E.coli Expression Guaranteed Package:
|
Steps: |
Deliverables: |
| Subclone:
Subclone the gene into 3 expression vectors
(Customer provides the gene or GenScript synthesizes
the gene for an additional fee) |
|
| Expression optimization and
verification:
1. Transformation of 3 constructs into bacteria
2. Expression optimization: induction time, temperature,
and IPTG concentration
3. Coomassie blue stained SDS-PAGE gel
4. Yield estimation and feasibility proposal for
large-scale expression (optional) |
|
| Expression and
purification:
1. Expression in 1 L bacterial culture
2. One-step affinity purification |
- 3 mg or more of purified soluble tagged protein
- QC data
|
Quotations and Ordering:
 To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our secure online messaging system.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.
For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
|
Email for Quotation and Ordering: order@genscript.de |
|
Ordering by Telephone: 001-732-885-9188 (USA)
Toll Free Tel. No.: 0-800-0006-320 (Germany) 0800-369-254 (Switzerland) |
|
Fax: 001-732-210-0262 001-732-885-5878 |
|
| Related Service & Products |
|
|
| Promotion |
 |
| |
