You are here: Biology CRO for Drug Discovery » Bio-Assay Center » Guaranteed Stable Cell Line
GenScript Share

guaranteed lentiviral platform

Development of Stable Cell Line expressing gene of interest has been the ever-demanding prerequisite for drug discovery. However, you might've got caught in the following situations of making cell lines:

  • Repetition of plasmid preparation, low transfection efficiency, tedious clone picking
  • Unable to transfer your gene of interest into certain cell lines
  • Worried about missing "cell growth phase"
  • Limited by gene size (up to 2kb) in cloning and gene transfer

GenScript's promises:

  • Delivery or no fee
  • Mycoplasma-free cells guaranteed
  • Any genes and any host cell lines customized with your own project
  • 100% success of developing over 200 stable cell lines in the past 4 years
  • Guaranteed expression of your genes validated by specific screening methods designated for your proteins

CellPower™: Guaranteed Stable Pool or Single Cell-derived Clones

Service Steps Service Features Additional Validation Services

Subcloning of gene of interest

    Most affordable price
  • $2490 for stable pool
  • $4990 for single cell-derived clones

Membrane receptors: FACS, ELISA & Western Blot

Plasmid preparation

GPCRs: Ca2+, cAMP, IP-1, radiometric binding assays

Lentivirus packaging and production

Ion Channels: electrophysiology,
Transporters: radiometric assay

Transduction & selection

    Fast turnaround
  • 8-10 weeks for stable pool
  • 12-16 weeks for single cell-derived clones

Proteases, kinases, and other enzyme:
substrate conversion assay

Q-PCR validated single(poly)-clone

Nuclear receptors: reporter gene assays

Deliverables: 2 vials of cells,
3X106 cells each vial

Cytokines: proliferation or other relevant assays


Cases

  • GenScript's cell hosts at your command GenScript's cell hosts
  • Knockdown GOI expression Knockdown GOI expression
  • Over-expression of IL7-R and positive control Over-expression of CRLF2 and IL7-R

Conventional method for gene transfer

Besides lentivirus-based gene transfer, GenScript also provides non-viral gene transfer platform, which enables you:

  • Transfer any gene in any form (oligonucleotides, RNA, or DNA) into common cell lines
  • Conduct both transient expression and stable expression
  • Obtain desired cell lines at the most affordable price

Redirect to Conventional Gene Transfer


Quotation and Ordering:

CellPower Service QuotationCellPower Service To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.

Monoclonal Antibody Sequencing Service To order, please download and complete the Quotation/Order Form and send it to us by email or fax with a formal PO (Purchase Order) or credit card information. You can also submit PO/credit card information by phone or via our Secure Online Messaging System.

Monoclonal Antibody Sequencing Service If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Bioassay Department, GenScript USA Inc., 860 Centennial Avenue., Piscataway, NJ08854, US.

Monoclonal Antibody Sequencing Service For questions, or to inquire about the status of your order contact us by email, phone, fax or via our Secure Online Messaging System.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.


Email: bioassay@genscript.com
Phone: 1-877-436-7274 (Toll-Free), 1-732-885-9188
Fax: 1-732-210-0262, 1-732-885-5878