 GenScript Vector-Based siRNA Services
Vector-based siRNA has stronger in vivo inhibitory effects than
synthetic siRNA and can work with tissue-specific promoters. As
regarding stable cell lines can be developed, vector-based siRNA allows
researchers to observe the longterm knock-down effects of their target
genes. The inducible expression feature adds another layer of
flexibility. GenScript vector-based siRNA technology is an adaptation
of GenScript gene synthesis technology to the rise of RNA interference
(RNAi) as a powerful tool for gene function analysis and drug target
validation. With our acclaimed OptimumRNAiTM technology and siRNA design
tools, we can assist you to identify the most effective siRNA sequences with strong gene silencing effect, and clone into any cloning vectors you desire.
- Key Features
- Service Details
- Customer Testimonial
- Technical Resources
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| Cat. No. |
Description |
Number of Constructs |
Price per Construct |
SC1007 |
siRNA construction |
1–2 |
$425.00 ($500.00 for Lentiviral Vector) |
Note: More discount for batch orders (≥3). GenScript offers one-time fees vectors at only $225.00-$350.00 per vector.
Description:
A small DNA insert (about 70-80 bp) encoding a short hairpin RNA targeting the gene of interest is cloned into a siRNA expression vector. The insert-containing vector can be transfected into the cell, where it expresses the short hairpin RNA.
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- Promoter: Options include human U6 and H1 promoters.
- Vector antibiotic resistance: Options include neomycin, hygromycin, and zeomycin.
- Term: Poly (T) termination signal.
- BamH I and Hind III are used to clone the DNA insert into GenScript pRNA vectors.
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Protocol: Download  PDF file (U6 or H1 promoter), PDF file (CMV promoter).
Delivery:
Typical delivery: Includes a construct map, approximately 4 μg of lyophilized plasmid DNA containing the siRNA construct, and sequence data documenting the DNA sequence of each clone.
Quantity: 4 µg (MiniPrep) or 100 µg (MaxiPrep), and multi-milligram quantities are available.
*For MaxiPrep orders, we offer our endotoxin elimination service.
Quality Control: The insert is sequence-verified, and trace data is provided together with the plasmid.
Turnaround Time: About two weeks.
Storage: Store at -20 oC after delivery.
GenScript siRNA vectors are available separately.  |
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Customer Testimonial
"Your constructs have provided us with the ability to make exciting progress in understanding the causes of brain developmental disease."
― Dr. Richard Vallee, Columbia University Medical Center, USA
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The GenScript siRNA technology package includes siRNA design, siRNA vectors, and custom siRNA construction. GenScript is proud to offer a selection of proven siRNA plasmid vectors. If you have any question about our siRNA services and products, please click the following links:
RNAi Technology
Frequently Asked Questions: Vector-Based siRNA |
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Quotations and Ordering:
For quotation requests and questions, you may contact us by email, phone, secured online quotation system.
Orders can be placed by phone, email, fax, or online with a formal PO
(Purchase Order) or credit card. Our customer service representatives are available 24 hours Monday through Friday.
You may contact us anytime for assistance.
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