Fibrillarin Antibody, mAb, Mouse
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Fibrillarin Antibody, mAb, Mouse  |
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| Full Name |
Fibrillarin Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-Fibrillarin mAb |
| Documents |
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 MSDS: 20081020222132 (PDF) |
| Figures |
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| Reference |
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Tam R, et al. The 4q subtelomere harboring the FSHD locus is specifically anchored with peripheral heterochromatin unlike most human telomeres. J. Cell Biol. Oct 2004; 167(2): 269-279.
Partridge JJ, et al. DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP. Mol. Biol. Cell. Oct 2003; 14(10): 4221-4229.
Pollard KM, et al. Proteolytic cleavage of a self-antigen following xenobiotic-induced cell death produces a fragment with novel immunogenic properties. J. Immunol. Aug 2000; 165(4): 2263-2270.
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AliasAbbreviated Name-2 |
Anti-Fibrillarin |
Description |
Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of two extended beta-sheets flanked by four alpha-helices. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34,000 Da protein, which has been subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus. |
Storage |
This product will remain stable for one year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquots will remain stable for three months if stored at 4°C. |
Specificity |
This antibody cross-reacts with fibrillarin in human, rat, plant, drosophila, C. elegans and S. pombe. |
Concentration |
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid |
Clone |
A00153.01 |
Immunogen |
This antibody is raised against yeast fibrillarin, a highly conserved nucleolar protein present in small nucleolar ribonucleoprotein (snoRNP) complexes. |
Applications |
Western blot of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL)
Other (non-ECL) Western detection methods: 1:500 to 1:2,500
Detection mammalian fibrillarin on Western blot by ECL: 1:250
Immunofluorescence on yeast cells: 1:500 to 1:2,500
Immunofluorescence of mammalian cells: 1:250 |
Conjugation |
Unconjugated |
Species Reactivity |
Human, rat, plant, drosophila, C. elegans and S. pombe |
Host Species |
Mouse |
Antigen Species |
Yeast |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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