THETM beta Actin Antibody, mAb, Mouse
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THE TM beta Actin Antibody, mAb, Mouse  |
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| Full Name |
THETM beta Actin Antibody, mAb, Mouse
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Abbreviated name-1 |
cytoplasmic 1 antibody |
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Description |
β-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to ß-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton. |
Key Features |
GenScript THETM beta Actin Antibody, mAb, Mouse has high sensitivity, low background in WB compared with antibody on the market. |
Ig Subclass |
IgG2a, k |
Storage |
THETM beta Actin Antibody, mAb, Mouse should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles. |
Specificity |
THETM beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig. It has not yet been tested in other species. |
Concentration |
40 µg, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Note: 100 µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA
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Clone |
2D1D10 |
Immunogen |
A synthetic peptide DDDIAALVVDNGSG coupled to KLH |
Purification Method |
Protein G affinity column |
Reconstitution |
Reconstitute the lyophilized antibody (40 μg or 100 μg) with 80 μl or 200 μl of deionized water to the final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0 µg/ml
Other applications: user-optimized
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Fusion Partner |
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells |
Conjugation |
Unconjugated |
Species Reactivity |
Mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig |
Host Species |
Mouse |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
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MSDS: 20080228041201 (PDF)
