MBP-tag Antibody, mAb, Mouse
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MBP-tag Antibody, mAb, Mouse  |
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| Full Name |
MBP-tag Antibody, mAb, Mouse
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Abbreviated name-1 |
MBP-tag Monoclonal Antibody; |
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Description |
Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP tagged protein is purified with an amylose column. The MBP tagged protein can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein. MBP-tag Antibody, mAb, Mouse is suitable for detecting fusion proteins that contain a MBP-tag.
GenScript MBP-tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified recombinant MBP fusion protein. |
Ig Subclass |
Mouse IgG1 |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript MBP-tag Antibody, mAb, Mouse specifically reacts with fusion proteins containing MBP epitope tags. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Purified recombinant MBP fusion protein |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges
are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.5-1 µg/ml µg/ml |
Fusion Partner |
Spleen cells are fused to SP2/0-Ag14 mouse myeloma cells. |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Document-MSDS: 3739_20080118034804.PDF (PDF)