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TNF cDNA ORF clone, Sus scrofa (pig)

Gene Symbol TNF
Entrez Gene ID 397086
Full Name tumor necrosis factor
Synonyms TNFa
General protein information
Preferred Names
tumor necrosis factor
Names
tumor necrosis factor
TNF-a
TNF-alpha
cachectin
tumor-necrosis factor
tumor necrosis factor alpha
tumor necrosis factor (TNF superfamily, member 2)
tumor necrosis factor ligand superfamily member 2
Gene Type protein-coding
Organism Sus scrofa (pig)
Genome

7

7|7p11-q11

Summary lac of sum

mRNA and Protein(s)

mRNA Protein Name
NM_214022 NP_999187 tumor necrosis factor

KEGG
ssc04060 Cytokine-cytokine receptor interaction
ssc04010 MAPK signaling pathway
ssc04150 mTOR signaling pathway
ssc04210 Apoptosis
ssc04640 Hematopoietic cell lineage
ssc04350 TGF-beta signaling pathway
ssc04612 Antigen processing and presentation
ssc05010 Alzheimer's disease
ssc04620 Toll-like receptor signaling pathway
ssc04930 Type II diabetes mellitus
ssc04940 Type I diabetes mellitus
ssc04660 T cell receptor signaling pathway
ssc04664 Fc epsilon RI signaling pathway
ssc04920 Adipocytokine signaling pathway
ssc05330 Allograft rejection
ssc04650 Natural killer cell mediated cytotoxicity
ssc05014 Amyotrophic lateral sclerosis (ALS)
ssc05322 Systemic lupus erythematosus
ssc05332 Graft-versus-host disease
ssc05310 Asthma
ssc05410 Hypertrophic cardiomyopathy (HCM)
ssc05414 Dilated cardiomyopathy
ssc04621 NOD-like receptor signaling pathway
ssc04622 RIG-I-like receptor signaling pathway
ssc05142 Chagas disease (American trypanosomiasis)
ssc05140 Leishmaniasis
ssc05146 Amoebiasis
ssc05145 Toxoplasmosis
ssc05144 Malaria
ssc05160 Hepatitis C
ssc04380 Osteoclast differentiation
ssc05323 Rheumatoid arthritis
ssc05143 African trypanosomiasis
ssc05152 Tuberculosis
ssc05133 Pertussis
ssc05166 HTLV-I infection
ssc05164 Influenza A
ssc05168 Herpes simplex infection
ssc05134 Legionellosis
ssc05205 Proteoglycans in cancer
ssc05161 Hepatitis B
ssc04064 NF-kappa B signaling pathway
ssc05321 Inflammatory bowel disease (IBD)
ssc04071 Sphingolipid signaling pathway
ssc04668 TNF signaling pathway
ssc04932 Non-alcoholic fatty liver disease (NAFLD)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OSe30520 NM_214022 Sus scrofa tumor necrosis factor (TNF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $174.30
$249.00

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).


CloneID OSe30520
Accession Version NM_214022.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 01-FEB-2014
Organism Sus scrofa (pig)
Product tumor necrosis factor
Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X54859.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57321.1, EU682384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS198675, ERS198681 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AGAGCATGAT CCGAGACGTG GAGCTGGCGG AGGAGGCGCT CGCCAAGAAG 
GCCGGGGGCC CCCAGGGCTC CAGGAGGTGC CTGTGCCTCA GCCTCTTCTC CTTCCTCCTG
GTCGCAGGAG CCACCACGCT CTTCTGCCTA CTGCACTTCG AGGTTATCGG CCCCCAGAAG
GAAGAGTTTC CAGCTGGCCC CTTGAGCATC AACCCTCTGG CCCAAGGACT CAGATCATCG
TCTCAAACCT CAGATAAGCC CGTCGCCCAC GTTGTAGCCA ATGTCAAAGC CGAGGGACAG
CTCCAATGGC AGAGTGGGTA TGCCAATGCC CTCCTGGCCA ACGGCGTGAA GCTGAAAGAC
AACCAGCTGG TGGTGCCGAC AGATGGGCTG TACCTCATCT ACTCCCAGGT CCTCTTCAGG
GGCCAAGGCT GCCCTTCCAC CAACGTTTTC CTCACTCACA CCATCAGCCG CATCGCCGTC
TCCTACCAGA CCAAGGTCAA CCTCCTCTCT GCCATCAAGA GCCCTTGCCA GAGGGAGACC
CCCGAGGGGG CCGAGGCCAA GCCCTGGTAC GAACCCATCT ACCTGGGAGG GGTCTTCCAG
CTGGAGAAGG ATGATCGACT CAGTGCCGAG ATCAACCTGC CCGACTATCT GGACTTTGCT
GAATCTGGGC AGGTCTATTT TGGGATCATT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeqNP_999187.1
CDS184..882
Misc Feature(1)67..69(+)
Misc Feature(2)283..288(+)
Misc Feature(3)289..351(+)
Misc Feature(4)298..303(+)
Misc Feature(5)328..333(+)
Misc Feature(6)334..339(+)
Misc Feature(7)409..414(+)
Misc Feature(8)442..873(+)
Misc Feature(9)451..873(+)
Misc Feature(10)493..675(+)
Translation
Position Chain Variation Link
103 103 a, g dbSNP:327105809
134 134 a, g dbSNP:346300555
318 318 c, g dbSNP:332417852
489 489 c, t dbSNP:80945725
735 735 a, g dbSNP:80967540
900 900 a, g dbSNP:80784448
1000 1000 a, g dbSNP:80860077
1068 1068 c, t dbSNP:80859275

Target ORF information:

RefSeq Version NM_214022
Organism Sus scrofa (pig)
Definition Sus scrofa tumor necrosis factor (TNF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_214022

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCACTG AGAGCATGAT CCGAGACGTG GAGCTGGCGG AGGAGGCGCT CGCCAAGAAG 
GCCGGGGGCC CCCAGGGCTC CAGGAGGTGC CTGTGCCTCA GCCTCTTCTC CTTCCTCCTG
GTCGCAGGAG CCACCACGCT CTTCTGCCTA CTGCACTTCG AGGTTATCGG CCCCCAGAAG
GAAGAGTTTC CAGCTGGCCC CTTGAGCATC AACCCTCTGG CCCAAGGACT CAGATCATCG
TCTCAAACCT CAGATAAGCC CGTCGCCCAC GTTGTAGCCA ATGTCAAAGC CGAGGGACAG
CTCCAATGGC AGAGTGGGTA TGCCAATGCC CTCCTGGCCA ACGGCGTGAA GCTGAAAGAC
AACCAGCTGG TGGTGCCGAC AGATGGGCTG TACCTCATCT ACTCCCAGGT CCTCTTCAGG
GGCCAAGGCT GCCCTTCCAC CAACGTTTTC CTCACTCACA CCATCAGCCG CATCGCCGTC
TCCTACCAGA CCAAGGTCAA CCTCCTCTCT GCCATCAAGA GCCCTTGCCA GAGGGAGACC
CCCGAGGGGG CCGAGGCCAA GCCCTGGTAC GAACCCATCT ACCTGGGAGG GGTCTTCCAG
CTGGAGAAGG ATGATCGACT CAGTGCCGAG ATCAACCTGC CCGACTATCT GGACTTTGCT
GAATCTGGGC AGGTCTATTT TGGGATCATT GCCCTGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Peripheral tumor necrosis factor alpha regulation of adipose tissue metabolism and adipokine gene expression in neonatal pigs
Vet. Res. Commun. 37 (1), 1-10 (2013)
Ramsay TG, Stoll MJ, Conde-Aguilera JA and Caperna TJ.


book

Effects of high tidal volume mechanical ventilation on production of cytokines, iNOS, and MIP-1beta proteins in pigs
Exp. Lung Res. 39 (1), 1-8 (2013)
Vobruba V, Klimenko OV, Kobr J, Cerna O, Pokorna P, Mikula I, Hridel J, Brantova O and Martasek P.


book

High-volume hemofiltration reduces the expression of myocardial tumor necrosis factor-alpha in septic shock pigs
Artif Organs 37 (2), 196-202 (2013)
Li C, Zhang P, Cheng X and Chen J.


book

Amino acid residues in the non-structural protein 1 of porcine reproductive and respiratory syndrome virus involved in down-regulation of TNF-alpha expression in vitro and attenuation in vivo
Virology 432 (2), 241-249 (2012)
Subramaniam S, Beura LK, Kwon B, Pattnaik AK and Osorio FA.


book

CRF induces intestinal epithelial barrier injury via the release of mast cell proteases and TNF-alpha
PLoS ONE 7 (6), E39935 (2012)
Overman EL, Rivier JE and Moeser AJ.


book

Assignment of the porcine tumour necrosis factor alpha and beta genes to the chromosome region 7p11-q11 by in situ hybridization
Anim. Genet. 23 (3), 267-271 (1992)
Solinas S, Pauli U, Kuhnert P, Peterhans E and Fries R.


book

The porcine tumor necrosis factor-encoding genes: sequence and comparative analysis
Gene 102 (2), 171-178 (1991)
Kuhnert P, Wuthrich C, Peterhans E and Pauli U.


book

Mapping and genetic organization of the TNF genes in the swine Mhc
Immunogenetics 34 (4), 257-260 (1991)
Chardon P, Nunes M, Dezeure F, Andres-Cara D and Vaiman M.


book

Gene sequence of porcine tumor necrosis factor alpha
Nucleic Acids Res. 18 (18), 5564 (1990)
Drews RT, Coffee BW, Prestwood AK and McGraw RA.


book

Porcine tumor necrosis factor alpha: cloning with the polymerase chain reaction and determination of the nucleotide sequence
Gene 81 (1), 185-191 (1989)
Pauli U, Beutler B and Peterhans E.


 
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