| Guarantee |
Two positive clones to the given phospho-peptide with less than 30% cross-reactivity to the non-phospho-peptide by ELISA. Guarantee applies to peptides designed or guaranteed by GenScript. |
| Peptide synthesis |
| We will synthesize 10 mg of phospho-peptide and 5 mg non-phospho-peptide with up to 15 residues and 90% purity. 5 mg of free modified peptide and 3 mg of unmodified peptide will be delivered to the customer along with the final antibody product. |
|
| Phase I: Immunization and ELISA screening |
Conjugation: The modified peptide is conjugated to a carrier protein, such as KLH.
Immunization: 10 Balb/c mice are immunized and boosted with a standard immunization protocol.
Screening: Tail bleedings are screened by ELISA with phospho and non-phospho-peptide. |
| Phase II: Fusion and screening |
Fusion: Splenocyte fusions are performed on those mice that respond the best to the phospho-peptide in Phase I.
Expansion and screening: The hybridoma cells are screened by ELISA
with phospho and non-phospho-petide and all positive clones are
expanded into 24-well plates. The clones with best ELISA value and
specificity to phosphopeptide are selected for subcloning. |
| Phase III: Subcloning and delivery |
Subcloning: Hybridoma subclones are screened by ELISA with phospho
and non-phospho-petide. We subclone up to five positive parental
clones by limiting dilution, isotype the final
positive supernatants, and expand and cryopreserve two subclones
for each parental.
Delivery: Two vials of frozen cells or one flask of live cells
and 5 ml of culture supernatants for a selected subclone. We will
gladly provide subcloning for orders involving additional parental
clones for an additional fee.
|
| Shipping conditions |
The product is shipped on dry ice. |
| Timeline |
Peptides synthesis: 3-4 weeks
Phase I: 8-10 weeks
Phase II: 4-8 weeks
Phase III: 4-6 weeks
Total Time: 5-7 months |
