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ONE-HOUR WesternTM Kit Accessories

GenScript is pleased to provide reagents and other accompanying products for its line of ONE-HOUR WesternTM Kits, including detection substrates, solutions, membranes, PAGE gels, and labware.

The detection substrates include Chemiluminescent HRP Substrate and ChromoSensor TMB Substrate. The solutions are wash buffer, pretreat solutions, A&G blocker, and stripping solutions. The PAGE gel is our ExpressTM Precast Gel. The labware are forceps, dot boxes, and wash boxes.

Example:

1. Shown below is a western blot detection of housekeeping gene product GAPDH protein using LumiSensorTM Chemiluminescent HRP Substrate Kit.
Western blot using the HRP substrate Figure 1. 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit.

2. Western blot wash box, dot blot box, and forceps.
western blot forceps, wash box, dot blot box

Products:


GenScript ExpressTM PAGE Gels

ONE-HOUR Western Detection Substrate

 Cat. No. Name Size Price Quantity
L00225
 LumiSensorTM Plus Chemiluminescent HRP Substrate Kit 1 Kit (2×30 ml) $89.00
L00221V60 LumiSensorTM Chemiluminescent HRP Substrate Kit 1 Kit (2×30 ml) $55.00
L00221V300 LumiSensorTM Chemiluminescent HRP Substrate Kit 1 Kit (2×150 ml) $145.00
L00221V500 LumiSensorTM Chemiluminescent HRP Substrate Kit 1 Kit (2×250 ml) $235.00
L00222V60 ChromoSensorTM One-Solution TMB Substrate 1 pk (60 ml) $28.00
L00222V125 ChromoSensorTM One-Solution TMB Substrate 1 pk (125 ml) $39.00
L00222V250 ChromoSensorTM One-Solution TMB Substrate 1 pk (250 ml) $68.00
L00222V500 ChromoSensorTM One-Solution TMB Substrate 1 pk (500 ml) $119.00
L00354 LumiSensorTM Super HRP Substrate 1 kit $ 135.00


Western Solutions

Cat. No. Name Size Price Quantity
MB01011 10X Wash Solution 100 ml $65.00
M01014 A&G Blocker (100 X) 1 ml $20.00
M01013 Pretreat A (100 ml) & B (100 ml) 1 pack $145.00
M01015 Stripping Solution 100 ml $29.50
L00354 LumiSensorTM Super HRP Substrate 1 kit $ 135.00


ONE-HOUR WesternTM Kit Labware

 Cat. No. Name Size Price Quantity
M00100 Western Wash Box (Clear) 1 pack of 10 $80.00
M00101 Western Wash Box (Blue) 1 pack of 10 $80.00
M00102 Western Wash Box (Yellow) 1 pack of 10 $80.00
M00103 Western Wash Box (Black) 1 pack of 10 $80.00
M00108 Dot Blot Box (Clear) 1 pack of 10 $98.00
M00109 Dot Blot Box (Blue) 1 pack of 10 $98.00
M00110 Dot Blot Box (Yellow) 1 pack of 10 $98.00
M00099 Western Membrane Forceps 1 pack of 10 $50.00

WestClear TM Nitrocellulose Membrane

 Cat. No. Name Size Price Quantity
L00224A60 WestClearTM Nitrocellulose Membrane 1 pk (10 sheets of 7.5 x 8 cm)$ 60.50
L00224A225 WestClearTM Nitrocellulose Membrane 1 pk (5 sheets of 15 x 15 cm) $ 75.90