Gene Synthesis Service

Advantages

• Free codon optimization - Proprietary OptimumGene™ codon optimization technology can comprehensively optimize DNA sequences to significantly increase protein expression in any host.
• Any gene in any vector - CloneEZ^® seamless cloning technology can accurately and efficiently fuse your gene into any site on any vector in less than 30 minutes.
• Guaranteed sequence - Proprietary Gene-on-Demand^® gene synthesis platform to enable mutation-free DNA synthesis for genes of any length and any complexity.
• Express gene synthesis – GenScript express gene synthesis delivers your custom designed gene sequence in as few as 4 business days. Choose from 2 flexible service options, Rush or Fast, to meet all of you research deadlines and goals.
• CloneReady™ Gene Collection - CloneReady™ Gene Collection covers over 120,000 popular genes at most competitive price as low as $0.29/bp.
• One-stop solutions - Comprehensive downstream services can save your time on material transfer.
• Cost effective - Ease your budget crunch with competitive prices supported by economy-of-scale.

Price List

• 1. The minimum charge for each DNA synthesis project is $159.
• 2. Mutagenesis is starting from $149/mutation.
• 3. Customers retain all rights to the sequence data and related intellectual property.

Vector Information

Turnaround Time

Delivery Specifications

• 4 μg of lyophilized plasmid containing your gene insert
• Sequence chromatograms covering your gene (electronic)
• Construct map for the plasmid (electronic)
• Quality assurance certificate

Have a question about your custom gene synthesis^TM Please check our FAQs or contact our customer service representative for assistance.

Special Partnership Program

GenScript has established a special strategic partnership program for Gene-on-Demand^® gene synthesis service to clients worldwide. In particular, this new program offers the service at special and competitive prices to our valued clients who want to establish a long-term preferred vendor contract with GenScript. We are willing to work with you in a flexible manner within your budget to achieve your goals for the years to come. With our solid organic growth and financial performance, GenScript assures reliable and sustainable services.

For more information, please call us at 1-732-885-9188, or email us at gene@genscript.com. Our gene service representatives are available 24 hours a day, Monday through Friday to assist you.

OptimumGene™ Codon Optimization Technology

GenScript's OptimumGene™ Codon Optimization system is a proprietary gene optimization technology that can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of productivity in any given expression system. The OptimumGene™ algorithm takes into consideration of a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation.

Advantages

• Significant increase in protein expression
• Fruitful results in even the most difficult sequences
• Comprehensive usage tables for optimization in any host

GenScript has accumulated years of experience in codon optimization and gene synthesis. Our codon optimization system has optimized over 50,000 sequences in all major expression systems.

Fig. 1. Based on our historical records, the OptimumGene™ codon optimization has optimized over 50,000 sequences in all major expression systems.

   

Fig. 2. GenScript OptimumGene™ codon optimization genes increased the yield of expression (8 out of 10 genes) and the degree of solubility in some cases (6 out of 10 genes) compared to the native genes.

Reference: Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O. Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study. Protein Expr Purif. May 2008; 59(1): 94-102

Fig. 3. GenScript's OptimumGene™ codon optimization delivered 20 times higher expression level for protein β compared to non-optimized native gene sequence. The expression level was 13 times more than that from Competitor GA's optimization method.

More Case Studies

Applications

GenScript's custom gene synthesis technology is extremely versatile and applicable in many different research areas, such as cDNA construction, heterogenous protein expression, and the creation of gene variants and recombinant antibodies and synthetic biology. GenScript's gene synthesis service comes with our advanced OptimumGene™ codon optimization technology, which can dramatically increase heterogeneous protein expression, at no additional charge.

	Synthetic Biology: Synthetic biology aims to design and build artificial life or to modify existing natural molecules to carry out novel tasks. In general, the synthetic goal is to solve problems that are not easily encountered though analysis and observation alone. The potential benefits of synthetic biology include developing new microorganisms to treat disease, provide energy, food, and to detect and break down environmental pollutants in the soil, air and water. Mature develop gene synthesis technology enables scientists to make genes artificial, combining with the principles of modern engineering, scientists can now design organisms that do new things—like produce biofuels or excrete the precursors of medical drugs.
	Codon Optimization: Not all host expression systems share the same codon bias. The expression of, say, a human gene in E. coli can increase dramatically when the codon frequency of that gene is matched to that of the host system, but that is only the first step of GenScript's total gene optimization process. GenScript's proprietary OptimumGene™ Gene Design Technology can typically increase protein expression level up to 30-fold Case Study, provided that the protein expression and purification methods are appropriate. More about Codon Optimization
	TALEs: Transcription Activator-Like Effectors (TALEs) are highly-specific, high-affinity DNA-binding proteins that can be fused to a functional enzyme. Together, the two proteins have the ability to target and edit nearly any site in a genome. Read more to learn about TALEs and why GenScript gene synthesis is the easy way to generate custom designed TALEs. More about TALEs
	Chimeric Antibodies:Human-mouse recombinant antibodies retain the specificity of the original murine variable region but assume the effector function of the human constant region. GenScript's Chimeric Antibody Generation Service has developed advanced technology to rapidly express full length recombinant antibodies with the desired traits. More about Recombinant Antibodies
	cDNA Design and Constructs: GenScript gene synthesis technology allows for the design of high-quality genes and cDNAs at highly competitive prices, and the direct production of high-quality cDNAs for cDNA libraries and other purposes. More about cDNA Design and cDNA Constructs
	Gene Variants and SNPs: Most alternative splice methods are unwieldy and can have unpredictable results.GenScript gene modification and gene synthesis incorporates rigorous regulation and verification of alternative splice products. More about Gene Variants and SNPs and Gene Modification
	DNA Vaccines and Vectors: Since the production of an effective DNA vaccine involves not only the sequence itself but also the target, delivery system, expression, regulation, and inhibition of the gene therapy product, GenScript's one stop for gene and protein services is your best bet. More about DNA Vaccines and Vectors
	Microarray-Ready cDNA: Reverse transcription bias and other problems can alter the relative amounts of cDNA measured by microarray assays. GenScript's advanced gene synthesis technology includes several means of countering these issues. More about Microarray-Ready cDNA
	Predicted Gene: The primary reason to generate predicted gene structure databases is to provide positional cloners, gene hunters and other products along with the gene candidates observed in finished and unfinished genomic sequences. GenScript specializes in custom gene synthesis and predicted gene synthesis based on advances in the understanding of gene variant function and synthesis, the biology of gene synthesis and new DNA manipulation and mutagenesis techniques. More about Synthesize Predicted Genes and cDNAs

Customer Testimonials

"I think GenScript provides absolutely excellent products in a timely fashion. I have primarily used GenScript to order codon optimized artificial genes. Ordering artificial genes on their website or via Email makes this especially facile because I can simply inquire about a gene, get a quote and then get a PO number. The whole process is fast and the turnaround time for obtaining the finished product is usually less than a month. I also think their technical support are wonderful people, they have been most helpful in facilitating the process. I am so impressed with them that I have recommended them to several of my colleagues."

― Dr. Maria Schumacher, The University of Texas MD Anderson Cancer Center, USA

"We repeatedly ordered synthetic genes from GenScript. In any case, the synthesis was exceptionally fast (usually within 7-10 business days), convenient, reliable and cost-effective. Customer service was exceptionally friendly and even approachable over Christmas vacation. For that reason, we can only recommend this service to any customer – wherever they may be located."

― Dr. Tilmann Buerckstuemmer, Austrian Academy of Sciences, Austria

"GenScript is the company that I have entrusted my project with. The delivery of your gene synthesis is on time and the OptimumGene™ technology on your Gene-on-Demand^® gene synthesis platform has increased my gene expression dramatically. I am very happy with my results and GenScript is always my first choice for gene services."

― Bin He, Bristol-Myers Squibb, USA

"I am glad that I have chosen gene synthesis over traditional cloning, it has saved me a lot of time. I would like to continue using your service in the future."

― Dr. Bin Shuai, Wichita State University, USA

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Newest List of Publications Cited the Use of Our Gene Synthesis Service

• Marcus E. Shin, Aikaterini Skokotas, and Edward Winter. The Cdk1 and Ime2 Protein Kinases Trigger Exit from Meiotic Prophase inSaccharomyces cerevisiae by Inhibiting the Sum1 Transcriptional Repressor. Mol. Cell. Biol. Jun 2010; 30(12): 2996 - 3003
  
  
• Gen He, Wenjie Luo, Peng Li, Chrisine Remmers, William J. Netzer, Joseph Hendrick, Karima Bettayeb, Marc Flajolet, Fred Gorelick, Lawrence P. Wennogle, and Paul Greengard. Gam-ma-secretase activating protein is a therapeutic target for Alzheimer's disease. Nature. July 2010; 467: 95 - 98
  
  
• David C. F. Sealey, Le Zheng, Michael A. S. Taboski, Jennifer Cruickshank, Mitsuhiko Ikura, and Lea A. Harrington. The N-terminus of hTERT contains a DNA-binding domain and is required for telomerase activity and cellular immortalization. Nucleic Acids Res. Apr 2010; 38(6): 2019 - 2035
  
  
• Ye Dee Tay, Julie M. Sidebotham, and Leonard Wu. Mph1 requires mismatch repair-independent and -dependent functions of MutSα to regulate crossover formation during homologous recombination repair. Nucleic Acids Res. Apr 2010; 38(6): 1889 - 1901
  
  
• Mookkan Prabakaran, Selvaraj Madhan, Nayana Prabhu, Jia Qiang, and Jimmy Kwang. Gastrointestinal delivery of baculovirus displaying influenza hemagglutinin protects mice against heterologous H5N1 infection. J. Virol. Apr 2010; 84(7): 3201 - 3209
  
  
• József Dobó, János Varga, Ráchel Sajó, Barbara M. Végh, Péter Gál, Péter Závodszky, and Ferenc Vonderviszt. Application of a Short, Disordered N-Terminal Flagellin Segment, a Fully Functional Flagellar Type III Export Signal, to Expression of Secreted Proteins. Appl. Envir. Microbiol. Feb 2010; 76(3): 891 - 899
  
  
• Ana Paula D. Ano Bom, Monica S. Freitas, Flavia S. Moreira, Danielly Ferraz, Daniel Sanches, Andre M. O. Gomes, Ana Paula Valente, Yraima Cordeiro, and Jerson L. Silva. The p53 Core Domain Is a Molten Globule at Low pH: FUNCTIONAL IMPLICATIONS OF A PARTIALLY UNFOLDED STRUCTURE. J. Biol. Chem. Jan 2010; 285(4): 2857 - 2866
  
  

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