Baculovirus Stock and Insect Cell Pellet Service
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The inconsistence of batch-to-batch virus titer is a common issue in protein expression using baculovirus-insect cell system, often resulting in a 1-2 week process of virus infectivity restoration. GenScript baculovirus stock and insect cell pellet preparation service offers the solutions to the problem, providing tittered, highly infectious and ready-to-use virus stocks with detailed storage protocols, and infected pellets SF-9, SF-21, and High-5 cells for immediate protein purification.
Our services are divided into a series of steps that can be performed either individually or in combination, including the entire protein expression process, from gene synthesis, cloning into baculovirus transfer vector, to recombinant baculovirus construction, high titer recombinant baculovirus production, and infected cell pellet preparation.
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Baculovirus Stock and Insect Cell Pellet |
Key features:
- Accurate virus titer determination with qPCR and/or plaque assay
- Delivery of ready-to-use and high titer virus stock
- Detailed virus storage protocols
- Infected cell pellets delivered ready for protein purification
Our capacities:
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Cell Culture/Month
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Working Virus
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Stock Virus
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Titration
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| Capacity |
500 L |
5 L |
2 L |
50 Assay |
| Concentration (pfu/ml) |
2x106 |
1x107 |
1x109 |
N/A |
Services Specifications:
| Services |
Description |
Required Materials | Deliverables | Timeline |
| Virus Amplification |
Virus amplification
Virus titration by qPCR |
5 ml virus stock |
Specified quantity of ready-to-use virus stock |
2 weeks |
| Virus Titration and infectivity Assay |
Virus titration by qPCR
Infectivity analysis by plaque assay |
2 ml virus stock |
3 independent qPCR results and plaque assay results |
1 week for viral particles, and 4 weeks for infectivity |
| Expression Optimization Service |
Virus amplification
Infection of Sf21, Sf9, and Hi5 lines with three MOIs
Cells harvested at 36, 48 and 72 hours post infection |
5-10 ml virus stock |
18 sets of cell pellets from 2-3 ml culture and media for protein yield evaluation |
2 weeks |
| Infected Cell Pellet Service |
Virus amplification
Infection of the chosen cell line
Cell harvest at optimized time point
Cell pellet preparation |
100 ml virus stock |
Cell pellets ready for protein purification and supernatant of secreted protein |
2 weeks (1-5 L),
4 weeks (5 - 20 L),
8 weeks (≥20 L) |
Quotations and Ordering:
 To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.
For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email for Quotation and Ordering: order@genscript.de |
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Ordering by Telephone: 001-732-885-9188 (USA)
Toll Free Tel. No.: 0-800-0006-320 (Germany) 0800-369-254 (Switzerland) |
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Fax: 001-732-210-0262 001-732-885-5878 |
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