One-Step WesternTM Basic Kits
One-Step WesternTM Basic Kits are the simplified version of One-Step WesternTM Complete Kits. The WestClearTM
Nitrocellulose Membrane and LumiSensorTM Chemiluminescent HRP Substrate Kit are not included in the package.
It is economical to choose these kits if you have surplus accessories in hand. Associated with GenScript's
accessories, One-Step WesternTM Basic Kits can perform as excellent as One-Step WesternTM Complete Kits.
To obtain best results and reduce undesirable variations such as high background, we strongly recommend that the following reagents be used with One-Step WesternTM Basic Kit:
- GenScript WestClearTM Nitrocellulose Membrane (L00224A60).
- GenScript LumiSensorTM Chemiluminescent HRP Substrate Kit (L00221V60).
- GenScript ChromoSensorTM One Solution TMB Substrate (L00222V60).
Key Features:
- Easy to perform: Fewer steps mean fewer chances for human error.
- High sensitivity: The kit's sensitivity is comparable to or better than that of the classical 4.5-hour procedure, depending on the quality and amount of antibodies used.
- Reproducible results: The kit produces highly reproducible results.
- Far less optimization than the classical three-step method.
- Secondary antibody is included.
Applications:
- Protein/antigen detection
- Evaluation of protein expression
- Antibody/antigen titration
- Analysis and comparison of primary antibody activity levels
Technical Manual:
Download PDF file TM0352
Product List:
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