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YJR029W cDNA ORF clone, Saccharomyces cerevisiae S288c

Gene Symbol YJR029W
Entrez Gene ID 853486
Full Name gag-pol fusion protein
Synonyms
General protein information
Names
hypothetical protein
NP_012562.1
  • Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is processed to make a nucleocapsid-like protein (Gag), reverse transcriptase (RT), protease (PR), and integrase (IN); similar to retroviral genes
Gene Type protein-coding
Organism Saccharomyces cerevisiae S288c
Genome

X

Summary lac of sum
Disorder MIM:

Disorder Html:

mRNA and Protein(s)

mRNA Protein Name
NM_001181687 NP_012562 gag-pol fusion protein




Component

ID Name Evidence
GO:0000943 retrotransposon nucleocapsid ISS
GO:0005634 nucleus IEA
GO:0005737 cytoplasm IEA

Function

ID Name Evidence
GO:0000166 nucleotide binding IEA
GO:0003676 nucleic acid binding IEA
GO:0003677 DNA binding IEA
GO:0003723 RNA binding IEA
GO:0003723 RNA binding ISS
GO:0003824 catalytic activity IEA
GO:0003887 DNA-directed DNA polymerase activity IEA
GO:0003887 DNA-directed DNA polymerase activity ISS
GO:0003964 RNA-directed DNA polymerase activity IEA
GO:0003964 RNA-directed DNA polymerase activity ISS
GO:0004190 aspartic-type endopeptidase activity IEA
GO:0004518 nuclease activity IEA
GO:0004519 endonuclease activity IEA
GO:0004523 ribonuclease H activity IEA
GO:0004540 ribonuclease activity ISS
GO:0005524 ATP binding IEA
GO:0008233 peptidase activity IEA
GO:0008233 peptidase activity ISS
GO:0016740 transferase activity IEA
GO:0016779 nucleotidyltransferase activity IEA
GO:0016787 hydrolase activity IEA
GO:0046872 metal ion binding IEA

Process

ID Name Evidence
GO:0006310 DNA recombination IEA
GO:0006508 proteolysis IEA
GO:0015074 DNA integration IEA
GO:0032196 transposition IEA
GO:0032197 transposition, RNA-mediated ISS
GO:0046797 viral procapsid maturation IEA

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following YJR029W gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YJR029W cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
NM_001181687 Saccharomyces cerevisiae S288c gag-pol fusion protein partial mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD Quote Price

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee that the protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID
Accession Version NM_001181687.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Saccharomyces cerevisiae S288c
Product
Comment REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Feb 18, 2015 this sequence version replaced gi:296146038. COMPLETENESS: incomplete on both ends.

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq
CDS
Misc Feature(1)49..342(+)
Misc Feature(2)1988..2347(+)
Misc Feature(3)3794..4483(+)
Misc Feature(4)4817..5227(+)
Misc Feature(5)4829..5218(+)
Misc Feature(6)4829..5206(+)
Translation
Position Chain Variation Link

Target ORF information:

RefSeq Version NM_001181687
Organism Saccharomyces cerevisiae S288c
Definition Saccharomyces cerevisiae S288c gag-pol fusion protein partial mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001181687

ORF Insert Sequence:

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Life with 6000 genes
Science 274 (5287), 546 (1996)
Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B., Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M., Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and Oliver,S.G.


book

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X
EMBO J. 15 (9), 2031-2049 (1996)
Galibert,F., Alexandraki,D., Baur,A., Boles,E., Chalwatzis,N., Chuat,J.C., Coster,F., Cziepluch,C., De Haan,M., Domdey,H., Durand,P., Entian,K.D., Gatius,M., Goffeau,A., Grivell,L.A., Hennemann,A., Herbert,C.J., Heumann,K., Hilger,F., Hollenberg,C.P., Huang,M.E., Jacq,C., Jauniaux,J.C., Katsoulou,C., Karpfinger-Hartl,L. et al.


book

Direct Submission
Submitted (15-JUL-2015) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA


book

Direct Submission
Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


book

Direct Submission
Submitted (12-APR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


book

Direct Submission
Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA


 
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