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SNF1 cDNA ORF clone, Saccharomyces cerevisiae S288c

Gene Symbol SNF1
Entrez Gene ID 852088
Full Name AMP-activated serine/threonine-protein kinase catalytic subunit SNF1
Synonyms CAT1, CCR1, GLC2, HAF3, PAS14
General protein information
Names
Snf1p
NP_010765.3
  • AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288c
Genome

IV

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_001180785 NP_010765 AMP-activated serine/threonine-protein kinase catalytic subunit SNF1

KEGG
sce04113 Meiosis - yeast



Component

ID Name Evidence
GO:0000324 fungal-type vacuole IPI
GO:0005634 nucleus IDA
GO:0005634 nucleus IEA
GO:0005634 nucleus IPI
GO:0005641 nuclear envelope lumen IDA
GO:0005737 cytoplasm IDA
GO:0005737 cytoplasm IPI
GO:0005739 mitochondrion IDA
GO:0016020 membrane IEA
GO:0031588 AMP-activated protein kinase complex IDA
GO:0031588 AMP-activated protein kinase complex IPI
GO:0031965 nuclear membrane IEA

Function

ID Name Evidence
GO:0000166 nucleotide binding IEA
GO:0004672 protein kinase activity IEA
GO:0004674 protein serine/threonine kinase activity IEA
GO:0004679 AMP-activated protein kinase activity IDA
GO:0004679 AMP-activated protein kinase activity IMP
GO:0005524 ATP binding IEA
GO:0016301 kinase activity IEA
GO:0016740 transferase activity IEA

Process

ID Name Evidence
GO:0001302 replicative cell aging IGI
GO:0001302 replicative cell aging IMP
GO:0001403 invasive growth in response to glucose limitation IMP
GO:0005975 carbohydrate metabolic process IEA
GO:0006109 regulation of carbohydrate metabolic process IGI
GO:0006109 regulation of carbohydrate metabolic process IPI
GO:0006109 regulation of carbohydrate metabolic process TAS
GO:0006468 protein phosphorylation IDA
GO:0006468 protein phosphorylation IEA
GO:0006995 cellular response to nitrogen starvation IDA
GO:0007124 pseudohyphal growth IMP
GO:0007155 cell adhesion IMP
GO:0007165 signal transduction TAS
GO:0017148 negative regulation of translation IGI
GO:0017148 negative regulation of translation IMP
GO:0042710 biofilm formation IMP
GO:0045722 positive regulation of gluconeogenesis IMP

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SNF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OSi01545 NM_001180785 Saccharomyces cerevisiae S288c AMP-activated serine/threonine-protein kinase catalytic subunit SNF1 (SNF1), partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $489.00

*Business Day
**You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.


CloneID OSi01545
Accession Version NM_001180785.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1902bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Saccharomyces cerevisiae S288c
Product AMP-activated serine/threonine-protein kinase catalytic subunit SNF1
Comment REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced gi:392859851. COMPLETENESS: incomplete on both ends.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGAGCAGTA ACAACAACAC AAACACAGCA CCTGCCAATG CAAATTCTAG CCACCACCAC 
CACCATCACC ACCATCACCA CCACCATCAC GGTCATGGCG GAAGCAACTC GACGCTAAAC
AATCCCAAGT CGTCCTTAGC GGATGGTGCA CATATCGGGA ACTACCAAAT CGTCAAAACG
CTGGGAGAGG GGTCCTTTGG TAAAGTTAAA TTGGCATATC ATACCACTAC GGGCCAAAAA
GTTGCTCTAA AAATCATTAA TAAGAAGGTT TTGGCAAAGA GTGATATGCA GGGCAGAATT
GAAAGAGAAA TATCTTATCT GAGACTCTTA AGACACCCCC ACATCATCAA ACTGTATGAT
GTTATCAAAT CCAAAGATGA AATCATTATG GTTATAGAGT ACGCCGGGAA CGAATTGTTT
GACTATATTG TTCAGAGAGA CAAAATGAGC GAGCAAGAGG CAAGAAGATT TTTCCAGCAG
ATCATCAGTG CCGTCGAGTA CTGCCATAGG CACAAAATTG TCCATAGAGA TCTGAAGCCT
GAAAACTTAC TACTAGATGA GCATCTGAAT GTAAAGATTG CCGATTTTGG TTTGTCAAAC
ATCATGACTG ATGGTAATTT CTTAAAGACT TCTTGTGGTT CTCCCAATTA TGCGGCTCCT
GAAGTTATCA GCGGTAAGCT GTACGCAGGC CCAGAAGTGG ACGTGTGGTC ATGTGGGGTT
ATCCTTTATG TTATGCTTTG TCGTCGTCTA CCGTTTGACG ATGAAAGCAT CCCAGTGCTT
TTCAAGAATA TCAGCAACGG TGTTTACACC TTGCCTAAAT TTTTATCTCC TGGAGCTGCT
GGGCTAATCA AAAGAATGTT AATCGTTAAT CCATTGAACA GAATAAGCAT TCATGAAATT
ATGCAAGACG ATTGGTTCAA AGTTGACCTG CCAGAATATC TACTTCCACC AGATTTGAAA
CCACACCCAG AAGAAGAGAA TGAAAATAAT GACTCAAAAA AGGATGGCAG CAGCCCAGAT
AACGATGAAA TTGATGACAA CCTTGTCAAT ATTTTATCAT CGACCATGGG TTACGAAAAA
GACGAGATTT ATGAGTCCTT AGAATCATCA GAAGACACTC CTGCATTCAA CGAAATTAGG
GACGCGTACA TGTTGATTAA GGAGAATAAA TCTTTGATCA AGGATATGAA GGCAAACAAA
AGCGTCAGTG ATGAACTGGA TACCTTTCTG TCCCAGTCAC CTCCAACTTT TCAACAACAA
AGCAAATCCC ATCAAAAGAG TCAAGTAGAT CATGAAACTG CCAAGCAACA CGCAAGAAGG
ATGGCAAGTG CTATCACTCA ACAAAGGACA TATCACCAAT CACCCTTCAT GGATCAGTAT
AAAGAAGAAG ACTCTACAGT TTCCATTTTG CCTACATCTT TACCTCAGAT CCACAGAGCT
AATATGTTAG CACAAGGTTC GCCAGCTGCC TCTAAAATAT CTCCTCTTGT AACGAAAAAA
TCTAAAACGA GATGGCATTT TGGTATACGA TCTCGCTCAT ATCCATTAGA CGTTATGGGT
GAAATTTATA TTGCCTTGAA GAATTTGGGT GCCGAATGGG CCAAGCCATC TGAAGAGGAT
TTATGGACTA TCAAATTAAG GTGGAAATAT GATATTGGAA ACAAGACAAA CACTAATGAA
AAAATACCTG ATTTAATGAA AATGGTAATT CAATTATTTC AAATTGAAAC CAATAATTAT
TTGGTGGATT TCAAATTTGA CGGCTGGGAA AGTAGTTATG GAGATGATAC TACTGTTTCT
AATATTTCTG AAGATGAAAT GAGTACTTTT TCAGCCTACC CATTTTTACA TTTAACAACA
AAACTAATTA TGGAATTAGC CGTTAACAGT CAAAGCAATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_010765.3
CDS1..1902
Misc Feature(1)154..918(+)
Misc Feature(2)163..918(+)
Misc Feature(3)181..753(+)
Misc Feature(4)181..585(+)
Misc Feature(5)412..753(+)
Misc Feature(6)580..648(+)
Misc Feature(7)604..876(+)
Misc Feature(8)1030..1185(+)
Misc Feature(9)1504..1878(+)
Misc Feature(10)1507..1872(+)
Misc Feature(11)1648..1851(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001180785
Organism Saccharomyces cerevisiae S288c
Definition Saccharomyces cerevisiae S288c AMP-activated serine/threonine-protein kinase catalytic subunit SNF1 (SNF1), partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001180785

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGAGCAGTA ACAACAACAC AAACACAGCA CCTGCCAATG CAAATTCTAG CCACCACCAC 
CACCATCACC ACCATCACCA CCACCATCAC GGTCATGGCG GAAGCAACTC GACGCTAAAC
AATCCCAAGT CGTCCTTAGC GGATGGTGCA CATATCGGGA ACTACCAAAT CGTCAAAACG
CTGGGAGAGG GGTCCTTTGG TAAAGTTAAA TTGGCATATC ATACCACTAC GGGCCAAAAA
GTTGCTCTAA AAATCATTAA TAAGAAGGTT TTGGCAAAGA GTGATATGCA GGGCAGAATT
GAAAGAGAAA TATCTTATCT GAGACTCTTA AGACACCCCC ACATCATCAA ACTGTATGAT
GTTATCAAAT CCAAAGATGA AATCATTATG GTTATAGAGT ACGCCGGGAA CGAATTGTTT
GACTATATTG TTCAGAGAGA CAAAATGAGC GAGCAAGAGG CAAGAAGATT TTTCCAGCAG
ATCATCAGTG CCGTCGAGTA CTGCCATAGG CACAAAATTG TCCATAGAGA TCTGAAGCCT
GAAAACTTAC TACTAGATGA GCATCTGAAT GTAAAGATTG CCGATTTTGG TTTGTCAAAC
ATCATGACTG ATGGTAATTT CTTAAAGACT TCTTGTGGTT CTCCCAATTA TGCGGCTCCT
GAAGTTATCA GCGGTAAGCT GTACGCAGGC CCAGAAGTGG ACGTGTGGTC ATGTGGGGTT
ATCCTTTATG TTATGCTTTG TCGTCGTCTA CCGTTTGACG ATGAAAGCAT CCCAGTGCTT
TTCAAGAATA TCAGCAACGG TGTTTACACC TTGCCTAAAT TTTTATCTCC TGGAGCTGCT
GGGCTAATCA AAAGAATGTT AATCGTTAAT CCATTGAACA GAATAAGCAT TCATGAAATT
ATGCAAGACG ATTGGTTCAA AGTTGACCTG CCAGAATATC TACTTCCACC AGATTTGAAA
CCACACCCAG AAGAAGAGAA TGAAAATAAT GACTCAAAAA AGGATGGCAG CAGCCCAGAT
AACGATGAAA TTGATGACAA CCTTGTCAAT ATTTTATCAT CGACCATGGG TTACGAAAAA
GACGAGATTT ATGAGTCCTT AGAATCATCA GAAGACACTC CTGCATTCAA CGAAATTAGG
GACGCGTACA TGTTGATTAA GGAGAATAAA TCTTTGATCA AGGATATGAA GGCAAACAAA
AGCGTCAGTG ATGAACTGGA TACCTTTCTG TCCCAGTCAC CTCCAACTTT TCAACAACAA
AGCAAATCCC ATCAAAAGAG TCAAGTAGAT CATGAAACTG CCAAGCAACA CGCAAGAAGG
ATGGCAAGTG CTATCACTCA ACAAAGGACA TATCACCAAT CACCCTTCAT GGATCAGTAT
AAAGAAGAAG ACTCTACAGT TTCCATTTTG CCTACATCTT TACCTCAGAT CCACAGAGCT
AATATGTTAG CACAAGGTTC GCCAGCTGCC TCTAAAATAT CTCCTCTTGT AACGAAAAAA
TCTAAAACGA GATGGCATTT TGGTATACGA TCTCGCTCAT ATCCATTAGA CGTTATGGGT
GAAATTTATA TTGCCTTGAA GAATTTGGGT GCCGAATGGG CCAAGCCATC TGAAGAGGAT
TTATGGACTA TCAAATTAAG GTGGAAATAT GATATTGGAA ACAAGACAAA CACTAATGAA
AAAATACCTG ATTTAATGAA AATGGTAATT CAATTATTTC AAATTGAAAC CAATAATTAT
TTGGTGGATT TCAAATTTGA CGGCTGGGAA AGTAGTTATG GAGATGATAC TACTGTTTCT
AATATTTCTG AAGATGAAAT GAGTACTTTT TCAGCCTACC CATTTTTACA TTTAACAACA
AAACTAATTA TGGAATTAGC CGTTAACAGT CAAAGCAATT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV
Nature 387 (6632 SUPPL), 75-78 (1997)
Jacq,C., Alt-Morbe,J., Andre,B., Arnold,W., Bahr,A., Ballesta,J.P., Bargues,M., Baron,L., Becker,A., Biteau,N., Blocker,H., Blugeon,C., Boskovic,J., Brandt,P., Bruckner,M., Buitrago,M.J., Coster,F., Delaveau,T., del Rey,F., Dujon,B., Eide,L.G., Garcia-Cantalejo,J.M., Goffeau,A., Gomez-Peris,A., Zaccaria,P. et al.


book

Life with 6000 genes
Science 274 (5287), 546 (1996)
Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B., Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M., Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and Oliver,S.G.


book

Direct Submission
Submitted (15-JUL-2015) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA


book

Direct Submission
Submitted (16-JAN-2015) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


book

Direct Submission
Submitted (06-FEB-2013) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


book

Direct Submission
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


book

Direct Submission
Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


book

Direct Submission
Submitted (11-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


 
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