THETM His Tag Antibody [HRP], mAb, Mouse
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THE TM His Tag Antibody [HRP], mAb, Mouse  |
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![THE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse](/images/cgi2007/table2.gif "THE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse")
| Full Name |
THETM His Tag Antibody [HRP], mAb, Mouse
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Abbreviated name-1 |
His-tag Antibody [HRP],mAb, Mouse; |
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Description |
GenScript THETM His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays. |
Ig Subclass |
IgG1, k |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
Immunogen |
A synthetic peptide HHHHHH coupled to KLH |
Purification Method |
Protein A affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
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Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
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Conjugation |
Horseradish Peroxidase (HRP) |
Host Species |
Mouse |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Document-MSDS: 3891_20080213022807.PDF (PDF)![THE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse](/ssl-bin/site2/show_image.cgi?op=3375_20070711043948.JPG "THE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse")