| Full Name |
Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb
|
Abbreviated name-1 |
Goat Anti Rabbit IgG (H&L) [HRP] |
|
AliasAbbreviated Name-2 |
Anti Rabbit IgG (H&L) [HRP] |
Description |
GenScript Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with rabbit IgG heavy and light chains. |
Ig Subclass |
Goat IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
This antibody specifically recognizes rabbit IgG and other species are not tested. |
Concentration |
1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Full-length rabbit IgG (purified) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml. |
Applications |
GenScript Goat Anti-Rabbit IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:50,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized |
Conjugation |
Horseradish peroxidase (HRP) |
Species Reactivity |
Rabbit |
Host Species |
Goat |
Antigen Species |
Rabbit |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
|
![Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb](/images/cgi2007/table2.gif "Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb"){kind=small}
![Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb](/images/download_PDF.jpg "Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb")
MSDS: 20090202211706 (PDF)