PCR Cloning and Subcloning Timeline and Reagent Costs
PCR cloning and subcloning can be tedious and time-consuming. The typical time frame and cost, in reagents alone, of one PCR cloning project are outlined in the table below:
| |
Total |
Design |
Oligo Synthesis |
PCR |
Cloning |
Sequencing |
Maxi-Prep |
| Time (Day) |
13 |
1 |
3 |
1 |
3 |
3 |
2 |
| Cost (USD) |
295 |
|
25 |
20 |
100 |
100 |
50 |
The actual time and reagent cost can be much higher once other procedures, such as mutation correction and the screening of multiple vendors, are taken into account.
GenScript's PCR cloning and subcloning service can build your construct to your exact specifications in a similar time frame. We guarantee both the delivery itself and 100% fidelity to your specified sequence. Our constructs are delivered ready-to-use without any further subcloning required. Meanwhile, you and your staff can invest your time in creative research work. In addition, GenScript's proven reliability will allow you to plan your project with confidence and finance it with transparency.
To learn more about our PCR cloning and subcloning services...
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