His tagged protein detection & purification
His-tag is one of the most widely applied tags for recombinant protein expression and purification. It is also used for binding assay to detect protein-protein interactions by a pull-down assay or in conjunction with anti-His antibody as IP/Co-IP assay. The three new high quality mAb based assay products will provide convenient, easy, and robust tools for different purposes, including purification and detection of His-tagged fusion-protein for downstream assays, enrichment and screening of His-tagged fusion-protein mutants, and Co-IP & screening of interacting proteins.
His Tag ELISA Detection Kit
GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatant. This kit also offers a His-tagged protein stock for customer recovery verification assay.
Detect His-tagged proteins.
His Tag Antibody Plate
His Tag Antibody Plate is a microtiter plate coated with THE™ Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.
Other Tagged Protein Detection & Purification
DYKDDDDK(Flag) Tag Antibody Plate (96-well)