 GenScript Vector-Based miRNA Services
MicroRNAs (miRNAs) are small (~22 nucleotides) noncoding RNAs expressed by metazoans and viruses. They regulate target mRNAs posttranscriptionally by directing cleavage and degradation of the latter if there is full complementarity or by inhibiting translational initiation if complementarity is imperfect. miRNAs are processed from primary transcripts known as pri-miRNA, to short stem-loop structures called pre-miRNA, and finally to functional miRNA.
Several unique physical attributes of miRNAs, such as small size, lack of poly-adenylated tails, have made them elusive and challenging to study. With years of experience and expertise, GenScript has developed an advanced vector-based miRNA service, which can help to ease the difficulty. The resulting miRNA-expressing plasmid is purified and ready to transfect and can be used for a variety of applications to down-regulate gene expression.
Key Features:
- Comprehensive miRNA vector collection
- Custom cost-effective regenerated vectors
- Strong silencing effects
- Fast, reliable results at a competitive price
| Cat. No. |
Description |
Price per Construct |
SC1080 |
miRNA construction |
$500.00 |
Note: Maxiprep plasmid should be used for transfection. You may order MaxiPrep (> 100 µg) from GenScript starting from only $50.00.
GenScript's vector-based miRNA technology offers you an efficient way to clone your sequences of interest into any desired vector.
| Service Type |
Service Description |
Delivery Details |
Vector based pri-miRNA construction |
- Our optimized SV40 -CMV promoter causes increased expression of pre-miRNA
- Selection of cGFP fluorescent tags
- Final product is confirmed by stringent sequencing
|
- 4
μg of lyophilized plasmid DNA containing the desired miRNA construct
- Detailed sequencing data documenting the DNA sequence of each clone
- The typical
delivery time is about two weeks.
|
Vector-based pre-miRNA construction |
- Selection of cGFP fluorescent tags
- Final product is confirmed by stringent sequencing
|
Notes: GenScript's vector is completely compatible with adenovirus and lentivirus vector systems.
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Quotations and Ordering:
 For quotation requests and questions, you may contact us by email, phone, secured online quotation system.
Orders can be placed by phone, email, fax, or online with a formal PO
(Purchase Order) or credit card. Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
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