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Custom Polyclonal Antibody Services (Rabbit)

1. SC1030: Partial Polyclonal Antibody Package (Rabbit)

Includes:
Delivery:
Time Frame:
  • Conjugation to KLH (optional)
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • 100-120 ml antisera from two rabbits
  • ELISA test
  • If the antigen is provided by the customer: 2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.
  • 1 ml of pre-immune serum
  • ELISA results
  • 100-120 ml of antisera from two rabbits
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • ELISA and Shipping: 1-2 weeks
  • Total 12-13 weeks

2. SC1247: Partial Antigen Affinity-Purified Polyclonal Antibody Package (Rabbit)

Service Package
Peptide Antigen
Protein Antigen
Includes
  • Conjugation to KLH
  • 2 rabbits, pre-immune bleed, 4 immunizations/rabbit
  • Final bleed
  • Peptide conjugated to affinity resin
  • Affinity purification of antibodies
  • ELISA test
  • Antigen provided by the customer: 7.5-8 mg of peptide (2.5-3 mg for immunization, 5 mg for purification)
  • Protein checking by SDS-PAGE
  • 2 rabbits, pre-immune bleed, 4 immunizations/rabbit
  • Final bleed
  • Protein conjugated to affinity resin (Dialyze the protein with PBS if needed)
  • Affinity purification of antibodies
  • ELISA test
  • Antigen provided by the customer: 7.5-8 mg of protein (2.5-3 mg for immunization, 5 mg for purification).The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution. Tris and other solution containing amino groups should not be used.
Delivery
  • 1 ml of pre-immune serum
  • ELISA results
  • Affinity-purified antibodies
  • remaining antigen
  • 1 ml of pre-immune serum
  • ELISA results
  • Affinity-purified antibodies
  • remaining antigen
Time Frame
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 13-14 weeks
  • Protein checking: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 13-14 weeks

3. SC1248: Partial Protein A-Purified Polyclonal Antibody Package (Rabbit)

Includes:
Delivery:
Time Frame:
  • Conjugation to KLH (optional)
  • 2 rabbits, pre-immune bleed, 4 immunizations/rabbit
  • Final bleed
  • Protein A purification
  • ELISA test
  • Antigen provided by the customer: 2.5-3 mg of peptide or 2.5-3 mg of protein is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.
  • 1 ml of pre-immune serum
  • ELISA results
  • Protein A-purified antibodies
  • remaining antigen
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 13-14 weeks

4. SC1015: Complete Peptide Polyclonal Antibody Package (Rabbit)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • 100-120 ml final bleed from two rabbits
  • ELISA test
  • 1 ml of pre-immune serum
  • ELISA results
  • 100-120 ml of antisera from two rabbits
  • 5mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • ELISA and Shipping: 1-2 weeks
  • Total 14-16 weeks

5. SC1045: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Rabbit)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune, four immunizations/rabbit
  • Final bleed
  • Protein A purification
  • ELISA test
  • 1 ml of pre-immune serum
  • ELISA results
  • Protein A-purified antibodies
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-17 weeks

6. SC1031: Complete Affinity-Purified Peptide Polyclonal Antibody Package (Rabbit)

Includes:
Delivery:
Time Frame:
  • Peptide synthesis (<15 a.a., >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • Final bleed
  • Peptide (5 mg) conjugated to affinity resin
  • Affinity purified antibodies
  • ELISA test
  • 1 ml of pre-immune serum
  • ELISA results
  • Affinity-purified antibodies
  • 5 mg of unconjugated peptide, MS and HPLC reports
  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-17 weeks

7. SC1044: Phospho-Specific Polyclonal Antibody Package (Rabbit)

Includes:
Delivery:
Time Frame:
  • Synthesis of two peptides, one with a modified amino acid (<15 a.a, >90% purity, 15 mg)and the other without modified amino acid (<15 a.a, >70% purity, 10 mg)
  • A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
  • Two rabbits, pre-immune bleed, four immunizations/rabbit
  • 100-120 ml antisera from two rabbits
  • Prepare two affinity columns
  • Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
  • ELISA test
  • 1 ml of pre-immune serum
  • ELISA results
  • Modified-specific antibodies from two rabbits
  • 5 mg of modified peptide and 5 mg of unmodified-peptide, MS and HPLC reports
  • Peptide Synthesis: 2-4 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification: 1 week
  • ELISA and Shipping: 1-2 weeks
  • Total 15-18 weeks

Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera or antibody products to prevent bacteria growth

Quotation and Ordering:

To request a quotation, please download and complete our Quick Quotation Request, and send it to us by email or fax.

To place an order, please download and complete our Order Form, and send it to us by email or fax.

If submitting antigen, please mail it together with a completed hard copy of the Antigen Submission Form to Antibody Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.

If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our secure web server.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Bestellung per Email
   Email for Quotation and Ordering: order@genscript.de
Bestellung per Telefon
   Ordering by Telephone: 001-732-885-9188 (USA)   
  Toll Free Tel. No.: 0-800-0006-320 (Germany)   0800-369-254 (Switzerland)
Bestellung per Fax
   Fax: 001-732-210-0262    001-732-885-5878