| Full Name |
Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb
|
Abbreviated name-1 |
Donkey Anti-Goat IgG (H&L) [HRP] |
|
AliasAbbreviated Name-2 |
Anti-Goat IgG (H&L) [HRP] |
Description |
Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody is highly purified from donkey antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with goat IgG heavy and light chains. |
Ig Subclass |
Donkey IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Concentration |
1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal. |
Immunogen |
Full-length goat IgG (purified) |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with 1 ml of deionized water (or equivalent) to antibody concentration of 1 mg/ml. |
Applications |
Donkey Anti-Goat IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized |
Conjugation |
Horseradish peroxidase (HRP) |
Species Reactivity |
Goat |
Host Species |
Donkey |
Antigen Species |
Goat |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
|
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MSDS: 20080228012329 (PDF)