E.coli Protein Expression—SC1256 Economic Package ($1,750)
If customers could supply expression protocol of their
proteins, we recommend our Economic Package for Bacterial Expression, as a more economic solution.
| Milestones |
Specifications |
Deliverables |
Timeline1 |
| 1. Subcloning |
- Subcloning into up to 1 expression construct, under the choice of the client
Construct(s) report |
2 weeks |
|
| 2. Pilot expression and purification |
- Follow client's protocol about temperature, time course, inducer concentration
- Appropriate E.coli strain
- SDS-PAGE gel and/or Western blot
| Expression data |
1 week |
| 3. Protein expression and one-step purification |
- One strain from above
- Follow client's purification protocol
- QC by SDS-PAGE, Western blot2, and MALDI-TOF3
|
Purified protein
QC data |
1–2 weeks |
1 Listed is the typical turnaround time at GenScript. Please note that additional requirements may incur additional steps, and likewise longer processing time.
2 FREE Western QC data is available for tagged proteins that can be detected by the anti-His or anti-GST antibody. For other tagged proteins, additional charges may apply.
3 Optional MALDI-TOF data is available with additional charge.
This service can be expanded to include tag removal, endotoxin removal, and additional custom purification for a separate fee.
Quotations and Ordering:
 To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.
For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: protein@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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