| Full Name |
Goat Anti-Human IgG Antibody (H&L) [HRP], pAb
|
Abbreviated name-1 |
Goat Anti Human IgG (H&L) [HRP]; Anti Human IgG (H&L) [HRP] |
|
Description |
This polyclonal goat antibody is purified by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It binds to both the heavy and light chains of human IgG. |
Ig Subclass |
Goat IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Concentration |
1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal |
Immunogen |
Full length human IgG (purified) |
Purification Method |
Immunoaffinity chromatography |
Applications |
GenScript Goat Anti-Human IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000.
Western blot: 1:5,000-1:50,000
Other applications: user-optimized |
Conjugation |
Horseradish Peroxidase (HRP) |
Species Reactivity |
Human |
Host Species |
Goat |
Antigen Species |
Human |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
|
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Document-MSDS: 4174_20080228022831.PDF (PDF)