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His tagged protein detection & purification

His-tag is one of the most widely applied tags for recombinant protein expression and purification. It is also used for binding assay to detect protein-protein interactions by a pull-down assay or in conjunction with anti-His antibody as IP/Co-IP assay. The three new high quality mAb based assay products will provide convenient, easy, and robust tools for different purposes, including purification and detection of His-tagged fusion-protein for downstream assays, enrichment and screening of His-tagged fusion-protein mutants, and Co-IP & screening of interacting proteins.

His Tag ELISA Detection Kit

GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and semi-quantitation using a standard of His-tagged protein of known molecular weight (M.W.). This kit contains all reagents and buffers required for His tag detection, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatants. This kit is based on competitive ELISA, where a His-tagged protein (M.W. 12.7kD) is pre-coated on the microwell plate. This kit also offers a His-tagged protein stock for customer recovery verification assay.

Applications:

Detect His-tagged proteins.
Screen optimal expression conditions.
Semi-quantitation with known His-tagged protein as standards.

Example

Conc. of His Tag Standard		OD₄₅₀
(ng/ml)	(pmol/ml)	Duplicate 1	Duplicate 2	Average
0	0	2.177	2.183	2.18
1	0.088	1.833	1.831	1.832
3	0.265	1.374	1.418	1.396
9	0.796	0.825	0.922	0.874
27	2.389	0.516	0.444	0.48
81	7.168	0.266	0.289	0.278
243	21.504	0.158	0.157	0.158
729	64.513	0.105	0.101	0.103

Typical assay results and standard curve using His Tag ELISA Detection Kit

His Tag Mini Purification and Detection Kit

GenScript His-Tag Mini Purification and Detection Kit is developed for the efficient small scale purification or immunoprecipitation (IP) and detection of His-tagged proteins in eukaryotic and prokaryotic expression systems. Superparamagnetic beads pre-charged with nickel are ready for quick binding of His-tagged proteins. The Magnetic stand in the kit facilitates Magbeads isolation. After purification/IP, His tagged protein could be detected by THE™ His tag monoclonal antibody (mAb) included in the system. This kit also offers two His tagged proteins (26.1kDa and 51.6kDa, respectively) as positive control and general molecular weight (M.W) ladder for Western blot detection.

Applications:

Purification and detection of His-tagged proteins under native conditions & denaturing conditions.
Screen His-fusion protein expression for expression optimization.
One-stop purification and detection service with Ni-charged magnetic beads and industry premium anti-his antibody.

His Tag Antibody Plate

His Tag Antibody Plate is a microtiter plate coated with THE™ Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Cat. No.	Product Name	Size	Price
L00440b	His Tag Antibody Plate	5 plates (black, 96 wells)	$179.00
L00440c	His Tag Antibody Plate	5 plates (clear, 96 wells)	$179.00
L00440w	His Tag Antibody Plate	5 plates (white, 96 wells)	$179.00

Other Tagged Protein Detection & Purification

DYKDDDDK(Flag) Tag Antibody Plate (96-well)
DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THE™ Anti DYKDDDDK (Flag) Tag Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG, such as anti mouse IgG antibodies and protein A.

Cat. No.	Product Name	Size	Price
L00455B	DYKDDDDK(Flag) Tag Antibody Plate (Black, 96-well)	5 plates	$229.00
L00455C	DYKDDDDK(Flag) Tag Antibody Plate (Clear, 96-well)	5 plates	$229.00
L00455W	DYKDDDDK(Flag) Tag Antibody Plate (White, 96-well)	5 plates	$229.00

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