Most site-directed mutagenesis is based on the PCR method, in which strand separation is accomplished by using a denaturing step to separate the complementary strands and in which efficient polymerization of the PCR primers is possible. In theory, the PCR method can be performed in the lab, but it requires considerable optimization, necessitates a staff skilled in both PCR and primer design. Often, the time and energy poured into in-lab PCR mutagenesis would be better spent elsewhere, such as on the lab's core creative work.

By outsourcing in vitro mutagenesis projects to GenScript your lab can profit from an extremely short turnaround time, even in demanding mutagenesis projects. At GenScript, we guarantee top accuracy and unlimited sites. Moreover, we offer comprehensive upstream and downstream services, including template sequencing, expression vector construction, and protein expression and purification. The proven expertise of GenScript's team in the field of in vitro mutagenesis combined with our optimized technology allows us to deliver mutated and verified vectors (purified DNA and bacterial clones) within only a few days, thereby saving scientists’ valuable time.

Quotations and Ordering:

Orders may be placed by email, phone, fax, or online with either a formal PO or credit card. We recommend that you submit a completed Mutagenesis Quotation/Order Form by email or via our secure online messaging system. Our customer service representatives are available 24 hours, Monday through Friday to assist you.

Please send your samples with full shipping information and PO number (if applicable) to Mutagenesis Service, GenScript, 120 Centennial Ave., Suite 105, Piscataway, NJ 08854, US.