| Product Name |
GST Lysates |
Figures |
Species |
Human |
Document:
References:
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Baxter BK, et al. Atg19p ubiquitination and the cytoplasm to vacuole trafficking pathway in yeast. J. Biol. Chem. Nov 2005; 280(47): 39067-39076.
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Lacrue AN, et al. The novel Plasmodium gallinaceum sporozoite protein, Pg93, is preferentially expressed in the nucleus of oocyst sporozoites. Am. J. Trop. Med. Hyg. Sep 2005; 73(3): 634-643.
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Zhang XM, et al. Sec15 is an effector for the Rab11 GTPase in mammalian cells. J. Biol. Chem. Oct 8 2004; 279(41): 43027-43034.
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Tissue |
Other |
Description |
This product consists of 293 cells transfected with GST minigene, 26 kDa, 100 ug/100 ul. Human embryonic kidney (HEK) 293 cells were originally derived from human embryonic kidney tissue and subsequently demonstrated to be a useful cell type to produce adenoviruses, other vial vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of different fields. |
Storage |
Aliquot and store the product at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
Application |
Recommended dilutions: GST lysate is produced from 293 cells transfected with 26 kDa GST protein from Schistoma japonicum. It is delivered ready to load on SDS-PAGE gels for western blotting. 10 µg per lane is recommended for mini gels. |
