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BacPower™ Guaranteed Bacterial expression packages

BacPower™ Guaranteed Bacterial expression packages

Our BacPower™ Guaranteed Bacterial expression packages now offer a comprehensive panel of options for your target protein production in bacterial expression system with guaranteed protein amounts, purities, endotoxin levels and the option of producing tag-free proteins as well, to meet a variety of your research needs. You simply provide protein sequence and we will deliver the protein (tagged or tag-free) at the amount and purity that you specified. All BacPower™ guaranteed bacterial expression packages start with our proprietary codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our bacterial expression system.  If your target recombinant protein becomes insoluble in the inclusion body fraction, FoldArt™, our proprietary protein refolding technology will be applied to solubilize the protein at no extra cost to you. In case we fail on either the amount or purity as specified, there is no charge to you whatsoever.  Place your order today to experience unprecedented, high quality service yet competitive pricing, exclusively offered with our BacPower™ Guaranteed Bacterial expression packages.

BacPower™ Guaranteed Bacterial Expression packages with unprecedented assurances

  • Gene synthesis is included in the package – no additional cost to you.
  • Guarantees the amount of custom recombinant protein – no cost to you if project fails.
  • Guarantees the purity of custom recombinant protein – no cost to you if desired purity is not achieved.
  • Competitive price - starting from $2,200.
  • Fast turnaround time - as little as 6 weeks.
Customer Provides
Tag Options
Amount*
Timeline
Purity Options
Endotoxin Level Options
Pricing
Target protein sequence
Small Tag (e.g. His)
3mg - 6mg
6-8 weeks
75%
85%
90%
≤1.0 EU/μg
≤0.1 EU/μg
≤0.01 EU/μg
Starting from $2,200
Large Tag (e.g. GST)
5mg - 10mg
Tag-Free
5mg - 10mg
9-10 weeks
Any combination of protein amount, purity and endotoxin level can be chosen from this list
*Protein requests greater than 15mg will be treated as custom protein production requests and they will not be eligible for the guarantees listed on this page

Terms and Conditions

  • Protein sequences are subject to pre-project analysis.
  • Exclusions are applicable to membrane proteins, toxic proteins and for target proteins greater than 100kDa.
  • To increase the expression level of your protein, codon optimization using our proprietary, OptimumGene™ technology, will be performed during gene synthesis.
  • Insoluble proteins maybe subject to FoldArt™ technology, GenScript's proprietary protein refolding platform.
  • In all cases, final protein will be guaranteed to meet customer specifications, failing which, any charge to the customer will be waived.

Package Details

Steps Specification Timeline* Deliverables

Gene synthesis and Sub cloning
  • Gene synthesis and Codon optimization using our proprietary platforms
  • Sub cloning into appropriate expression vector
  • DNA QC
2-3 weeks
  • Protein in buffered solution with your specified amount & purity – Guaranteed
  • Optimized gene sequence report
  • QC data

Transformation and Expression testing
  • Transform plasmids into the appropriate bacterial expression strain followed by expression evaluation and optimization
0.5 week

Large scale protein purification
  • Scale up expression using optimized conditions followed by purification to reach desired protein amounts and purity
1-2 weeks

Refolding (if required)
  • Refolding in case of insoluble protein, using high-hydrostatic-pressure techniques, small-molecule additives, and column-refolding methods
0.5-1 week

Tag removal and MALDI-TOF
(if required)
  • Tag removal by digestion with suitable protease followed by separation of tag free protein. Tag free protein confirmed by MALDI-TOF
2 weeks

QC & Quantitation
  • Purity analysis and Detection using SDS-PAGE and Western Blot (for tagged protein)
  • Bradford assay for quantitation
0.5 week

*All timelines are approximate. In most cases, we deliver within 6 weeks, however, optional steps might prolong the overall timeline slightly

Quotations and Ordering

@First time users will have to create a GenScript account before they can login
#Automatic Quote will be generated only if sequences qualify for the Guaranteed service, as determined by our proprietary sequence analysis platform, failing which, our Technical Account Manager will contact you with a custom protein production quote. Please be assured that our custom services are equally high quality and competitive as our Guaranteed services

For any other inquiries, please contact us using the details listed below. Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.