Home » Discovery Biology Pharmaceutical Services » In Vitro Pharmacology » Stable Cell Line (CellPower™) for Assay Development
Email to GenScript

Stable Cell Line
Stable Cell Line

Stable Cell Line Services for Assay Development

Recombinant stable cell lines are one of the widely used tools in drug discovery, toxicity testing, and basic research. Long-term stable expression of a gene of interest (GOI) is usually achieved by transfection or viral transduction of a vector containing the expression cassette of the GOI together with a selection marker (antibiotics or fluorescent proteins). In contrast to transient expression, stable cell line offers reproducible results for assay studies. Read More »

Stable Cell Line Services: Advantages working with GenScript CellPower™

  • Multiple gene transfer methods available: Both virus (lentivirus or retrovirus) and non-virus (chemical or physical) based options
  • Track record of handling difficult-to-transfect cell lines
  • Experience in stably expressing membrane proteins
  • High quality standards for mycoplasma control and detection
  • Guaranteed mRNA expression
  • Multiple functional assay methods for clone validation
  • One-stop solution for drug discovery: From gene synthesis to cell development
  • LentiCRISPR technology licensed from Broad Institute, Inc. Cambridge, Massachusetts

Stable Cell Line Development services by CellPower™

  • Classic CellPower™
  • CellPower™ Plus
  • Our Experience
  • Case Study
Service Content Service Packages Deliverables Turnaround Time
Recombinant Expression(Over-Expression, Inducible Expression, Knock-Down Expression) Stable Pool (SC1394A)
2 vials of stable pool, Certificate of Analysis (COA) report
7-17 weeks
Single Clone (SC1394B)
2 single clones, 2 vials/clone (1×106 cells/vial), Certificate of Analysis (COA) report
12-30 weeks
Lenti-CRISPR Knock-Out Expression Stable Pool (SC1652-V)
2 vials of stable pool, Certificate of Analysis (COA) report
7-17 weeks
Single Clone (SC1652-V)
2 single clones, 2 vials/clone (1×106 cells/vial), Certificate of Analysis (COA) report
12-30 weeks
Service Specifications Deliverables Timeline
CellPower™ Plus
Cell pool: Use new QC standard covering more than 160 species of mycoplasma
qPCR validated stable pool
Detailed report with QC data
11~13 weeks
(3 months)
Single cell clone: Use new QC standard covering more than 160 species of mycoplasma
2 vials of cells, 1×106 cells/vial. qPCR validated
Detailed report with QC data
15~19 weeks (4~5months)
Pre-QC: GenScript will use the new QC standard to exclude potential mycoplasma contamination in host cells
QC report
2~3 weeks
our experiences

Over-expression of GOI

our experiences   our experiences

Figure 1. PD1 expression by FACS analysis (left) and binding assay (right) in CHO-K1/PD1 cells.

Knock-down Expression of GOI

our experiences   our experiences

Figure 2. Validation of knockdown efficiency via western blotting (left) and functional assay (right).

Inducible expression of GOI

our experiences   our experiences

Figure 3. Q‐PCR (left) and western blotting (right) validation of Target A expression in HepG2 cells.

LentiCRISPR KO

our experiences
our experiences

Figure 4. Knockout of Target B in HepG2 cells validated by sequencing (above) and western blotting analysis (below).

Workflow of Our CellPower™ Stable Cell Line Development Process

Stable Cell Line Services for Assay Applications

Engineered Stable Cell Line Products

GenScript offers a wide selection of Immune Checkpoint Stable Cell Lines and GPCR Stable Cell Lines to accelerate your drug discovery. See more product introduction.

Also available are stable cell lines for protein production. See our Production Grade Stable Cell Line webpage for more details.

Quotation and Ordering

  • Please contact us mentioning "Discovery Biology", our customer service representatives are available 24 hours a day, Monday through Friday to assist you with Form preparation.