Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52 kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.
Concentration
200 µg of whole E. coli cell lysate (lyophilized)
Volume
Add 200 µl of dH2O to make the final concentration of 1 µg/µl in 1X SDS Loading Buffer. 0.02 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buffer.
Storage
Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
