTGGAGAGAA TGGTATCATA AGACGAAGGT CTTCTATAGA AGAACTCAAA
ATATCTCCAC TGTTCGTTGT TCGAGTCCTT GTTGCAGCCA TGACCATTCG CTTCACCGTT
ATCACATTGA CATGGCTTGC TGTTTTCATA ACTTTGTTAT GCAACAAGGA AGTTTCAGTT
TCCTCAAGAG AGGGCTACTG TGGCCCATGC CCTAACGACT GGATATGTCA CAGAAACAAC
TGTTACCAAT TTTTTAATGA GAACAAAGCC TGGAACCAGA GCCAAGCTTC CTGTTTGTCT
CAAAATTCCA GCCTGCTGAA GATATACAGT AAAGAAGAAC AGGATTTCTT AAAGCTGGTT
AAGTCCTATC ACTGGATGGG ACTAGTCCAG AGCCCAGCAA ATGGCTCCTG GCAGTGGGAA
GATGGCTCCT CTCTATCACC CAACGAGTTA ACACTGGTGA AAACACCAAG CGGAACCTGT
GCTGTCTATG GCTCAAGCTT TAAGGCCTAC ACAGAAGACT GTTCAAATCC AAACACATAC
ATCTGTATGA AAAGGGCAGT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006237137.1
CDS208..891
Misc Feature(1)376..882(+)
Misc Feature(2)535..879(+)
Misc Feature(3)694..840(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006237075
Organism Rattus norvegicus (Norway rat)
Definition PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006237075

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACTTGT GTCAACTTGA ATTGCTTCCT CCAGAGATGA GCAAATGCCA TAATTATGAC 
CTCAAGCCAG CAAAGTGGGA TACTTCTCAA GAGCATCAGA AACAAAGATC TGCACTCCCT
ACCAGCCGAC CTGGAGAGAA TGGTATCATA AGACGAAGGT CTTCTATAGA AGAACTCAAA
ATATCTCCAC TGTTCGTTGT TCGAGTCCTT GTTGCAGCCA TGACCATTCG CTTCACCGTT
ATCACATTGA CATGGCTTGC TGTTTTCATA ACTTTGTTAT GCAACAAGGA AGTTTCAGTT
TCCTCAAGAG AGGGCTACTG TGGCCCATGC CCTAACGACT GGATATGTCA CAGAAACAAC
TGTTACCAAT TTTTTAATGA GAACAAAGCC TGGAACCAGA GCCAAGCTTC CTGTTTGTCT
CAAAATTCCA GCCTGCTGAA GATATACAGT AAAGAAGAAC AGGATTTCTT AAAGCTGGTT
AAGTCCTATC ACTGGATGGG ACTAGTCCAG AGCCCAGCAA ATGGCTCCTG GCAGTGGGAA
GATGGCTCCT CTCTATCACC CAACGAGTTA ACACTGGTGA AAACACCAAG CGGAACCTGT
GCTGTCTATG GCTCAAGCTT TAAGGCCTAC ACAGAAGACT GTTCAAATCC AAACACATAC
ATCTGTATGA AAAGGGCAGT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa27591
Accession Version XM_006237076.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 684bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2014
Organism Rattus norvegicus (Norway rat)
Product NKG2-D type II integral membrane protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007905797.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced gi:564348317. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACTTGT GTCAACTTGA ATTGCTTCCT CCAGAGATGA GCAAATGCCA TAATTATGAC 
CTCAAGCCAG CAAAGTGGGA TACTTCTCAA GAGCATCAGA AACAAAGATC TGCACTCCCT
ACCAGCCGAC CTGGAGAGAA TGGTATCATA AGACGAAGGT CTTCTATAGA AGAACTCAAA
ATATCTCCAC TGTTCGTTGT TCGAGTCCTT GTTGCAGCCA TGACCATTCG CTTCACCGTT
ATCACATTGA CATGGCTTGC TGTTTTCATA ACTTTGTTAT GCAACAAGGA AGTTTCAGTT
TCCTCAAGAG AGGGCTACTG TGGCCCATGC CCTAACGACT GGATATGTCA CAGAAACAAC
TGTTACCAAT TTTTTAATGA GAACAAAGCC TGGAACCAGA GCCAAGCTTC CTGTTTGTCT
CAAAATTCCA GCCTGCTGAA GATATACAGT AAAGAAGAAC AGGATTTCTT AAAGCTGGTT
AAGTCCTATC ACTGGATGGG ACTAGTCCAG AGCCCAGCAA ATGGCTCCTG GCAGTGGGAA
GATGGCTCCT CTCTATCACC CAACGAGTTA ACACTGGTGA AAACACCAAG CGGAACCTGT
GCTGTCTATG GCTCAAGCTT TAAGGCCTAC ACAGAAGACT GTTCAAATCC AAACACATAC
ATCTGTATGA AAAGGGCAGT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006237138.1
CDS46..729
Misc Feature(1)214..720(+)
Misc Feature(2)373..717(+)
Misc Feature(3)532..678(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006237076
Organism Rattus norvegicus (Norway rat)
Definition PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006237076

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGACTTGT GTCAACTTGA ATTGCTTCCT CCAGAGATGA GCAAATGCCA TAATTATGAC 
CTCAAGCCAG CAAAGTGGGA TACTTCTCAA GAGCATCAGA AACAAAGATC TGCACTCCCT
ACCAGCCGAC CTGGAGAGAA TGGTATCATA AGACGAAGGT CTTCTATAGA AGAACTCAAA
ATATCTCCAC TGTTCGTTGT TCGAGTCCTT GTTGCAGCCA TGACCATTCG CTTCACCGTT
ATCACATTGA CATGGCTTGC TGTTTTCATA ACTTTGTTAT GCAACAAGGA AGTTTCAGTT
TCCTCAAGAG AGGGCTACTG TGGCCCATGC CCTAACGACT GGATATGTCA CAGAAACAAC
TGTTACCAAT TTTTTAATGA GAACAAAGCC TGGAACCAGA GCCAAGCTTC CTGTTTGTCT
CAAAATTCCA GCCTGCTGAA GATATACAGT AAAGAAGAAC AGGATTTCTT AAAGCTGGTT
AAGTCCTATC ACTGGATGGG ACTAGTCCAG AGCCCAGCAA ATGGCTCCTG GCAGTGGGAA
GATGGCTCCT CTCTATCACC CAACGAGTTA ACACTGGTGA AAACACCAAG CGGAACCTGT
GCTGTCTATG GCTCAAGCTT TAAGGCCTAC ACAGAAGACT GTTCAAATCC AAACACATAC
ATCTGTATGA AAAGGGCAGT GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa13224
Accession Version XM_006237077.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 648bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 07-AUG-2014
Organism Rattus norvegicus (Norway rat)
Product NKG2-D type II integral membrane protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_007905797.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced gi:564348319. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGAGCAAAT GCCATAATTA TGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAGCAT 
CAGAAACAAA GATCTGCACT CCCTACCAGC CGACCTGGAG AGAATGGTAT CATAAGACGA
AGGTCTTCTA TAGAAGAACT CAAAATATCT CCACTGTTCG TTGTTCGAGT CCTTGTTGCA
GCCATGACCA TTCGCTTCAC CGTTATCACA TTGACATGGC TTGCTGTTTT CATAACTTTG
TTATGCAACA AGGAAGTTTC AGTTTCCTCA AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC
GACTGGATAT GTCACAGAAA CAACTGTTAC CAATTTTTTA ATGAGAACAA AGCCTGGAAC
CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT TCCAGCCTGC TGAAGATATA CAGTAAAGAA
GAACAGGATT TCTTAAAGCT GGTTAAGTCC TATCACTGGA TGGGACTAGT CCAGAGCCCA
GCAAATGGCT CCTGGCAGTG GGAAGATGGC TCCTCTCTAT CACCCAACGA GTTAACACTG
GTGAAAACAC CAAGCGGAAC CTGTGCTGTC TATGGCTCAA GCTTTAAGGC CTACACAGAA
GACTGTTCAA ATCCAAACAC ATACATCTGT ATGAAAAGGG CAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqXP_006237139.1
CDS132..779
Misc Feature(1)264..770(+)
Misc Feature(2)423..767(+)
Misc Feature(3)582..728(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version XM_006237077
Organism Rattus norvegicus (Norway rat)
Definition PREDICTED: Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006237077

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAGCAAAT GCCATAATTA TGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAGCAT 
CAGAAACAAA GATCTGCACT CCCTACCAGC CGACCTGGAG AGAATGGTAT CATAAGACGA
AGGTCTTCTA TAGAAGAACT CAAAATATCT CCACTGTTCG TTGTTCGAGT CCTTGTTGCA
GCCATGACCA TTCGCTTCAC CGTTATCACA TTGACATGGC TTGCTGTTTT CATAACTTTG
TTATGCAACA AGGAAGTTTC AGTTTCCTCA AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC
GACTGGATAT GTCACAGAAA CAACTGTTAC CAATTTTTTA ATGAGAACAA AGCCTGGAAC
CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT TCCAGCCTGC TGAAGATATA CAGTAAAGAA
GAACAGGATT TCTTAAAGCT GGTTAAGTCC TATCACTGGA TGGGACTAGT CCAGAGCCCA
GCAAATGGCT CCTGGCAGTG GGAAGATGGC TCCTCTCTAT CACCCAACGA GTTAACACTG
GTGAAAACAC CAAGCGGAAC CTGTGCTGTC TATGGCTCAA GCTTTAAGGC CTACACAGAA
GACTGTTCAA ATCCAAACAC ATACATCTGT ATGAAAAGGG CAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa13224
Accession Version NM_133512.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 648bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 10-AUG-2014
Organism Rattus norvegicus (Norway rat)
Product NKG2-D type II integral membrane protein
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FQ241294.1 and AF009511.1. On Jul 25, 2012 this sequence version replaced gi:19424149. Summary: functions as a natural cytotoxicity receptor to stimulate perforin-mediated elimination of ligand-expressing tumor cells [RGD, Feb 2006]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: FQ241294.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
ATGAGCAAAT GCCATAATTA TGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAGCAT 
CAGAAACAAA GATCTGCACT CCCTACCAGC CGACCTGGAG AGAATGGTAT CATAAGACGA
AGGTCTTCTA TAGAAGAACT CAAAATATCT CCACTGTTCG TTGTTCGAGT CCTTGTTGCA
GCCATGACCA TTCGCTTCAC CGTTATCACA TTGACATGGC TTGCTGTTTT CATAACTTTG
TTATGCAACA AGGAAGTTTC AGTTTCCTCA AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC
GACTGGATAT GTCACAGAAA CAACTGTTAC CAATTTTTTA ATGAGAACAA AGCCTGGAAC
CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT TCCAGCCTGC TGAAGATATA CAGTAAAGAA
GAACAGGATT TCTTAAAGCT GGTTAAGTCC TATCACTGGA TGGGACTAGT CCAGAGCCCA
GCAAATGGCT CCTGGCAGTG GGAAGATGGC TCCTCTCTAT CACCCAACGA GTTAACACTG
GTGAAAACAC CAAGCGGAAC CTGTGCTGTC TATGGCTCAA GCTTTAAGGC CTACACAGAA
GACTGTTCAA ATCCAAACAC ATACATCTGT ATGAAAAGGG CAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_598196.1
CDS94..741
Misc Feature(1)4..6(+)
Misc Feature(2)226..732(+)
Misc Feature(3)247..315(+)
Misc Feature(4)385..729(+)
Misc Feature(5)544..690(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_133512
Organism Rattus norvegicus (Norway rat)
Definition Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_133512

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAGCAAAT GCCATAATTA TGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAGCAT 
CAGAAACAAA GATCTGCACT CCCTACCAGC CGACCTGGAG AGAATGGTAT CATAAGACGA
AGGTCTTCTA TAGAAGAACT CAAAATATCT CCACTGTTCG TTGTTCGAGT CCTTGTTGCA
GCCATGACCA TTCGCTTCAC CGTTATCACA TTGACATGGC TTGCTGTTTT CATAACTTTG
TTATGCAACA AGGAAGTTTC AGTTTCCTCA AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC
GACTGGATAT GTCACAGAAA CAACTGTTAC CAATTTTTTA ATGAGAACAA AGCCTGGAAC
CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT TCCAGCCTGC TGAAGATATA CAGTAAAGAA
GAACAGGATT TCTTAAAGCT GGTTAAGTCC TATCACTGGA TGGGACTAGT CCAGAGCCCA
GCAAATGGCT CCTGGCAGTG GGAAGATGGC TCCTCTCTAT CACCCAACGA GTTAACACTG
GTGAAAACAC CAAGCGGAAC CTGTGCTGTC TATGGCTCAA GCTTTAAGGC CTACACAGAA
GACTGTTCAA ATCCAAACAC ATACATCTGT ATGAAAAGGG CAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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