Mouse Anti-DYKDDDDK-tag Monoclonal Antibody, IgG1 Subclass
*This product has been discontinued! *
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Mouse Anti-DYKDDDDK-tag Monoclonal Antibody, IgG1 Subclass  |
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| Full Name |
Mouse Anti-DYKDDDDK-tag Monoclonal Antibody, IgG1 Subclass
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Abbreviated name-1 |
Mouse Anti-DYKDDDDK-tag mAb; |
| Documents |
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 Document-MSDS: 933_20051003104711.PDF (PDF) |
| Figures |
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| Reference |
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Schuster M, et al. Protein expression strategies for identification of novel target proteins. J Biomol Screen. Apr 2000; 5(2): 89-97.
Nielsen UB, et al. Targeting of bivalent anti-ErbB2 diabody antibody fragments to tumor cells is independent of the intrinsic antibody affinity. Cancer Res. Nov 2000; 60(22): 6434-6440.
Kimura M, et al. Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Mol Cell Biol. Mar 2002;22(5):1577-1588.
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Description |
Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a purified IgG1/kappa monoclonal antibody isolated from murine ascites fluid that binds to proteins with a DYKDDDDK marker at a free N-terminus or C-terminus. It is useful for the identification of proteins with DYKDDDDK markers at free N-termini and C-termini by common immunological applications in yeast, animal, and E. coli cells. GenScript Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is also useful for affinity purification of proteins with a DYKDDDDK marker at free N- termini or C-termini when bound to a solid support. |
Ig Subclass |
Mouse IgG1/kappa |
Storage |
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Stored at 4°C, the aliquot will remain stable for three months. |
Specificity |
Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins. |
Immunogen |
A synthetic peptide (DYKDDDDK) coupled to KLH |
Reconstitution |
Lyophilized from 1 mg/ml PBS solution. Reconstitute it with sterile water to 1 mg/ml. |
Applications |
Western blot: 1:1,000
Immunoprecipitation: 1:200
Immunostaining: 1:200 |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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