Custom Polyclonal Antibody Services (Rat)
Services
Standard Protocol (Three animals)
| Steps |
Time from Project Initiation |
Time from Immunization |
Activity Description |
| Peptide synthesis |
≈2 weeks |
- |
- |
| Peptide conjugation to carrier |
≈3 weeks |
- |
- |
| Control serum collection |
≈4 weeks |
Day 0 |
Pre-bleed (≈0.1 ml serum) |
| Primary immunization |
≈4 weeks |
Day 1 |
0.1 mg per rat peptide-KLH conjugate, CFA, i.p. |
| 1st boost |
≈6 weeks |
Day 14 |
0.1 mg per rat peptide-KLH conjugate, IFA, i.p. |
| Test bleed and ELISA |
≈8 weeks |
Day 24 |
0.01-0.02 ml serum per rat. ELISA and initial response evaluation. |
| 2nd boost |
≈9 weeks |
Day 35 |
0.1 mg per rat peptide-KLH conjugate, IFA, i.p. |
| Test bleed and ELISA |
≈11 weeks |
Day 45 |
0.01-0.02 ml serum per rat. ELISA and initial response evaluation. |
| 3rd boost |
≈12 weeks |
Day 56 |
0.1 mg per mouse peptide-KLH conjugate, IFA, i.p. |
| Production bleed |
≈14 weeks |
Day 66 |
1.5 -2 ml serum per rat |
| ELISA and shipping |
≈15 weeks |
Day 80 |
Extend or terminate |
Quotation and Ordering:
To request a quotation, please download and complete our Quick Quotation Request, and send it to us by email or fax.
To place an order, please download and complete our Order Form, and send it to us by email or fax.
If submitting antigen, please mail it together with a completed hard copy of the Antigen Submission Form to Antibody Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.
If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our secure web server.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email for Quotation and Ordering: order@genscript.de |
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Ordering by Telephone: 001-732-885-9188 (USA)
Toll Free Tel. No.: 0-800-0006-320 (Germany) 0800-369-254 (Switzerland) |
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Fax: 001-732-210-0262 001-732-885-5878 |
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